МОНГОЛ ОРНЫ ЗАРИМ ЗҮЙЛ ЭМИЙН УРГАМЛЫН АНТИОКСИДАНТ ИДЭВХ

Сүүлийн жилүүдэд нийлэг аргаар гарган авсан хүнс болон эмийн бүтээгдэхүүнээс татгалзаж, аль болох байгалийн гаралтай бүтээгдэхүүн хэрэглэх хандлага єсєн нэмэгдэж байгаагийн зэрэгцээ эмийн ургамлын антиоксидант идэвхийн судалгаа ихээхэн сонирхол татсан сэдвийн нэг болоод байна.Бидний судалгааны үр дүнд Монголын ургамлын аймгийн Нийлмэл цэцэгтэн ба Сарнайн овгийн 23 зүйл ургамлын метанолын 51 ханднаас 20 зүйл ургамлын 28 ханд нь чєлєєт радикалыг дарангуйлах сайн идэвхтэй байсан ба ялангуяа ургамлын газрын дээд хэсэг цэцэг, навч, үрийн ханд илүү сайн идэвхтэй байв.Судалгааны дүнд Chamaerhodos erecta-н газрын дээд хэсэг 100µg/ ml концентрацдаа 81.33±0.96 %, Cotoneaster melanocarpa-н иш 100µg/ml концентрацдаа 92.14±0.98 %, Dasiphora fruticosa-н навч, цэцэг, иш 25µg/ml концентрацдаа тус бүр 49.61±4.4 %, 49.98±2.87 %, 57.7±0.84 %, Dasiphora parvifolia-н навч, цэцэг 25µg/ml концентрацдаа 43.22±1.4 %, Filipendula palmatа- н навч, үр 50µg/ml концентрацдаа тус бүр 56.4±0.75 %, 55.41±2.1 %, Rubus sachalinensis-н навч 50µg/ml концентрацдаа 65.22±0.91%-аар чєлєєт радикалыг дарангуйлах онцгой сайн идэвхтэй үр дүнтэй байлаа.Түлхүүр үг: Нийлмэл цэцэгтэн, Сарнайн овог, антиоксидант идэвх, DPPH радикал Screening of Mongolian Medicinal Plants for their Antioxidant Activity Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are implicated in a wide range of human diseases such as atherosclerosis, certain cancer, inflammation, diabetes, liver injury, Alzheimer, Parkinson and coronary heart pathologies [1, 11]. When an imbalance between ROS/RNS generation and antioxidants occurs, oxidative damage will spread over all the cell targets (DNA, lipids, proteins).Antioxidants in foods and medicinal plants have attracted interest in recent years.The aim of the present study was to search the antioxidant active compounds or a new plant species through the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method of the methanol extracts from 23 plant species belonging to 2 botanical families (Compositae, Rosaceae) collected from natural reserves. The antioxidant activity of these plant extracts were compared with standard solution of rutin.From the 51 plant extracts studied, twenty eight gave IC50 values lower than 200µg/ml. The strongest IC50 values were given by the methanol extracts of Chamaerhodos erecta (aerial parts), Dasiphora parvifolia (leaves, flowers), Cotoneaster melanocarpa (stems), Rubus sachalinensis (stems, leaves), Dasiphora fruticosa (flowers, leaves, stems), Cotoneaster melanocarpa (stems), Filipendula palmata (seeds, leaves), Rubus sachalinensis(leaves).Therefore, these plants may have great relevance in the prevention and therapies of diseases in which free radicals are implicated. In further, more detailed studies on the chemical composition of those extracts as well as studies with other models such as in vivo assays will be called for an interest.Acknowledgements We are grateful to prof A.H.Brantner (Laboratory of Pharmacognosy, University of Graz, Austria) for her helpful suggestion. This study was partially supported by Honda foundation, Japan.DOI: http://dx.doi.org/10.5564/pmas.v0i1.58 Proceedings of the Mongolian Academy of Sciences 2010 No.1 pp.48-56

Download Full-text

Antioxidant and Hypoglycemic Properties of Seeds of Cucurbitaceae Family from Western Nepal

Journal of Health and Allied Sciences ◽

10.37107/jhas.118 ◽

2019 ◽

Vol 9 (2) ◽

pp. 1-5

Author(s):

Atisammodavardhana Kaundinnyayana ◽

Anita G.C ◽

Asmita Banstola ◽

Kishwor Poudel ◽

Nirmal Acharya ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Positive Control ◽

