Study into the Iron Content of Seminal Plasma in Normal and Infertile Subjects

2012 ◽  
Vol 79 (1) ◽  
pp. 54-57 ◽  
Author(s):  
KP Skandhan ◽  
BN Mazumdar ◽  
B Sumangala

The iron content in seminal plasma of normal (n19), oligozoospermic (n11), azoospermic (n12), oligoasthenozoospermic (n19), and asthenozoospermic (n17) subjects was estimated by using atomic absorption spectrophotometer. The concentration of iron in normal seminal plasma varied from 265 to 365 mg%. The source of iron in seminal plasma seems to be the adnexal glands and not spermatozoa, as azoospermic semen also contained it. A statistically highly significant difference was seen when normal was compared with azoospermia and with asthenozoospermia. The necessary average wastage of iron through semen is calculated as 2.52 mg/day. This value is highly variable according to the seminal volume and frequency of ejaculation.

Author(s):  
Bushra Aziz ◽  
Muhammad Zubair ◽  
Nausheen Irshad ◽  
Khawaja Shafique Ahmad ◽  
Majid Mahmood ◽  
...  

AbstractThe current study was designed to determine the concentrations of toxic metals (Ni, Pb and Cr) in feathers of birds collected from four regions of NE Pakistan. Feather samples of birds (House Crow, Common Myna and House Sparrow) were collected from different areas. Atomic absorption spectrophotometer was used to determine the concentration of metals in feathers. Analysis of the data revealed that concentrations of Pb and Cr were significantly different (p < 0.05) among bird species, whereas no difference (p > 0.05) was detected among bird species (house crow, common myna and house sparrow) for Ni. A significant difference was found for the concentration of Pb and Ni in all the four studied regions. Whereas, non-significant difference was found in all the studied regions for the concentrating of Cr. It was revealed that there is significant rising concentration of metals (Pb, Cr) in feathers of birds in Azad Kashmir.


2019 ◽  
Vol 26 (2) ◽  
pp. 190 ◽  
Author(s):  
Lydia Fanny

ABSTRACT Children under five begin to have difficulty eating or just like snacks that are classified as empty calories and nutrition in general.  Therefore it is necessary to enrich local snacks as an alternative for handling nutritional problems.  One of the local snacks that can help improve nutritional problems is serabi with the addition of tempe flour and anchovy flour.  This study aims to determine the iron content of serabi with the addition of tempe flour and anchovy flour.  This research is a type of laboratory research in the field of food.  The serabi formula with the addition of formula concentrations of tempe flour and anchovy flour are F1 (10%: 5%), F2 (15%: 5%), and F3 (25%: 5%).  The method of analysis of iron content is AAS (Atomic Absorption Spectrophotometer).  The data obtained were analyzed by computer programs using the Friedman test.  The results showed that the iron content was highest at a concentration of 10%: 5%.  The addition of tempe flour and anchovy flour did not have a significant effect on color, aroma, texture and taste.  From the most preferred aspect of serabi color on the addition of tempe flour and anchovy flour the concentration of 10%: 5% while the most preferred aspects of texture, aroma and taste on the addition of tempe flour and anchovy flour concentration of 10%: 5%, 15%:  5%, and 25%: 5%.  It can be concluded that the addition of tempe flour and anchovy flour gives increased levels of iron in the serabi.  The addition of tempe flour and anchovy flour do not have a significant effect on color, aroma, texture and taste in all three concentrations.Keywords: Serabi, tempe flour, anchovy flour


2020 ◽  
Vol 24 (6) ◽  
pp. 1035-1040
Author(s):  
D.C. Sakiyo ◽  
G. Chessed ◽  
J. Eli ◽  
Y.J. Usongo

