scholarly journals Endobronchial metastasis of urinary bladder carcinoma

2021 ◽  
Vol 2 (5) ◽  
Author(s):  
Yusuf Taha Güllü ◽  
◽  
Büşra Adıgüzel Gündoğdu ◽  
Tibel Tuna ◽  
Nurhan Köksal ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Bladder Carcinoma ◽  
Cancer Metastasis ◽  
Pulmonary Involvement ◽  
Urinary Bladder Carcinoma ◽  
Common Symptom ◽  
Cancer Type ◽  
Endobronchial Metastasis ◽  
Multiple Biopsies

Introduction: Bladder cancer is the 12th most common cancer type worldwide. The most common symptom is painless hematuria. The most common sites of distant metastasis are liver (47%), lung (45%) and bone (32%), respectively. Case report: 73-year-old male patient was admitted with the complaint of hematuria. With a preliminary diagnosis of bladder cancer transurethral resection of tumour (TUR-B) was performed and pathology reported invasive urothelial carcinoma. The patient applied to the pulmonary diseases outpatient clinic with the complaint of cough and wheezing. Bronchoscopy was performed with a pre-diagnosis of bladder cancer metastasis. Multiple biopsies from EBL reported in accordance with urothelial carcinoma metastasis. Result: Urothelial carcinoma of the bladder may present with different pulmonary involvement. Although endobronchial metastasis is a rare condition; it should always be kept in mind in patients with respiratory system complaints. Keywords: endobronchial metastasis; bladder carcinoma; urothelial carcinoma; pulmonary involvement.

scholarly journals ITPR3 Facilitates Tumor Growth, Metastasis and Stemness by Inducing the NF-ĸB/CD44 Pathway in Urinary Bladder Carcinoma

2020 ◽  
Author(s):  
Mengzhao Zhang ◽  
Lu Wang ◽  
Yangyang Yue ◽  
Lu Zhang ◽  
Tianjie Liu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Bladder Carcinoma ◽  
Cancer Metastasis ◽  
Critical Role ◽  
Cancer Tissue ◽  
Urinary Bladder Carcinoma ◽  
Mesenchymal Transition ◽  
Bladder Cancer Cells

Abstract Background: Bladder carcinoma is one of the most common urological cancers. ITPR3, as a ubiquitous endoplasmic reticulum calcium channel protein, was reported to be involved in the development and progression of various types of cancer. However, the potential roles and molecular mechanism of ITPR3 in bladder cancer are still unclear. Herein, we elucidated a novel role of ITPR3 in regulating the proliferation, metastasis, and stemness of bladder cancer cells.Methods: The expression of ITPR3 in bladder cancer was analyzed using public databases and bladder cancer tissue microarrays. To demonstrate the role of ITPR3 in regulating the NF-ĸB/CD44 pathway and the progression of bladder cancer, a series of molecular biology and biochemistry methods was performed on clinical tissues, along with in vivo and in vitro experiments. The methods used included western blot assay, quantitative RT-PCR assay, immunofluorescence assay, immunohistochemistry (IHC) assays, wound healing assay, Transwell assay, colony formation assay, tumorsphere formation assay, cell flow cytometry analysis, EdU assay, MTT assay, cell transfection, bisulfite sequencing PCR (BSP), a xenograft tumor model and a tail vein cancer metastasis model.Results: Higher ITPR3 expression was found in bladder cancer tissues and bladder cancer cells compared with the corresponding normal peritumor tissues and SV-HUC-1 cells, which was attributed to demethylation in the ITPR3 promoter region. ITPR3 promoted the proliferation of bladder cancer by accelerating cell cycle transformation and promoted local invasion and distant metastasis by inducing epithelial-to-mesenchymal transition (EMT). Meanwhile, ITPR3 maintained the cancer stemness phenotype by regulating CD44 expression. NF-κB, which is upstream of CD44, also played a critical role in this process.Conclusions: Our study clarifies that ITPR3 serves as an oncogene in bladder cancer cells and represents a novel candidate for bladder cancer diagnosis and treatment.

