scholarly journals EVALUATION OF MICROBIAL LOAD AND SELECTED HEAVY METALS CONTAMINATION IN THE RIVER BEAS (PUNJAB) INDIA

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Baljot Singh Maan ◽  
Syed Shabih Hassan
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Microbial Load ◽  
Plate Count ◽  
Standard Protocol ◽  
Faecal Coliforms ◽  
Total Plate Count ◽  
Lead And Zinc ◽  
Nickel Lead ◽  
River Beas

Aim: To analyse the heavy metal contamination and microbial load in upstream (Talwara), midstream (Urmar Tanda) and downstream (Harike Pattan) stretches of the river Beas in Punjab. Methodology: Month-wise samples were collected in triplicate during the period of May to December, 2019. Heavy metal parameters such as Arsenic, Cadmium, Nickel, Lead and Zinc were analysed by Atomic Inductively Coupled Plasma Mass Spectrometry following standard protocol. Microbial load in water samples was calculated in terms of Total Plate Count, Total Coliform and Faecal Coliform as per following standard protocol. Statistical analysis involved calculating Analysis of Variance at 5% significant level (p<0.05). Results: The study revealed that heavy metal parameters like Arsenic, Cadmium, Nickel, Lead and Zinc ranged in mgl from 0.0 to 0.009, 0.0 to 0.02, 0.0 to 0.004, 0.0 to 0.001, and 0.0 to 0.036 respectively. Total Plate Count ranged from 0.32 4 5 -1 -1 x10 to 3.2 x10 cfu ml whereas, Total Coliforms and Faecal Coliforms ranged from 4.0 to 1100 MPN 100 ml and 0.0 to -1 460 MPN 100 ml respectively. The mean concentrations of analysed metals were recorded in the order of Zinc > Cadmium > Arsenic >Nickel >Lead; and most of them were under the permissible limits; however, the presence of faecal coliforms in the water is alarmingly high at Harike Pattan. Interpretation: This investigation revealed that the river Beas water is suitable for supporting aquatic life, bathing, irrigation and other purposes. The microbial load and the heavy metal parameters need to be monitored regularly, so that their adverse effects on living beings or aquatic organisms may be minimized. River stretch near Harike Pattan and Urmar Tanda being an important habitat of endangered freshwater Dolphin (Platanista gangetica minor), Smooth-Coated Otter (Lutrogale perspicilliata) and Ghariyal (Gavialis gangeticus) is more vulnerable to the toxic levels of heavy metals and contamination of faecal coliform.

scholarly journals Research NoteEffect of drought and fires on the quality of water in Lithuanian rivers

2003 ◽  
Vol 7 (3) ◽  
pp. 423-427 ◽  
Author(s):  
G. Sakalauskiene ◽  
G. Ignatavicius
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Peat Bog ◽  
Sudden Increase ◽  
Quality Of Water ◽  
Lead And Zinc ◽  
Lithuanian Rivers ◽  
River Bottom ◽  
Copper Lead

Abstract. In August and September 2002, concentrations of heavy metals (copper, lead, and zinc) were 21-74% more than in previous years in Lithuanian rivers. Such a sudden increase in heavy metal pollution reduces the value of any water body for fishing or recreation and poses a potential risk to the environment and to human health. Droughts in the summer of 2002 led to forest and peat bog fires all over Lithuania and may have caused the increase in concentrations of heavy metals detected in Lithuanian rivers in August 2002. The fires could have changed the pH in the top layers of the soil, overcome geochemical barriers in the soil and enabled heavy metals to migrate from the soil to the groundwater and from river bottom sediments to the surface water. Keywords: heavy metals, river water quality, Lithuania

scholarly journals Organic Versus Conventional – a Comparative Study on Quality and Nutritive Value of Selected Vegetable Crops of Southern India

2020 ◽  
Vol 18 (1) ◽  
pp. 99-116
Author(s):  
JR Xavier ◽  
V Mythri ◽  
R Nagaraj ◽  
VCP Ramakrishna ◽  
PE Patki ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vitamin C ◽  
Metal Contamination ◽  
Nutritive Value ◽  
Heavy Metal Contamination ◽  
Plate Count ◽  
Microbial Quality ◽  
Edible Plant ◽  
Lead And Cadmium

