Neuroprotective effects of proanthocyanidins of grape seed extracts against oxidative stress and apoptosis induced by 6-hydroxydopamine in PC12 cells

A doença de Parkinson (DP) é um distúrbio neurológico caracterizado pela destruição neuronal dopaminérgica. Devido à óbvia existência de ácidos graxos poliinsaturados (PUFA) principalmente em sementes de uva, Vitis Vinifera L era mais comumente usado em doenças cardiovasculares. O objetivo deste estudo foi investigar o papel das proantocianidinas do extrato de semente de uva exibindo efeitos neurocitoprotetores contra a citotoxicidade induzida por 6-hidroxidopamina (6-OHDA) em células PC12, prevenindo a depleção do conteúdo de GSH e reduzindo os níveis de nitrito e malondialdeído. As células foram pré-tratadas com proantocianidinas (100 µg / ml) e subsequentemente expostas a 6-OHDA a 50% da concentração letal. Nossos resultados demonstraram que a resposta ao PA em células PC12 aumentou significativamente a viabilidade celular, diminuiu a citotoxicidade. A atividade de apoptose induzida por OHDA foi determinada por citometria de fluxo usando anexina v e caspase-3 clivada e expressão de caspase-7 foram analisadas por western blot. Além disso, medimos marcadores de estresse oxidativo, como; Níveis de MDA, GSH e nitrito em células PC12. As proantocianidinas preveniram a citotoxicidade induzida por 6-OHDA, preveniram a depleção do conteúdo de GSH e reduziram os níveis de nitrito e malondialdeído. Além disso, as proantocianidinas atenuaram a redução induzida por 6-OHDA das proteínas caspase-3 e caspase-7 clivadas. Esses resultados sugerem que as proantocianidinas protegem as células PC12 contra a neurotoxicidade induzida pela 6-OHDA por meio da atividade antioxidante e apoptótica.

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Neuroprotective effects of proanthocyanidins of grape seed extracts against oxidative stress and apoptosis induced by 6-hydroxydopamine in PC12 cells

Conjecturas ◽

10.53660/conj-077-109-2 ◽

2021 ◽

Vol 21 (2) ◽

pp. 68-86

Author(s):

Patricia de Araújo Rodrigues ◽

Selene Maia de Morais ◽

Juliana Fernandes Pereira ◽

Albert Layo Costa de Assis ◽

Amanda Aragão Alves ◽

...

Keyword(s):

Oxidative Stress ◽

Western Blot ◽

Caspase 3 ◽

Grape Seed ◽

Vitis Vinifera L ◽

Neuroprotective Effects ◽

Caspase 7 ◽

6 Hydroxydopamine ◽

Grape Seed Extracts ◽

Seed Extracts

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Monitoring of compositional changes during berry ripening in grape seed extracts of cv. Sangiovese (Vitis vinifera L.)

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.8151 ◽

2017 ◽

Vol 97 (9) ◽

pp. 3058-3064 ◽

Cited By ~ 1

Author(s):

Giuseppe Bombai ◽

Federica Pasini ◽

Vito Verardo ◽

Onur Sevindik ◽

Michele Di Foggia ◽

...

Keyword(s):

Vitis Vinifera ◽

Grape Seed ◽

Vitis Vinifera L ◽

Berry Ripening ◽

Compositional Changes ◽

Grape Seed Extracts ◽

Seed Extracts

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Concentration effects of grape seed extracts in anti-oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-015-0621-8 ◽

2015 ◽

Vol 15 (1) ◽

Cited By ~ 28

Author(s):

Ching-Yu Yen ◽

Ming-Feng Hou ◽

Zhi-Wen Yang ◽

Jen-Yang Tang ◽

Kun-Tzu Li ◽

...

Keyword(s):

Oxidative Stress ◽

Dna Damage ◽

Oral Cancer ◽

Cancer Cells ◽

Grape Seed ◽

Concentration Effects ◽

Oral Cancer Cells ◽

Grape Seed Extracts ◽

Seed Extracts

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Neuroprotective Effects of Extracts from the Radix Curcuma aromatica on H2O2-induced Damage in PC12 Cells

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207321666181005121457 ◽

2018 ◽

Vol 21 (8) ◽

pp. 571-582 ◽

Cited By ~ 1

Author(s):

Juxiang Liu ◽

Lianli Zhang ◽

Dan Liu ◽

Baocai Li ◽

Mi Zhang

Keyword(s):

