Utilization of specific primers in legume allergens based polymorphism screening

2021 ◽  
Vol 13 (2) ◽  
pp. 12-21
Author(s):  
Lucia Klongová ◽  
Adam Kováčik ◽  
Lucia Urbanová ◽  
Matúš Kyseľ ◽  
Eva Ivanišová ◽  
...  
Keyword(s):  
Plant Food ◽  
Specific Primers ◽  
Genomic Variability ◽  
Research Activities ◽  
Genomic Screening ◽  
The World ◽  
Different Types ◽  
Immunological Interactions ◽  
Bioinformatic Approach ◽  
Polymorphism Screening

Different types of allergies became a part of life of many people around the world. The research activities connecting to allergens are actually not oriented only for protein and immunological interactions, but to the genomic and transcriptomic background of them, too. Analysis and description of genomic variability of allergens in plant food resources will help to manage the allergen based strategies in the future. Here, the bioinformatic approach was used to develop and validate the specific primers for genomic screening of polymorphism of profilins (Profilin Based Amplicon Polymorphism; PBAP) and vicilins (Vicilin Based Amplicon Polymorphism; VBAP) among the legumes. The alignment of existing public databases data for these allergens in the group of legumes was performed. Subsequently, specific primers were designed and their ability to generate polymorphic amplicons were tested for three legumes – bean, lentil and chickpeas. In all cases, amplicons were generated and polymorphism was detected in all three species for profilin as well as for vicilin.

scholarly journals Detection of Urovirulence Genes (eae,E-hly,α-hly) of Uropathogenic Escherichia coli by Specific PCR

2010 ◽  
Vol 4 (1) ◽  
pp. 44-54
Author(s):  
Rawa’a Al-Chalabi ◽  
Ayad Al –Ubaidy ◽  
Muneera Al- Ibadi
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Health Problem ◽  
Intestinal Flora ◽  
Uropathogenic Escherichia Coli ◽  
Specific Primers ◽  
Specific Pcr ◽  
Erythrocyte Lysis ◽  
The World ◽  
Different Types

Urinary tracts infection remains a common troublesome health problem in the world. Although E.coli is normal intestinal flora but considered as the main important opportunistic active uropathogene because of it’s pathogenesity which referred to it’s different virulence factors like hemolysin, biofilm, enterotoxins, shiga like toxins, cytotoxic necrotizing factor and others. Seventy seven E.coli isolates which isolated from 125 midstream urine samples of patients suffering from different types of UTIs. The results showed that 44 isolates 57% were produced hemolysin. These isolates were differed in the efficiency of erythrocyte lysis because which depend on type of hemolysin and source of blood. Seventy two isolates 90.9% were formed biofilm with variety in thickness of biofilm layer. Each isolate produce hemolysin also formed biofilm. Presence of genes encoded for virulence traits of UPEC (hemolysin, biofilm) were examined by PCR with specific primers. The results showed that the percentage of α-hly gene encoded for α-hemolysin 50% while percentage of eae gene encoded for intimin and E-hly gene encoded for enterohemolysin were 40% and 20% respectively.

Knowledge and Awareness of Dengue Fever

2018 ◽  
pp. 219-222
Author(s):  
Y. Arockia Suganthi ◽  
Chitra K. ◽  
J. Magelin Mary
Keyword(s):  
Dengue Fever ◽  
Prevention And Control ◽  
Control Measures ◽  
Infection Transmission ◽  
Standard Guideline ◽  
The World ◽  
Different Types ◽  
Prevention And Control Measures ◽  
Dengue Prevention ◽  
And Control

Dengue fever is a painful mosquito-borne infection caused by different types of virus in various localities of the world. There is no particular medicine or vaccine to treat person suffering from dengue fever. Dengue viruses are transmitted by the bite of female Aedes (Ae) mosquitoes. Dengue fever viruses are mainly transmitted by Aedes which can be active in tropical or subtropical climates. Aedes Aegypti is the key step to avoid infection transmission to save millions of people in all over the world. This paper provides a standard guideline in the planning of dengue prevention and control measures. At the same time gives the priorities including clinical management and hospitalized dengue patients have to address essentially.

Front-line Healthcare Workers in COVID-19: The Way from Elbow-Bump Greeting to Closing Body Bags

2020 ◽  
Author(s):  
Stefan Bittmann
Keyword(s):  
Health Service ◽  
Healthcare Workers ◽  
Well Being ◽  
The United States ◽  
Angiotensin Ii Receptor ◽  
Front Line ◽  
Resource Center ◽  
Research Activities ◽  
The World ◽  
The Usa

