Application of Collagen Hydrolysate Extracted from Limed Hide Waste in the Crop Grow Seedlings

2021 ◽  
Vol 43 (5) ◽  
pp. 546-546
Author(s):  
Li Zhao Li Zhao ◽  
Yanru Long and Haibin Gu Yanru Long and Haibin Gu
Keyword(s):  
Chlorophyll Content ◽  
Inorganic Nitrogen ◽  
Germination Rate ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Plant Weight ◽  
Molecular Weights ◽  
Collagen Hydrolysates ◽  
Nutrient Solutions

In this work, the collagen hydrolysates with different molecular weights were successfully extracted from the limed hide waste, and used at the nutrient solutions for crop breeding. Firstly, using the single factor and orthogonal exp eriments, hydrolytic process parameters of limed hide waste were optimized for the Alcalase-based enzymatic and Ca(OH)2-based alkali, and alkali-enzyme methods that led to the corresponding collagen hydrolysates with different molecular weights. The obtained collagen hydrolysates were characterized by gel permeation chromatography (GPC), amino acid analysis, the inductively coupled plasma optical emission spectrometer (ICP-OES), and Kjeldahl method. Then, the collagen hydrolysates were used as organic nitrogen sources to prepare fertilizers for the grow seedlings of Triticum aestivum, Glycine max, and Brassica napus, and inorganic nitrogen solutions were used as controls. The effects of these nutrient solutions on the seedlings and growth of the three crops were investigated. Concretely, the germination rate, plant weight, seeding height, soluble sugar content, and chlorophyll content were tested. Results indicated that all the collagen hydrolysate products could be used to prepare water-soluble fertilizers that can intensively boost germination, plant weight, and seeding height, and greatly increase soluble sugar and chlorophyll content in leaves. Furthermore, the fertilizer efficiencies of all the tested degradation products are much better than that of the water-soluble fertilizer containing inorganic nitrogen. All the proteolytic nutrient solutions with different molecular weights can promote the growth of crops, but the smaller the molecular weight is, the better the growth effect of crops is, which is manifested in the higher germination rate, plant weight, seedling height, soluble sugar content and chlorophyll content of seeds.

scholarly journals Toxicity evaluation of collagen hydrolysates from chrome shavings and their potential use in the preparation of amino acid fertilizer for crop growth

2022 ◽  
Vol 4 (1) ◽  
Author(s):  
Li Zhao ◽  
Shengdong Mu ◽  
Weixiang Wang ◽  
Haibin Gu
Keyword(s):  
Amino Acid ◽  
Resource Utilization ◽  
Growth And Development ◽  
Germination Rate ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Agricultural Field ◽  
Promoting Effect ◽  
Collagen Hydrolysates

AbstractResource utilization of chrome shavings (CS) has attracted a lot of attention from scientists and technologists in leather industry. Especially, the collagen hydrolysates extracted from CS are expected to find potential application values in agricultural field. However, there is no biotoxicity analysis of collagen hydrolysates from CS. Herein, the collagen hydrolysates with different molecular weights were produced from CS by three hydrolysis dechroming methods including alkaline hydrolysis, enzymatic hydrolysis and alkaline-enzymatic synergistic hydrolysis, and the optimal hydrolysis process of CS was designed and conducted. To evaluate their toxicity, the three collagen hydrolysates were formulated into a nutrient solution for zebrafish development. The obtained results indicated that the hydrolysates with low concentrations (less than 0.6 mg/mL) were safe and could promote the development for zebrafish embryos. Furthermore, the three collagen hydrolysates were utilized as organic nitrogen sources and formulated into amino acid water-soluble fertilizers (AAWSF) including alkaline type fertilizer (OH), enzymatic type fertilizer (M) and alkaline-enzymatic type fertilizer (OH–M) for the early soilless seeding cultivation of wheat, soybean and rapeseed. It is worth mentioning that the chromium contents in the prepared AAWSF were less than 10 mg/kg, which is far less than the limit value in the standard (China, 50 mg/kg). The growth and development of seedlings (germination rate, plant height, fresh weight of leaves, soluble sugar content and chlorophyll content) were investigated. The corresponding results showed that the growth of seedlings watered with AAWSF was better compared with the other treatments, and the OH–M fertilizer had the best promoting effect on the seedlings growth and development, followed by the M and OH fertilizers. The safe toxicity assessment of the collagen hydrolysates will expand their application scope, and the use of collagen hydrolysates extracted from CS for seedlings growth also provides an effective and reasonable way to deal with the chromium-containing leather solid waste, which is an effective way to realize its resource utilization. Graphical Abstract

