scholarly journals Phytochemical compositions, antimicrobial activities, and thin layer chromatography analysis of aqueous, and methanol extracts of Tectona grandis leaf

2021 ◽  
Vol 01 (01) ◽  
pp. 44-51
Author(s):  
Hadiza Muhammed ◽  
Lare H. Abdullahi ◽  
Fatima G. Mayaki ◽  
Usman H. Boko
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Microbial Activity ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Tectona Grandis ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Chromatography Analysis ◽  
Layer Chromatography

Background: Teak (Tectona grandis) from the family Verbenaceae/Lamiaceae is indigenous to India, other tropical countries, and one of the naturally discovered plants to be known by scientists due to its high potential and effectiveness in disease preventive and curative action. The present study evaluated the phytochemical composition, anti-microbial activity of methanol and aqueous extract of Tectona grandis leaf, and characterized the extracts using thin-layer chromatography (TLC). Methods: The phytochemical, anti-microbial activity thin layer chromatography (TLC) analysis was conducted using established protocols. Results: The results revealed that the methanol extract of teak plant leaves contains steroids, tannins, saponin, coumarin, protein, carbohydrates, alkaloids, diterpenes, phytosterol, phlobatannin while the aqueous extract contains tannins, saponin, coumarin, protein, carbohydrate, alkaloid, diterpenes, phytosterol, and phlobatannins. The methanol extract of Tectona grandis showed the highest activity on S. epidermasis (14mm) and against S.aureus (10mm) at a concentration of 40mg/ml followed by the activities of aqueous extract of teak plant leaf against Candida Albicans (8mm) at a concentration of 40mg/ml. The extract had no inhibitory effect at all other concentrations. Conclusion: The result confirmed that Tectona grandis contain several bioactive phytochemicals that can be explored for the treatment of pathogenic microorganism.

scholarly journals ANTIMICROBIAL ACTIVITIES OF Shorea foxworthyi Sym STEAM BARK METHANOL EXTRACT

2010 ◽  
Vol 8 (1) ◽  
pp. 114-118
Author(s):  
Andi Hairil Alimuddin ◽  
Masriani Masriani
Keyword(s):  
Antimicrobial Activity ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Chloroform Extract ◽  
E Coli ◽  
Layer Chromatography

Screening of antimicrobial activity compound from steam bark of Shorea foxworthyi Sym by Thin Layer Chromatography-Bioautography method have been conducted. The result of this research can be base in elucidation of antimicrobial activity compounds from S. foxworthyi Sym. The first step was done in this research that is maceration of S. foxworthyi steam bark using methanol solvent. Fractination to methanol extract was done using n-hexane, chloroform, and ethyl acetate solvent, respectively. Phytochemical screening were done to methanol, chloroform, n-hexane, and ethyl acetate fractions. Screening of antimicrobial activity compound were done to polar fraction such as methanol, chloroform, and ethyl acetate fraction. The bacteria were used in this screening such as E. coli, S. aureus, S. thypii, and B. Subtilis. The extract was highest antimicrobial activity choosed to test by Thin Layer Chromatography-Bioautography. The result was showed that chloroform extract was had highest antimicrobial activity and the flavonoide of compaund was considered having antimicrobial activity.   Keywords: antimicrobial, TLC-Bioautography, and Shorea foxworthyi Sym

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA KIMIA SERTA UJI AKTIVITAS ANTICANDIDAISIS SERBUK BATANG SIRIH DUDUK (Piper sarmentosum Roxb. Ex Hunter)

Molekul ◽  
2007 ◽  
Vol 2 (2) ◽  
pp. 53
Author(s):  
Suwandri Suwandri ◽  
Hartiwi Diastuti ◽  
Purwati Purwati
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Traditional Medicine ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Gas Chromatography Mass Spectrometry ◽  
The People ◽  
Layer Chromatography

Sirih duduk or Piper sarmentosum Roxb. ex Hunter have been long used the people in Indonesia for traditional medicine to cure various diseases. One of them was used to treat fungoid diseases. This research was aimed to examine the anticandidaisis activity from extract of P. sarmentosum bark, isolate and identify anticandidaisis compounds from P. sarmentosum bark. The isolation of bioactive compounds of P. sarmentosum bark was performed by extraction the powder of P. sarmentosum bark using methanol. The methanol extract was then fractionated using n-hexane, chloroform and ethylasetat. The fractions respectively was examined their activity against C. albican. The active extract was then fractionated performed by coloumn chromatography and was then separated performed by thin layer chromatography. Identification of bioactive compound was carry out using gas chromatography-mass spectrometry (GC-MS). The results show that chloroform, ethylasetat and methanol extracts of P. sarmentosum have more activities against C. albicans than miconazol. Bioactive compound from extract of P. sarmentosum bark was bis(2-ethylhexyl)phtalat.

scholarly journals Preliminary Phytochemical Screening and Thin Layer Chromatography Analysis of Stem Bark Extracts of African Mistletoe Parasitic on Vitellaria paradoxa, Piliostigma thonningii and Combretum fragrans

2019 ◽  
pp. 1-6
Author(s):  
T. Agber Cyprian ◽  
Shaakaa Sewuese ◽  
Linus U. Akacha
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Heart Diseases ◽  
Stem Bark ◽  
Phytochemical Screening ◽  
Vitellaria Paradoxa ◽  
Chromatography Analysis ◽  
Plant Hosts ◽  
Layer Chromatography

Aim: Mistletoes are highly utilized in traditional medicine to treat different kinds of diseases such as heart diseases, diabetes and malaria, among others. The chemistry of African mistletoe is not sufficiently documented. This paper is therefore, aimed at determining the phytochemicals present in the crude extracts of mistletoe parasitic on plants that are commonly seen as hosts. Study Design: This study was designed to compare the phytochemical profiles of mistletoe stem barks obtained from different plant hosts. Place and Duration of Study: Department of Chemistry, University of Agriculture, Makurdi, Benue State Nigeria, between August and September, 2018.   Methodology: Powdered stem bark of mistletoe was extracted successively with hexane, ethyl acetate and methanol. Preliminary phytochemical screening was carried out on the extracts. Thin layer chromatography (TLC) was carried out on silica gel precoated plates in 9:1 (hexane/ethyl acetate), 1:1 (hexane/ethyl acetate), and 7:3 (ethyl acetate/methanol) mobile phases for hexane, ethyl acetate and methanol extracts respectively. Results: The study revealed the presence of secondary metabolites such as alkaloids, flavonoids, tannins/phenols, cardiac glycosides, steroids and triterpenoids. It was evident from TLC analysis that mistletoes from various plant hosts contain similar chemical profile. Conclusion: We therefore debunk the claim by some herbalists that medicinal values of mistletoes vary due to host plant. This is the first time a study of this kind is reported on mistletoe parasitic on Vitellaria paradoxa Pilostigma thonningii, Combretum fragrans.

Thin layer chromatography analysis of antioxidant constituents of lichens from Antarctica

2008 ◽  
Vol 62 (4) ◽  
pp. 481-484 ◽  
Author(s):  
Hari Datta Bhattarai ◽  
Babita Paudel ◽  
Soon Gyu Hong ◽  
Hong Kum Lee ◽  
Joung Han Yim
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Layer Chromatography
Sign in / Sign up

Export Citation Format

Share Document