The study analyses the health risk assessment of the concentration of Iron, Lead, Copper, Chromium, and Cadmium heavy metals in vegetables grown near dumpsites of Jimeta and Ngurore areas of Adamawa State, Nigeria. Vegetables mainly Spinach (Spinacia oleracea) and lettuce (Lactuca sativa) were collected in triplicates and analyzed using Atomic Absorption Spectrophotometer Buck 210VGP (AAS). The result revealed that heavy metals detected in spinach at Jimeta dumpsite decreased in the following order: Fe (3.7 mg/kg) > Pb (0.18 mg/kg) > Cu (0.12 mg/kg) > Cr (0.07 mg/kg) > Cd (below limit of detection), compared to the metal concentration in spinach at Ngorure dumpsite with lower concentration of heavy metal which decreased in the order of: Fe (2.5 mg/kg) > Pb (0.16 mg/kg) > Cu (0.14 mg/kg) > Cr (0.02 mg/kg) > Cd (below limit of detection). Other result for Spinach in Jimeta decreased in the order Fe (3.31mg/kg) > Pb (0.2mg/kg) > Cu (0.11mg/kg) > Cr (0.05mg/kg) > Cd (ND) beyond the limit of detection while Lettuce decreased in the order Fe (22.54mg/kg) > Cu (0.31mg/kg) > Pb (0.12mg/kg) > Cr (0.07mg/kg) > Cd (ND) beyond the limit of detection. Fe is the most abundant element in the vegetables with a mean value of 21 mg/kg followed by Pb (0.177 mg/kg). The analyses of paired T-test for vegetables in Ngurore and Jimeta at 0.05 level of significant confirmed that Cu and Cr showed no statistically significant difference in their concentration level while Fe and Pb confirmed that there was statistically significant difference in their concentration level. However, the detection of heavy metals in these vegetables calls for close environmental monitoring and adequate public awareness. This is necessary to discourage further pollution which could lead to high metal concentration and metal poisoning in vegetables and invariably humans that consume them. Keywords: Spinacia oleracea, Lactuca sativa, Heavy metal, Atomic Absorption Spectrophotometer


2020 ◽  
Vol 15 (2) ◽  
pp. 137
Author(s):  
Rahmawati Rahmawati ◽  
Wa Ode Rustiah ◽  
Nurhidayat Nurhidayat ◽  
Novie Rezkiyana Dewi

Shellfish is one of the sea products that is very popular with the community including the community in Sinjai Regency as a potential coastal and fishery resources that have a fish auction place (TPI). Shellfish are one of the most efficient marine animals to accumulate heavy metals. This is due to the fact that shellfish live in the bottom sedimentary layer of water, move very slowly, and their food comes from detritus in the bottom waters, so the chance of entering heavy metals into the body is very large. Shells can be contaminated by lead (Pb) which enters the body of water through the crystallization of lead in the air with the help of rain water. Lead which accumulates in water and sediments will enter the water and accumulate in the water causing toxic effects on the organisms in it, including some types of shellfish. If humans consume shells that contain heavy metals in high enough quantities will have a negative impact on health. In the human body, heavy metals will combine with active enzymes to become inactive enzymes, so the synthesis of red blood grains (Hb) can be inhibited, consequently it can cause anemia. The purpose of the study was to determine lead content (Pb) in the sample of shellfish. The object of research is blood shells and mangrove shells, analyzed using the Atomic Absorption Spectrophotometer (SSA) method with a total sample of 10. The results obtained by lead (Pb) in blood shells (Anadara granosa) with an average of 0.0439 mg / kg while the mangrove shells (Telescopium telescopium) with an average of 0.0176 mg / kg. Both shellfish samples did not exceed the maximum limit set by the Indonesian National Standard (SNI) of 1.5 mg / kg. Based on the data analysis test p value = 0.011 <0.05 which can be concluded that there is a significant difference between the levels of lead (Pb) in blood shells (Anadara granosa) and mangrove shells (Telescopium telescopium). Keywords: Blood Shells (Anadara granosa), Mangrove Shells (Telescopium telescopium), Lead (Pb),                  Atomic Absorption Spectrophotometer (SSA)


