Effect of Boiling on Carotenoids content of Pumpkin available in Damascus, Syria

2021 ◽  
pp. 5642-5646
Author(s):  
Hala Juha ◽  
Fida Amali
Keyword(s):  
Linear Regression ◽  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Beta Carotene ◽  
Local Market ◽  
Beer's Law ◽  
Beer’S Law ◽  
Standard Solution ◽  
Regression Correlation

The carotenoids contents of pumpkin were determined in this study by spectrophotometric method. This method was applied on beta-carotene as standard solution as well as samples. The absorption were determined at λmax 454 nm, the analytical method was validated by evaluating linearity, precision, accuracy, LODs, and LOQs of the analyts. Beer’s law is obeyed over the concentration of (0.3-9) µg/ml with a linear regression correlation coefficient of 0.9986, and recovery in range from 98.5-102.2%. After the validation was performed using standard solution, the method then was applied on samples of pumpkin available in local market in Syria after extraction the carotenoids by using Acetone/ Dichloromethane 70/30 .The results ranged between 216.46 - 80.56 µg/g. During boiling for pumpkin, the level of carotenoid reduced between 0.45-13.26 %, 0.18 – 23.07%, 17.3-40.54%, 22.65-51.73%, 14.91_55.82% and 26.16 – 72.56%, for 5, 10, 15, 30, 45 and 60 min respectively. According to the obtained result results, it is concluded that the boiling reduced carotenoid at different rates.

Development and Validation of UV Spectrophotometric Method For The Estimation of Finasteride Drug

2019 ◽  
Vol 10 (04) ◽  
pp. 597-600
Author(s):  
Divya Sanganabhatla ◽  
R Shyam Sunder
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Highly Sensitive ◽  
Beer's Law ◽  
Recovery Study ◽  
Beer’S Law ◽  
Development And Validation

A simple, accurate, precise, reproducible, highly sensitive, an economic spectrophotometric method has been developed for the estimation of finasteride. UV spectrophotometric method is based on the measurement of absorption at a maximum wavelength of 255 nm. The developed method was validated with respect to linearity, accuracy (recovery), Precision (inter and intraday variations), LOD and LOQ. Beer’s law was obeyed in the concentration range of 5 – 25 μg/mL with a correlation coefficient of 0.9933. Results of the analysis were validated statistically and by recovery study. Hence the developed and validated method can be used for estimation of finasteride.

scholarly journals UV Spectrophotometric Estimation of Acipimox inBulk and Capsule Dosage Form

2009 ◽  
Vol 6 (4) ◽  
pp. 1117-1120 ◽  
Author(s):  
A. Elphine Prabahar ◽  
B. Thangabalan ◽  
R. Kalaichelvi ◽  
P. Vijayaraj Kumar
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Ultra Violet ◽  
Molar Absorptivity ◽  
Distilled Water ◽  
Maximum Absorbance ◽  
Beer's Law ◽  
Beer’S Law

A new simple, rapid, accurate, sensitive and precise spectrophotometric method in ultra violet region has been developed for determination of acipimox (ACX) in bulk and capsule dosage form. Acipimox exhibited maximum absorbance at 231 nm with apparent molar absorptivity of 1.5104 × 104in distilled water. Beer’s law was found to be obeyed in the concentration range 1-10 μg mL-1. Correlation coefficient was found to be 0.9998. The developed method was validated respect to linearity, precision, accuracy. The proposed method is useful for the routine estimation of ACX in bulk and capsule dosage form.

VALIDATED SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF OLMESARTAN MEDOXOMIL IN BULK AND PHARMACEUTICAL TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (01) ◽  
pp. 33-39
Author(s):  
S. Y Rao ◽  
◽  
P. Gandi ◽  
V. P. K. Rao ◽  
H. T. Kumar
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Picric Acid ◽  
Olmesartan Medoxomil ◽  
Pharmaceutical Formulation ◽  
Spectrophotometric Methods ◽  
Beer's Law ◽  
Beer’S Law ◽  
Bulk Drug ◽  
Charge Transfer Complexation

Two simple, sensitive and rapid spectrophotometric methods have been developed for the estimation of Olmesartan medoxomil in bulk and pharmaceutical formulation. Method A is a UV spectrophotometric method in which isopropyl alcohol is used as solvent and exhibited absorption maxima at 285 nm. Beer’s law obeyed in the concentration range of 2 - 12 μg/mL with a correlation coefficient of 0.999. Method B is visible spectrophotometric method based on charge transfer complexation which involves the formation of a yellow colored chromogen when treated with picric acid. The chromogen exhibit absorption maxima at 421 nm and obeyed Beer’s law in concentration range 4-16 μg/mL with a correlation coefficient of 0.999. The results of the methods have been validated statistically and by recovery studies confirmed the accuracy and precision of the methods. The proposed methods are simple, sensitive and economical for quantitative determination of Olmesartan medoxomil in bulk drug and pharmaceutical formulation.

