A Cross Sectional Study to Determine Association of the Socio Demographic Risk Factors with Prevalence and Virulence of H. pylori Infection in Salivary Samples of Asymptomatic Subjects by Application of Polymerase Chain Reaction Technique

Pharmacologia ◽  
2012 ◽  
Vol 3 (10) ◽  
pp. 481-489 ◽  
Author(s):  
Subhash Laxmanrao Bodhankar ◽  
Pinaki Ghosh
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Polymerase Chain ◽  
H Pylori ◽  
Demographic Risk ◽  
Demographic Risk Factors ◽  
Asymptomatic Subjects

West Nile Virus Chorioretinitis in the Presence of Negative Cerebrospinal Fluid Polymerase Chain Reaction Results

2021 ◽  
pp. 247412642097925
Author(s):  
Kareem Moussa ◽  
Karen W. Jeng-Miller ◽  
Leo A. Kim ◽  
Dean Eliott
Keyword(s):  
Cerebrospinal Fluid ◽  
West Nile Virus ◽  
Cross Sectional Study ◽  
Nucleic Acid Amplification ◽  
Sectional Study ◽  
Chain Reaction ◽  
West Nile ◽  
Cross Sectional ◽  
Positive Serum ◽  
Polymerase Chain

Purpose: This work aims to evaluate the utility of nucleic acid amplification testing (NAAT) and serology in confirming West Nile Virus (WNV) infection in patients with suspected WNV chorioretinitis. Methods: A retrospective cross-sectional study was conducted of a cluster of patients who presented to the Retina Service of Massachusetts Eye and Ear between September and October 2018. Results: Three patients were identified with classic WNV chorioretinitis lesions with negative cerebrospinal fluid NAAT and positive serum serology findings. The diagnosis of WNV chorioretinitis was made based on the appearance of the fundus lesions and the presence of characteristic findings on fluorescein angiography as previously described in the literature. Conclusions: This report highlights 3 unique cases of WNV chorioretinitis in which NAAT of cerebrospinal fluid failed to identify WNV as the inciting agent. These cases stress the importance of serum serologic testing in diagnosing WNV infection.

scholarly journals Prevalence of Cryptosporidiosis in Crossbred Calves in Two Selected Areas of Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 185-190
Author(s):  
A Khair ◽  
MM Alam ◽  
AKMA Rahman ◽  
M Shahiduzzaman ◽  
MS Parvez ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Clinical Status ◽  
Cross Sectional Study ◽  
Positive Control ◽  
Age Group ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Fecal Samples ◽  
Polymerase Chain

A cross-sectional study was conducted to determine the prevalence of bovine cryptosporidiosis using 110 fecal samples of crossbred diarrhoeic calves from two different areas (Muktagacha, Mymensingh and Shajadpur, Sirajgonj) in Bangladesh during April 2012 to September 2014. The fecal samples were screened by rapid detection kit and confirmed by Modified Ziehl- Neelsen staining, and polymerase chain reaction (PCR). The positive samples along with standard positive control yielded 1325bp band on PCR. The overall prevalence of cryptosporidiosis in crossbred calves was 28.18% (31/110) by rapid detection kit. The higher prevalence of cryptosporidiosis was found in the calves from Shajadpur (29.76%) than the calves from Muktagacha (23.08%).The prevalence of cryptosporidiosis was significantly (p<0.001) higher in calves between 1-2 months (70%) age group than less than one month age group (24.49%). Cryptosporidiosis was not observed in calves over two months age. The prevalence of cryptosporidiosis was higher in males (34.75%) than females (24.64%) although not significant statistically. It is evident that the prevalence of cryptosporidiosis in bovine in these areas is under diagnosed and the clinical status of infection is potentially high.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21288 Bangl. J. Vet. Med. (2014). 12 (2): 185-190 

A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece

2002 ◽  
Vol 109 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Leonidas S Leontides ◽  
Manolis N Saridomichelakis ◽  
Charalambos Billinis ◽  
Vasilios Kontos ◽  
Alexander F Koutinas ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Leishmania Spp ◽  
Healthy Dogs ◽  
Polymerase Chain

Detection of blaVIM and blaIMP Carbapenemase-Producing Pseudomonas aeruginosa from Clinical Samples in Iran

