scholarly journals STUDIES ON EFFECT OF WEATHER ON TRADITIONAL PHOTOSENSITIVE PTB-L AND HIGH YIELDING JAYA VARIETIES BY CURVILINEAR TECHNIQUE AT PATTAMBI, KERALA

MAUSAM ◽  
2022 ◽  
Vol 45 (4) ◽  
pp. 351-360
Author(s):  
R. P. SAMUI ◽  
A. CHOWDHURY
Keyword(s):  
Mean Values ◽  
Rain Fall

A SlU d)' wa... cond ucted with tradition al photosensitive PTR-I an d hijrh )ield ina Jaya varieties ofnet' j'rown duri n~ kharifcrop season in sanlly loem soil under rai n(C'tJ co nd ition at Pauambt.Th e a nalysis was basedon curvili near tech nique an d seasonal rain fall. max imum an d minimum temperatures a nd briahl hou rs ofsunsh inehave been used.Th e stull )' enshit's determination of respo nse of yield to l1ilTercni ranees ofweather factor s. From (hefinal cu rves de te rm ine d in till' IInnl)'si s it is also ru ~...ibl e 10 e S li m~ te yield for diffe rent mean values of theweather factorsThe stud) revealed that ramfull is not that important for Ja)·. variet y. It abo does not nenl la l'Je amount of su nshineCOmpDrN to PTD-1. For both va riet ies. a lUu imum temperature of 29-C appears ideal for opt imum)it"ld  

Colorimetric Determination of Hydroxyproline as Measure of Collagen Content in Meat and Meat Products: NMKL Collaborative Study

1990 ◽  
Vol 73 (1) ◽  
pp. 54-57 ◽  
Author(s):  
Kurt Kolar
Keyword(s):  
Collaborative Study ◽  
Colorimetric Method ◽  
Meat Products ◽  
Acid Oxidation ◽  
Colorimetric Determination ◽  
Mean Values ◽  
Collaborative Studies ◽  
Freeze Dried ◽  
Analytical Result

Abstract A colorimetric method for the determination of hydroxyproline as a measure of collagen in meat and meat products has been collaboratively studied in 18 laboratories. The method includes hydrolysis with sulfuric acid, oxidation with chloramine- T, and formation of a reddish purple complex with 4- dimethylaminobenzaldehyde. Five frozen and 3 freeze-dried samples were tested, ranging in content from 0.11 to 0.88% and from 0.39 to 4.0% hydroxyproline, respectively. The mean values of 2 identical samples were 0.245 and 0.251 %. The average recovery from a spiked sample was 96.1 %. The hydroxyproline content of a known sample (a mixture of 2 samples in the ratio 5:2) was calculated to 1.42%, which agrees well with the analytical result, 1.40%. In comparison with other collaborative studies, based on the ISO analytical method, the repeatability and reproducibility of this method agree well with the other results. This method was accepted as an official NMKL method by all national Committees, and has been adopted official first action by AOAC as an NMKLAOAC method.

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

Effect of induced metabolic acidosis on intracellular pH, buffer capacity and contraction force of human skeletal muscle

1985 ◽  
Vol 69 (5) ◽  
pp. 505-510 ◽  
Author(s):  
E. Hultman ◽  
S. Del Canale ◽  
H. Sjöholm
Keyword(s):  
Buffer Capacity ◽  
Recovery Period ◽  
Mean Values ◽  
Muscle Ph ◽  
Before And After ◽  
Ph Buffer ◽  
Total Capacity ◽  
Ph Decrease ◽  
Muscle Biopsies

1. Five volunteers were studied before and after oral administration of NH4Cl (0.3 g/kg body wt.) given in order to create a moderate acidosis. 2. The quadriceps femoris muscles were stimulated electrically for 75 s and muscle biopsies for determination of pH and metabolite content were taken before, at the end of contraction and after 10 min in the recovery period. 3. Muscle pH at rest (mean 7.04) was not significantly decreased after acidification despite an extracellular pH decrease of 0.15 unit. 4. After contraction muscle pH was significantly lower after NH4Cl. Mean values before and after acidification were 6.70 and 6.54 respectively. 5. The buffer capacity calculated as the total capacity of the muscle to buffer H+ produced during the isometric contraction before and after NH4Cl ingestion was reduced from 68.6 sl to 54.5 sl. 6. The force produced by contracting muscle was significantly lower at the end of the contraction period after NH4Cl ingestion, 44.6% of initial compared with 55.4% without NH4Cl.

