scholarly journals Dietary Supplement of Tumeric (Curcuma longa) Powder: Impact on Haematological and Biochemical Responses in Clarias gariepinus (Burchell, 1822) Fingerlings

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Afamdi Anene ◽  
Eme Okories Okorie ◽  
Malachy N. O. Ajima ◽  
Janet Onyemaonwu
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Curcuma Longa ◽  
Control Diet ◽  
Clarias Gariepinus ◽  
Basal Diet ◽  
Herbal Extract ◽  
Blood Cell Count ◽  
Biochemical Responses ◽  
The Impact

Dietary supplementation with herbal extract in the diet of fish has been noted to increase aquaculture production through the improvement of immunity and other physiological dysfunctions related to fish health. A feeding trial was conducted to assess the impact of incorporating varied concentrations of turmeric powder (TP) on haematological and biochemical responses in Clarias gariepinus fingerlings. Five test diets were composed as T1 ((control) (basal diet + 0 % TP)), T2 (basal diet + 1 % TP), T3 (basal diet + 2 % TP), T4 (basal diet + 3 % TP) and T5 (basal diet + 4 % TP). Fish specimens were apportioned into five groups in triplicate. Significantly lower (P<0.05) red blood cell count was found in those fed control diet compared with the other inclusion. Highest values of white blood cell count were noticed in T5 when compared with the control. There were significant differences in haemoglobin between T1 and T5. Highest values of serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, glucose and cholesterol level were found in T2 when compared with the control. TP can be used as feed supplement in the diet of C. gariepinus without any adverse effect on physiological responses of the fish.

scholarly journals Measurement of Sub-Lethal Toxicity and Effect of Kerosene Pollutant on Hematological Profile of African Catfish (Clarias gariepinus)

2021 ◽  
pp. 1-17
Author(s):  
E. A. Ivon ◽  
F. O. Sanusi-Jadesola ◽  
N. E. Edu ◽  
C. O. Anyanwu ◽  
G. M. Ubi ◽  
...  
Keyword(s):  
Blood Cell ◽  
Crude Oil ◽  
Cell Count ◽  
Clarias Gariepinus ◽  
Total Mortality ◽  
African Catfish ◽  
Blood Cell Count ◽  
Fish Food ◽  
Aquatic Life ◽  
The Impact

Background and Objectives: The study measured the impact of crude oil fractions and its derivatives like kerosene on the early life and sub-adults growth of African catfish, Clarias gariepinus in terms of mild, acute and chronic toxicity effect. It also examines the effect of indiscriminate spillage of crude oil derivatives on aquatic organisms.  Materials and Methods: Blood profiles such as Red Blood Cell count (RBC), White Blood Cell count (WBC), Blood Differentials, Hemoglobin, (HB), and Packed Cell Volume (PCV) of the treated fishes and control were estimated after exposure of the fish to the kerosene pollutant. Behavioral changes in fish exposed to8.0ml/l, 16.0ml/l, 25.0ml/l and 50.0ml/l of kerosene pollutant varied from erratic swimming, moribund movement, jumping and lack of balance. Results: At concentration 8.0ml/L, mean total mortality was observed within 72hours of exposure. The lethal concentration (LC50) was 8.0ml/L and highest mean mortality observed with 80% kerosene pollutant. PCV of the exposed fish was 26% when compared to the control 27%, HB was 8.9 gdl as against 9.0 gdl in the control. Total WBC was 640 x 109/L as against 1280 x 109/L in control. RBC reading was 2.1 compared to 5.3 mm3 of the control.  Lymphocyte was 80%, Neutrophil was 4% and monocyte 16% compared to 62%, 8% and 30% in control respectively. There was decrease in white blood cells counts for 8.0, 16.0, 25.0 and 50.0ml/L groups (p<0.05) compared to the control. Conclusion: The study concludes that it is necessary to ensure the safety of aquatic life forms especially fishes, by minimizing aquatic pollution with kerosene to sustain fish food quality, availability and security.

scholarly journals Haematological and serum biochemical indices of broiler chickens fed graded levels of inorganic and chelated trace minerals

