Assessment of Cytotoxic and Antioxidant Effects of Rosmarinus officinalis and Fluoride containing Dentifrice - An In vitro Study

2021 ◽  
pp. 3779-3784
Author(s):  
Sushanthi S. ◽  
Jayashri Prabakar ◽  
Meignana Arumugham Indiran ◽  
R. Pradeep Kumar ◽  
Srisakthi Doraikannan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Rosmarinus Officinalis ◽  
Control Measures ◽  
Oral Diseases ◽  
Brine Shrimp Lethality Assay ◽  
Antioxidant Effects

Background: Rosmarinus officinalis (rosemary) is a medicinal plant native to the Mediterranean region and cultivated around the world. This medicinal plant possesses antimicrobial and antioxidant properties. It has been proved that naturally occurring active ingredients of herbal medicinal plants shows least adverse effects and can act as effective oral hygiene aid for the control of common oral diseases without the need for synthetically derived or chemical agents. Hence, the present study invitro was designed to assess the cytotoxic and antioxidant effects of rosemary so that it can be used as an effective plaque control measures against various oral diseases. Materials and Methods: Rosemary leaves were procured from the markets of South India and were powdered to prepare the aqueous and ethanolic plant extract. The plant extracts were then subjected for cytotoxic and antioxidant analysis. Cytotoxic effects were evaluated using Brine shrimp lethality assay and antioxidant effects using DPPH radical scavenging assay. After the evaluation of antioxidant and cytotoxic property, rosemary-based toothpaste using herbal tooth formulation was prepared. Results: The results show that 5,10 and 15µl conc. all the nauplii fishes were alive in rosemary toothpaste whereas 60% of fishes were dead. Rosemary toothpaste have good antioxidant property when compared to fluoride toothpaste. Descriptive statistics were used. Conclusion: From the above findings, rosemary containing dentifrice has very less cytotoxicity and good antioxidant properties when compared with fluoride dentifrice and it can be served as an excellent replacement of several commercially available dentifrices.

Antigenotoxic and antioxidant effects of the Mongolian medicinal plant Leptopyrum fumarioides (L): An in vitro study

2014 ◽  
Vol 155 (1) ◽  
pp. 599-606 ◽  
Author(s):  
Delgerbat Boldbaatar ◽  
Hesham R. El-Seedi ◽  
Meriana Findakly ◽  
Safa Jabri ◽  
Batkhuu Javzan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antioxidant Effects

scholarly journals PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIOXIDANT STATUS OF CLERODENDRUM VOLUBILE (AN INDIGENOUS MEDICINAL PLANT)

2016 ◽  
Vol 2 (2) ◽  
pp. 77 ◽  
Author(s):  
Tomisin Happy Ogunwa ◽  
Tolulope Tosin Adeyelu ◽  
Rotimi Yemi Fasimoye ◽  
Mary Bose Oyewale ◽  
Taiwo Ademola Ademoye ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Research Work ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Natural Antioxidants ◽  
Therapeutic Effects ◽  
Dpph Radical

ABSTRACTClerodenrum volubile is an important locally used medicinal plant. It is one of the essential herbs nature has provided for mankind to be consumed as spices, vegetable and also used in the treatment of diseases. Qualitative phytoconstituent screening of the plant revealed the presence of steroids, flavonoids, tannins, saponins, and phenolic compound while chalcones, alkaloid and anthraquinone were absent. Quantitative phytochemical evaluation showed values of 8.29±1.26mg/g rutin equivalent for flavonoids, 3.53±0.05mg/g gallic acid equivalent for phenol, 3.97±0.03mg/g tannic acid equivalent for tannins and 13.67±1.27% for saponin per dry weight. As it is imperative to extend research work on therapeutic effects of the arsenal of plants nature has given to us in Africa so as to obtain a cure for various diseases attacking human’s health, antioxidant properties of the plant was evaluated. Antioxidant models used include iron chelating, DPPH radical, superoxide ion, hydrogen peroxide, ABTS radical, hydroxyl radical scavenging activities and ferric ion reducing properties. The obtained IC50 values against DPPH radical were 141.342 and 120.349µg/mL for Clerodendrum volubile and trolox respectively. The chelating effect of the plant extract at 50% inhibition was close to that of ascorbic acid (standard) with 134.34 and 131.19µg/mL concentration respectively. Overall, the aqueous extract of the plant showed antioxidant potential which was close to the effects exerted by known standard antioxidants (ascorbic acid, trolox and EDTA). The plant could hence provide natural antioxidants which are needed to combat numerous free radical-mediated diseases and complications such as aging, cancer, atherosclerosis, which are linked with oxidative stress. 