Lagenaria Siceraria ◽

Benincasa Hispida ◽

Ic50 Values ◽

In Vitro Antioxidant Activity

Introduction: Diabetes mellitus is an endocrine and metabolic disorder characterized by the defect in insulin secretion or insulin action or both. The association between diabetes mellitus and oxidative stress is well established. Cucurbitaceae is the largest family containing 120 genera mostly grown for its sweet and juicy fruit in warm climates all over the world. In the present work ethanolic extracts of the seeds of Trichosanthes cucumerina Linn (Chichinno), Lagenaria siceraria (Lauka), Cucurbita pepo (Pharsi), Luffa aegiptiaca (Ghiraula), and Benincasa hispida (Kubindo) collected from Western Nepal has been evaluated for their in vitro antioxidant activity and in vivo hypoglycemic effects. Methods: In vitro antioxidant activity was assessed by using DPPH free radical scavenging activities and their IC50 values were calculated. In vivo hypoglycemic effect was examined on normoglycemic rats. The clinical significance of ethanolic extract at the doses of 250 and 500 mg/kg body weight was investigated in 0, 30, 60, 120 and 180 minutes of oral administration. Metformin treated group was used as the positive control. Results: For DPPH radical scavenging action, the IC50 values of Trichosanthes cucumerina, Luffa aegiptiaca, Benincasa hispida, Cucurbita pepo, Lagenaria siceraria were found to be 60.72, 127.73, 49.63, 98.16 and 52.46 µg/ml respectively which were compared to the IC50 value of ascorbic acid (Positive control) which was found to be 38.11 µg/ml. The extracts of Benincasa hispida, and were having higher antioxidant activity and tested for in vivo hypoglycemic activity.In vivo administration of two doses of ethanolic extract of Lagenaria siceraria reduced the level of blood glucose while the best result was obtained at 250 mg/kg. Conclusions: Present study revealed promising antioxidant and hypoglycemic activity of ethanolic extract of Lagenaria siceraria. The further exploration of Lagenaria siceraria for its effective use in the traditional medicinal system is essential.

Download Full-text

Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

Revista Facultad de Ciencias Básicas ◽

10.18359/rfcb.2755 ◽

2017 ◽

Vol 13 (1) ◽

pp. 56-59

Author(s):

Aura M. Blandón ◽

Oscar M. Mosquera ◽

Antônio E. G. Sant’ana ◽

Aldenir F. Dos Santos ◽

Luana L. S. Pires

Keyword(s):

Antioxidant Activity ◽

Plant Species ◽

Plant Extracts ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Dpph Assay ◽

Methanolic Extracts ◽

In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

Download Full-text

In Vitro Antioxidant Activity of Camellia japonica L

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.518-523.5555 ◽

2012 ◽

Vol 518-523 ◽

pp. 5555-5558

Author(s):

Bao Qing Wang

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Methanol Extract ◽

Radical Scavenging ◽

Camellia Japonica ◽

Methanol Extracts ◽

Ic50 Values ◽

Acid Test ◽

Acetone Extracts ◽

Β Carotene

Antioxidant activities of acetone and methanol extracts from stems and leaves of Camellia japonica L, one of traditional Chinese medicine consumed in the eastnorth region of China, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In the DPPH system, the antioxidant activity of the acetone extracts was superior to that of the methanol extracts, with IC50 values were 246.56 and 320.17μg/mL, respectively. In the β-carotene-linoleic acid test, the inhibition ratio of the acetone extracts was found to be the higher, compared with the methanol extracts, with IC50 values were 258.19 and 396.88 μg/mL In general, the methanol extract showed greater activity than the acetone extract in both systems, such results might be attributed to the effects of the components of the methanol extract. It was concluded that Camellia japonica L might be a potential natural source of antioxidants.

Download Full-text

Exploitation of the Antioxidant Potential of Geranium Macrorrhizum (Geraniaceae): Hepatoprotective and Antimicrobial Activities

Natural Product Communications ◽

10.1177/1934578x1200701218 ◽

2012 ◽

Vol 7 (12) ◽

pp. 1934578X1200701 ◽

Cited By ~ 4

Author(s):

Niko S. Radulović ◽

Milan B. Stojković ◽

Snežana S. Mitić ◽

Pavle J. Randjelović ◽

Ivan R. Ilić ◽

...

