scholarly journals Methyl jasmonate vapors affect the composition and peroxidation of major fatty acids in common buckwheat seedlings

2016 ◽  
Vol 69 (4) ◽  
Author(s):  
Józef Klocek ◽  
Magdalena Szwed ◽  
Danuta Koczkodaj ◽  
Joanna Mitrus ◽  
Marian Saniewski ◽  
...  

The effect of methyl jasmonate (MJ) vapors on the composition and peroxidation of major fatty acids in the organs of common buckwheat seedlings was investigated. The composition of fatty acids in the hypocotyl and cotyledons of seedlings changed significantly under exposure to MJ vapors in closed jars. Four-day exposure to MJ led to a significant reduction in the concentrations of stearic, linoleic, and linolenic acids in the hypocotyl, whereas oleic acid levels increased approximately 3.5-fold. A decrease in stearic acid levels and an increase in the content of linolenic acid were noted in cotyledons, whereas oleic acid levels decreased in roots. Seven-day exposure to MJ vapor caused a further reduction in stearic acid content and an increase in oleic acid and linoleic acid levels in the hypocotyl. At the same time, the linoleic acid content of roots and linolenic acid levels in cotyledons were doubled, but a 5-fold reduction in linolenic acid concentrations was observed in roots. Methyl jasmonate intensified fatty acid peroxidation in cotyledons after 4 and 7 days and in roots after 4 days of exposure. Peroxidation was inhibited in the hypocotyl and roots after 7 days. The noted changes in the composition and peroxidation of fatty acids are probably indicative of senescence in buckwheat seedlings under the influence of MJ. Senescence seems to proceed faster in cotyledons than in other organs of buckwheat seedlings.

Author(s):  
Vilcacundo E ◽  
Alvarez M ◽  
Silva M ◽  
Carpio C ◽  
Morales D ◽  
...  

 Objective: The aim of this study was to determine the fatty acids composition in a tocte seeds oil (Juglans neotropica Diels) sample cultivated in Ecuador.Methods: Tocte oil was obtained from tocte seeds using the cold pressing method. Fatty acids analysis was carried out using the gas chromatography method with a mass selective detector (GC/MSD) and using the database Library NIST14.L to identify the compounds.Results: Methyl esters fatty acids were identified from tocte (J. neotropica Diels) walnut using the GC–MS analytical method. The total lipid content of tocte walnuts seeds of plants cultivated in Ecuador was of 49.01% of the total lipid content on fresh weight. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GC–MS. Tocte walnut presents 5.05% of palmitic acid, 2.26% of stearic acid, 19.50% of oleic acid, 65.81% of linoleic acid, and 2.79% linolenic acid of the total content of fatty acids in tocte oil. Fatty acids content reported in this study were similar to the data reported for other walnuts seeds.Conclusions: Tocte seeds are a good source of monounsaturated and polyunsaturated fatty acids. Tocte oil content oleic acid and with a good content of ɷ6 α-linoleic and ɷ3 α-linolenic. Tocte walnut can help reduce risk cardiovascular diseases in Ecuador for their good composition of fatty acids.


Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2713 ◽  
Author(s):  
Mayakrishnan Prabakaran ◽  
Kyoung-Jin Lee ◽  
Yeonju An ◽  
Chang Kwon ◽  
Soyeon Kim ◽  
...  

Soybeans are low in saturated fat and a rich source of protein, dietary fiber, and isoflavone; however, their nutritional shelf life is yet to be established. This study evaluated the change in the stability and quality of fatty acids in raw and roasted soybean flour under different storage temperatures and durations. In both types of soybean flour, the fatty-acid content was the highest in the order of linoleic acid (18-carbon chain with two double bonds; C18:2), oleic acid (C18:1), palmitic acid (C16:0), linolenic acid (18:3), and stearic acid (C18:0), which represented 47%, 26%, 12%, 9%, and 4% of the total fatty-acid content, respectively. The major unsaturated fatty acids of raw soybean flour—oleic acid, linoleic acid, and linolenic acid—decreased by 30.0%, 94.4%, and 97.7%, and 38.0%, 94.8%, and 98.0% when stored in polyethylene and polypropylene film, respectively, after 48 weeks of storage under high-temperature conditions. These values were later increased due to hydrolysis. This study presents the changes in composition and content of two soybean flour types and the changes in quality and stability of fatty acids in response to storage temperature and duration. This study shows the influence of storage conditions and temperature on the nutritional quality which is least affected by packing material.


Author(s):  
Mária Vicianová ◽  
Ladislav Ducsay ◽  
Pavel Ryant ◽  
Marek Provazník ◽  
Alexandra Zapletalová ◽  
...  