2020 ◽  
Vol 47 (2) ◽  
pp. 46-50
Author(s):  
A. A. Ayoola ◽  
A. O Fafiolu ◽  
O. O Oluwatosin ◽  
O. A Osinowo ◽  
O. W. Ariyo
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Broiler Chickens ◽  
Environmental Concern ◽  
Basal Diet ◽  
Serum Biochemistry ◽  
Trace Minerals ◽  
Blood Cell Count ◽  
Red Blood Cell Count ◽  
Biochemical Indices

Poor bioavailability of inorganic trace elements used in broiler nutrition and their subsequent release into the environment via poultry excereta is major cause of biological and environmental concern. Hence, the need to explore the use of Chelated trace minerals in broiler diets. This study was carried out to evaluate the effect of supplemental inorganic and chelated Cu, Zn and Mn on haematology and serum biochemistry of broiler chickens. A 42-day feeding trial was conducted using a total of 300 Arbor acre broiler chickens. The birds were allocated to five dietary groups. Each group had six replicates containing 10 birds. The treatments were control (basal diet), 100% inorganic trace minerals (ITMs) supplemental level (15, 100, 100 mg/kg for Cu, Zn, Mn respectively), 50% ITMs supplemental level (7.5, 50, 50 mg/kg of Cu, Zn, Mn), 50% Chelated trace minerals (CTMs) supplemental level (7.5,50, 50 mg/kg of Cu, Zn, Mn) and 25% CTM supplemental level (3.75, 25, 25 mg/kg of Cu, Zn, Mn, in that order). A lower (p<0.05) white blood cell count (11.07x106/mm3) and an improved (p<0.05) red blood cell count (13x106/mm3) was observed for the group fed 50% CTM supplemental level. The group fed 50% supplemental level of CTM had the highest (p<0.05) total protein (73.2 g/L), albumin (41.20 g/L) and globulin (32 g/L) in serum as compared tothe other groups. The serum Cu, Zn and Mn were significantly higher (p<0.05) for the grou fed 25% supplemental diet of CTM. It can be concluded that trace minerals supplementation increased serum trace minerals of broiler chicken.

scholarly journals P.109 Causes of albuminocytologic dissociation and the impact of an age-adjusted reference limit on review of 2,627 CSF samples

2018 ◽  
Vol 45 (s2) ◽  
pp. S45-S45
Author(s):  
JA Brooks ◽  
C McCudden ◽  
A Breiner ◽  
P Bourque
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Care Center ◽  
Tertiary Care ◽  
Common Finding ◽  
Blood Cell Count ◽  
Reference Limit ◽  
Number Of Patients ◽  
Upper Reference Limit ◽  
The Impact

Background: We set out to test the discriminative power of an age-adjusted upper reference limit (URL) for CSF total protein (CSF-TP) in identifying pathological causes of albuminocytologic dissociation (ACD). Methods: We reviewed the charts of 2,627 adult patients who underwent a lumbar puncture at a tertiary care center over a 20-year period. Samples with CSF-TP above 45 mg/dL (0.45 g/L) were included. Samples with white blood cell count > 5×109/L, red blood cell count > 50×109/L, and glucose < 2.5 mmol/L (45 mg/dL) were excluded. Patients with CSF-TP elevated above 45 mg/dL were considered to have ‘pseudo’ albuminocytologic dissociation (ACD) or ‘true’ ACD if their CSF-TP was in excess of age-adjusted norms. Results: Among all patients with ACD, a pathological source of CSF-TP elevation was identified in 57% (1490/2627) of cases, 51% of those with ‘pseudo’ ACD, and 75% with ‘true’ ACD (p< 0.001). Use of an age-adjusted upper reference limit favored the detection of polyneuropathy patients (13.5% proportionate increase) and excluded a larger number of patients with isolated headache (10.7% proportionate decrease; p < 0.0001). Conclusions: Elevated CSF-TP is a relatively common finding. Use of age-adjusted upper reference limits for CSF-TP values improve diagnostic specificity and help to avoid over-diagnosis of ACD.

