Echogenicity and stiffness coefficient of the liver tissue in comparison with similar indicators of the renal parenchyma as an early manifestation of metabolic syndrome

Non-alcoholic fatty liver disease (NAFLD) merupakan penyakit perlemakan pada hati yang terjadi pada penderita sindrom metabolik. Penderita sindrom metabolik terjadi peningkatan kadar stres oksidatif sehingga muncul sel steatosis dan pelebaran sinusoid hati. Senyawa flavonoid dalam Zingiber officinale (jahe gajah) diketahui memiliki efek hepatoprotektif dan antiinflamasi dengan cara menghambat pembentukan reactive oxygen species (ROS). Tujuan penelitian ini mengetahui pengaruh fraksi etil asetat jahe gajah terhadap mikrostruktur jaringan hati pada mencit model sindrom metabolik. Objek penelitian ini menggunakan mencit jantan galur Swiss Webster yang berusia 36−40 minggu dibagi menjadi 4 kelompok. Penelitian dilakukan di Laboratorium Farmasi Institut Teknologi Bandung. Kelompok kontrol yang diberi pakan tinggi lemak tanpa diberikan terapi selama 28 hari. Kelompok II−IV diberi pakan tinggi lemak dan diterapi dengan diberi fraksi etil asetat jahe gajah dengan konsentrasi 0,78 mg, 1,56 mg, dan 3,12 mg per kilogram bobot per hari diberikan secara oral. Observasi dan kuantifikasi mikrostruktur jaringan hati dilakukan menggunakan mikroskop cahaya. Hasil statistik jumlah sel steatosis belum menunjukkan hasil yang signifikan (p>0,05), sedangkan pada jumlah pelebaran sinusoid menunjukkan hasil yang signifikan (p<0,05). Kekuatan korelasi konsentrasi fraksi jahe gajah dengan jumlah sel steatosis rendah, tetapi pasti (r=-0,381) dan pelebaran sinusoid cukup berarti (r=-0,451). Simpulan penelitian ini adalah pemberian fraksi etil asetat jahe gajah memengaruhi mikrostruktur jaringan hati pada mencit model sindrom metabolik. LIVER TISSUE MORPHOLOGICAL CHANGES BY ZINGIBER OFFICINALE FRACTIONS IN METABOLIC SYNDROME MICE MODELSNon-alcoholic fatty liver disease (NAFLD) is a fatty liver disease that occurs in patients with metabolic syndrome. Patients with metabolic syndrome occur closer to oxidative stress that occurs in steatosis and dilation of the liver sinusoid. Flavonoid compounds in Zingiber officinale have hepatoprotective and anti inflammatory effects by inhibiting the formation of reactive oxygen species (ROS). The purpose of this study was to determine the content of Zingiber officinale ethyl acetate fraction on liver tissue microstructure in mice model of metabolic syndrome. This research method using mice of Swiss webster strain which had 36−40 weeks, divided into 4 groups. The study conducted at Pharmacology Laboratory Institut Teknologi Bandung. Control group fed high fat without therapy for 28 days. Group II−IV were fed high fat and treated with ginger elephant ethyl acetate fraction with a concentration of 0.78 mg, 1.56 mg and 3.12 mg per kilograms of body weight per day, given orally. Observation and quantification of liver tissue microstructure was performed using a light microscope. The statistical results on steatosis cell counts did not show significant results (p>0.05), whereas the number of sinusoid enlargement showed significant results (p<0.05). Alternative strength of the Zingiber officinale fraction with a low but definite steatosis cell number (r=−0.381) and significant sinusoid widening (r=−0.451). In conclusion, that administration of ginger elephant ethyl acetate fraction affected microstructure of liver tissue in mice model of metabolic syndrome.

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0253: Sympathetic overactivity: a very early manifestation of metabolic syndrome

Archives of Cardiovascular Diseases Supplements ◽

10.1016/s1878-6480(14)71291-1 ◽

2014 ◽

Vol 6 ◽

pp. 11

Author(s):

Safir Habib ◽

Philippe Van De Borne ◽

Nathalie Niederhoffer ◽

Erik Sauleau ◽

Eliane Albuisson ◽

...