The effect of phosphorus (P) and nitrogen (N) application on yield, oil and fatty acids content (especially oleic acid, linoleic acid and linolenic acid) in rapeseed was investigated in the field experiment. Also effect of weather conditions was evaluated. The polyfactorial trial was realized in experimental years 2013/2014 and 2014/2015 in terms of agricultural cooperative in Mojmírovce. The experiment was based on three variants of fertilization treatments by the block method in three replications. The size of each block was created by plots with size 600 m2. The first level of treatment 10 was non-fertilized control. The second level of treatment 2U was fertilized by nitrogen in dose 240 kg.ha-1. The third level of treatment 3P was fertilized by the same dose of nitrogen 240 kg.ha-1 and by phosphorus in dose 88 kg.ha-1. The highest average yield 3.9 t.ha-1 was achieved at treatment 3P, where phosphorus was applied. It means statistically significant yield increase by 30% compared to treatment 2U, where no phosphorus was not applied. There was statistically non-significant difference in oil content, in the range of treatments. The content of oleic acid fluctuated from 63.3% to 65.9% and the highest was reached at unfertilized control treatments in both experimental years. The linoleic acid content ranged from 20.3% to 21.2% and content of linolenic acid varied between 6.9% and 8.9%. Application of nitrogen and nitrogen + phosphorus high statistically significant decreased oleic acid content in both experimental years. Opposite effect was observed, where content of linoleic and linolenic acid was high statistically significant increased after nitrogen and nitrogen-phosphorus fertilization. Effect of unequal weather conditions and treatments of nitrogen and nitrogen-phosphorus nutrition can influence the percentage ratio of higher fatty acids composition.


2021 ◽  
Vol 910 (1) ◽  
pp. 012042
Author(s):  
Ali S. Hassoon ◽  
Jassim Jawad Jader Alnuaimi

Abstract Field experiment was carried out in the fields in Al-Mahaweel district of Babil Governorate during the agricultural season 2020-2021 to estimate the effect of biological fertilization on the content of some fatty acids in flax varieties seeds. A factorial experiment was applied according to a Randomized Complete Block Design (R.C.B.D) with three replications. The experiment included two factors, the first included eight varieties of flax (Indian, Giza11, Giza10, Sahka5, Sahka6, Giza8, Syrian Thorshansity72), The second factor, biological fertilization included four treatments: (control, bacterial fertilization, fungal fertilization and a bacterial-fungal mixture). The fatty acids (a-Linolenic acid, Oleic acid, Linoleic acid, Palmitic acid, Stearic acid) in seeds were determined using HPLC High-Performance Liquid Chromatography. The results showed that Sahka6 Variety was significantly superior on other varieties in seed content of (Oleic acid and Linoleic acid) amounted to (29.70, 28.39) %, respectively, while Indian Variety superiority in seed content of Palmitic acid and Stearic acid amounted to (21.28, 25.27)% respectively. Bacterial fertilizer + Mycorrhiza were significantly superior to the other fertilizer treatments in all indicators of study, and the interaction between a variety and biofertilization did not show any significant differences except for the seed content of (a-Linolenic acid Palmitic acid).


1992 ◽  
Vol 285 (2) ◽  
pp. 557-562 ◽  
Author(s):  
T Liang ◽  
S Liao

Human or rat microsomal 5 alpha-reductase activity, as measured by enzymic conversion of testosterone into 5 alpha-dihydrotestosterone or by binding of a competitive inhibitor, [3H]17 beta-NN-diethulcarbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one ([3H]4-MA) to the reductase, is inhibited by low concentrations (less than 10 microM) of certain polyunsaturated fatty acids. The relative inhibitory potencies of unsaturated fatty acids are, in decreasing order: gamma-linolenic acid greater than cis-4,7,10,13,16,19-docosahexaenoic acid = cis-6,9,12,15-octatetraenoic acid = arachidonic acid = alpha-linolenic acid greater than linoleic acid greater than palmitoleic acid greater than oleic acid greater than myristoleic acid. Other unsaturated fatty acids such as undecylenic acid, erucic acid and nervonic acid, are inactive. The methyl esters and alcohol analogues of these compounds, glycerols, phospholipids, saturated fatty acids, retinoids and carotenes were inactive even at 0.2 mM. The results of the binding assay and the enzymic assay correlated well except for elaidic acid and linolelaidic acid, the trans isomers of oleic acid and linoleic acid respectively, which were much less active than their cis isomers in the binding assay but were as potent in the enzymic assay. gamma-Linolenic acid had no effect on the activities of two other rat liver microsomal enzymes: NADH:menadione reductase and glucuronosyl transferase. gamma-Linolenic acid, the most potent inhibitor tested, decreased the Vmax. and increased Km values of substrates, NADPH and testosterone, and promoted dissociation of [3H]4-MA from the microsomal reductase. gamma-Linolenic acid, but not the corresponding saturated fatty acid (stearic acid), inhibited the 5 alpha-reductase activity, but not the 17 beta-dehydrogenase activity, of human prostate cancer cells in culture. These results suggest that unsaturated fatty acids may play an important role in regulating androgen action in target cells.