scholarly journals ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma

2021 ◽  
Author(s):  
Mengzhao Zhang ◽  
Lu Wang ◽  
Yangyang Yue ◽  
Lu Zhang ◽  
Tianjie Liu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Bladder Carcinoma ◽  
Cancer Metastasis ◽  
Critical Role ◽  
Cancer Tissue ◽  
Urinary Bladder Carcinoma ◽  
Mesenchymal Transition ◽  
Bladder Cancer Cells

Abstract Background: Bladder carcinoma is one of the most common urological cancers. ITPR3, as a ubiquitous endoplasmic reticulum calcium channel protein, was reported to be involved in the development and progression of various types of cancer. However, the potential roles and molecular mechanism of ITPR3 in bladder cancer are still unclear. Herein, we elucidated a novel role of ITPR3 in regulating the proliferation, metastasis, and stemness of bladder cancer cells.Methods: The expression of ITPR3 in bladder cancer was analyzed using public databases and bladder cancer tissue microarrays. To demonstrate the role of ITPR3 in regulating the NF-ĸB/CD44 pathway and the progression of bladder cancer, a series of molecular biology and biochemistry methods was performed on clinical tissues, along with in vivo and in vitro experiments. The methods used included western blot assay, quantitative RT-PCR assay, immunofluorescence assay, immunohistochemistry (IHC) assays, wound healing assay, Transwell assay, colony formation assay, tumorsphere formation assay, cell flow cytometry analysis, EdU assay, MTT assay, cell transfection, bisulfite sequencing PCR (BSP), a xenograft tumor model and a tail vein cancer metastasis model.Results: Higher ITPR3 expression was found in bladder cancer tissues and bladder cancer cells compared with the corresponding normal peritumor tissues and SV-HUC-1 cells, which was attributed to demethylation in the ITPR3 promoter region. ITPR3 promoted the proliferation of bladder cancer by accelerating cell cycle transformation and promoted local invasion and distant metastasis by inducing epithelial-to-mesenchymal transition (EMT). Meanwhile, ITPR3 maintained the cancer stemness phenotype by regulating CD44 expression. NF-κB, which is upstream of CD44, also played a critical role in this process.Conclusions: Our study clarifies that ITPR3 serves as an oncogene in bladder cancer cells and represents a novel candidate for bladder cancer diagnosis and treatment.

scholarly journals Expression and Clinical Significance of MMP-28 in Bladder Cancer

2020 ◽  
Vol 19 ◽  
pp. 153303382097401
Author(s):  
Heng Wang ◽  
Jun-xiu Wu ◽  
Xin-Peng Chen ◽  
Qi Zhang ◽  
Hai-bin Wei ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Cancer Progression ◽  
Bladder Carcinoma ◽  
Clinicopathological Characteristics ◽  
Urinary Bladder Carcinoma ◽  
Normal Bladder ◽  
Patient Prognosis ◽  
High Level ◽  
Risk Of Cancer

Aims: The aim of this study to determine the expression of MMP-28 in bladder urothelial carcinoma and to analyze the correlation between MMP-28 and the clinicopathological characteristics of human bladder carcinoma, and its relationship with patient prognosis. Methods: A total of 491 surgically resected bladder cancer samples and 80 normal tissue adjacent to the tumor were stained by immunohistochemistry. The expression of MMP-28 in these samples was quantitated, and the value of MMP-28 as a marker of bladder cancer and prognosis was assessed. Results: The expression of MMP-28 in urinary bladder carcinoma was higher than in normal bladder mucosa. The high level of MMP-28 was significantly correlated with tumor histology grade, lymphatic metastasis, lymph node infiltration, and distant metastasis (P < 0.05). The upregulation of MMP-28 was also closely related to the risk of cancer progression and the survival of patients. Further analysis documented that high expression of MMP-28 was associated with decreased overall survival in bladder cancer patients. Conclusions: The abnormal expression of MMP-28 may be related to the initiation and development of urothelial carcinoma. The upregulation of MMP-28 can be used as one of the effective indicators to diagnose bladder cancer and predict tumor progression.