Keyword(s):

Antimicrobial Activity ◽

Plant Species ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Antimicrobial Activities ◽

Antioxidant Potential ◽

Human Pathogens ◽

Methanol Extracts

In this study we evaluated in vitro (radical scavenging) and in vivo (hepatoprotective effect) antioxidant activities and antimicrobial properties of the extracts of the above- and underground parts of Geranium macrorrhizum L. (Geraniaceae), an ethnopharmacologically renowned plant species. The antioxidant activity and total phenol and flavonoid contents of four different solvent extracts were evaluated by seven different methods. The methanol extracts, administered i.p. to rats (120-480 mg/kg), were evaluated for hepatoprotective activity in a CCl4-induced hepatotoxicity model. The same extracts were tested for antimicrobial activity against seven bacterial and two fungal species. The administered methanol extracts with the highest antioxidant potential showed a significant dose-dependent hepatoprotective action against CCl4-induced liver damage in both decreasing the levels of liver transaminases and bilirubin and in reducing the extent of morphological malformations of the liver. The leaf methanol extract displayed a very strong antibacterial activity, especially against Staphylococcus aureus, with low minimal inhibitory and bactericidal concentrations. These results justify the frequent use of this plant species in folk medicine. Besides the known astringent effect, one can expect that the observed antimicrobial activity against several human pathogens contributes to the wound healing properties of this plant.

Download Full-text

Evaluation of Some Biological Applications of Pleurotus citrinopileatus and Boletus edulis Fruiting Bodies

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190904162403 ◽

2019 ◽

Vol 20 (15) ◽

pp. 1309-1320 ◽

Cited By ~ 1

Author(s):

Ahmed M. Younis ◽

Marwa M. Abdel-Aziz ◽

Mohamed Yosri

Keyword(s):

Antioxidant Activity ◽

Flow Cytometric Analysis ◽

Radical Scavenging ◽

Biologically Active ◽

Fruiting Bodies ◽

Methanol Extracts ◽

Boletus Edulis ◽

Glucosidase Activity ◽

Hela Cell Lines ◽

Pleurotus Citrinopileatus

Background:: Mushrooms are deemed as a special delicacy in many countries. They are considered an important cuisine due to their bioactive ingredients and possible health benefits. Methods: Herein, we measured selected biological properties of methanol extracts of Pleurotus citrinopileatus and Boletus edulis fruiting bodies including; in vitro antimicrobial activity, anti-α- glucosidase activity, antioxidant activity, anti-lipase activity and cytotoxic activity against different cancer cells and normal cells. Results: B. edulis methanol extracts showed high antimicrobial and anti-α-glucosidase activity. In contrast, P. citrinopileatus methanol extracts showed superior antioxidant activity indicated by (1,1- diphenyl-2-picrylhydrazyl) DPPH radical scavenging with half maximal inhibitory concentration of IC50 37.4 µg/ml, anti-lipase activities with IC50 65.2 µg/ml and high cytotoxicity activity against HepG2 and HeLa cell lines with IC50 22.8 and 36.7 µg/ml, respectively. Flow cytometric analysis of the cell cycle was used to show apoptotic effects of methanol extracts against HepG2 and HeLa cells. Conclusion: P. citrinopileatus and B. edulis methanolic extracts appear to contain biologically active compounds that might be used to treat some common human diseases.

Download Full-text

Current Status and Future Perspective for Research on Medicinal Plants with Anticancerous Activity and Minimum Cytotoxic Value

Current Drug Targets ◽

10.2174/1389450120666190429120314 ◽

2019 ◽

Vol 20 (12) ◽

pp. 1227-1243

Author(s):

Hina Qamar ◽

Sumbul Rehman ◽

D.K. Chauhan

Keyword(s):