Vegetables are defined as edible plant parts generally consumed raw or cooked with a main dish, in a mixed dish, as an appetizer or as a salad. Food safety aspects related to microbial quality (total plate count, yeast and mold and food borne pathogens) and toxic residue (heavy metals) and mineral content were investigated for vegetables such as green leafy vegetable, salad vegetables, sprouts, brinjal, green chilies and French beans collected from organic and conventional outlets from Mysore region, Karnataka, India. Microbial analysis was carried out using standard procedures and mminerals (Ca, K, Fe, Cu, Mg, Mn and Zn) and heavy metals (Cd and Pb) were determined. Significant variations (p>0.05) were observed for microbial quality among organic and conventional vegetables. Mineral and vitamin C content were also significantly higher (p>0.01) in organic samples. Heavy metal contamination for lead and cadmium tested positive for conventional samples while organic samples tested negative. The variables that contributed most for the variability were heavy metal contamination, mineral and vitamin C content. Organically grown vegetables were free from heavy metals and safe for consumption, as well as they are rich in mineral and vitamin C content in comparison to conventional samples. SAARC J. Agri., 18(1): 99-116 (2020)

Analysis on Pb, Cd, Cr, As, Mn Emission and Control Technology of Typical Industries

2012 ◽  
Vol 610-613 ◽  
pp. 1176-1187 ◽  
Author(s):  
Tao Yue ◽  
Fan Wang ◽  
Bin Jie Han ◽  
Peng Lai Zuo ◽  
Fan Zhang
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Flue Gas ◽  
Power Plants ◽  
Raw Materials ◽  
Control Technology ◽  
Generator System ◽  
Lead And Zinc ◽  
Metal Contents ◽  
Industrial Boilers

The heavy metals including Pb, Cd, Cr, As and Mn emission characteristics of coal-fired power plant boilers, industrial boilers, lead and zinc smelters, cement kilns, and steel industries were studied in this paper. The removing effects of heavy metals by pollution control technology were analyzed. ICP-AES method was used to measure the contents of heavy metals in solid samples. In addition, M-29, the U.S. EPA isokinetic sampling method was used to sample heavy metals in the flue gas, and the sampled heavy metals were tested by ICP-AES-hydride generator system. For coal-fired boilers, heavy metal contents in descending order were Mn, Pb and Cd in the tested coal. The heavy metals’ emission concentrations of coal-fired industrial boilers were much higher than the heavy metals’ emission concentrations of power plants. For the lead and zinc smelters, Pb and its compounds in flue gas after cleaning can meet the emission standards. The heavy metal contents in the cement raw materials in descending order were Pb, Cr and Cd. For the sintering mixture, the heavy metal contents in descending order were Pb, Cr and Cd as well. The removal effects of heavy metals by existing pollutant control technologies in the various typical industries were also tested and analyzed in the paper. The research did in the paper made a scientific basis for controlling heavy metal emissions from typical industries.

Bacteria Counts on Cod and Flounder Fillets Produced Commercially from Fish Frozen at Sea and Thawed in Water

1969 ◽  
Vol 26 (12) ◽  
pp. 3217-3231
Author(s):  
M. J. Hayward ◽  
W. A. MacCallum
Keyword(s):  
Gadus Morhua ◽  
Potable Water ◽  
Plate Count ◽  
Faecal Coliforms ◽  
Buffer Storage ◽  
Total Plate Count ◽  
Processing Line ◽  
Crushed Ice ◽  
Hippoglossoides Platessoides

Fillets of flounder (Hippoglossoides platessoides) and cod (Gadus morhua) produced in two commercial plants from uncut fish that had been frozen in blocks at sea, were free or nearly so of faecal coliforms and generally contained fewer indicator bacteria (all organisms in the coliform group; faecal coliforms) than fillets produced in the same plant from fish iced at sea. Coliforms were found in relatively small numbers on most fillets of thawed fish sampled on the conveyor leading from the mechanical skinner, on similarly prepared fillets sampled after trimming and portioning, and on fillets prepared from fish iced at sea and sampled immediately after skinning. The total plate count on the fillets of thawed fish at the point of trimming and portioning was small, suggesting that the fish frozen at sea carried a relatively low count of fish-spoiling organisms at time of freezing and thawing.The results show that in processing blocks of gutted or gutted and headed fish it is practical to thaw in an immersion thawer at a temperature of 13–20 C while changing the water only infrequently, say every 12 hr, and to employ thawing runs of about 24 hr duration. During this time as many as five tankfuls of fish may be thawed. It is practical also to hold the fish thawed under these conditions in chilled potable water or in crushed ice for 1 or 2 days prior to processing.It was shown that the sanitary aspects of the process can be assessed advantageously by observing the sanitary quality of the fillets, rather than that of the thawing water. The suitability of the method appeared to depend upon efficient washing of the thawed fish before it was put in buffer storage, or before it was filleted, or both. In one of the two plants sampled, large flumes were used and were satisfactory for delivering thawed, washed fish of high bacteriological and sanitary quality from buffer storage to the processing line. Here total plate and coliform counts either increased slightly or decreased slightly with prolonged use of the thawing water and of the boards used in cutting the fillets.

