scholarly journals Effect of Daily Egg Ingestion with Thai Food on Serum Lipids in Hyperlipidemic Adults

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Supanee Putadechakum ◽  
Pariya Phanachet ◽  
Varapat Pakpeankitwattana ◽  
Theerawut Klangjareonchai ◽  
Chulaporn Roongpisuthipong
Keyword(s):  
Washout Period ◽  
Serum Lipids ◽  
Crossover Trial ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Limited Data ◽  
Medication Treatment ◽  
Egg Consumption ◽  
Low Fat Diet ◽  
Randomized Crossover Trial

Thai food is one of the healthiest foods. In fact, several Thai dishes, such as Tom Yum soup, are currently under scientific study for their incredible health benefits. Limited data are available on the effects of egg consumption with Thai food in hyperlipidemic patients. To assess the effects of daily egg consumption with Thai food, which is known as low fat diet, on serum lipids profiles in hyperlipidemic subjects without medication treatment, the randomized crossover trial of 71 hyperlipidemic adults (8 men, 63 women) were randomly to one of the two sequences of one and three eggs/day for 4 weeks. Each treatment was separated by a four-week washout period (egg-free). Our data indicated that one or three eggs/day consumption were significantly increases total serum cholesterol ( and versus  mg/dL) and LDL-C levels ( and versus  mg/dL) as compared to egg-free period. No significant change of serum TG, HDL-C, TC/HDL-C, and LDL-C/HDL-C levels was observed after 1 or 3 eggs consumption daily in this study.

scholarly journals Effects of Single Oral Doses of Gemifloxacin (320 Milligrams) versus Trovafloxacin (200 Milligrams) on Fecal Flora in Healthy Volunteers

2001 ◽  
Vol 45 (2) ◽  
pp. 608-611 ◽  
Author(s):  
Gloria Garcia-Calvo ◽  
Ana Molleja ◽  
Maria J. Giménez ◽  
Araceli Parra ◽  
Eva Nieto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Healthy Volunteers ◽  
Washout Period ◽  
Crossover Trial ◽  
Fecal Flora ◽  
Randomized Crossover Trial ◽  
The Family ◽  
Stool Samples ◽  
Resistant Strains ◽  
Oral Doses

ABSTRACT Gemifloxacin and trovafloxacin were administered to 12 volunteers in a randomized crossover trial with a 2-week washout period. Stool samples were collected predose and 1, 2, and 3 days postdose. Both quinolones reduced the number of organisms of the familyEnterobacteriaceae and aerobic gram-positive organisms. Escherichia coli reduction was greater with gemifloxacin than with trovafloxacin, with postdose isolation of quinolone-resistant strains for which MICs of trovafloxacin were higher than those of gemifloxacin.

scholarly journals Effect of poloxamer 407 administration on the serum lipids profile, anxiety level and protease activity in the heart and liver of mice

2013 ◽  
Vol 6 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Tatyana Korolenko ◽  
Thomas P. Johnston ◽  
Nina I. Dubrovina ◽  
Yana A. Kisarova ◽  
Svetlana Ya. Zhanaeva ◽  
...  
Keyword(s):  
Serum Lipids ◽  
Morphological Changes ◽  
Cathepsin L ◽  
Chronic Administration ◽  
Cysteine Proteases ◽  
Heart Tissue ◽  
Poloxamer 407 ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Early Stages

Abstract Chronic administration of the poloxamer 407 (P-407), a block copolymer, to elevate serum lipids in mice is a well-established mouse model of hyperlipidemia and atherosclerosis. We tested the hypothesis that the activity of several types of proteases in heart and liver tissue is changed in the early stages of atherosclerosis development. Additionally, we evaluated whether increased serum lipids would induce anxiety in mice, as determined by using a ‘plus-maze’ test. The mice were administered P-407 by intraperitoneal injection twice a week for one month. P-407 administration to mice resulted in a marked increase in total serum cholesterol, atherogenic non-HDLcholesterol, and especially in total triglycerides, and it also increased anxiety. Morphological changes observed in P-407-treated mice included contractile type changes in cardiomyocytes and foamy macrophages in liver. A significant increase of cysteine proteases cathepsin B and cathepsin L (at 24 h) and aspartate protease cathepsin D (at both 24 h and 5 days) was determined in heart tissue following P-407 administration. However, no changes were noted in heart matrix metalloproteinase activity. The activity of cysteine and aspartate proteases was significantly increased in liver at both 24 hours and 5 days after P-407 administration. In conclusion, administration of P-407 to mice for one month resulted in increased anxiety, and more importantly, there was an increase in the activity of heart and liver proteases secondary to sustained dyslipidemia. It is suggested that heart and liver cysteine and aspartate proteases may represent potential therapeutic targets in the early stages of atherosclerosis.

