scholarly journals Hemocytes and humoral factors in silkworm blood are cooperatively involved in sheep erythrocyte aggregation

2011 ◽  
Vol 5 (3) ◽  
pp. 125-129 ◽  
Author(s):  
Katsutoshi Imamura ◽  
Kenichi Ishii ◽  
Hiroshi Hamamoto ◽  
Kazuhisa Sekimizu
Keyword(s):  
Sheep Erythrocyte ◽  
Erythrocyte Aggregation ◽  
Humoral Factors

The response of skin perfusion and of rheological and immunological variables to intravenous prostanoid administration in Raynaud’s phenomenon secondary to collagenosis

VASA ◽  
2005 ◽  
Vol 34 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Drinda ◽  
Neumann ◽  
Pöhlmann ◽  
Vogelsang ◽  
Stein ◽  
...  
Keyword(s):  
Laser Doppler Flowmetry ◽  
Raynaud’S Phenomenon ◽  
Raynaud's Phenomenon ◽  
Plasma Viscosity ◽  
Laser Doppler ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Long Term Effects ◽  
Doppler Flowmetry

Background: Prostanoids are used in the treatment of Raynaud’s phenomenon and acral perfusion disorders secondary to collagenosis. In subjective terms, intravenous administration of these agents produces success in more than 50% of patients. The therapeutic outcome of clinical administration of alprostadil or iloprost may vary from individual to individual. Patients and methods: The following variables were analysed in a cross-over study in 27 patients with collagenosis and Raynaud’s phenomenon: plasma viscosity and erythrocyte aggregation (rheological variables), partial pressure of oxygen and laser Doppler flowmetry in the finger region, and lymphocyte phenotyping and interleukin (IL) determinations (immunological variables). Results: Laser Doppler flowmetry revealed significant differences between patients with secondary Raynaud’s phenomenon and a control group of 25 healthy subjects. Laser Doppler readings did not change significantly as a result of the treatments. Therapy with iloprost produced a reduction in IL-1beta, L-selectin (CD 62 L) and IL-6. Conclusion: The change in immunological variables due to iloprost may explain the long-term effects of prostaglandins in the treatment of Raynaud’s phenomenon. From our results it is not possible to infer any preference for iloprost or alprostadil.

Bovine hypodermatosis in Stavropol region: distribution, pathogenesis, veterinary and sanitary assessment of slaughter products

2020 ◽  
pp. 7-10
Author(s):  
Vasiliy P. Petropavlovsky ◽  
◽  
Vyacheslav V. Marchenko ◽  
Lyudmila S. Vikulova ◽  
Olga D. Chepeleva ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Climatic Conditions ◽  
The Body ◽  
Erythrocyte Aggregation ◽  
Economic Damage ◽  
Hydropic Degeneration ◽  
Hypoderma Lineatum ◽  
Hypoderma Bovis ◽  
Stavropol Region ◽  
Histomorphological Changes

Summary. Data on distribution of hypodermatosis among large horned cattle in Stavropol region are presented in the article. It was proved that this pathology is widespread in this region. From 12% to 38% of animals from the total number of examined livestock are affected. Ordinary subcutaneous botfly (stroma) Hypoderma bovis de Geer and south subcutaneous botfly (pischevodnik) Hypoderma lineatum de Villers parasites on large horned cattle. The species ratio of gadflies is: Hypoderma bovis (de Gee - 61.1%, Hypoderma lineatum de Villers - 38.9%). The disease is widespread in all climatic zones of Stavropol region. Animals of all age and sex groups are infected, but young animals are more often affected. Linear relationship between the indicators of the transformation of climatic conditions in Stavropol region, the spread of hypodermatosis and the timing of treatment and preventive measures were determined. Authors proved that the complete death of H. bovis and H. lineatum imago (regardless of the time of their hatching) was noted when the temperature drops to -3°C for two days. Thus, this temperature determines the start of treatment and preventive measures against hypodermatosis in the area of animal welfare. When analyzing the histomorphological changes caused by H. bovis and H. lineatum in the body of large horned cattle, perforation of the skin, swelling of adjacent tissues, disorganization of the stroma and structural elements, erythrocyte aggregation, hemorrhages, hydropic degeneration of cellular elements, tissue necrosis, polymorphonuclear leukocyte infiltration of affected organs and tissues were observed. The data on pathogenesis were presented, the analysis of economic damage and the veterinary and sanitary assessment of the products of slaughter of cattle with hypodermatosis were carried out.