The Influence of Chlorophenoxy Herbicides MCPA on Creeping Bentgrass Physiological Index

2011 ◽  
Vol 356-360 ◽  
pp. 2763-2766 ◽  
Author(s):  
Yang Fan Gao ◽  
Ming Wang Shi ◽  
Jian Hua Wang
Keyword(s):  
Chlorophyll Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Creeping Bentgrass ◽  
Physiological Index ◽  
Soluble Sugar Content ◽  
Content Determination ◽  
Chlorophenoxy Herbicides ◽  
Test Result

In this article, we studied to different concentrations of MCPA to creeping bentgrass Growth. Through the creeping bentgrass in four different periods of chlorophyll content, MDA and soluble sugar content determination. This test result showed:With the MCPA concentration increases, creeping bentgrass decline of chlorophyll content in the same period of growth, MDA and soluble sugar content increased. MCPA used after the early pair of creeping bentgrass growth is large, to put off with MCPA handle time, to affect to creeping bentgrass growth is gradually decreased.

Effect of Multiple Gas Media Package on Preservation of Fresh Sweet Corn at Room Temperature

2013 ◽  
Vol 477-478 ◽  
pp. 1354-1358
Author(s):  
Jian Qing Meng ◽  
Xiao Yu Jia ◽  
Wei Qiao Yang ◽  
Xi Hong Li
Keyword(s):  
High Temperature ◽  
Water Content ◽  
Room Temperature ◽  
Sweet Corn ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Plate Count ◽  
Aerobic Plate Count

Effects of different methods of gas package on freshness of harvested fresh Sweet Corn, at room temperature in its shelf life were investigated. The harvested fresh Sweet Corn were packaged under vacuum, air, N2 or CO2, respectively, and then stored 90 days at 30°Cafter the high temperature sterilization. After the sterilization and during storage, the changes of aerobic plate count, water content, soluble sugar content, and TPA were determined. The results showed that compared with others, the package with N2 could inhibit the growth of microorganisms, kept the content of water, soluble sugar and fat. After 90 days, TPA showed that the package in nitrogen could improve the quality of sweet corns.

scholarly journals The Effect of Low-dose X-ray Irradiation on the Quality of Fresh-cut Asparagus in Microwaveable Vacuum Skin Packs

HortScience ◽  
2011 ◽  
Vol 46 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Joongmin Shin ◽  
Bruce Harte ◽  
Janice Harte ◽  
Kirk Dolan
Keyword(s):  
Low Dose ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Water Soluble ◽  
Asparagus Officinalis ◽  
Local Source ◽  
X Ray ◽  
Pal Activity ◽  
Irradiation Treatment ◽  
Fresh Cut

Low-dose x-ray irradiation was used on vacuum skin-packaged fresh-cut asparagus to extend its shelf life and improve its microbial safety. Freshly imported Peruvian asparagus (Asparagus officinalis L.) spears were obtained from a local source, cleaned, trimmed, and sanitized and then vacuum skin-packaged (VSP) in a tray pack system. A breathable lid film was vacuum-sealed to protect the product from contamination and allow for gas exchange. Asparagus were packaged in VSP, which modified the package atmosphere, and exposed to a target dose of 1.0 kGy or left untreated. Asparagus were also placed in low-density polyethylene (LDPE) bags as a control. All samples were then stored at 4 °C for 24 days. To determine the effectiveness of the irradiation treatment, sample packages were periodically removed from storage and several measurements were used to evaluate the product, including headspace gas content, microbial growth, water soluble sugar content, and enzyme activity. A dose of 1.0 kGy reduced initial microbial population up to 3.8 log colony-forming units (cfu)/g and maintained 20% more of the initial sugar content relative to the non-irradiated VSP asparagus. Phenylalanine ammonia-lyase (PAL) activity in VSP asparagus was lower than in the control package (LDPE bag) regardless of irradiation treatment (P < 0.05). However, x-ray treatment increased the PAL activity of asparagus in VSP up to Day 8 (P < 0.05).

scholarly journals Effects of Photoperiod Interacted with Nutrient Solution Concentration on Nutritional Quality and Antioxidant and Mineral Content in Lettuce

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 920 ◽  
Author(s):  
Jiali Song ◽  
Hui Huang ◽  
Shiwei Song ◽  
Yiting Zhang ◽  
Wei Su ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Nutritional Quality ◽  
Mineral Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Photosynthetic Pigment ◽  
Solution Concentration ◽  
Plant Weight ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