scholarly journals Alterations in mood after changing to a low-fat diet

1998 ◽  
Vol 79 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Anita S. Wells ◽  
Nicholas W. Read ◽  
Jonathan D. E. Laugharne ◽  
N. S. Ahluwalia
Keyword(s):  
Dietary Fat ◽  
Plasma Cholesterol ◽  
Intervention Group ◽  
Hdl Cholesterol ◽  
Diet Group ◽  
Total Serum ◽  
Control Group ◽  
Total Serum Cholesterol ◽  
Low Fat ◽  
Low Fat Diet

The effects on mood of reducing dietary fat while keeping the energy constant were examined in ten male and ten female healthy volunteers aged between 20 and 37 years. Each volunteer consumed a diet containing 41% energy as fat for 1 month. For the second month half of the subjects changed to a low-fat diet (25% energy from fat) and the remainder continued to eat the diet containing 41% energy from fat. Changes in mood and blood lipid concentrations were assessed before, during and at the end of the study. Profile of mood states (POMS) ratings of anger–hostility significantly increased in the intervention group after 1 month on the low-fat diet, while during the same period there was a slight decline in anger–hostility in the control subjects (group F 6.72; df 1,14; P = 0.021). Tension–anxiety ratings declined in the control group consuming the higher fat diet but did not change in the group consuming the low-fat diet (group F 6.34; df 1,14; P = 0.025). There was a decline in fasting concentrations of HDL-cholesterol after the low-fat diet and a small increase in subjects consuming the medium-fat diet (group F 4.96; df 1,12; P = 0.046), but no significant changes in concentrations of total serum cholesterol, LDL-cholesterol or triacylglycerol were observed. The results suggest that a change in dietary fat content from 41 to 25% energy may have adverse effects on mood. The alterations in mood appear to be unrelated to changes in fasting plasma cholesterol concentrations.

scholarly journals Biochemical, Anthropometric, and Physiological Responses to Carbohydrate-Restricted Diets Versus a Low-Fat Diet in Obese Adults: A Randomized Crossover Trial

2020 ◽  
Vol 23 (3) ◽  
pp. 206-214 ◽  
Author(s):  
Mayumi Petrisko ◽  
Rebecca Kloss ◽  
Patricia Bradley ◽  
Erika Birrenkott ◽  
Audrey Spindler ◽  
...  
Keyword(s):  
Crossover Trial ◽  
Physiological Responses ◽  
Low Fat ◽  
Obese Adults ◽  
Low Fat Diet ◽  
Randomized Crossover Trial

Do Contact Precautions Reduce the Incidence of Intensive Care Unit–Acquired Pseudomonas aeruginosa Infections? The DPCPYO (Detection and Contact Precautions for Patients With P. aeruginosa) Cluster-Randomized Crossover Trial

2020 ◽  
Author(s):  
Céline Slekovec ◽  
Jérôme Robert ◽  
Philippe Berthelot ◽  
Nathalie van der Mee-Marquet ◽  
Anne-Marie Rogues ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Intensive Care ◽  
Washout Period ◽  
Primary Outcome ◽  
Crossover Trial ◽  
Screening Program ◽  
Standard Precautions ◽  
Contact Precautions ◽  
Randomized Crossover Trial ◽  
Cluster Randomized