Alcohol Intake and Erythrocyte Aggregation

1955 ◽  
Vol 16 (4) ◽  
pp. 614-618 ◽  
Author(s):  
O. Forsander ◽  
Heikki Suomalainen
Keyword(s):  
Alcohol Intake ◽  
Erythrocyte Aggregation

Humoral factors of innate immunity in the saliva of patients with infectious inflammatory respiratory diseases

2018 ◽  
pp. 13-17
Author(s):  
Oleg Melnikov ◽  
Diana Zabolotnaya ◽  
Alexander Bredun ◽  
Bogdan Bil ◽  
Oksana Rylska ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Chronic Rhinosinusitis ◽  
Inflammatory Diseases ◽  
Activity Level ◽  
Chronic Tonsillitis ◽  
Control Group ◽  
Quantitative Composition ◽  
Upper Airways ◽  
Humoral Factors ◽  
Immune Modulators

Introduction: In recently ears factors of innate immunity both cellular and humoral have been paid considerable attention as they are a protective barrier of a fast response and that is why they are largely concentrated at the intersection of the digestive tract and airways. The data concerning the activity level of factors of innate immunity in the upper airways affected by nonspecific inflammatory processes is insufficient and sparse and therefore the purpose of this research was to study the content of humoral factors of innate immunity in the oropharyngeal secretion (ORS) of patients with chronic infectious inflammatory diseases of the upper airways in remission. Materials and Methods: There was an examination of 16 patients with chronic rhinosinusitis (CRS) of bacterial genesis (15-40 years old), 12 patients with rhinopharyngitis of post-viral genesis (8-16 years old), 12 people with scleroma from 30 tо 52 years of age (atrophic form), 10 patients with chronic tonsillitis in remission (from 10 to 33 years of age) and 11 patients of a control group (practically healthy donors from 12 tо 40 years of age). The content of MIP-1b, defensin-1β, lactoferrin, lysozyme, α-interferon was studied in the nonstimulated OPS. Statistics were carried out using Mann-Whitney U-test. Results: The greatest number of deviations in the decrease in the content of the examined nonspecific protective factors was found in cases of scleroma, chronic tonsillitis and chronic rhinosinusitis (p<0,05). The lack of protective humoral factors of innate immunity can be evidence of local immunodeficiency even in remission, which is a pathophysiological component of the maintenance of chronic inflammation. Conclusion: The decrease int he quantitative composition of factors of innate immunity in the oropharyngeal secretion of patients with chronic infectious inflammatory diseases of the airways is an objective ground not only for a replacement therapy, but also for the use of immune response modifiers from photo-immune modulators to “genuine immune modulators” controlling the state of the factors of both innate immunity and immunoglobulins, primarily of secretory type, the level and functionality of various groups of immunocompetent and accessory cells.

Methodological Aspects of Erythrocyte Aggregation

2007 ◽  
Vol 2 (3) ◽  
pp. 240-245 ◽  
Author(s):  
Alexander Pribush ◽  
Naomi Meyerstein
Keyword(s):  
Erythrocyte Aggregation ◽  
Methodological Aspects

The role of microRNA in hepatic ischemia/reperfusion injury

MicroRNA ◽  
2020 ◽  
Vol 09 ◽  
Author(s):  
Chrysanthos D. Christou ◽  
Georgios Tsoulfas
Keyword(s):  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Clinical Manifestations ◽  
Diagnostic Procedures ◽  
Clinical Tool ◽  
Hepatic Ischemia ◽  
Humoral Factors ◽  
Early Graft ◽  
Hepatic Ischemia Reperfusion