scholarly journals A COMPARATIVE STUDY OF IN-VITRO NITRIC OXIDE SCAVENGING ACTIVITY OF BALOFLOXACINVS PRULIFLOXACIN

2016 ◽  
Vol 10 (1) ◽  
pp. 380
Author(s):  
Puja Ranka ◽  
Karthik Vp
Keyword(s):  
Nitric Oxide ◽  
Sodium Nitroprusside ◽  
Room Temperature ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
In Vitro Study ◽  
Scavenging Activity ◽  
Acid Reagent

ABSTRACTObjectives: To compare the free radical scavenging activity and antioxidant potentials of balofloxacin versus prulifloxacin.Methods: Nitric oxide (NO) radical scavenging activity was determined using the method of Garret (1964). Sodium nitroprusside in aqueous solutionat physiological pH spontaneously generates NO, which interacts with oxygen to produce nitrite ions which can be determined by the use of Griessilosvay reaction. 2 mm of 10 mm sodium nitroprusside in 0.5 ml phosphate buffer saline (pH 7.4) was mixed with 0.5 ml prulifloxacin and balofloxacinat various concentrations and the mixture incubated at 25°C for 150 minutes. From the incubated mixture 0.5 ml was taken out and added into 1.0 mlsulfanilic acid reagent (30 in 20% glacial acetic acid) and incubated at room temperature for 5 minutes. Finally, 1.0 ml naphthylethylenediaminedihydrochloride (0.1% w/v) was mixed and incubated at room temperature for 30 minutes. The resultant absorbance was recorded at 540 nm using a spectrometer. The percentage inhibition was calculated using the formula:−Abs AbsPercentage inhibition 100control sample= ×AbsResults: At concentration of 10, 20, 30, and 40 µg/ml percentage inhibition observed with prulifloxacin and balofloxacin was 40.94, 53.09, 66.25, 88.06 and 34.49, 49.09, 60.42, and 73.14, respectively. controlConclusion: Thus, this in vitro study suggests that both prulifloxacin and balofloxacin possess antioxidant properties. At the lower doses both thedrugs show similar antioxidant profile, while at the higher doses the antioxidant potential of prulifloxacin is significantly high than balofloxacin.However, the preference of selecting one over the other depends on the indication as well as the risk-benefit ratio.Keywords: In vitro, Nitric oxide, Prulifloxacin, Balofloxacin.

Evaluation of Safety, Antileishmanial, and Chemistry of Ethanolic Leaves Extracts of Seven Medicinal Plants: An In-vitro Study

2020 ◽  
Vol 7 (1) ◽  
pp. 26-36
Author(s):  
Nargis Shaheen ◽  
Naveeda Akhter Qureshi ◽  
Attiya Iqbal ◽  
Asma Ashraf ◽  
Huma Fatima
Keyword(s):  
Plant Extracts ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chemical Characterization ◽  
In Vitro Study ◽  
Toxicity Assessment ◽  
Antileishmanial Activity ◽  
Total Phenolic ◽  
Brine Shrimp Lethality Assay

Background: Cutaneous leishmaniasis is a neglected tropical disease that currently affects people among 98 countries and causes significant morbidity and mortality. Current chemotherapeutic intervention is unsatisfactory and has various limitations that highlight the necessity to develop safe and effective therapeutic approaches from natural products. Objective: The main objective of current study was the evaluation of the antileishmanial activity along with toxicity assessment of selected plant extracts. Methods: The ethanolic leaves extracts of selected plants were evaluated for their qualitative and quantitative phytochemical screening by standard protocols. The antioxidant potential of plant extracts was determined by total antioxidant capacity, ferric reducing power and DPPH radical scavenging assays. The cytotoxicity analysis using brine shrimp lethality assay and in-vitro antileishmanial activity against promastigotes of L. tropica (Accession# MN891719) were also evaluated. Results: The preliminary examination of crude extracts revealed that P. armeniaca showed the highest total phenolic and flavonoid content (279.62±5.40µgGAE/mgDW and 205.70 ±2.41µgQA/mgDW, respectively), among others. P. armeniaca showed strongest antioxidants (120.37±4.90 µgAAE/mgDW) and FRP values (278.71±1.03µgAAE/mgDW). All the plant extracts showed cytotoxicity in safety range >1000µg/ml except F. glomerata having LC50 values of 454.34 µg/ml. In the present study, P. communis and P. pashia showed some level of activity (LC50 56.68 and 60.95µg/ml respectively) while P. armeniaca demonstrated the highest antileishmanial activity (LC50 16.18µg/ml). Conclusion: The findings are highly encouraging so, further and extensive investigations of P. arminica should be carried out; especially bio guided fractionation to identify the active fraction and further chemical characterization of structure.