Abstract Background The issue of contact precautions as contributory factors for reducing Pseudomonas aeruginosa (Pa) infections in intensive care units (ICUs) remains questioned. We evaluated the impact of the addition of contact precautions to standard precautions for Pa-positive patients on incidence of ICU-acquired Pa infections. Methods In this multicenter, cluster-randomized crossover trial, 10 French ICUs were randomly assigned (1:1) to sequence 0–1 (6-month control period [CP]/3-month washout period/6-month intervention period [IP]) or sequence 1–0 (6-month IP/3-month washout period/6-month CP). A surveillance screening program for Pa was implemented. Competing-risks regression models were built with death and discharge without the occurrence of ICU-acquired Pa infection (the primary outcome) as competing events. Models were adjusted for within-ICU correlation and patient- and ICU-level covariates. The Simpson diversity index (SDI) and transmission index (TI) of Pa isolates were derived from pulsed-field gel electrophoresis typing. Results Within recruited ICUs, the cumulative incidence and incidence rate of ICU-acquired Pa infections were 3.38% (55/1625) versus 3.44% (57/1658) and 3.31 versus 3.52 per 1000 patient-days at risk during the CP and IP, respectively. Multivariable models indicated that the intervention did not significantly change the cumulative incidence (subdistribution hazard ratio, .91; 95% confidence interval [CI], .49–1.67; P = .76) or rate (cause-specific hazard ratio, 1.36; 95% CI, .71–2.63; P = .36) of the primary outcome. SDI and TI did not significantly differ between CP and IP. Conclusions The addition of contact precautions to standard precautions for Pa-positive patients with a surveillance screening program does not significantly reduce ICU-acquired Pa infections in non-outbreak situations. Cl inical Trials Registration. ISRCTN92710225; 19 June 2020 (retrospectively registered); ISRCTN registry (http://www.isrctn.com/); DPCPYO trial: http://www.isrctn.com/ISRCTN92710225.

Micromethod for Total Serum Cholesterol that Eliminates Interference by High Bilirubin Concentrations

1970 ◽  
Vol 16 (1) ◽  
pp. 18-19 ◽  
Author(s):  
William J Jordan ◽  
Edward C Knoblock
Keyword(s):  
Total Cholesterol ◽  
Serum Lipids ◽  
Room Temperature ◽  
Dextran Sulfate ◽  
Direct Method ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Lipoprotein Complex ◽  
Acid Reagent ◽  
High Bilirubin

Abstract The analysis of pediatric serum for total cholesterol in the presence of high amounts of bilirubin presents special problems. The serum specimens are so small that a direct micro- or ultramicromethod has to be used, and consequently the error owing to bilirubin is impressive. In the proposed method, cholesterol is precipitated, along with other serum lipids, from only 20 µliters of serum as a dextran sulfate—lipoprotein complex. The precipitate is dissolved in molar NaCl, treated with glacial acetic acid, and incubated for 5 min at 70°C. A ferric chloride—sulfuric acid reagent is then added and, after 20 min at room temperature, the color is read at 560 nm. Total cholesterol values obtained by the proposed method are not statistically different from those obtained by the direct method of Pearson et al. Bilirubin added to sera does not affect the results. Agreement was obtained with the stated cholesterol values for Versatol-A and Moni-trol II reference sera to which known amounts of cholesterol and bilirubin had been added by the manufacturer.

scholarly journals Effect of a Flaxseed Lignan Intervention on Circulating Bile Acids in a Randomized, Crossover Trial

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1576-1576
Author(s):  
Sandi Navarro ◽  
Lisa Levy ◽  
Timothy Randolph ◽  
Natalie Hong ◽  
Fausto Neto ◽  
...  
Keyword(s):  
Dietary Fat ◽  
Mixed Models ◽  
Serum Lipids ◽  
Crossover Trial ◽  
Microbial Metabolites ◽  
Cross Sectional ◽  
High Fiber ◽  
Funding Sources ◽  
Randomized Crossover Trial ◽  
Sectional Analysis

Abstract Objectives Health benefits of high-fiber foods may be attributed, in part, to microbial metabolites of plant compounds. Lignans and their microbial metabolites, the enterolignans [enterolactone (ENL) and enterodiol (END)], reduce serum lipids through a variety of mechanisms, including regulation of bile acid (BA) synthesis. BA, released into the gut lumen in response to dietary fat, undergo microbial metabolism to secondary (2°) BA, which have been positively associated with chronic disease, e.g., liver disease and colorectal cancer. Our aims were to evaluate the effects of a flaxseed lignan supplement on circulating BA and examine associations between enterolignans and 2° BA. Methods We conducted a randomized, crossover trial of a flaxseed lignan supplement (50 mg/d secoisolariciresinol diglucoside) compared to placebo in 46 healthy men and women (20-45 y). Each period lasted 60 days, separated by a 60-day washout period. Six primary and fourteen 2° BA species were measured in fasting plasma using LC-MS. ENL and END were measured in 24-h urines by GC-MS. Low- and high-ENL excreters were defined as below and above the median 24-h ENL excretion at the end of the flaxseed lignan intervention (23.4 µmol/24 h). Linear mixed models were used to a) test the effects of the intervention on individual BA concentrations, overall and stratified by low and high ENL excreters; and b) to cross-sectionally determine the association between plasma 2o BA and ENL and END. Results There was no significant effect of the flaxseed lignan intervention compared to placebo on BA concentrations overall, or by ENL-excreter status, after FDR adjustment. In the cross-sectional analysis, irrespective of treatment, six 2° BA were statistically significantly associated with ENL (FDR < 0.05), with two positive associations (isolithocholic and lithocholic acids), and four inverse associations (glycoursodeoxycholic, glycohyodeoxycholic, hyodeoxycholic, and muricholic acids). Conclusions The flaxseed lignan intervention and subsequent ENL production had no effect on plasma BA concentrations. However, the strong associations between ENL excretion and certain 2° BA concentrations suggests that the gut microbial communities capable of producing ENL may also play a role in 2° BA metabolism. Funding Sources NIH.