Introduction: Ischemia-reperfusion (I/R) injuries are caused by complex interrelated mechanisms and pathways. Regarding the liver, I/R injuries and their clinical manifestations are crucial for the surgical outcome. Despite its importance, there is no broadly accepted therapy either for the prevention or for the management of I/R injury. I/R injury of the liver can occur either during hepatic surgery (warm) or during the transplantation procedure (cold). MicroRNAs play a pivotal role in the mechanism of I/R injury, as they regulate the expression of the cellular participants and humoral factors associated with I/R injury. Objective: In this review, we highlight the microRNAs that are involved in the I/R injury of the liver, and the molecular pathways that they regulate. In addition, we discuss the potential role of circulating microRNAs as biomarkers and their role as pharmacological targets in the prevention, diagnosis and treatment of I/R injuries. Method: We conducted a comprehensive review of the PubMed bibliographic database regarding microRNAs and I/R injuries of the liver. Results: In diagnostics, microRNA panels could replace invasive diagnostic procedures, relieving patients of the associated complications. In therapeutics, microRNA agomirs, antagomirs and other drugs can be used to shift the balance between proapoptotic and survival pathways, to alleviate the liver damage caused by I/R. In transplantation procedures, microRNA profiling could decrease the incidence of early graft dysfunction, especially regarding marginal grafts. Conclusion: Although microRNAs seem a very promising clinical tool in the management of I/R injuries, further research is required, until microRNAs become a novel tool in the diagnosis and monitoring of an I/R injury of the liver.

scholarly journals Development of Injectable Polydactyly-Derived Chondrocyte Sheets

2021 ◽  
Vol 22 (6) ◽  
pp. 3198
Author(s):  
Shiho Wasai ◽  
Eriko Toyoda ◽  
Takumi Takahashi ◽  
Miki Maehara ◽  
Eri Okada ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cartilage Damage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Invasive Treatment ◽  
Experimental Conditions ◽  
Humoral Factors ◽  
Less Invasive ◽  
Cell Counts ◽  
Invasive Method ◽  
Injection Control

We are conducting a clinical study of the use of allogeneic polydactyly-derived chondrocyte sheets (PD sheets) for the repair of articular cartilage damage caused by osteoarthritis. However, the transplantation of PD sheets requires highly invasive surgery. To establish a less invasive treatment, we are currently developing injectable fragments of PD sheets (PD sheets-mini). Polydactyly-derived chondrocytes were seeded in RepCell™ or conventional temperature-responsive inserts and cultured. Cell counts and viability, histology, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), and flow cytometry were used to characterize PD sheets-mini and PD sheets collected from each culture. To examine the effects of injection on cell viability, PD sheets-mini were tested in four experimental conditions: non-injection control, 18 gauge (G) needle, 23G needle, and syringe only. PD sheets-mini produced similar amounts of humoral factors as PD sheets. No histological differences were observed between PD sheets and PD sheets-mini. Except for COL2A1, expression of cartilage-related genes did not differ between the two types of PD sheet. No significant differences were observed between injection conditions. PD sheets-mini have characteristics that resemble PD sheets. The cell viability of PD sheets-mini was not significantly affected by needle gauge size. Intra-articular injection may be a feasible, less invasive method to transplant PD sheets-mini.

scholarly journals Effect of Platelet-Rich Plasma on M1/M2 Macrophage Polarization

2021 ◽  
Vol 22 (5) ◽  
pp. 2336
Author(s):  
Ryoka Uchiyama ◽  
Eriko Toyoda ◽  
Miki Maehara ◽  
Shiho Wasai ◽  
Haruka Omura ◽  
...  
Keyword(s):  
Culture Media ◽  
Platelet Rich Plasma ◽  
Point Of Care ◽  
Macrophage Polarization ◽  
Osteoarthritis Of The Knee ◽  
M2 Macrophage ◽  
Related Factors ◽  
Humoral Factors ◽  
M1 Macrophage ◽  
M2 Macrophage Polarization

Osteoarthritis of the knee (OAK) is a chronic degenerative disease and progresses with an imbalance of cytokines and macrophages in the joint. Studies regarding the use of platelet-rich plasma (PRP) as a point-of-care treatment for OAK have reported on its effect on tissue repair and suppression of inflammation but few have reported on its effect on macrophages and macrophage polarization. Based on our clinical experience with two types of PRP kits Cellaid Serum Collection Set P type kit (leukocyte-poor-PRP) and an Autologous Protein Solution kit (APS leukocyte-rich-PRP), we investigated the concentrations of humoral factors in PRPs prepared from the two kits and the effect of humoral factors on macrophage phenotypes. We found that the concentrations of cell components and humoral factors differed between PRPs purified using the two kits; APS had a higher concentration of M1 and M2 macrophage related factors. The addition of PRP supernatants to the culture media of monocyte-derived macrophages and M1 polarized macrophages revealed that PRPs suppressed M1 macrophage polarization and promoted M2 macrophage polarization. This research is the first to report the effect of PRPs purified using commercial kits on macrophage polarization.

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