Extraction, Chemical Composition, Antioxidant Property and In vitro Anticancer Activity of Silymarin from Silybum Marianum On Kb and A549 Cell Lines

2020 ◽  
Vol 17 ◽  
Author(s):  
Mojgan Azadpour ◽  
Mohammad Mehdi Farajollahi ◽  
Ali Mohammad Varzi ◽  
Pejman Hashemzadeh ◽  
Hossein Mahmoudvand ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
A549 Cell ◽  
Hplc Analysis ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Silybum Marianum ◽  
Stable Free Radical

Introduction: This study aimed to evaluate the antioxidant property of silymarin (SM) extracted from the seed of Silybum marianum and its anticancer activity on KB and A549 cell lines following 24, 48, and 72 h of treatment. Methods: Ten grams of powdered S. marianum seeds were defatted using n-hexane for 6 hours and then extracted by methanol. The silymarin extracted of extraction components The extracted components of silymarin were measured by spectrophotometric assay and HPLC analysis. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, phenol content, total flavonoid content, and total antioxidant capacity were measured to detect the antioxidant properties of SM. The anticancer activity of the SM on cell lines evaluated by MTT. Results: In HPLC analysis, more than 50% of the peaks were related to silibin A and B. SM was reducedDPPH (the stable free radical) with a 50% inhibitory concentration (IC50) of 6.56 μg/ ml in comparison with butylated hydroxyl toluene (BHT), which indicated an IC50 of ~3.9 μg/ ml.The cytotoxicity effect of SM on the cell lines was studied by MTT assay. The cytotoxicity effect of the extracted silymarin on KB and A549 cell lines was observed up to 80 and 70% at 156 and 78 µg/ml, respectively. The IC50 value of the extracted SM on KB and A549 cell lines after 24 hours of treatment was seen at 555 and 511 µg/ml, respectively. Conclusion: Due to the good antioxidant and anticancer properties of the isolated silymarin, its use as an anticancer drug is suggested.

scholarly journals Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1081
Author(s):  
Matilda Rădulescu ◽  
Călin Jianu ◽  
Alexandra Teodora Lukinich-Gruia ◽  
Marius Mioc ◽  
Alexandra Mioc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
In Silico ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Melissa Officinalis ◽  
Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

scholarly journals IN VITRO ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITIES OF HINGULESWARA RASABASED HERBOMINERAL FORMULATIONS

2018 ◽  
Vol 11 (14) ◽  
pp. 24
Author(s):  
Abhishek Chatterjee ◽  
Dileep Singh Baghel ◽  
Bimlesh Kumar ◽  
Saurabh Singh ◽  
Narendra Kumar Pandey ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Standard Drug ◽  
Three Samples ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Antioxidant Effects ◽  
Made In ◽  
In Vitro Antioxidant Activity

Objective: The aims of the present investigation were to develop the herbal and/or herbomineral formulations of Hinguleswara rasa and to compare their anti-inflammatory and antioxidant activities, in vitro, with that of standard drug samples.Methods: This study was an interventional investigation in three samples: In the first sample, Hinguleswara rasa (HR1) was prepared as per methodology described in Rasatarangini using Shuddha Hingula (10 g), Shuddha Vatsanabha (10 g), and Pippali (10 g). In the second and third sample, respectively, Hinguleswara rasa was prepared by replacing Shuddha Hingula with Kajjali where Kajjali made from Hingulotha parada and Sodhita parada constitutes two varieties of Hinguleswara rasa, i.e. HR2 and HR3. In vitro antioxidant activity was studied using 2,2-diphenyl-1-picrylhydrazyl, and the absorbance was recorded at 517 nm. For evaluating the in vitro anti-inflammatory studies, the inhibition of albumin denaturation technique was performed.Results: The results showed that the formulation of Hinguleswara rasa has shown dose-dependent activity which was observed in 100 μg concentration. HR1, HR2, and HR3 showed 36.11, 17.22, and 16.11% radical scavenging activity.Conclusion: It could be concluded that the changes made in the formulations did not affect the in vitro anti-inflammatory and antioxidant effects of the herbomineral formulations.

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