scholarly journals ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Mengzhao Zhang ◽  
Lu Wang ◽  
Yangyang Yue ◽  
Lu Zhang ◽  
Tianjie Liu ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Bladder Carcinoma ◽  
Cancer Metastasis ◽  
Critical Role ◽  
Cancer Tissue ◽  
Urinary Bladder Carcinoma ◽  
Mesenchymal Transition ◽  
Bladder Cancer Cells

Abstract Background Bladder carcinoma is one of the most common urological cancers. ITPR3, as a ubiquitous endoplasmic reticulum calcium channel protein, was reported to be involved in the development and progression of various types of cancer. However, the potential roles and molecular mechanism of ITPR3 in bladder cancer are still unclear. Herein, we elucidated a novel role of ITPR3 in regulating the proliferation, metastasis, and stemness of bladder cancer cells. Methods The expression of ITPR3 in bladder cancer was analyzed using public databases and bladder cancer tissue microarrays. To demonstrate the role of ITPR3 in regulating the NF-ĸB/CD44 pathway and the progression of bladder cancer, a series of molecular biology and biochemistry methods was performed on clinical tissues, along with in vivo and in vitro experiments. The methods used included western blot assay, quantitative RT-PCR assay, immunofluorescence assay, immunohistochemistry (IHC) assays, wound healing assay, Transwell assay, colony formation assay, tumorsphere formation assay, cell flow cytometry analysis, EdU assay, MTT assay, cell transfection, bisulfite sequencing PCR (BSP), a xenograft tumor model and a tail vein cancer metastasis model. Results Higher ITPR3 expression was found in bladder cancer tissues and bladder cancer cells compared with the corresponding normal peritumor tissues and SV-HUC-1 cells, which was attributed to demethylation in the ITPR3 promoter region. ITPR3 promoted the proliferation of bladder cancer by accelerating cell cycle transformation and promoted local invasion and distant metastasis by inducing epithelial-to-mesenchymal transition (EMT). Meanwhile, ITPR3 maintained the cancer stemness phenotype by regulating CD44 expression. NF-κB, which is upstream of CD44, also played a critical role in this process. Conclusions Our study clarifies that ITPR3 serves as an oncogene in bladder cancer cells and represents a novel candidate for bladder cancer diagnosis and treatment.

scholarly journals Expression of HER2/neu and Ki-67 in Urothelial Carcinoma and their Relation to Clinicopathological Data: An Egyptian Study

2021 ◽  
Author(s):  
Badawia B Ibrahim ◽  
Samira A Mahmoud ◽  
Alzahraa A Mohamed ◽  
Hala M El Hanbuli
Keyword(s):  
Urothelial Carcinoma ◽  
Bladder Carcinoma ◽  
Histological Grade ◽  
P Value ◽  
Urinary Bladder Carcinoma ◽  
Ki 67 ◽  
Urothelial Bladder Carcinoma ◽  
Cellular Marker ◽  
Urothelial Bladder ◽  
Common Malignancy

Introduction: Bladder cancer is the most common malignancy involving the urinary system and the ninth most common malignancy worldwide. Ki-67 is a nonhistone cellular marker for proliferation. HER2/neu is an oncogene that plays an important role in the pathogenesis of many cancer types. In bladder carcinoma, its clinical significance remains under-investigated and poorly linked to the patients’ clinicopathological features especially with no reported Egyptian study. Aim: The aim of this work was to study the expression of HER2/neu and Ki-67 in urinary bladder carcinoma to evaluate their role in tumourigenesis and their correlation with other available clinicopathological variables associated with urothelial carcinoma. Materials and Methods: This cross-sectional study was conducted at the Department of Pathology, Faculty of Medicine, Cairo University, Egypt. Samples were paraffin blocks from 60 cases diagnosed with urothelial carcinoma underwent radical cystectomy. Ki-67 and HER2/neu immunohistochemical staining was done and of Ki-67 and HER2/neu Immunostaining was recorded. The associations between Ki-67, HER2/neu expressions and clinical and histopathological parameters of urothelial bladder carcinoma was evaluated. Results: The Ki-67 expression had significant association with tumour histological grade and lymphovascular invasion (p-value <0.05). The association of HER2/neu expression had significant association with perineural invasion (p-value <0.05). Conclusion: HER2/neu immunostaining was not associated with most of the clinicopathologic prognostic factors in urothelial bladder carcinoma.