The interacted effects of photoperiod and nutrient solution concentrations (NSCs) on nutritional quality and antioxidant and mineral content in lettuce were investigated in this study. There were a total of nine treatments by three photoperiods (12 h/12 h, 15 h/9 h, and 18 h/6 h), with a combination of three NSCs (1/4, 1/2, and 3/4 NSC). The contents of photosynthetic pigment, mineral element, and nutritional quality were markedly affected by the combination of photoperiod and NSC. The highest leaf number and plant weight were found in lettuce under the combination of 18–0.25X. There was a higher content of photosynthetic pigment in treatment of 15-0.25X. Shorter photoperiod (12 h/12 h and 15 h/9 h) and NSC (1/4 and 1/2 NSC) contributed to reduced nitrate contents and higher contents of free amino acid, soluble protein, and vitamin C. Longer photoperiod and lower NSC could increase soluble sugar content. The content of total P, K, and Ca exhibited a similar trend under the combination of photoperiod and NSC, with a higher content at 3/4 NSC under different photoperiods. Lower contents of total Zn and N were found under longer photoperiod. Moreover, higher antioxidant contents, including 2, 2-diphenyl-1-picrylhydrazyl (DPPH), value of ferric-reducing antioxidant power (FRAP), flavonoid, polyphenol, and anthocyanin were observed under shorter photoperiod, with the peak under 12-0.50X. Generally, 12-0.50X might be the optimal treatment for the improvement of the nutritional quality of lettuce in a plant factory that produced high-quality vegetables.

Phylogenetic Relationships of Sucrose Transporters (SUTs) in Plants and Genome-wide Characterization of SUT Genes in Orchidaceae

2020 ◽  
Author(s):  
Yunzhu Wang ◽  
Yue Chen ◽  
Qingzhen Wei ◽  
Hongjian Wan ◽  
Chongbo Sun
Keyword(s):  
Plant Species ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Total Content ◽  
Water Soluble ◽  
Long Distance ◽  
Sucrose Transporters ◽  
Specific Subgroup ◽  
Plant Level ◽  
Sucrose Partitioning

Abstract BackgroundSucrose is the primary form of photosynthetically produced carbohydrates transported long distance in many plant species, which significantly affects plant growth, development and physiology. Sucrose transporters (SUTs or SUCs) are a group of membrane proteins that play vital roles in mediating sucrose allocation within cells and at the whole plant level.ResultsIn this study, we investigated the relationship of SUTs in 24 representative plant species and performed a comprehensive analysis of SUT genes in three sequenced Orchidaceae species, Dendrobium officinale, Phalaenopsis equestris, and Apostasia shenzhenica. All the SUTs from 24 plants were classified into three groups and five subgroups: subgroups A, B1, B2.1, B2.2, and C, based on the evolutionary relationships. A total of 22 SUT genes were identified in Orchidaceae species, among which D. officinale had 8 genes (DenSUT01-08), P. equestris had 8 genes (PeqSUT01-08) and A. shenzhenica had 6 genes (PeqSUT01-06). For the 22 Orchidaceae SUTs, each of the subgroups A, B2.2 and C contains three genes, whereas the SUT genes were significantly expanded in the monocot-specific subgroup B2.1 which contained 12 genes. To shed light into sucrose partitioning and functions of sucrose transporters in Orchidacea species, we analysed water-soluble sugar content and performed RNA sequencing of different tissues of D. officinale, including leaves, stems, flowers and roots. The results showed that although total content of water-soluble polysaccharides was highest in the stems of D. officinale, the sucrose content was highest in flowers. Moreover, gene expression analysis showed that most of the DenSUTs were expressed in flowers, among which DenSUT01, DenSUT07 and DenSUT06 had significantly high expression levels.ConclusionsThese results indicated that stems are used as main storage sinks for photosynthetically produced sugar in D. officinale, and that the DenSUTs mainly take functions in the cellular machinery and development of floral organs. Our findings provide valuable information on sucrose partitioning and the evolution and functions of SUT genes in Orchidaceae and other species.

scholarly journals Effects of sucrose and irradiance on germination and early gametophyte growth of the endangered tree fern Dicksonia sellowiana Hook (Dicksoniaceae)

2004 ◽  
Vol 18 (2) ◽  
pp. 375-380 ◽  
Author(s):  
Gladys Daniela Rogge Renner ◽  
Áurea Maria Randi
Keyword(s):  
Chlorophyll Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Dry Mass ◽  
Ms Medium ◽  
Tree Fern ◽  
Endangered Tree ◽  
Germination Time ◽  
Mean Germination Time ◽  
The Tropics