2021 ◽  
Author(s):  
Mohammad Reza Zolfaghari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cross Sectional Study ◽  
Clinical Samples ◽  
Sectional Study ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Cross Sectional ◽  
High Prevalence ◽  
Polymerase Chain ◽  
High Level

Background and Aims: The prevalence of carbapenemase-producing Pseudomonas aeruginosa (P. aeruginosa) strains has been recently reported worldwide. Therefore, accurate and rapid detection of carbapenemase-producing isolates is essential. So, this study aimed to detect blaVIM and blaIMP carbapenemase-producing strains using the modified Hodge test (MHT) and reverse transcription-polymerase chain reaction (RT-PCR). Materials and Methods: In this cross-sectional study, P. aeruginosa  strains were collected from clinical samples (blood, urine, wound, and other liquids body) in Firoozgar and Shahid Motahari Hospitals in Tehran and Velayat Hospital in Rasht Province, from May to December 2018. After identifying the isolates using the standard microbial tests, carbapenemase-producing strains were isolated by the modified hodge test. After that, the detection of blaVIM and blaIMP genes was performed by RT-PCR technique. Results: One hundred P. aeruginosa were isolated from different clinical samples. Among these, 74 (74%) isolates were considered as carbapenemase positive using MHT. The frequencies of blaVIM and blaIMP genes were obtained as 83% and 11%, respectively. Conclusions: The results of this study indicate a high level of resistance to most of the antibiotics tested and a high prevalence of blaVIM gene in P. aeruginosa strains.

scholarly journals Headache associated with COVID-19: Frequency, characteristics and association with anosmia and ageusia

Cephalalgia ◽  
2020 ◽  
Vol 40 (13) ◽  
pp. 1443-1451 ◽  
Author(s):  
Pedro Augusto Sampaio Rocha-Filho ◽  
João Eudes Magalhães
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chi Square ◽  
Cross Sectional ◽  
Severe Intensity ◽  
Chi Square Test ◽  
Polymerase Chain

Objectives To assess the frequency and characteristics of headache in patients with COVID-19 and whether there is an association between headache and anosmia and ageusia. Methods This was a cross-sectional study. Consecutive patients admitted to hospital with COVID-19, confirmed by reverse transcription polymerase chain reaction (RT-PCR) technique, were assessed by neurologists. Results Seventy-three patients were included in the study, 63% were male; the median age was 58 years (IQR: 47–66). Forty-seven patients (64.4%) reported headaches, which had most frequently begun on the first day of symptoms, were bilateral (94%), presenting severe intensity (53%) and a migraine phenotype (51%). Twelve patients (16.4%) presented with headache triggered by coughing. Eleven (15%) patients reported a continuous headache. Twenty-eight patients (38.4%) presented with anosmia and 29 (39.7%) with ageusia. Patients who reported hyposmia/anosmia and/or hypogeusia/ageusia experienced headache more frequently than those without these symptoms (OR: 5.39; 95% CI:1.66–17.45; logistic regression). Patients with anosmia and ageusia presented headache associated with phonophobia more often compared to those with headache without these complaints (Chi-square test; p < 0.05). Headache associated with COVID-19 presented a migraine phenotype more frequently in those experiencing previous migraine ( p < 0.05). Conclusion Headaches associated with COVID-19 are frequent, are generally severe, diffuse, present a migraine phenotype and are associated with anosmia and ageusia.

scholarly journals Association between VEGF -2578 C> A Polymorphism with VEGF Serum Levels in Gastritis with Helicobacter Pylori

2021 ◽  
Vol 8 (8) ◽  
pp. 171-175
Author(s):  
Tara Rizvira Monica ◽  
Gontar Alamsyah Siregar ◽  
Taufik Sungkar
Keyword(s):  
Serum Levels ◽  
Mucosal Damage ◽  
Cross Sectional Study ◽  
Endoscopic Examination ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Excess Production ◽  
Polymerase Chain ◽  
H Pylori ◽  
The Relationship