scholarly journals Urothelial bladder carcinoma with major clinical presentation as overactive bladder, without hematuria: case report and literature review

2018 ◽  
Vol 7 (10) ◽  
pp. 4292
Author(s):  
Alexander de J. Rafaelano M. ◽  
Junior J. Araiza Navarro ◽  
María Isabel Tolentino Sosa ◽  
Fernando López Reyes ◽  
Marlene De la Peña Gutiérrez
Keyword(s):  
Bladder Cancer ◽  
Case Report ◽  
Urothelial Carcinoma ◽  
Cell Carcinoma ◽  
Transitional Cell ◽  
The United States ◽  
Transitional Epithelium ◽  
Common Symptom ◽  
Macroscopic Hematuria ◽  
Adjacent Structures

The urothelial carcinoma is the most common type of bladder cancer, comprising approximately 90% of cases in the United States. The most common symptom of bladder cancer is macroscopic hematuria, increased urinary frequency, urgency, or irritative symptoms may occur. Generally, occurs in elderly people, about 9 out of 10 people are over 55 years old, with the average age at diagnosis of 73 years. Males are more likely than women to have this neoplasm with a probability of 1 in 27 (for women the probability is 1 in 89). Most bladder cancers begin in the inner layer, also called the urothelium or transitional epithelium. As it advances, it invades the layers of the bladder and can invade adjacent structures, often metastasizing to distant lymph nodes, bones, lungs or the liver. Among the cancers that originate in the bladder authors have: Urothelial carcinoma (transitional cell carcinoma), squamous cell carcinoma, adenocarcinoma, small cell carcinoma, sarcoma. Hematuria occurs in the majority of patients with urothelial carcinoma. Symptoms such as dysuria, frequency, urgency and pain may also occur, or it may also be asymptomatic.  In this case report, an atypical presentation of bladder cancer is shown, simulating the symptomatology of a Hyperactive Bladder Syndrome.

scholarly journals Bladder Cancer Metastasis Induced by Chronic Everolimus Application Can Be Counteracted by Sulforaphane In Vitro

2020 ◽  
Vol 21 (15) ◽  
pp. 5582
Author(s):  
Saira Justin ◽  
Jochen Rutz ◽  
Sebastian Maxeiner ◽  
Felix K.-H. Chun ◽  
Eva Juengel ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Bladder Carcinoma ◽  
Cancer Metastasis ◽  
Chemotactic Activity ◽  
Carcinoma Cells ◽  
Down Regulation ◽  
Knock Down ◽  
Tumor Dissemination ◽  
Bladder Carcinoma Cells

Chronic treatment with the mTOR inhibitor, everolimus, fails long-term in preventing tumor growth and dissemination in cancer patients. Thus, patients experiencing treatment resistance seek complementary measures, hoping to improve therapeutic efficacy. This study investigated metastatic characteristics of bladder carcinoma cells exposed to everolimus combined with the isothiocyanate sulforaphane (SFN), which has been shown to exert cancer inhibiting properties. RT112, UMUC3, or TCCSUP bladder carcinoma cells were exposed short- (24 h) or long-term (8 weeks) to everolimus (0.5 nM) or SFN (2.5 µM), alone or in combination. Adhesion and chemotaxis along with profiling details of CD44 receptor variants (v) and integrin α and β subtypes were evaluated. The functional impact of CD44 and integrins was explored by blocking studies and siRNA knock-down. Long-term exposure to everolimus enhanced chemotactic activity, whereas long-term exposure to SFN or the SFN-everolimus combination diminished chemotaxis. CD44v4 and v7 increased on RT112 cells following exposure to SFN or SFN-everolimus. Up-regulation of the integrins α6, αV, and β1 and down-regulation of β4 that was present with everolimus alone could be prevented by combining SFN and everolimus. Down-regulation of αV, β1, and β4 reduced chemotactic activity, whereas knock-down of CD44 correlated with enhanced chemotaxis. SFN could, therefore, inhibit resistance-related tumor dissemination during everolimus-based bladder cancer treatment.