Keyword(s):

Metabolic Syndrome ◽

Sympathetic Overactivity ◽

Early Manifestation

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Synbiotic Goat Milk Kefir Lowered Peroxisome Proliferator Activated Receptor Gamma (PPARγ) Gene Expression in Rat Adipose and Liver Tissue

10.21203/rs.3.rs-159681/v1 ◽

2021 ◽

Author(s):

Nurliyani Nurliyani ◽

Eni Harmayani ◽

Sunarti Sunarti

Keyword(s):

Gene Expression ◽

Metabolic Syndrome ◽

Blood Glucose ◽

Liver Tissue ◽

Goat Milk ◽

Peroxisome Proliferator ◽

Milk Product ◽

High Fat ◽

Peroxisome Proliferator Activated Receptor ◽

Insulin Producing Cells

Abstract Background Kefir is a fermented milk product containing bacteria and yeast, whereas glucomannan from porang (Amorphophallus oncophyllus) tuber is known as a prebiotic in vivo. Diets with a high fat and high sugar will stimulate metabolic syndrome associated with changes in gene expression including peroxisome proliferator activated receptor gamma (PPARγ). The purpose of this study was to determine the effect of goat milk kefir enriched with porang glucomannan (synbiotic kefir) and goat milk kefir without glucomannan (probiotic kefir) on blood glucose, hemoglobin A1c (HbA1c), free fatty acid (FFA), tumor necrosis factor alpha (TNF-α), gene expression of peroxisome proliferator activated receptor gamma (PPARγ), and insulin-producing cells in rats fed a high-fat and high-fructose (HFHF) diet. Methods Male Sprague Dawley rats 8–12 weeks old (n = 30) treated with HFHF diets for two weeks, and then divided into five dietary groups (each group consisted of 6 rats): 1) normal control (received a standard diet only); 2) rats fed HFHF; 3) rats fed HFHF + probiotic kefir; 4) rats fed HFHF + synbiotic kefir; and 5) rats fed HFHF + simvastatin. The dose of kefir was 3.6 mL/200 g body weight/day and simvastatin was 0.72 mg/day. All of these treatments were carried out for 4 weeks. Results There were no significant differences in plasma blood glucose in HFHF rats after and before treatment, but decreased in plasma HbA1c and TNFα (p < 0.05) and inhibited the increase of FFA in rats after synbiotic kefir treatment (paired-samples t-test). Probiotic and synbiotic kefir decreased the gene expression of PPARγ2 (p < 0.05) in both of adipose and liver tissue in HFHF rats but had no effect on the total number of Langerhans islets and insulin-producing cells (one way ANOVA). Conclusions Synbiotic kefir could ameliorate the health of rats fed HFHF diet through decreasing HbA1c, TNFα, and PPARγ2 gene expression and preventing an increase in FFA. The results indicate that goat milk synbiotic kefir potentially improve metabolic syndrome.

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NAFLD progression is related with plasma pro-oxidative biomarkers rather than liver tissue-measured nitrogen metabolism biomarkers in population with obesity and metabolic syndrome

10.21203/rs.2.21311/v1 ◽

2020 ◽

Author(s):

Juan Antonio Suárez-Cuenca ◽

Gabriela Alexandra Domínguez-Pérez ◽

Rolando Efraín Hernández-Muñóz ◽

Alejandro Hernández-Patricio ◽

Eduardo Vera-Gómez ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Nitrogen Metabolism ◽