scholarly journals Evaluation of removal of heavy metals by microorganisms isolated from industrial effluents

2017 ◽  
Vol 2 (3) ◽  
pp. 156
Author(s):  
S.A. Bhutada ◽  
S.B. Dahikar
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Concentration ◽  
Mutational Analysis ◽  
Bacterial Species ◽  
Natural Habitat ◽  
Strain Improvement ◽  
Industrial Effluents ◽  
Heavy Metal Concentration ◽  
Plate Count

At present various microorganisms are used for bioremediation of heavy metals from soil and water bodies. The aim of present work was to isolate the potential heavy metal degrading organisms and to apply for bioremediation of heavy metals from the domestic as well as industrial waste. The study involves the isolation of the bacterial species residing the natural habitat of such environments and screening of these isolates to degrade different heavy metals such as Cu, Cd, Hg, Ni, and Zn  up to the concentration 2000 ppm. There were six bacterial potential isolates  found namely Pseudomonas spp., (3), Achromobacter spp., Uncultured Microbacterium spp., and Exigoubacterium spp., which showing the growth up to the concentration of 2000 ppm. The potency of the six potential isolates was determined by using the conventional plate count technique.  The percentage removal of analyzed by the use of ICP-AES technique. The study shows isolation of the species which can remove heavy metal up to 60%. It was also found that the increase in the incubation time causes more reduction in the heavy metal concentration. The mutational analysis of the isolates for the strain improvement process shows that the Exigoubacterium species can grow at 3000 ppm heavy metal concentration and showed 60% reduction in heavy metal. This highly potential species can be used for the removal of different heavy metals which is also a viable, eco friendly and cost effective technology for cleanup of the environment. 

Profiling of essential mineral content, heavy metals, and bacterial contaminants in conventional and organic eggs available in the hypermarkets of the Eastern Province of Saudi Arabia

2021 ◽  
Vol 12 ◽  
Author(s):  
Saad Dahlawi ◽  
Mahmoud Berekaa ◽  
Khaled Salama ◽  
Ossama Labib ◽  
Saifullah ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Drinking Water ◽  
Saudi Arabia ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Plate Count ◽  
Eastern Province ◽  
Fecal Matter ◽  
Bacterial Contaminants

Background: Eggs are commonly regarded as one of the best sources of various macro, and micronutrients, in particular, high biological value (BV) proteins, and both saturated, and unsaturated fatty acids. Apart from proteins, eggs are excellent sources of various high health value lipid components, carotenoids, minerals, and both water and lipid-soluble vitamins. However, the nutrient-rich nature of this ‘superfood’ also makes them susceptible to microbial contamination from various sources, such as fecal matter, drinking water for layer chicken, and the surrounding environment. Moreover, drinking water and chicken feed are also sources of heavy metal contamination. The presence of both factors poses serious health concerns for consumers. Objective: The main aim of this research was to proximate heavy metals and bacterial contaminants in nine different brands of brown organic and white conventional eggs available in the hypermarkets of the Eastern Province of Saudi Arabia. Methods: An Inductively Coupled Plasma-Optical Emission Spectrometer (ICP-OES), was used to determine the concentration of metals with high sensitivity. Standard plate count eas followed by enrichment of bacterial contaminants in a double-strength nutrient broth medium for microbiological assessment. Results: The results indicated that Pb, Cd, Al, and As were present in high concentrations in all egg brands of both types (organic and conventional). The estimated daily intake (EDI) values for Ca, Fe, Mn, and Mg were within the range of WHO-recommended thresholds, and that these essential minerals were present in adequate amounts in all egg brands. The two major groups predominating the microbial loads were the family Enterobacteriaceae and Pseudomonas sp. Conclusion: The presence of heavy metals, such as Al, As, Pb, and Cd, in nearly all the brands, amounts that exceeded the established ADI and EDI thresholds were a considerable concern. In particular, the elevated levels of Cd and Pb, group 1 and group 2A probable human carcinogens respectively, demands that local regulatory authorities investigate the sources of heavy metal contamination, and alleviate this considerable risk to human health. Furthermore, this study could be a benchmark for establishing food safety and hygiene standards for local egg production, storage, handling, and transport in Saudi Arabia.

