scholarly journals Growth and Haematological Response of Clarias gariepinus to Garlic (Allium sativum) Supplemented Diet

2018 ◽  
Vol 8 (1) ◽  
pp. 67 ◽  
Author(s):  
Abigail John Onomu

This study investigated the growth and haematological response of Clarias gariepinus to different concentration of garlic supplemented diet for a period of 12 weeks. This study was carried out in 2011 in the premises of Fisheries Department, Delta State University, Asaba, Nigeria. The experimental diet was formulated to contain 0% (control), 0.5%, 1% and 3% garlic. Fish were sorted into four groups based on the diet and were fed 3% live body weight. Diets which contain garlic did not show a significant effect (P = 0.06) on the weight of C. gariepinus. However, fish fed diets containing garlic had a higher (P <0.001) total length compared to the control. Nevertheless, fingerlings fed 0.5% concentration of garlic in diet exhibited greater growth in terms of weight and length compared to fingerlings in all other treatment groups and the control. At the end of the experiment, the total red blood cell count (RBC), white blood cell count (WBC), haemoglobin (Hb) and packed cell volume (PCV) of fish in the garlic supplemented groups were greater (P <0.05) compared to the values prior to the commencement of the experiment and to the control. However, catfish fed 0.5% garlic diet were observed to have a higher value of RBC, PCV and Hb than the other concentrations. Increment in plasma protein was noted in catfish fed garlic supplemented diet than the control. This study demonstrates that garlic supplemented feed stimulates increment in both total length and haematological indices of fish. Supplementation of fish feed with garlic is therefore of high benefit in aquaculture as it boosts the ability of C. gariepinus to resist diseases.

2012 ◽  
Vol 1 (2) ◽  
pp. 222 ◽  
Author(s):  
Agatha Arimiche Nwabueze

<p>The effect of different concentrations of garlic (<em>Allium sativum</em>) supplement in fish diet on growth and haematological parameters of <em>Clarias gariepinus</em> fingerlings was investigated. Fish were separated into four experimental groups of 0% (controls), 0.5%, 1.0% and 3% concentrations of garlic in diet and fed at 3% body weight per day in a renewal static bioassay system. Garlic supplemented diet did not have any significant effect on weight gain of <em>C. gariepinus</em> when compared to fish in the control diet. However, final fish weight was significantly (P&lt;0.05) higher than the initial fish weight in all concentrations of garlic in fish diet and in the controls. No significant (P&gt;0.05) difference in total length was observed in fish fed different concentrations of garlic at the end of 12 weeks. An increase in total length was observed in fish with garlic supplemented diet over fish in the control diet. Fish fed different concentrations of garlic in diet showed slight differences in total length but did not significantly (P&gt;0.05) differ from each other in total length. Fish in 0.5% garlic diet had higher weight gain and total length than fish in the rest of the treatment groups and the controls. Final white blood cell (WBC), red blood cell (RBC), packed cell volume (PCV), haemoglobin (Hb) were observed to be significantly (P&lt;0.05%) higher than the initial counts in the treatment groups as compared to the controls. RBC, PCV and Hb were however, significantly higher in fish fed 0.5% garlic supplemented diets than other concentrations. Plasma protein was observed to increase significantly (P&lt;0.05) only in fish fed 0.5% and 1.0% garlic in diet. This study has shown that 0.5% (0.5g/kg) garlic supplement in fish feeds elicited more increase in fish total length and volume of haematological parameters of <em>C.</em> <em>gariepinus.</em> Garlic inclusion in fish diet at 0.5% (0.5g/kg) concentration is<em> </em>therefore beneficial for use in aquaculture to enhance the disease resistant status of <em>C. gariepinus</em>.<em> </em></p>


Author(s):  
Grzegorz Kardas ◽  
Alicja Zielińska ◽  
Adam Pancer ◽  
Piotr Kuna ◽  
Michał Panek

The two main chronic obstructive diseases are asthma &ndash; affecting 1-18% of adult population &ndash; and chronic obstructive pulmonary disease (COPD) prevalent in up to 6% of adults. In both cases the treatment depends on diseases&rsquo; severity. In management of these conditions, spirometry and complete blood count are two major monitoring tests. Our aim was to compare blood morphology results and spirometry values between patients in groups of different treatment intensity in asthma and COPD. By measuring that, we expected to study whether asthma/COPD patients have a need of stepping-up their treatment steps. Methods A retrospective analysis of patients admitted in 2013-2019 to an outpatient pulmonology clinic in Ł&oacute;dź (Poland). Spirometry values, complete blood count and information on pharmacological treatment were obtained from archival data. Patients were assigned with disease severity according to present GINA/GOLD recommendations. The study included 125 patients &ndash; 47 with COPD (22 females) and 78 with asthma (57 females). Results Among patients with asthma, a positive correlation in white blood cell count (WBC) (r=0,236, p=0.038) and ascending GINA treatment steps was found. Significant negative correlations were shown between ascending GINA treatment steps and FEV1, FEV1%, FVC%, MEF50, MEF50, PEF%. In COPD patients, positive correlations between ascending GOLD treatment groups and white blood cell count, neutrophil count, basophil percentage, platelet count (r=0.346; 0.309; 0.321; 0.401 respectively) were found. Negative correlations were shown between ascending GOLD treatment groups and FEV1, FEV1%, FVC, FVC%, MEF50, MEF50%, PEF, PEF% (r=-0.732; -0.575; -0.705; -0.498; -0.632; -0.558; -0.688; -0.597 respectively). Conclusions Negative correlations between ascending GINA and GOLD treatment steps and spirometry values may suggest that asthma and COPD patients may benefit from stepping-up the treatment steps earlier. Potential advantages of that more intensive treatment needs to be examined in the future


Author(s):  
E. A. Ivon ◽  
F. O. Sanusi-Jadesola ◽  
N. E. Edu ◽  
C. O. Anyanwu ◽  
G. M. Ubi ◽  
...  