Liver Tissue ◽

Cardiometabolic Risk ◽

Metabolic Phenotype ◽

Alcoholic Fatty Liver ◽

The Metabolic Syndrome ◽

Study Population ◽

And Oxidative Stress

Abstract Background. The metabolic syndrome (MS) is associated with an increased production of nitrogen metabolites and elevated oxidative stress, which favor the development and progression of non-alcoholic fatty liver disease (NAFLD). Subjects with the phenotype known as metabolically unhealthy obese (MUO) meet most of the MS cardiometabolic risk criteria and show a higher prevalence of NAFLD and fibrosis progression risk, compared to the so-known as metabolically healthy obese (MHO). The present study aimed to explore whether liver-specific biomarkers of nitrogen metabolism and oxidative stress, measured in both plasma and in liver tissue, may relate to NAFLD severity and/or metabolic phenotype. Methods. This observational, cross-sectional study included candidates for bariatric surgery with biopsy-proven NAFLD diagnosis and staging. For comparison, the study population was divided according to NAFLD progression (steatohepatitis F0-F1 vs F2-F4) and metabolic phenotype (MHO vs MUO, based on the MS criteria). Hepatic and plasma concentrations of nitrogen metabolites and oxidative stress biomarkers were determined by enzymatic kinetics assays, ELISA and Griess reaction. Results. The study population consisted of 28 patients with obesity and higher prevalence of dyslipidemia, diabetes mellitus and hypertension. NAFLD progression was related with the metabolic phenotype. According to plasma biomarkers, MUO phenotype was related to higher cardiometabolic risk; while NAFLD severity progression was related to higher Hb A1c and triglycerides. Elevated hepatic concentrations of ammonium, nitrites, arginine and citrulline were found in MUO phenotype, but only higher plasma concentration of MDA was found as specifically related to NAFLD progression. Conclusions. Circulating biomarkers of redox state were selectively related with NAFLD progression, supporting prognostic and therapeutic potential target. Hepatic concentrations of nitrogen metabolism biomarkers may be more related to cardiometabolic risk.

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PECULIARITIES OF STRUCTURAL-FUNCTIONAL STATE OF THE KIDNEYS ACCORDING ELASTOGRAPHY SHEARWAVE IN CHILDREN WITH ARTERIAL HYPERTENSION ON THE BACKGROUND OF OBESITY

Clinical Practice ◽

10.17816/clinpract738-15 ◽

2016 ◽

Vol 7 (3) ◽

pp. 8-15

Author(s):

A V Borsukov ◽

V V Bekezin ◽

E U Kozlova ◽

V S Zuy

Keyword(s):

Metabolic Syndrome ◽

Arterial Hypertension ◽

Shear Wave ◽

Functional State ◽

Shear Wave Elastography ◽

Renal Parenchyma ◽

Control Group ◽

Noninvasive Method ◽

Cardiovascular Morbidity And Mortality ◽

Method Of Evaluation

Introduction. Metabolic and hemodynamic consequences of obesity (insulin resistance and hyperglycemia, hypertension, dyslipidemia) rofm metabolic syndrome in adolescence and an accordingly high risk of cardiovascular morbidity and mortality in adulthood. In recent years, studies have shown that obesity and metabolic syndrome are independent risk factors for chronic kidney disease (CKD). Ultrasonography is the most commonly used noninvasive method for research of the kidneys in pediatric patients.Aim of the study: to assess new features of shear wave elastography for the diagnosis of structural-functional state of the kidneys in children with arterial hypertension and obesity. Materials and methods. Ultrasound examination of kidneys was performed using a digital portable ultrasound system ANGIODIN - sono / P - Ultra (NPF “BIOS” Russia). Was examined 83 children who underwent elastography shear wave parenchymal layer of the kidney definition of renal parenchymal stiffness in kPa.Results. In patients with arterial hypertension and obesity the results of elastography, shear waves we found significant differences in the elasticity of the renal parenchyma between those with arterial hypertension (group 1, n=37) and children with uncomplicated obesity (group 2, n=26). The elasticity of the renal parenchyma in patients of the control group (n=20) was significantly lower than in patients of the 1st and 2nd groups. The results show that in children of the 1st group with stable hypertension high values of the elasticity of the renal parenchyma were detected in 3,2 times more often in comparison with children of the 1st group were diagnosed with labile hypertension. Conclusion. The shear wave elastography is an informative noninvasive method of evaluation of the kidneys in children with arterial hypertension with obesity. This diagnostic approach can be used in pediatric patients as a screening method (before ABPM) to assess the risk of developing hypertension, degree of hypertension and the adequacy of current anti-hypertensive therapy.

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