2012 ◽  
Vol 66 (2) ◽  
pp. 207-209 ◽  
Author(s):  
Boris Pejin ◽  
Ljubodrag Vujisic ◽  
Marko Sabovljevic ◽  
Vele Tesevic ◽  
Vlatka Vajs

The fatty acid composition of the moss species Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) and Hypnum andoi A.J.E. Sm. (Hypnaceae) collected in winter time were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) as a contribution to their chemistry. Eight fatty acids were identified in the chloroform/methanol extract 1:1 of A. undulatum (linoleic acid 26.80%, palmitic acid 22.17%, ?-linolenic acid 20.50%, oleic acid 18.49%, arachidonic acid 6.21%, stearic acid 3.34%, cis-5,8,11,14,17-eicosapentaenoic acid 1.52% and behenic acid 1.01%), while six fatty acids were found in the same type of extract of H. andoi (palmitic acid 63.48%, erucic acid 12.38%, stearic acid 8.08%, behenic acid 6.26%, lignoceric acid 5.16% and arachidic acid 4.64%). According to this study, the moss A. undulatum can be considered as a good source of both essential fatty acids for humans (linoleic acid and ?-linolenic acid) during the winter.


1986 ◽  
Vol 59 (5) ◽  
pp. 800-808 ◽  
Author(s):  
James M. Sloan ◽  
Michael J. Maghochetti ◽  
Walter X. Zukas

Abstract An effort to characterize the reversion process of guayule rubber when naturally-occurring guayule resin components are present has shown that these components act as a reversion-retarding material. The amount of reversion resistance varies as a function of temperature, concentration, and type of fatty acid. Of the three fatty acids used, linoleic acid, stearic acid, and oleic acid, linoleic acid performed the best for reversion resistance, followed by stearic acid, then oleic acid. When the temperature was increased 10°C, an increase of 15% reversion was observed. This held true for the three temperatures studied. In addition, the amount of reversion improvement upon addition was 20% reversion. In the case of curing at 150°C, this resulted in 0% reversion. The 20% resistance improvment was consistent for the 3 temperatures studied.


2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Rashmi Kumari ◽  
Gopal Rao Mallavarapu ◽  
Vinod Kumar Jain ◽  
Sushil Kumar

Fatty oils of the seeds of Cleome viscosa accessions from Delhi, Jaipur, Faridabad, Surajkund and Hyderabad were methylated and analyzed by GC and GC-MS. The major fatty acids, identified as their methyl esters, of the oils from these five locations were palmitic acid (10.2-13.4%), stearic acid (7.2-10.2%), oleic acid (16.9-27.1%) and linoleic acid (47.0-61.1%). In addition, palmitoleic acid, octadec-(11 E)-enoicacid, arachidic acid, eicosa-(11 Z)-enoic acid, linolenic acid, heneicosanoic acid, behenic acid, lignoceric acid, pentacosanoic acid, hexacosanoic acid, 12-oxo-stearic acid, and the alkanes tetracosane, pentacosane, hexacosane, heptacosane, octacosane, nonacosane, triocontane, hentriacontane and dotriacontane, were also identified as minor and trace constituents in some of these oils.


1968 ◽  
Vol 22 (4) ◽  
pp. 681-688 ◽  
Author(s):  
J. H. Moore ◽  
R. C. Noble ◽  
W. Steele

1. In Expt 1, four adult wether sheep were given diets of hay or dried grass in an alternating sequence of feeding treatments. Each treatment period lasted for 20 days and blood samples were taken from the sheep on the last day of each period. When the diet of hay (linoleic: linolenic acid ratio 1.40) was replaced by one of dried grass (linoleic: linolenic acid ratio 0.28), there was an increase in the concentration of linolenic acid and a decrease in the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. There was an increase in the concentration of stearic acid and a decrease in the concentration of palmitic acid in the plasma triglycerides. Dietary change did not affect the composition of the plasma unesterified fatty acids.2. In Expt 2, two adult wether sheep, each with a rumen fistula, were given daily intraruminal infusions of 60 g of ‘linolenic’ or ‘linoleic’ acids (both about 70% pure) over a period of 5 days. The infusion treatments were then reversed. Blood samples were taken at the end of each infusion period. Intraruminal infusions of ‘linolenic’ acid increased the concentration of linolenic acid in the plasma cholesteryl esters and phospholipids and increased the concentration of stearic acid in the plasma triglycerides. The infusions of ‘linoleic’ acid increased the concentration of linoleic acid in the plasma cholesteryl esters and phospholipids. No appreciable changes were observed in the composition of the plasma unesterified fatty acids.


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