scholarly journals Etiological study of urinary bladder carcinoma in patients presenting to tertiary care centre

2020 ◽  
Vol 8 (1) ◽  
pp. 43
Author(s):  
Gurpreet Singh Bhangu ◽  
Ripudaman Singh ◽  
Darpan Bansal ◽  
Balcharan S. Bajwa
Keyword(s):  
Bladder Cancer ◽  
Blood Group ◽  
Bladder Carcinoma ◽  
Tertiary Care ◽  
Urinary Bladder Carcinoma ◽  
Tertiary Care Centre ◽  
Etiological Factors ◽  
Wide Range ◽  
Carcinogenic Substances ◽  
Etiological Study

Background: Urothelial carcinoma is the most common invasive cancer of the urinary tract. Lately, there has been an increased incidence of urothelial neoplasia due to exposure to a wide range of potentially carcinogenic substances. Studies of involved factors led to the concept of existence of a so-called malignization terrain, which claims that individual genetic predisposition and chronic exposure to carcinogens act synergistically leading to the appearance of urothelial carcinomas of the bladder. Aim of the research was to find out the common etiological factors of bladder cancer in this part of India.Methods: The study included 100 patients of bladder carcinoma reporting to Sri Guru Ram Das Hospital, Amritsar from March 2018 to December 2019. A detailed history was taken to have the insight of various etiological factors of the disease. The data was entered in Microsoft excel spreadsheet and analysis was done using statistical package for social sciences (SPSS) version 21.0.Results: The most common blood group associated with CA UB was A +ve (39%) followed by B +ve (29%). 89% of the cases of CA UB were non-smokers predominantly attributed to type of patients coming to our tertiary care institute which are from a rural background (73%) and are mostly Sikhs (80%) and Sikhs are traditionally non-smokers. 80% were farmers by occupation who have exposure to pesticides, insecticides, weedicides and herbicides routinely.Conclusion: In our study majority of the patients turned out to be non-smokers and A +ve blood group in contrast to the strong predilection of smoking and bladder cancer. 

scholarly journals Urinary Bladder Carcinoma: Impact of Smoking, Age and its Clinico-Pathological Spectrum

2015 ◽  
Vol 11 (4) ◽  
pp. 292-295 ◽  
Author(s):  
HN Joshi ◽  
R Makaju ◽  
A Karmacharya ◽  
RM Kamracharya ◽  
B Shrestha ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Occupational Exposure ◽  
Urinary Bladder ◽  
Bladder Carcinoma ◽  
Urinary Bladder Cancer ◽  
Aetiological Factor ◽  
Urinary Bladder Carcinoma ◽  
Chemical Carcinogen ◽  
History Of ◽  
The Impact

Background Urinary bladder carcinoma is common urological malignancy. Although epidemiological evidence favors role of occupational exposure to chemical carcinogen as the aetiological factor of bladder carcinoma, many cases arise with no obvious occupational exposure to chemical carcinogen. Tobacco and cigarette smoking is common in both rural and urban areas of Nepal.Objective The objective of this study was to determine the impact of smoking and age in urinary bladder carcinoma with related clinicopathological correlations.Method A total of 56 (44 males and 12 females) cases of urinary bladder cancer treated at Dhulikhel Hospital, Kathmandu University Teaching Hospital during time period of January 2004 to December 2013 were included in the study. Data of patients with Urinary bladder cancer were obtained from hospital records and evaluated for age, sex, history of smoking, clinical presentations, cystoscopic findings and histopathological characteristics.Results Out of 56 cases, 51 (91.1%) of the patients had hematuria. History of smoking was found in 44 patients. Smoking was found much higher in males (88%) than females (41.66%). Transitional cell carcinoma (TCC) was the most common histological variety, which was seen in 51 (91.07%) patients. The significant impact of smoking was found in terms of grade of TCC.Conclusion The incidence of bladder carcinoma is higher in male and TCC is the most common variety of Urinary bladder malignancy. History of smoking correlated with grade.Kathmandu Univ Med J 2013; 11(4): 292-295

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