2019 ◽  
Vol 8 (4) ◽  
pp. 204-208
Author(s):  
Betrilia N. Bandola ◽  
Mery Napitupulu ◽  
Baharuddin Hamzah

Gedi is one of the common plants grown in Sulawesi. People recognize the two types of gedi, namely, green gedi and red gedi. The use of red gedi leaves is traditionally believed to cure several diseases, one of these diseases is anemia and as an alternative treatment such as to prevent heart disease. This study aimed to determine the levels of iron (Fe) and potassium (K) that contains in red gedi leaves (Abelmoschus manihot L.). Iron and potassium contents were determined using an atomic absorption spectrophotometer (SSA). The average iron content of red gedi leaves from Tentena was 9,760 mg/100 g and from Lasoani was 9,668 mg/100 g while the average potassium content of the red gedi leaves from Tentena was 3,882 mg/100g and from Lasoani was 3,445 mg/100 g.


2020 ◽  
Vol 71 (8) ◽  
pp. 240-248
Author(s):  
Kafeel Ahmad ◽  
Yongjun Yang ◽  
Zafar Iqbal Khan ◽  
Nimra Arshad ◽  
Tasneem Ahmad ◽  
...  

The article is focused on estimation of Zinc in the roadside forages, and blood of buffaloes feeding on these forages. This study was carried out in Sargodha during December 2015 to January 2016 (winter) and May 2016 to June 2016 (summer). Five road sites (Mateela, Faisalabad roadside, Shaheenabad roadside, Bhalwal roadside and 50 chak) were selected from sampling of forages, soil and buffalo blood sample. Heavy metal analysis of all digested samples was done with atomic absorption spectrophotometer. Analysis of variance and correlation was done with two way ANOVA. This study regarding the accumulation of zinc in forages, soil and the buffalo blood would help the authorities to exactly determine the agents which are responsible for increasing pollution in the environment.


Reproduction ◽  
2001 ◽  
pp. 431-436 ◽  
Author(s):  
U Sharma ◽  
K Chaudhury ◽  
NR Jagannathan ◽  
SK Guha

Nuclear magnetic resonance (NMR) spectroscopy was used to quantify citrate, glucose, lactate, glycerophosphorylcholine and choline in seminal plasma from subjects injected with a new male contraceptive RISUG, a copolymer of styrene maleic anhydride dissolved in dimethyl sulphoxide, and in seminal plasma from normal ejaculates. No significant difference in the concentration of citrate was observed between the groups, indicating that the prostate is not affected by the contraceptive. The concentrations of glucose, lactate, glycerophosphorylcholine and choline were significantly lower (P < 0.01) in subjects injected with RISUG compared with controls. In addition, metabolite ratios such as choline:citrate, citrate:lactate, choline:lactate and glycerophosphorylcholine:choline were calculated. Citrate:lactate and glycerophosphorylcholine:choline ratios were significantly lower in RISUG-injected subjects than in controls (P < 0.01), thereby indicating the occurrence of partial obstructive azoospermia. The most important finding of the present study was that the intervention of RISUG in the vas deferens even for a period as long as 8 years is absolutely safe and does not lead to prostatic diseases.


1986 ◽  
Vol 66 (4) ◽  
pp. 751-755 ◽  
Author(s):  
C. WANG ◽  
P. A. SCHUPPLI

Determination of oxalate-extractable Si and Al is useful in assessing the degree of accumulation of allophane-like materials in Podzolic soils. Three procedures were tested for determining Si: manual spectrophotometry, automated spectrophotometry and atomic absorption. For concentrations of oxalate-extractable Si above 0.5 g kg−1, there was no significant difference among results by the three procedures. For lower concentrations of Si, results by atomic absorption were higher than those by the spectrophotometric procedures. Determination by Si by autoanalyzer is the preferred procedure as it is convenient, sensitive and reliable. Key words: Oxalate Si, Podzolic soil, autoanalyzer


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