Assessment of Zinc, Iron, and Microbes concentrations in Blood Cockles (Anadara granosa) as Complementary Foods and Implications for reducing of Micronutrition Deficiency

2021 ◽  
pp. 3399-3403
Author(s):  
Margaretha Solang ◽  
Djuna Lamondo ◽  
Ramli Utina ◽  
Syam Kumaji ◽  
Zuliyanto Zakaria
Keyword(s):  
Heavy Metals ◽  
Plate Count ◽  
Food Control ◽  
National Agency ◽  
Filter Feeder ◽  
Total Plate Count ◽  
Zn Content ◽  
Blood Cockle ◽  
Anadara Granosa

Blood cockles (Anadara granosa) are filter feeder that can accumulate heavy metals and microbes so that need to pay attention to the maximum intake. The objective of this study was to evaluate the content of Fe, Zn, a total of microbes and maximum tolerable intake value (MTI). The study employed quantitative analysis. The samples were collected from Wonggarasi Timur Village, Pohuwato Regency, Gorontalo, Indonesia. The determination of Fe and Zn was carried out through Atomic Absorption Spectrophotometer and the microbes total was done by using the Total Plate Count test. Maximum Tolerable Intake value was calculated according to the provisions of JEFCA. The results of the study showed that the Fe and Zn average of blood cockles ranged from 227.85±5.04 ppm – 247.98±6.77ppm and 2.77±0.07ppm-2.82±0.09ppm. Microbes total ranged from 1.7 x 103 colonies/g – 2.4 x 103 colonies/g. The MTI value assumed for person weighing 70 kg based on Fe and Zn content of blood cockle is 0.226kg/day - 0.467kg/day and 24.822kg/day - 24.911kg/day. Blood cockles are a source of Fe, Zn and are safe for consumption according to MTI. Consumption of blood cockles helps reduce the problem of deficiency of Fe and Zn. The content of Fe is higher than the content of Zn in blood cockles. Blood cockles from Pohuwato regency are safe for consumption according to MTI. The microbes total in blood cockles ranged from 1.7x103 colonies/g -2, 4x 103 colonies/g. This total is below the Badan Pengawas Obat dan Makanan/ Indonesian National Agency of Drug and Food Control (BPOM) standard, which is 5 x 105 CFU/g so that the blood cockles are safe for consumption.

The Study of Conditions for Bioleaching Heavy Metals from Polluted Soil and Metabonomics in Penicillium chrysogenum

2021 ◽  
Vol 15 (1) ◽  
pp. 117-124
Author(s):  
Xinhui Deng ◽  
Ping Tan ◽  
Mengqi Ma ◽  
Liang Ma ◽  
Yunjun Yu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Organic Acids ◽  
Functional Groups ◽  
Penicillium Chrysogenum ◽  
Ph Value ◽  
Polluted Soil ◽  
Catabolic Pathways ◽  
Orthogonal Method ◽  
Lead And Zinc

The effects of the temperature, pH value and leaching time on the bioleaching ratio of heavy metals were studied using an orthogonal method in the paper. The metabonomics of Penicillium chrysogenum F1 were detected to deeply illustrate the bioleaching mechanism of Penicillium chrysogenumF1. The results showed that the bioleaching ratios of lead and zinc are the best at 30 °C on the 7th day, while those of cadmium and copper are the best at 20 °C on the 5th day. A pH value of 7.0 is best for heavy metal bioleaching ratios. The combination of 30 °C, pH 7.0 and 7 days is best for the total bioleaching ratios. The intensities of metabolites vary with the addition of heavy metal-polluted soil. The main catabolic pathways of glucose are not influenced by the heavy metal-polluted soil, though some metabolic enzymes are influenced, resulting in some metabolites undergoing upregulation or downregulation. Both organic acids and other metabolites containing functional groups (-NH2, -OH, -CHO, -CO) can extract heavy metals from soil.

scholarly journals Heavy metals and their bioavailability from soils in the long-term polluted Central Spiš region of SR

2009 ◽  
Vol 55 (No. 4) ◽  
pp. 167-172 ◽  
Author(s):  
P. Takáč ◽  
T. Szabová ◽  
Ľ. Kozáková ◽  
M. Benková
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Statistical Processing ◽  
Soil Samples ◽  
Acidic Ph ◽  
Mobile Form ◽  
Lead And Zinc ◽  
Mobile Forms ◽  
Metal Contents