scholarly journals pH of exudate test in the physiological quality of crambe seeds

2016 ◽  
Vol 46 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Charline Zaratin Alves ◽  
Lennis Afraire Rodrigues ◽  
Carlos Henrique Queiroz Rego ◽  
Josué Bispo da Silva
Keyword(s):  
Pearson Correlation ◽  
Raw Material ◽  
Mean Values ◽  
Tukey Test ◽  
Physiological Quality ◽  
The Subject ◽  
The Mean ◽  
C 30

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.

The Determination of the Probabilistic Properties of Velocities and Accelerations in Kinematic Chains With Uncertainty

1989 ◽  
Author(s):  
S. J. Lee ◽  
B. J. Gilmore
Keyword(s):  
Effective Length ◽  
Radial Clearance ◽  
Probabilistic Design ◽  
Link Length ◽  
Mean Values ◽  
Kinematic Chains ◽  
Analysis Package ◽  
The Mean ◽  
Linkage Model

Abstract A probabilistic model and methods to determine the means and variances of the velocity and acceleration of stochastically-defined planar pin jointed kinematic chains are presented. The presented model considers the effect of tolerances on link length and radial clearance and uncertainty of pin location as a net effect on the link’s effective length. The determination of the mean values and variances of the output variables requires the calculation of sensitivities of secondary variables with respect to the random variables. It is shown that this computation is straightforward and can be accomplished by a conventional kinematic analysis package. Thus, the concepts of tolerance and clearance have been captured by the model and analysis. The only input data is the nominal linkage model and statistical information. The “effective link length” model is shown to be applicable to both analytical solution and Monte Carlo simulation. The results from both methods are compared. This paper solves the higher-order kinematics problem for the probabilistic design analysis of stochastically defined mechanisms.

scholarly journals Determination of ventilatory threshold based on rating of perceived exertion scale

2004 ◽  
Vol 132 (11-12) ◽  
pp. 409-413 ◽  
Author(s):  
Stanimir Stojiljkovic ◽  
Dejan Nesic ◽  
Sanja Mazic ◽  
Dejana Popovic ◽  
Dusan Mitrovic ◽  
...  
Keyword(s):  
Perceived Exertion ◽  
Ventilatory Threshold ◽  
Rating Of Perceived Exertion ◽  
Physically Active ◽  
Mean Values ◽  
Significant Difference ◽  
Continuous Test ◽  
Dependent Samples ◽  
Increasing Load

The objective of the study was to test the possibility of using the fixed value (12-13) of the Rating of Perceived scale (RPE scale), as a valid method for determination of ventilatory threshold (VT). The sample of the subjects included 32 physically active males (age: 22.3; TV: 180.5; TM: 75.5 kg; V02max: 57.1 mL/kg/min). During the continuous test of progressively increasing load on a treadmill, cardiorespiratory and other parameters were monitored using ECG and gas analyzer. Following the test, VT and V02max were determined. During the test, at each level, at the scale from 6 to 20, the subjects pointed the number that suited best their currently feeling of strain. The RPE threshold was defined as constant value of 12-13. Average values of ventilatory and RPE threshold were expressed by parameters that were monitored and then compared by using t-test for dependent samples. No significant difference was found between mean values of VT and RPE threshold, when they were expressed by relevant parameters: speed, load, heart rate, absolute and relative oxygen consumption. Fixed value (12-13) of RPE scale may be used to detect the exercise intensity that corresponds to ventilatory threshold.

Determination of Menthol in Cigarette Tobacco Filler

1967 ◽  
Vol 50 (4) ◽  
pp. 770-773
Author(s):  
Charles L Tucker ◽  
C L Ogg
Keyword(s):  
Chromatographic Method ◽  
Colorimetric Method ◽  
Cigarette Tobacco ◽  
Mean Values ◽  
Accuracy And Precision ◽  
Standard Deviations ◽  
Gas Chromatographic Method

Abstract A gas chromatographic and a colorimetric method for determining menthol in cigarette tobacco filler were studied collaboratively by 15 laboratories. No statistically significant differences were found in the precisions within or between laboratories. There were no significant differences between mean values for the two methods for any of the samples. Trends toward higher mean values for the colorimetric method and lower within-laboratory standard deviations for the gas chromatographic method were noted. Remarks by collaborators suggest that the accuracy and precision of the colorimetric method may be improved, and further studies are recommended.