The impact of preapheresis white blood cell count on autologous peripheral blood stem cell collection efficiency and HSC infusion side effect rate

2018 ◽  
Vol 33 (3) ◽  
pp. 331-341 ◽  
Author(s):  
Araci. M. Sakashita ◽  
Andrea T. Kondo ◽  
Ana Paula H. Yokoyama ◽  
Sanny M. C. Lira ◽  
Carolina B. Bub ◽  
...  
Keyword(s):  
Stem Cell ◽  
Blood Cell ◽  
Cell Count ◽  
Peripheral Blood ◽  
Side Effect ◽  
Peripheral Blood Stem Cell ◽  
Collection Efficiency ◽  
Blood Cell Count ◽  
The Impact ◽  
Stem Cell Collection

scholarly journals Digestive Fermentation, Antioxidant Status, and Haemato-Biochemical Indices of Growing Rabbits Fed on Diets Supplemented with Ziziphus spina-christi Leaf

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Suha Hashim Abduljawad
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Antioxidant Status ◽  
Control Diet ◽  
Organic Matter Content ◽  
Control Group ◽  
Chemical Compositions ◽  
Blood Cell Count ◽  
High Level ◽  
Biochemical Indices

The objective of the study was to assess the effect of diets supplemented with Ziziphus spina-christi leaf on digestive fermentation, antioxidant status, and haemato-biochemical indices of growing rabbits. Ziziphus leaves (ZLs) of Ziziphus spina-christi were collected from Sidr trees scattered throughout the city of Medina, Saudi Arabia. Three formulated diets containing 0, 10, and 20 g Ziziphus spina-christi/Kg diet as supplementation were offered ad libitum. The organic matter content of ZL was higher. Chemical compositions were comparable in all of the contents of the tested diet. Quantities of gas released from the control diet were higher, and then the gases released decreased significantly (P<0.05) with the addition of ZL. The values of NH3-N were taken as the same trend. The addition of a high level of ZL to rabbit diets led to a decrease (P<0.05) in the total count of bacteria as well as the number of E. coli and Clostridium spp. However, the number of Enterococcus bacteria was not affected by supplementation. Haemoglobin parameters of the control group and groups 2 and 3 were compared: white blood cell count and red blood cell count. These observations of total protein and albumin within the range of reference values were reported in healthy rabbits, while glucose significantly decreased with the addition of ZL and AST in the blood increased significantly. The values of TP, albumin, and ALT measurements showed no significant differences among groups fed on test diets. Significant differnces in serum immunoglobulins were observed between the groups, while the high levels of ZL supplement led to a significant (P<0.05) increase in the serum IgA, IgG, and IgM levels. Antioxidants expressed as T-AOC, GSH-Px, T-SOD, and CAT in the blood of animals fed on diets containing high levels of ZL were significantly higher. Higher serum T-AOC, T-SOD, and CAT activities were observed in rabbits supplemented with a high level of ZL compared with the control group (P<0.05). The supplementation of ZL tended to increase serum GSH-Px activity. The addition of ZL to rabbit diets led to an increase in dry matter intake. On the other hand, there was no significant change in the apparent digestion coefficient of DM, OM, CP, and fat. Conclusion. Dried ZL supplementation up to 20 g/Kg diet might improve the bacterial community, antioxidants, biochemical parameters and blood constituents of rabbits, and digestibility.

scholarly journals Utility of computed tomography and derivation and validation of a score to identify an emergent outcome in 2,315 patients with suspected urinary tract stone

CJEM ◽  
2013 ◽  
Vol 15 (05) ◽  
pp. 261-269 ◽  
Author(s):  
F. Kris Aubrey-Bassler ◽  
Scott D. Lee ◽  
Richard B. Barter ◽  
Shabnam Asghari ◽  
Richard Cullen ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Blood Cell ◽  
Cell Count ◽  
Ct Scans ◽  
Potential Range ◽  
Blood Cell Count ◽  
Computed Tomographic ◽  
Urinary Tract Stone ◽  
The Impact ◽  
Predicted Risk