COVID-19 is a serious coronavirus disease that is spreading all over the world. As of the date of this publication, 2.834.134 people have been infected with COVID-19 and 197.924 deaths have been recorded in 185 countries (John Hopkins Corona Resource Center, 25th April 2020) [1]. This overwhelming mortality rate requires intensive research activities around the world. To date, the number of deaths per day in the United States is still killing, indicating an uncontrollable state of infection spread. SARS-CoV-2 binds to the angiotensin II receptor in various tissues of the human body, particularly in the oral cavity and tongue. SARS-CoV-2 requires the cheerful TMPRSS2 to activate this inertia. SARS-CoV-2 uses the ACE2 receptor as a gateway to the lungs. The SARS-CoV-2 virus binds with the spike protein to the ACE2 receptor. COVID-19 is more common among African Americans in the USA (Science 10th April 2020). The comfort and the emotional loading capacity of the employees in the health service are key components for the maintenance of the essential health services during the outbreak of the COVID-19 virus (Coronavirus) [2,3]. Hence, it will be important to anticipate the charges linked with this work and to release support for employees in the health service. The supervision and assessment of the psychic health and the well-being of the employees in the health service will be important, just as the efforts to guarantee a successful reunion with colleagues if they are infected.

Analysis of babA, cagE and cagA Genes in Helicobacter pylori from Upper Gastric Patients in the North of Iran

2019 ◽  
Vol 19 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Saba Fakhrieh Asl ◽  
Mehrnaz Pourvahedi ◽  
Ali Mojtahedi ◽  
Mohammad Shenagari
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Diseases ◽  
Specific Primers ◽  
The North ◽  
Different Types ◽  
North Of Iran ◽  
Gastric Biopsies ◽  
Pcr Method ◽  
Non Ulcer Dyspepsia ◽  
H Pylori

Objective:Helicobacter pylori is a Gram-negative bacterium which has a serious effect on up to half of the world’s population and has been related to different gastric diseases. The goal of this study was to assess the frequency of babA, cagE and cagA genotypes among H. pylori strains isolated from gastric biopsies of endoscopic patients in the north of Iran.Methods:The present study was performed on 90 strains of H. pylori isolated from patients with gastric diseases (Gastric ulcer (GU), Duodenal ulcer (DU), Gastritis (G), Non-ulcer dyspepsia (NUD) and Gastric adenocarcinoma (GC)). DNA was extracted from all isolated strains and PCR method was performed to detect the prevalence of babA2, cagE and cagA genes using specific primers.Results:Among 90 samples of H. pylori, babA2, cagE, and cagA genes were detected in 42.2%, 30% and 82.2% of strains respectively. The statistical analysis showed that the prevalence of cagA gene in GU, G, DU, and NUD was significantly higher than other genes. Moreover, cagA, and babA2 genes were significantly more prevalent in GC patients compared to cagE gene. Our isolates exhibited 8 distinct arrangements of virulence patterns. The occurrence of cagA (35.6%) was the most prevalent pattern followed by cagA/babA2 (20%) and cagA/babA2/cagE (14.4%).Conclusion:In summary, as first report from Guilan province in the north of Iran, we showed significant association between the presence of babA2, cagE, and cagA genes in different types of gastric disorders.

Platón, Einstein y el triunfo de la imaginatividad cosmológica

1998 ◽  
pp. 87-98
Author(s):  
T. M. Robinson
Keyword(s):  
Dynamic Model ◽  
Striking Similarity ◽  
Empirical World ◽  
The World ◽  
Modern Cosmology ◽  
Different Types ◽  
Physical Objects ◽  
Changing World

This article argues the following five claims: 1. Plato’s description of the origins of cosmos in the Timaeus is not a myth, nor something unlikely: when he called it an eikos mythos or eikos logos, he meant a likely or trustworthy account on this very subject. 2. Among the details in this account, the following are prominent and surprising: a) the world was fashioned in time, in that precise point that was the beginning of time; b) several kinds of duration can be distinguished in cosmology (mainly eternity, sempiternity, perpetuity and time); and c) space is an entity characterized by movement and tension. 3. In the Statesman, Plato repeats much the same thing, adding this time the strange notion that the universe’s circular movement is periodically reversed. 4. In spite of the important differences in detail, there is a striking similarity between Plato’s account of the origins of the world and the explanation adopted by much of modern cosmology. 5. What Plato shares with so many instances of recent thought is here termed “cosmological imaginativity”. A first section of the paper deals exclusively with the Timaeus. Claims 1 and 2a are supported by a revision of the meanings of mythos and logos, followed by brief reference and discussion of the argument at Timaeus 27d, leading to the conclusion that Plato affirms that the ever-changing world has indeed had a beginning in time. Claim 2b describes five different types of duration, corresponding to Forms, the Demiurge, Space, the [empirical] world and its contents, physical objects. The second section is concerned with the myth in the Statesman, discussing it as a parallel and describing its peculiar turn to the Timaeus’ cosmology and cosmogony, a complex spheric and dynamic model. After digressing into some important ideas in modern cosmology, touching especially on affinities of some of Einstein’s ideas with of Plato’s own, the paper closes with a discussion of cosmological imaginativity, oriented to recover and recognize fully Plato’s greatness as a cosmologist.