scholarly journals The association between TSH within the reference range and serum lipid concentrations in a population-based study. The HUNT Study

2007 ◽  
Vol 156 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Bjørn O Åsvold ◽  
Lars J Vatten ◽  
Tom I L Nilsen ◽  
Trine Bjøro
Keyword(s):  
Serum Lipids ◽  
Ldl Cholesterol ◽  
Reference Range ◽  
Age Groups ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Population Based ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Population Based Study

Objective: The association between TSH and serum lipids in people with no apparent thyroid disease is insufficiently understood. We have studied the association between normal thyroid function, defined as TSH within the reference range of a general population, and concentrations of serum lipids. Design: Cross-sectional, population-based study with 30 656 individuals without known thyroid disease. Methods: Using general linear models, we calculated mean concentrations of total serum cholesterol, low-density lipoprotein (LDL) cholesterol, non-high-density lipoprotein (HDL) cholesterol, HDL cholesterol and triglycerides across categories of TSH. Results: Within the reference range of TSH, there was a linear and significant (P for trend <0.001) increase in total serum cholesterol, LDL cholesterol, non-HDL cholesterol and triglycerides, and a linear decrease (P for trend <0.001) in HDL cholesterol with increasing TSH. Subgroup analyses showed statistically significant associations for all lipids in men above 50 years of age, and for triglycerides in all age groups. For women, associations were statistically significant in all age groups except for HDL cholesterol in women below 50 years of age. The associations with triglycerides and HDL cholesterol were stronger among overweight than normal weight individuals. Conclusions: Within the range of TSH that is considered clinically normal, we found that increasing level of TSH was associated with less favourable lipid concentrations. The association with serum lipids was linear across the entire reference range of TSH.

scholarly journals The effect of a low-fat diet on luteal-phase prolactin and oestradiol concentrations and erythrocyte phospholipids in normal premenopausal women

1989 ◽  
Vol 61 (3) ◽  
pp. 651-661 ◽  
Author(s):  
Christine M. Williams ◽  
Karen Maunder ◽  
D. Theale
Keyword(s):  
Fatty Acid ◽  
Dietary Fat ◽  
Luteal Phase ◽  
Premenopausal Women ◽  
Significant Decline ◽  
Total Serum ◽  
Total Serum Cholesterol ◽  
Low Fat ◽  
Low Fat Diet ◽  
Inositol Phospholipids

1. Fifteen normal premenopausal women followed a low-fat diet for a period of 2 months.2. Daily fat intake was reduced from 81 g on their customary diet to 36 g on the low-fat diet. A significant reduction in total energy intake and an increase in the dietary polyunsaturated fat: saturated fat ratio was also observed. There was a significant decline in body-weight, percentage body fat and total serum cholesterol, but no significant change in high-density-lipoprotein-cholesterol.3. Luteal-phase prolactin concentrations were not altered, but there was a significant decline in oestradiol concentrations on the low-fat diet. The proportion of erythrocyte inositol-phospholipid was increased on the low-fat diet and there was a significant increase in the 20:4ω6 (arachidonate) content of inositol-phospholipids. No significant changes in the content or fatty acid compositions of other phospholipid fractions were observed.4. Further investigation of the effect of level and type of dietary fat on the content and fatty acid composition of membrane inositol-phospholipids are required. Long-term studies investigating the response of both prolactin and oestradiol to dietary fat reduction are also warranted.

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