ABSTRACT Objective: Because a majority of urinary tract stones (UTSs) pass spontaneously and clinically significant alternative pathology is rare, we hypothesize that many computed tomographic (CT) scans to diagnose them are likely unnecessary. We sought to measure the impact of renal CT scans on resource use and to justify a prospective study to derive a score that predicts an emergent diagnosis in patients with suspected UTS by doing so in our retrospective series. Methods: We conducted a retrospective study of ED patients who had noncontrast CT of the abdomen for suspected UTS. A split-sample was used to derive and validate a score to predict the presence of an emergent diagnosis on CT. Results: Of the 2,315 patients (50.8% female, mean age 45 years), 49 (2.1%) had an emergent outcome observed on CT. An additional 12 (0.5%) patients had an urgent outcome and 239 (10.6%) had a urologic procedure within 8 weeks of the CT. Serum white blood cell count, highest temperature, urine red blood cell count, and the presence of abdominal pain were significant predictors of the primary outcome. A score derived using these predictors had a potential range of 22 (0.26% predicted risk, 0.5% actual risk of the outcome) to 6 (52% predicted risk). The score was moderately discriminatory with c-statistics of 0.752 (derivation) and 0.668 (validation) and accurate with Hosmer-Lemeshow statistics of 10.553 (p = 0.228, derivation) and 9.70 (p = 0.286, validation). Conclusions: A sensible, relevant score derived and validated on all patients presenting with symptoms suggestive of renal colic could be useful in reducing abdominal CT scan ordering.

scholarly journals Mediterranean Diet and White Blood Cell Count—A Randomized Controlled Trial

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1268
Author(s):  
Álvaro Hernáez ◽  
Camille Lassale ◽  
Sara Castro-Barquero ◽  
Nancy Babio ◽  
Emilio Ros ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
White Blood Cell Count ◽  
White Blood Cell ◽  
Lower Risk ◽  
Control Diet ◽  
Incidence Rates ◽  
Blood Cell Count ◽  
High Cardiovascular Risk ◽  
All Cause Mortality

We aimed to assess the effects of the antioxidant-rich Mediterranean diet (MedDiet) on white blood cell count. Our study population included participants in the PREvención con DIeta MEDiterránea study (average age 67 years old, 58% women, high cardiovascular risk). We assessed whether a MedDiet intervention enriched in extra-virgin olive oil or nuts, versus a low-fat control diet, modified the incidence of leukocytosis (>11 × 109 leukocytes/L), mild leukopenia (<4.5 × 109 leukocytes/L), or severe leukopenia (<3.5 × 109 leukocytes/L) in individuals without the condition at baseline (n = 3190, n = 2925, and n = 3190, respectively). We also examined whether MedDiet modified the association between leukocyte count alterations and all-cause mortality. Both MedDiet interventions were associated with a lower risk of developing leukopenia (incidence rates: 5.06% in control diet, 3.29% in MedDiet groups combined; hazard ratio [95% confidence interval]: 0.54 [0.36–0.80]) and severe leukopenia (incidence rates: 1.26% in control diet, 0.46% in MedDiet groups combined; hazard ratio: 0.25 [0.10–0.60]). High cumulative adherence to a MedDiet was linked to lower risk of leukocytosis (incidence rates: 2.08% in quartile 1, 0.65% in quartile 4; HRQ4-Q1: 0.29 [0.085–0.99]) and attenuated the association between leukopenia and all-cause mortality (P-interaction = 0.032). In brief, MedDiet decreased the incidence of white blood cell count-related alterations in high cardiovascular risk individuals.

266 Growth Performance, Fecal Score, and Blood Immune Parameters of Nursery Pigs Challenged with Escherichia coli F18 Fed Canola Meal-based Diet

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 98-98
Author(s):  
Jinsu Hong ◽  
Samuel Ariyibi ◽  
Joy Scaria ◽  
Steven Dilberger-Lawson ◽  
David Francis ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Cell Count ◽  
Basal Diet ◽  
Canola Meal ◽  
Blood Cell Count ◽  
Blood Samples ◽  
Nursery Pigs ◽  
E Coli ◽  
Fecal Score