The heavy metal contents and their bioavailability were monitored in soils of Central Spiš region of SR. This area belongs to long term contaminated and hygienically loaded areas. Soil contamination by heavy metals is caused especially by ore mining, processing and treatment. Soil samples from the Central Spiš contained higher proportions of mobile forms which corresponded to the following sequence Pb > Zn > Cu, while for mobilizable forms the sequence of investigated metals was as follows: Cu > Zn > Pb. Soil pH is one of the parameters that affect significantly the share of bioavailable forms of metals. Higher proportions of mobile fractions of metals were detected in samples taken from soils with acidic pH. Statistical processing confirmed a relationship between the percentage share of lead and zinc in the mobile form and the level of pH/KCl, which was significant for Zn: <I>r</I> = –0.53. The relationships for lead and copper were insignificant.

scholarly journals Karakteristik Kimia dan Mikrobiologi Makanan Ringan Khas Pemalang Ogel-ogel

2020 ◽  
Vol 9 (2) ◽  
pp. 44-49
Author(s):  
Siti Susanti ◽  
Fahmi Arifan ◽  
Murni Murni ◽  
Ana Silviana
Keyword(s):  
Heavy Metal ◽  
Salt Content ◽  
Rice Flour ◽  
National Standard ◽  
Plate Count ◽  
Total Plate Count ◽  
Glutinous Rice ◽  
Microbiological Characteristics ◽  
Snack Products

Penelitian ini bertujuan untuk mengetahui karakteristik kimia dan mikrobiologis sebagai dasar penentu kualitas produk makanan ringan ogel-ogel khas Pemalang agar sesuai dengan peraturan SNI 01-2886-2015. Ogel-ogel berbahan dasar tepung beras ketan yang dicampur dengan telur, keju, gula, dan garam. Selanjutnya adonan tersebut dicetak seperti ulat menggeliat dengan menggunakan ekstruder yang kemudian digoreng. Penelitian dilakukan secara deskriptif dengan parameter uji karakteristik kimia yaitu kadar karbohidrat, protein, lemak, air, abu, garam, logam berat, dan uji ketengikan serta mikrobiologi. Hasil penelitian menunjukkan bahwa ogel-ogel memiliki nilai ketengikan (nilai TBA) 2,16 mg MA/kg; kadar karbohidrat, protein, lemak, air, abu, dan garam masing-masing sebesar 44,75; 13,52; 39,42; 2,51; 0,08; dan 1,87%. Tidak ditemukan adanya kandungan logam berat pada ogel-ogel kecuali timah (10,56 mg/kg) dengan jumlah total mikroba sebanyak 0,87x104 CFU/g. Dapat disimpulkan bahwa secara umum mutu ogel-ogel ditinjau dari karakteristik mikrobiologi dan kimianya telah sesuai dengan Standar Nasional Indonesia tentang makanan ringan ekstrusi (SNI 01-2886-2015) kecuali kadar lemaknya yang masih tinggi. Perlu dipikirkan teknik produksi ogel-ogel dimasa yang akan datang supaya kadar lemaknya lebih rendah sehingga mutunya sesuai dengan SNI. Chemical and Microbiological Characteristic of Ogel-ogel Pemalang Typical SnackAbstractThis study was aimed to get more insight the chemical and microbiological characteristics as a basis for determining the quality of ogel-ogel (snack products typical of Pemalang) to comply with SNI regulations 01-2886-2015. Ogel-ogel made from glutinous rice flour mixed with eggs, cheese, sugar and salt. That dough was molded like a stretched worm by using an extruder and then was fried. The study was conducted descriptively with chemical (carbohydrate, protein, fat, water, ash, salt, heavy metal, and rancidity test) and microbiology (total plate count-TPC) characteristic as the parameters. The results showed that ogel-ogel had a rancidity (TBA) value of 2.16 mg MA/kg; carbohydrate, protein, fat, moisture, ash and salt content of 44.75; 13.52; 39.42; 2.51; 0.08; and 1.87% respectively. No heavy metal content was found in ogel-ogel except lead (10.56 mg/kg) with a total amount of microbes of 0.87x104 CFU/g. It can be concluded that as general, the quality of ogel-ogel in the aspects of microbiological and chemical characteristics was in accordance with the Indonesian National Standard of extruded snacks (SNI 01-2886-2015) except for its high fat content. Further technique to produce ogel-ogel with low fat is necessary to be contemplated to fulfill the quality of ogel-ogel in accordance with SNI.

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