Background and Objectives: The study measured the impact of crude oil fractions and its derivatives like kerosene on the early life and sub-adults growth of African catfish, Clarias gariepinus in terms of mild, acute and chronic toxicity effect. It also examines the effect of indiscriminate spillage of crude oil derivatives on aquatic organisms.  Materials and Methods: Blood profiles such as Red Blood Cell count (RBC), White Blood Cell count (WBC), Blood Differentials, Hemoglobin, (HB), and Packed Cell Volume (PCV) of the treated fishes and control were estimated after exposure of the fish to the kerosene pollutant. Behavioral changes in fish exposed to8.0ml/l, 16.0ml/l, 25.0ml/l and 50.0ml/l of kerosene pollutant varied from erratic swimming, moribund movement, jumping and lack of balance. Results: At concentration 8.0ml/L, mean total mortality was observed within 72hours of exposure. The lethal concentration (LC50) was 8.0ml/L and highest mean mortality observed with 80% kerosene pollutant. PCV of the exposed fish was 26% when compared to the control 27%, HB was 8.9 gdl as against 9.0 gdl in the control. Total WBC was 640 x 109/L as against 1280 x 109/L in control. RBC reading was 2.1 compared to 5.3 mm3 of the control.  Lymphocyte was 80%, Neutrophil was 4% and monocyte 16% compared to 62%, 8% and 30% in control respectively. There was decrease in white blood cells counts for 8.0, 16.0, 25.0 and 50.0ml/L groups (p<0.05) compared to the control. Conclusion: The study concludes that it is necessary to ensure the safety of aquatic life forms especially fishes, by minimizing aquatic pollution with kerosene to sustain fish food quality, availability and security.


2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Afamdi Anene ◽  
Eme Okories Okorie ◽  
Malachy N. O. Ajima ◽  
Janet Onyemaonwu

Dietary supplementation with herbal extract in the diet of fish has been noted to increase aquaculture production through the improvement of immunity and other physiological dysfunctions related to fish health. A feeding trial was conducted to assess the impact of incorporating varied concentrations of turmeric powder (TP) on haematological and biochemical responses in Clarias gariepinus fingerlings. Five test diets were composed as T1 ((control) (basal diet + 0 % TP)), T2 (basal diet + 1 % TP), T3 (basal diet + 2 % TP), T4 (basal diet + 3 % TP) and T5 (basal diet + 4 % TP). Fish specimens were apportioned into five groups in triplicate. Significantly lower (P<0.05) red blood cell count was found in those fed control diet compared with the other inclusion. Highest values of white blood cell count were noticed in T5 when compared with the control. There were significant differences in haemoglobin between T1 and T5. Highest values of serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, glucose and cholesterol level were found in T2 when compared with the control. TP can be used as feed supplement in the diet of C. gariepinus without any adverse effect on physiological responses of the fish.


1992 ◽  
Vol 68 (05) ◽  
pp. 583-588 ◽  
Author(s):  
Annika Dotevall ◽  
Christina Rångemark ◽  
Elsa Eriksson ◽  
Jack Kutti ◽  
Hans Wadenvik ◽  
...  

SummarySmoking is a risk factor for the development of atherosclerotic cardiovascular disease, in men as well as in women. An increased urinary excretion of the thromboxane metabolite 2,3-dinor-thromboxane B2 (Tx-M) has been observed in smokers of both genders, suggesting that cigarette smoking may facilitate cardiovascular disease via an action on the platelets. The present study addressed the hypothesis that the increased Tx-M excretion in female smokers reflects a true facilitation of platelet reactivity in vivo, rather than an increased destruction of the platelets. In healthy female volunteers (aged 20–46 years, 18 smokers and 17 non-smokers) platelet life-span and indices of platelet activity were determined, together with plasma levels of plasminogen activator inhibitor-1 (PAI-1), fibrinogen, peripheral blood cell counts and hematocrit. The urinary excretion of Tx-M was higher in smokers than in non-smokers (361 vs. 204 pg/mg creatinine, respectively, p <0.05), while plasma and urinary β-thromboglobulin, plasma platelet factor 4, platelet mean life-span and platelet production rate did not differ between the groups. PAI-1 activity, white blood cell count and hematocrit were higher in smokers than in non-smokers (p <0.05). These data indicate that smoking facilitates platelet formation of thromboxane A2 without affecting platelet survival; i.e. it increases the activity of platelets without affecting their viability to a measurable extent. Such an increase in platelet activity, operating in parallel to a reduced fibrinolytic activity and a higher hematocrit and white blood cell count, may play an etiological role in smoking-induced cardiovascular disease in women.


2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.


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