Mucosal damage in people with gastritis causes the production of VEGF. VEGF is a neoangiogenesis function to repair damaged tissue. Excess production can cause cancer risk. VEGF genotype polymorphisms are thought to affect the production of serum VEGF levels. The aim of this study was to determine the relationship between VEGF - 2578 C> A polymorphism and serum VEGF levels in H. pylori gastritis. Method: cross-sectional study was conducted at H. Adam Malik General Hospital and Network Hospital with 100 samples. Endoscopic examination was performed to assess the gastric mucosa and a tissue biopsy was performed. The urea breath test (UBT) test and the Campylobacter like organism (CLO) test to determine H. pylori infection. VEGF - 2578 C> A was checked by Polymerase Chain Reaction (PCR). The data will be analyzed by univariate and bivariate. Result: One hundred people with gastritis, of which 59 people were infected with H. pylori. In this study, H. pylori infection did not have a significant relationship with VEGF levels. VEGF - 2578 C> A polymorphisms also had no relationship to serum VEGF levels. Conclusion: There is no correlation between VEGF - 2578 C> A polymorphism with VEGF serum levels (p> 0.05). Keywords: VEGF polymorphisms, VEGF - 2578 CA, H. pylori, Gastritis.

scholarly journals Severity assessment of single dose Oxford-AstraZeneca vaccinated individuals infected with SARS CoV-2 in the Southeast Bangladesh

2021 ◽  
Author(s):  
Eaftekhar Ahmed Rana ◽  
Pronesh Dutta ◽  
Md. Sirazul Islam ◽  
Tanvir Ahmad Nizami ◽  
Tridip Das ◽  
...  
Keyword(s):  
Health Risk ◽  
Good Health ◽  
Cross Sectional Study ◽  
Significant Finding ◽  
Sectional Study ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Infection Period ◽  
Polymerase Chain ◽  
Taste And Smell

The present global endeavor to uncover the most effective vaccines against severe acute respiratory syndrome coronavirus (SARS-CoV-2) that can tremendously prevent transmission, infection and significantly reduce public health risk. COVID-19 vaccination program is underway in different parts of the world including Bangladesh but till to date there is no available health data revealed among the vaccinated peoples. We conducted a cross-sectional study from February 15 to April 15, 2021 to assess the health status of 1st dose Oxford-AstraZeneca vaccinated individuals infected with SARS CoV-2. Standard virological method, real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was performed to detect SARS-CoV-2 and the different health parameters from vaccinated individuals were collected through direct mobile phone contact using pre-structured questionnaires. A total of 6146 suspected samples were tested and 1752 were found positive for SARS-CoV-2, of them 200 individuals were identified who received 1st dose of COVID-19 vaccine. Within the test period, majority of male (65.6%) and female (34.4%) carried moderate numbers of viruses which comprise between 30.01-35 cyclic threshold (ct) values. Among the vaccinated individuals, 165 (82.5%; 95% CI: 76.51 - 87.5) persons were not hospitalized and 177 (88.5%; 95% CI: 83.24 - 92.57) did not show any respiratory difficulties. Only a few (16) (8%; 95% CI: 4.64 - 12.67) of COVID-19 positive patients needed extra oxygen support and 199 (99.5%; 95% CI: 97.25 - 99.99) individuals did not require any intensive care unit (ICU) interference. Overall, oxygen saturation was recorded around 96.8% and respiratory difficulties did not extend more than 5 days, irrespective of age and sex during the infection period. Within the vaccinated COVID-19 positive individuals 113 (56.5%; 95% CI: 49.33 - 63.48) and 111(55.5%; 95% CI: 48.32 - 62.51) persons have normal physiological taste and smell. However, we have found a larger proportion of vaccinated persons (129) (64.5%; 95% CI: 57.44 - 71.12) carrying different comorbidity, among them high blood pressure 36 (27.9%; 95% CI, 20.37 - 36.48) and diabetes 32 (24.8%; 95% CI: 17.63 - 33.18) were found more prevalent. Moreover, the significant finding of the present study was 199 (99.5%; 95% CI: 97.25 - 99.99) vaccinated individuals survived with good health conditions and became negative in RT-qPCR. The authors suggest that health risk assessment among the COVID-19 vaccinated persons when infected with SARS-CoV-2 is crucial and time demanding task for the whole world. However, the present study illustrates that the administration of the 1st dose Oxford-AstraZeneca vaccine significantly reduces health risk during the COVID-19 infection period.

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