Novel UV Spectrophotometric Method for the Quantitative Analysis of CefpodoximeProxetil in Pharmaceutical Formulations by First Derivative Technique

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Potdar S. S. ◽  
Karajgi S. R. ◽  
Simpi C. C. ◽  
Kalyane N. V.
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
First Derivative ◽  
Good Linearity ◽  
Beer's Law ◽  
Beer’S Law ◽  
Tablet Dosage ◽  
Ich Guideline

The spectrophotometric method for estimation of CefpodoximeProxetil employed first derivative amplitude UV spectrophotometric method for analysis using methanol as solvent for the drug. CefpodoximeProxetil has absorbance maxima at 235nm and obeys Beer’s law in concentration range 10-50µg/ml with good linearity i.e. r2 about 0.999. The recovery studies established accuracy of the proposed method; result validated according to ICH guideline. Results were found satisfactory and reproducible. The method was successfully for evaluation of CefpodoximeProxetil in tablet dosage form without interference of common excipients.

scholarly journals A spectrophotometric method for determination of fluorophore-to-protein ratios in conjugates of the blue fluorophore 7-amino-4-methylcoumarin-3-acetic acid (AMCA)

1990 ◽  
Vol 38 (1) ◽  
pp. 87-94 ◽  
Author(s):  
M W Wessendorf ◽  
S J Tallaksen-Greene ◽  
R M Wohlhueter
Keyword(s):  
Acetic Acid ◽  
Optical Density ◽  
Extinction Coefficient ◽  
Spectrophotometric Method ◽  
Extinction Coefficients ◽  
Beer's Law ◽  
Beer’S Law ◽  
The Given

7-Amino-4-methylcoumarin-3-acetic acid (AMCA) has been found to be a useful fluorophore for immunofluorescence. The present study describes a spectrophotometric method for determining the ratio of moles AMCA to moles protein (or the f/p ratio) in an AMCA-conjugated IgG. The concentration of a substance absorbing light can be determined spectrophotometrically using Beer's Law: Absorbance = Concentration x Extinction coefficient. From Beer's law, one can derive the following formula for determining the f/p ratio of AMCA-IgG conjugates: f/p = (epsilon 280IgG).A350 - (epsilon 350IgG).A280/(epsilon 350AMCA).A280 - (epsilon 280AMCA).A350 where A is the optical density of the conjugate at the given wavelength and epsilon is the extinction coefficient of a substance at the wavelength specified. Using conjugates of model proteins, it was found that the extinction coefficients of the AMCA moiety of AMCA-conjugated protein were 1.90 x 10(4) at 350 nm and 8.29 x 10(3) at 280 nm. Similarly, it was found that the extinction coefficients of swine IgG were 1.56 x 10(3) at 350 nm and 1.26 x 10(5) at 280 nm. Thus, for AMCA-conjugated swine IgG: f/p = (1.26 x 10(5)).A350 - (1.56 x 10(3)).A280/(1.47 x 10(4)).A280 - (6.42 x 10(3)).A350 [corrected]. Based on this formula, the f/p ratios of some AMCA-IgG conjugates useful for immunohistochemistry have been found to range between 6 and 24.

scholarly journals A New Spectrophotometric Method for the Determination of Dithianon in Commercial Formulations and Its Residues in Foodstuffs

1999 ◽  
Vol 82 (6) ◽  
pp. 1298-1302 ◽  
Author(s):  
Balbir C Verma ◽  
Hari K Thakur ◽  
Jasvir Singh ◽  
Devender K Sharma
Keyword(s):  
Spectrophotometric Method ◽  
Apple Fruit ◽  
Titration Method ◽  
Red Color ◽  
Beer's Law ◽  
Photometric Titration ◽  
Beer’S Law ◽  
Formulation Analysis

Abstract A new, simple, and rapid spectrophotometric method for the microdetermination of dithianon, on the basis of its reaction with a dithiocarbamate, is described. The red color, which develops instantaneously when mixing the fungicide with the reagent in acetonitrile, is stable for at least 1 h and is measured at 520 nm. Beer's law is applicable up to 12 μg/mL dithianon concentration. The method has been successfully adapted to the analysis of the fungicide in commercial formulations and its residues on grains and apple (fruit and leaves). A photometric titration method for formulation analysis of the fungicide has also been developed.

scholarly journals Rapid and Selective UV Spectrophotometric Method for the Analysis of Ceftazidime

2009 ◽  
Vol 92 (3) ◽  
pp. 820-823 ◽  
Author(s):  
Andréia de Haro Moreno ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Quantitative Evaluation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pure Form ◽  
Uv Detector ◽  
Relative Standard ◽  
Beer's Law ◽  
Beer’S Law

Abstract A new UV spectrophotometric method was developed for quantitative evaluation of ceftazidime preparations. The UV detector was set at 255 nm. Beer's law is obeyed in the concentration range of 7.014.0 g/mL. The method was found to be selective, linear, accurate, and precise in the specified ranges. Intra- and interday variability for the method were <2 relative standard deviation. Common excipients used as additives in pharmaceutical preparations do not interfere with the proposed method. This method was successfully used for quantification of ceftazidime in pure form and in pharmaceutical preparations.