Side Effects ◽

Medicinal Plants ◽

Anticancer Agents ◽

Drug Targets ◽

Psidium Guajava ◽

Current Status ◽

Future Perspective ◽

Wide Range

Cancer is the second leading cause of morbidity and mortality worldwide. Although chemotherapy and radiotherapy enhance the survival rate of cancerous patients but they have several acute toxic effects. Therefore, there is a need to search for new anticancer agents having better efficacy and lesser side effects. In this regard, herbal treatment is found to be a safe method for treating and preventing cancer. Here, an attempt has been made to screen some less explored medicinal plants like Ammania baccifera, Asclepias curassavica, Azadarichta indica, Butea monosperma, Croton tiglium, Hedera nepalensis, Jatropha curcas, Momordica charantia, Moringa oleifera, Psidium guajava, etc. having potent anticancer activity with minimum cytotoxic value (IC50 >3μM) and lesser or negligible toxicity. They are rich in active phytochemicals with a wide range of drug targets. In this study, these medicinal plants were evaluated for dose-dependent cytotoxicological studies via in vitro MTT assay and in vivo tumor models along with some more plants which are reported to have IC50 value in the range of 0.019-0.528 mg/ml. The findings indicate that these plants inhibit tumor growth by their antiproliferative, pro-apoptotic, anti-metastatic and anti-angiogenic molecular targets. They are widely used because of their easy availability, affordable price and having no or sometimes minimal side effects. This review provides a baseline for the discovery of anticancer drugs from medicinal plants having minimum cytotoxic value with minimal side effects and establishment of their analogues for the welfare of mankind.

Download Full-text

Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽

10.3390/molecules26092555 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2555

Author(s):

Sang Koo Park ◽

Yoon Kyung Lee

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Scavenging Activity ◽

Polyphenol Content ◽

Abts Assay ◽

Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

Download Full-text

Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽

10.3390/antiox10010032 ◽

2020 ◽

Vol 10 (1) ◽

pp. 32

Author(s):

Pattamaporn Aksornchu ◽

Netima Chamnansilpa ◽

Sirichai Adisakwattana ◽

Thavaree Thilavech ◽

Charoonsri Choosak ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Digestive Enzyme ◽

Radical Scavenging ◽

Protein Glycation ◽

Trolox Equivalent Antioxidant Capacity ◽

Fruit Extract ◽

Antioxidant Power ◽

Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

Download Full-text

A Review of Plants Used in South African Traditional Medicine for the Management and Treatment of Hypertension

Planta Medica ◽

10.1055/a-0801-8771 ◽

2018 ◽

Vol 85 (04) ◽

pp. 312-334 ◽

Cited By ~ 10

Author(s):

Fatai Balogun ◽

Anofi Ashafa

Keyword(s):

South Africa ◽

Medicinal Plants ◽

South African ◽

Plant Species ◽

Agricultural Research ◽

Higher Plants ◽

In Vivo Studies ◽

Research Councils

AbstractSouth Africa contains 9% of the worldʼs higher plants, and despite its rich biodiversity, it has one of the highest prevalence of hypertension in Africa. This review provides information on medicinal plants embraced in South Africa for hypertension management, with the aim of reporting pharmacological information on the indigenous use of these plants as antihypertensives. This review not only focuses on the activity of antihypertensive medicinal plants but also reports some of its phytochemical constituents and other ethnopharmacological and therapeutic properties. Information obtained from scientific and or unpublished databases such as Science Direct, PubMed, SciFinder, JSTOR, Google Scholar, Web of Science, and various books revealed 117 documented antihypertensive plant species from 50 families. Interestingly, Asteraceae topped the list with 16 species, followed by Fabaceae with 8 species; however, only 25% of all plant species have demonstrated antihypertensive effects originating from both in vitro and in vivo studies, lending credence to their folkloric use. Only 11 plant species reportedly possess antihypertensive properties in animal models, with very few species subjected to analytical processes to reveal the identity of their bioactive antihypertensive compounds. In this review, we hope to encourage researchers and global research institutions (universities, agricultural research councils, and medical research councils), particularly those showing an interest in natural products, for the need for concerted efforts to undertake more studies aimed at revealing the untapped potential of these plants. These studies are very important for the development of new pharmaceuticals of natural origin useful for the management of hypertension.

Download Full-text

RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14964 ◽

2016 ◽

Vol 8 (12) ◽

pp. 236 ◽

Cited By ~ 4

Author(s):

Ade Chandra Iwansyah ◽

Rizal M. Damanik ◽

Lilik Kustiyah ◽

Muhammad Hanafi

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Total Phenolics ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Nutritional Composition ◽

Total Flavonoids ◽

Wide Range ◽

Phenolics Content

Objective: The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.Methods: A wide range of analytical parameters was studied, including yield; pH; water activity (aw); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.Results: All of the galactagogue herbs, viz. Plectranthus amboinicus (Lour.) (PA), Sauropus androgynus (SA), Moringa oleifera (MO), and Carica papaya leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC50), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO>CA>PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.Conclusion: This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.

Download Full-text