Oxidative Stress ◽

Pc12 Cells ◽

Caspase 3 ◽

Cell Damage ◽

Positive Control ◽

Neuroprotective Activity ◽

Antioxidant Enzyme Activities ◽

Morphologic Change ◽

Neuroprotective Effects ◽

Etoh Extract

Aim & Objectives: Curcuminoids are characteristic constituents in Curcuma, displaying obviously neuroprotective activities against oxidative stress. As one of the Traditional Chinese Medicines from Curcuma, the radix of Curcuma aromatica is also rich in those chemicals, but its neuroprotective activity and mechanism remain unknown. The aim of the current study is to evaluate the neuroprotective effects of extracts from the radix of C. aromatica (ECAs) on H2O2-damaged PC12 cells. Material and Methods: The model of oxidative stress damage was established by treatment of 400 µM H2O2 on PC12 to induce cell damage. After the treatment of ECWs for 24 h, the cell viability, LDH, SOD, CAT and GSH were measured to evaluate the neuroprotection of ECAs on that model. The potential action mechanism was studied by measurement of level of ROS, cell apoptosis rate, mitochondrial membrane potential (MMP), morphologic change, the intracellular Ca2+ content (F340/F380) and the expressions of Bcl-2, Bax and Caspase-3. Additionally, the constituents from tested extracts were analyzed by HPLC-DAD-Q-TOF-MS method. Results: Compared with a positive control, Vitamin E, 10 µg/ml of 95% EtOH extract (HCECA) and 75% EtOH extract (MCECA) can markedly increase the rate of cell survival and enhance the antioxidant enzyme activities of SOD, CAT, increase the levels of GSH, decrease LDH release and the level of ROS, attenuate the intracellular Ca2+ overloading, reduce the cell apoptotic rate and stabilize MMP, down-regulate Bcl-2 expression, up-regulate Bax and caspase-3 expression, and improve the change of cell morphology. The chemical analysis showed that diarylheptanoids and sesquiterpenoids are the major chemicals in tested extracts and the former were richer in HCECA and MCECA than others. Conclusions: These findings indicated that the effects of HCECA and MCECA on inhibiting the cells damage induced by H2O2 in PC12 are better than other extracts from the radix of C. aromatica, and the active constituents with neuroprotective effects consisting in those two active extracts are diarylheptanoids.

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Graphene oxide/polyethylene glycol aerogel reinforced with grape seed extracts as wound dressing

Journal of Materials Science ◽

10.1007/s10853-021-06297-z ◽

2021 ◽

Author(s):

Jessica Borges-Vilches ◽

Javiera Poblete ◽

Fernando Gajardo ◽

Claudio Aguayo ◽

Katherina Fernández

Keyword(s):

Graphene Oxide ◽

Polyethylene Glycol ◽

Wound Dressing ◽

Grape Seed ◽

Grape Seed Extracts ◽

Seed Extracts

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Artemisinin attenuated oxidative stress and apoptosis by inhibiting autophagy in MPP+-treated SH-SY5Y cells

Journal of Biological Research-Thessaloniki ◽

10.1186/s40709-021-00137-6 ◽

2021 ◽

Vol 28 (1) ◽

Author(s):

Junqiang Yan ◽

Hongxia Ma ◽

Xiaoyi Lai ◽

Jiannan Wu ◽

Anran Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Membrane Potential ◽

Protein Expression ◽

Mitochondrial Membrane Potential ◽

Western Blotting ◽

Cell Apoptosis ◽

Mitochondrial Membrane ◽

Caspase 3 ◽

Neuroprotective Effect ◽

Neuroprotective Effects

Abstract Background Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer's disease. The oxidative stress is an important component of the pathogenesis of PD. Artemisinin (ART) has antioxidant and neuroprotective effects. The purpose of this study is to explore the neuroprotective effect of ART on 1-methyl-4-phenyliodine iodide (MPP +)-treated SH-SY5Y cells and underlying mechanism. Methods We used MPP+-treated SH-SY5Y cells to study the neuroprotective effect of ART. Cell viability was measured by MTT assay after incubating the cells with MPP+ and/or ART for 24 h. DCFH-DA was used to detect the level of intracellular reactive oxygen species (ROS), and WST-8 was used to detect the level of superoxide dismutase (SOD). The level of intracellular reduced glutathione (GSH) was detected with 5,5΄-dithiobis-(2-nitrobenzoic acid), and the level of malondialdehyde (MDA) was assessed based on the reaction of MDA and thiobarbituric acid. A mitochondrial membrane potential detection kit (JC-1) was used to detect changes in the mitochondrial membrane potential (MMP), and an Annexin V-FITC cell apoptosis kit was used to detect cell apoptosis. The expression levels of caspase-3, cleaved caspase-3 and the autophagy-related proteins LC3, beclin-1, and p62 were detected by Western blotting. In addition, to verify the change in autophagy, we used immunofluorescence to detect the expression of LC3 and p62. Results No significant cytotoxicity was observed at ART concentrations up to 40 μM. ART could significantly increase the viability of SH-SY5Y cells treated with MPP+ and reduce oxidative stress damage and apoptosis. In addition, the Western blotting and immunofluorescence results showed that MPP+ treatment could increase the protein expression of beclin1 and LC3II/LC3I and decrease the protein expression of p62, indicating that MPP+ treatment could induce autophagy. Simultaneous treatment with ART and MPP+ could decrease the protein expression of beclin1 and LC3II/LC3I and increase the protein expression of p62, indicating that ART could decrease the level of autophagy induced by MPP+. Conclusion Our results indicate that ART has a protective effect on MPP+-treated SH-SY5Y cells by the antioxidant, antiapoptotic activities and inhibition of autophagy. Our findings may provide new hope for the prevention and treatment of PD.

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