Spectrophotometric Determination of Lanthanum in Raffinate with Tribromoarsenazo

2014 ◽  
Vol 675-677 ◽  
pp. 1429-1432
Author(s):  
Xing Xin Chen ◽  
Kui Liu ◽  
Li Fang Kuang ◽  
Hua Long
Keyword(s):  
Ascorbic Acid ◽  
Hydrochloric Acid ◽  
Correlation Coefficient ◽  
Wavelength Range ◽  
Spectrophotometric Determination ◽  
Linear Relation ◽  
Acidic Environment ◽  
Beer's Law ◽  
Beer’S Law

Determination of lanthanum in the raffinate by spectrophotometry was studied, using tribromoarsenazo as chromogenic reagent. The addition of ascorbic acid can mask the interference of other metal cations in the solution. Under the conditions of ascorbic acid (10g·L-1) 5 mL, hydrochloric acid 0.04 mol·L-1 and tribromoarsenazo (0.5g·L-1) 2 mL, Beer’s law is obeyed in the range of 0-10 μg·(25mL)-1 for lanthanum, with correlation coefficient higher than 0.99. Beer’s law can be applicable for the wavelength range of 580-660 nm. There is no linear relation at too high or too low acidic environment.

scholarly journals New Spectrophotometric Determination of Cefdinir by Batch Method and Cloud Point Extraction

2019 ◽  
Vol 30 (1) ◽  
pp. 85
Author(s):  
Omar Qusay Mezhir
Keyword(s):  
Detection Limit ◽  
Correlation Coefficient ◽  
Cloud Point ◽  
Azo Dye ◽  
Cloud Point Extraction ◽  
Diazonium Salt ◽  
Molar Absorptivity ◽  
Purple Colour ◽  
Beer's Law ◽  
Beer’S Law

In this study, new spectrophotometric methods were developed for estimation of cefdinir (CFD); the research consists of two methods. The first method depends on the conversion of function group amine in cefdinir to diazonium salt and then combined with the 2, 5-Dimethylphenol (2, 5-DMP) reagent in the alkaline medium. The formed azo dye has a purple colour with absorption intensity at λmax 510 nm. Concentration range was obeyed Beer's law at (1-50 μg / ml), correlation coefficient was 0.9998, molar absorptivity was 1.54 × 104 L.mol-1.cm-1 and the detection limit was 0.04 μg.ml-1. The second method is including cloud point extraction (CPE) of a trace amount of the formed azo dye in the first method followed by measuring with a UV-visible spectrophotometer. Concentration range that obeyed the Beer's law was (0.2-6 μg / ml), correlation coefficient was 0.9998, molar absorptivity was1.48×105 L.mol-1.cm-1, detection limit was 0.004 μg.ml-1, Pre-concentration factor was 25 and Distribution coefficient was 125.The proposed methods were applied and proved their compatibility for estimating of ingredient compound in pure samples and pharmaceuticals by comparing them with previous studies. Keyword: cefdinir, diazotization, cloud point extraction, 2, 5-Dimethylphenol.

scholarly journals ACADEMIC ATMOSPHERE, PROVISION OF FACILITIES AND ITS EFFECT ON THE SPIRIT OF PERMANENT LECTURERS AT STIE TRISNA NEGARA OKU TIMUR

2020 ◽  
Vol 4 (03) ◽  
Author(s):  
Winda Feriyana
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Regression Model ◽  
Multiple Linear Regression ◽  
Correlation Coefficient ◽  
Confidence Level ◽  
Analytical Method ◽  
Linear Regression Equation ◽  
Spss Software ◽  
Dominant Influence

This research aims to partially and collectively analyze the influence of the academic atmosphere, the provision of facilities on the work spirit of permanent lecturers and to analyze the most dominant variable in influencing the work spirit of permanent lecturers at STIE Trisna Negara OKU Timur. The regression analysis results and the correlation between academic atmosphere and employee work spirit show the regression model Ŷ = 21,908 + 0.551X1 + e with a correlation coefficient of 0.589. The results of the regression analysis and the correlation between the provision of facilities on employee work spirit show the regression model Ŷ = 46.446 + 0.027X2 + e with a correlation coefficient of 0.033. The results of multiple regression analysis and the correlation between the academic atmosphere and the provision of facilities together on work spirit show the regression model Ŷ = 19.792 + 0.553X1 + 0.043X2 + e with a correlation coefficient of 0.592 at the 95% confidence level, it is found that the academic atmosphere and provision facilities can simultaneously predict employee work spirit. From the multiple linear regression equation above, it shows that the academic atmosphere variable (X1) has a more dominant influence on employee work spirit than the provision of facilities. This research was conducted on 45 respondents with the analytical method used is path analysis using SPSS software. Keywords : Academic Atmosphere, Facility Provision, Work spirit

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