X-Ray Spectroscopy in the Clinical Laboratory

1961 ◽  
Vol 7 (2) ◽  
pp. 115-129 ◽  
Author(s):  
Samuel Natelson ◽  
Bertrum Sheid
Keyword(s):  
Total Phosphorus ◽  
Whole Blood ◽  
Phosphorus Content ◽  
Clinical Laboratory ◽  
Total Iron ◽  
Mean Values ◽  
X Ray ◽  
Significant Difference ◽  
Wet Ashing

Abstract X-ray spectroscopy was applied to the determination of the total phosphorus content of serum and the iron content of whole blood (hemoglobin). Samples were placed on confined spots on paper, dried, then passed through the X-ray field. Concentration was then determined from the peaks as drawn on a recorder. A device is described that permits automatic assay of successive samples dried on paper without delays between readings. Samples are placed in the field and rapidly withdrawn at 30-sec. intervals, the response of the recorder serving as a measure of concentration. Results for total phosphorus in serum and total iron in whole blood were compared with those obtained by a wet ashing procedure. No significant difference with respect to both mean values and precision was observed except that total iron by the wet ashing procedure was more precise. However, the X-ray technic was adequate for clinical purposes. Hemoglobin levels calculated from whole-blood iron values demonstrated that bilirubin, red cell debris, leukocytes, and lipids interfered in the colorimetric but not in the X-ray procedure. A hemoglobinometer based on the X-ray spectroscopic technic may, therefore, yield results of greater clinical significance.

scholarly journals Interlaboratory Comparison for the Determination of Five Residual Organochlorine Pesticides in Ginseng Root Samples by Gas Chromatography

2007 ◽  
Vol 90 (4) ◽  
pp. 1133-1141 ◽  
Author(s):  
Mei-Fung Kong ◽  
Serena Chan ◽  
Yiu-Chung Wong ◽  
Siu-Kay Wong ◽  
Della Wai-Mei Sin
Keyword(s):  
Measurement Uncertainty ◽  
Organochlorine Pesticides ◽  
Interlaboratory Comparison ◽  
Analytical Data ◽  
Quality Criterion ◽  
Asia Pacific ◽  
Ginseng Root ◽  
Mean Values ◽  
Z Scores

Abstract An interlaboratory comparison study for the determination of 5 residual organochlorine pesticides (hexachlorobenzene and 4 hexachlorocyclohexane isomers) in ginseng root was performed. This program [Asia Pacific Laboratory Accreditation Cooperation (APLAC) T049] was the first of its kind for an herbal matrix and involved the participation of 70 laboratories from 26 countries worldwide. Consensus mean values were computed statistically from the reported results, which were eventually used to assess the performance of individual laboratories in terms of the z-scores. The distribution of analytical data was found to be widespread, with standard deviation ranging from 43.9 to 55.9%, and the result patterns obtained were similar to those residue pesticide programs using other matrixes. Although the estimation of measurement uncertainty is a crucial requirement for all quantitative tests for laboratories that meet the requirements of International Organization for Standardization/International Electrotechnical Commisssion (ISO/IEC) 17025, some laboratories in this program had difficulties and showed unfamiliarity with respect to that quality criterion. It was recommended that laboratories review and rectify the situation promptly so that they would have a better understanding of measurement uncertainty or the test service provided.

Colorimetric Method for Rapid Determination of Serum Arginase

1967 ◽  
Vol 13 (10) ◽  
pp. 900-908 ◽  
Author(s):  
Brigitta Mellerup
Keyword(s):  
Rapid Determination ◽  
Colorimetric Method ◽  
Normal Range ◽  
Clinical Routine ◽  
Mean Values ◽  
Enzymatic Formation ◽  
Significant Difference ◽  
The Mean ◽  
Normal Men

Abstract A method for the determination of serum arginase is given which combines the enzymatic formation of urea with the sensitive method of Coulombe (1) for measuring this substance. This procedure allows more accurate determinations in the normal range than do previous methods described and is convenient for clinical routine. Significant difference is found between the mean values of normal men and women, 3.9 units/L. for the former and 2.9 units/L. for the latter.

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