Abstract Effects of dietary canola meal (CM) on growth performance, fecal score, and blood parameters of E. coli-challenged nursery pigs were investigated. Thirty-six pigs (initial body weight = 6.22 kg) weaned at 21 d of age were housed individually and fed 3 diets (12 replicates/diet) in randomized complete block design. The diets were corn-soybean meal-based diet without or with antibiotics (0.2% chlortetracycline and 0.2% tiamulin) or with CM (20%). The diets were fed in 2 phases; Phase 1: d 0 to 7, and Phase 2: d 7 to 21. Pigs were orally challenged with F18 strain of E. coli on d 7. Feed intake and body weight were measured on d 7, 14, and 21. Blood samples for measuring thyroid hormones were collected from each pig on d 7, 14, and 21. Blood samples for measuring white blood cells, immunoglobulins, and cytokine were collected on d 21. Fecal score was assessed daily. Dietary antibiotics increased (P &lt; 0.05) ADG by 50%. Dietary SECM also increased (P &lt; 0.05) ADG by 23%. Week 1 fecal score was unaffected by diet. However, during weeks 2 and 3, fecal score for diet with antibiotics was less (P &lt; 0.05) than that for basal diet or diet with CM, which did not differ in fecal score. During weeks 1 and 2, serum tetraiodothyronine level for diet with antibiotics was less (P &lt; 0.05) than that for basal diet or diet with CM, which did not differ in serum tetraiodothyronine level; week 3 serum tetraiodothyronine level was unaffected by diet. White blood cell count was reduced (P &lt; 0.05) by dietary antibiotics, and tended to be reduced (P = 0.087) by dietary CM. In conclusion, dietary CM increased ADG and tended to reduce white blood cell count. Thus, dietary CM at 20% may improve the performance of pigs challenged with E. coli F18 partly by decreasing immune response.

scholarly journals 144 Effect of sodium butyrate (SB) on growth performance and complete blood cell count in nursery pigs: Two facility study

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 76-77
Author(s):  
Tsung Cheng Tsai ◽  
Hans H Stein ◽  
Casey L Bradley ◽  
Laia Blavi ◽  
Kristopher Bottoms ◽  
...  
Keyword(s):  
Blood Cell ◽  
Growth Performance ◽  
Cell Count ◽  
Sodium Butyrate ◽  
Control Diet ◽  
Phase 1 ◽  
Complete Blood Cell Count ◽  
Blood Cell Count ◽  
Eosinophil Cell ◽  
Dietary Treatments

Abstract A total of 344 weaned pigs (21 ± 2 d of age) were used at University of Arkansas (UA, n = 2 16) and University of Illinois at Urbana-Champaign (UIUC, n = 128) to evaluate increasing level of sodium butyrate (SB) on growth performance and complete blood cell count. Pigs at each facility were blocked by initial BW and randomly allotted to 1 of 4 dietary treatments with 9 replications/diet and 6 pigs/pen at UA; and 8 replications/diet and 4 pigs/pen at UIUC. Treatments included a control corn-soybean-meal based diet and 3 diets in which 0.05, 0.10, or 0.15% SB was added to the control diet. Feed was manufactured at each facility. Pigs were fed in 3 phases: 7 d, 14 d, and 14 d at UIUC and 7 d, 14 d, and 19 d at UA for phase 1, 2, and 3, respectively. At UA, blood was collected at the beginning of the experiment and at the end of each phase to determine complete blood cell count. Data for growth performance for both facilities were pooled and analysed as a RCBD using the Mixed procedure of SAS. Treatment was the fixed effect, and facility and facility by treatment interactions were random effects. Orthogonal contrasts were used to assess linear and quadratic responses to inclusion of increasing levels of SB in diets. Increasing dietary SB increased weight gain (quadratic, P < 0.05), ADFI (quadratic, P ≤ 0.05), and final BW (quadratic, P < 0.05). Total white blood cell and eosinophil cell count tended to increase with increasing SB (quadratic, P = 0.07 and P = 0.08, respectively). The lymphocyte cell count tended to decrease (linear, P = 0.09) with increasing SB. Results indicated that feeding SB during the nursery phase tended to alter blood cell count and improve growth performance.

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