It is an endangered tree fern that grows in mixed umbrophylus forests in the tropics. Sterilized spores were germinated at 25 ± 2ºC under a 16-hour photoperiod, in Dyer and MS medium supplemented with 0 to 5% sucrose. The germination was lower with the addition of sucrose. Dry mass was higher in 30-day-old gametophytes cultivated in Dyer medium with the addition of 3 to 5% of sucrose. The dry mass decreased in 30-day-old gametophytes cultivated in Dyer and MS media without sucrose and in MS medium with the addition of 4 or 5% of sucrose. The effect of different irradiance on the germination and early gametophyte development of D. sellowiana was analyzed in the autumn of 1998 (May until July). Conical flasks containing spores were kept over a period of 49 days in 50cm³ boxes covered with black shade netting, which gave 5, 20, 36 and 50% of irradiance. The lower mean germination time was observed for spores cultivated under 5% and 20% of irradiance. The highest chlorophyll content was recorded in gametophytes cultivated for 49 days under 20% and 5% of irradiance .The highest soluble sugar content was recorded in gametophytes cultivated under 5% and 20% of irradiance.

scholarly journals Comparative Effects of Salt and Alkali Stress on Antioxidant System in Cotton (Gossypium Hirsutum L.) Leaves

2019 ◽  
Vol 17 (1) ◽  
pp. 1352-1360 ◽  
Author(s):  
Huijuan Guo ◽  
Zhiqiang Hu ◽  
Huimin Zhang ◽  
Wei Min ◽  
Zhenan Hou
Keyword(s):  
Chlorophyll Content ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Seedling Emergence ◽  
Nacl Stress ◽  
Alkali Stress ◽  
Salt Tolerant ◽  
Sod Activity ◽  
Physiological And Biochemical Characteristics ◽  
Mda Content

AbstractThis pot experiment was to evaluate how salts (NaCl, Na2SO4) and alkali (Na2CO3+NaHCO3) affect the physiological and biochemical characteristics during the seedling stage of two cotton cultivars (salt-tolerant, L24; salt-sensitive, X45). Salt and alkali stress reduced seedling emergence rate, relative biomass, and chlorophyll content, however, the REC and MDA content increased. Salt and alkali stress increased markedly superoxide dismutase (SOD) activity. Peroxidase (POD) activity increased first and then decreased as the increase of salt and alkali stress. Catalase (CAT) activity initially increased and then decreased as NaCl stress increased. In addition, the SOD activity, REC, and MDA content was markedly higher in salt stress than that in alkali stress. The proline content of L24 was higher than that of X45 under salt and alkali stress. However, glycine betaine and soluble sugar content of L24 was lower than that of X45 under alkali stress. The REC and MDA content of L24 were lower than those of X45, however, the relative biomass, chlorophyll content, SOD, POD, CAT, and Pro were higher than those of X45. In conclusion, salt tolerant cotton cultivars may possess a superior protection effect by increasing antioxidant enzymes activity under salt and alkali stress.

Effects of Different Light Intensity on the Growth and Nutrients Content of Gynura

2014 ◽  
Vol 955-959 ◽  
pp. 3766-3769 ◽  
Author(s):  
Xiang Rong Cheng ◽  
Jia Liu ◽  
Jun Shu ◽  
Mu Kui Yu
Keyword(s):  
Light Intensity ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Light Condition ◽  
Amino Acid Content ◽  
Canopy Cover ◽  
Water Soluble ◽  
Light Conditions ◽  
Significant Difference ◽  
Number Of Branches

Light condition has significant effects on plant growth and nutrient accumulation. The growth and nutrients content of Gynura (Begonia fimbristipulata) were studied under three light intensities (natural field, 100% full sunlight (L0); 50% canopy cover (L1); 70% canopy cover (L2)) in Fuyang, China. The results showed that plant height, ground diameter and number of branches were no significant difference between L0 and L1 treatments (P>0.05), these variables were significantly lower in L2 treatment than that in L0 treatment (P<0.05). Potassium (K), phosphorus (P), vitamin C (Vc) and soluble sugar content in Gynura’s leaves reduced with decreasing light intensity. The content of protein and iron (Fe) were no significant difference among the three light treatments (P>0.05). The content of calcium (Ca) markedly increased in L1 treatment compared with L0 treatment (P<0.05), and it greatly declined in L2 treatment. The change of light conditions had less impact on the water-soluble amino acid content (except for alanine, serine, and glutamic acid). These results indicate that Gynura is suitable to cultivate under the forest with 50% canopy cover.

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