Effective Altruism

2019 ◽  
Keyword(s):  
Animal Welfare ◽  
Poverty Alleviation ◽  
Future Generations ◽  
Global Poverty ◽  
Global Community ◽  
Helping Others ◽  
The World ◽  
Different Types ◽  
Effective Altruism

The effective altruism movement consists of a growing global community of people who organize significant parts of their lives around two key ideas, represented in its name. Altruism: If we use a significant portion of the resources in our possession—whether money, time, or talents—with a view to helping others, we can improve the world considerably. Effectiveness: When we do put such resources to altruistic use, it is crucial to focus on how much good this or that intervention is reasonably expected to do per unit of resource expended (for example, per dollar donated). While global poverty is a widely used case study in introducing and motivating effective altruism, if the ultimate aim is to do the most good one can with the resources expended, it is far from obvious that global poverty alleviation is highest priority cause area. In addition to ranking possible poverty-alleviation interventions against one another, we can also try to rank interventions aimed at very different types of outcome against one another. This includes, for example, interventions focusing on animal welfare or future generations. The scale and organization of the effective altruism movement encourage careful dialogue on questions that have perhaps long been there, throwing them into new and sharper relief, and giving rise to previously unnoticed questions. In the present volume, the first of its kind, a group of internationally recognized philosophers, economists, and political theorists contribute in-depth explorations of issues that arise once one takes seriously the twin ideas of altruistic commitment and effectiveness.

scholarly journals Sweet Corn Research around the World 2015–2020

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 534
Author(s):  
Pedro Revilla ◽  
Calli M. Anibas ◽  
William F. Tracy
Keyword(s):  
Sweet Corn ◽  
Starch Content ◽  
Starch Synthesis ◽  
Environmental Care ◽  
Research Activities ◽  
The Us ◽  
The World ◽  
The Usa ◽  
Basic Biology ◽  
Endosperm Starch

Modern sweet corn is distinguished from other vegetable corns by the presence of one or more recessive alleles within the maize endosperm starch synthesis pathway. This results in reduced starch content and increased sugar concentration when consumed fresh. Fresh sweet corn originated in the USA and has since been introduced in countries around the World with increasing popularity as a favored vegetable choice. Several reviews have been published recently on endosperm genetics, breeding, and physiology that focus on the basic biology and uses in the US. However, new questions concerning sustainability, environmental care, and climate change, along with the introduction of sweet corn in other countries have produced a variety of new uses and research activities. This review is a summary of the sweet corn research published during the five years preceding 2021.

Comprehensive Effects of Short-Term Rental Platforms Across Hotel Types in U.S. and International Destinations

2021 ◽  
pp. 193896552110335
Author(s):  
John W. O’Neill ◽  
Jihwan Yeon
Keyword(s):  
Service Providers ◽  
Comprehensive Analysis ◽  
Competitive Market ◽  
Substitution Effects ◽  
Short Term ◽  
Hotel Performance ◽  
The World ◽  
Different Types ◽  
Location Type ◽  
The Impact

In recent years, short-term rental platforms in the lodging sector, including Airbnb, VRBO, and HomeAway, have received extensive attention and emerged as potentially alternative suppliers of services traditionally provided by established commercial accommodation providers, that is, hotels. Short-term rentals have dramatically increased the available supply of rooms for visitors to multiple international destinations, potentially siphoning demand away from hotels to short-term rental businesses. In a competitive market, an increase in supply with constant demand would negatively influence incumbent service providers. In this article, we examine the substitution effects of short-term rental supply on hotel performance in different cities around the world. Specifically, we comprehensively investigate the substitution effects of short-term rental supply on hotel performance based on hotel class, location type, and region. Furthermore, we segment the short-term rental supply based on its types of accommodations, that is, shared rooms, private rooms, and entire homes, and both examine and quantify the differential effects of these types of short-term rentals on different types of hotels. This study offers a comprehensive analysis regarding the impact of multiple short-term rental platforms on hotel performance and offers both conceptual and practical insights regarding the nature and extent of the effects that were identified.

scholarly journals Withdrawal Properties of Self-Tapping Screws in Japanese larch (Larix kaempferi (Lamb.) Carr.) Cross Laminated Timber

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 524
Author(s):  
Junhua Xu ◽  
Shuangbao Zhang ◽  
Guofang Wu ◽  
Yingchun Gong ◽  
Haiqing Ren
Keyword(s):  
Regression Model ◽  
Engineering Design ◽  
Wood Species ◽  
Japanese Larch ◽  
Effective Length ◽  
Larix Kaempferi ◽  
Cross Laminated Timber ◽  
The World ◽  
Different Types ◽  
Length Increment

With the increasing popularity of cross-laminated timber (CLT) constructions around the world, there have been attempts to produce CLT using local wood species in different countries, such as Japanese larch (Larix kaempferi (Lamb.) Carr.) in China. Thus, the need to investigate the connection performance also increases to support the design and construction of CLT buildings using local wood species. In this study, the withdrawal properties of three different types of self-tapping screws (STS), with a diameter of 6 mm, 8 mm, and 11 mm, were tested with Japanese larch CLT. The results revealed that the withdrawal strength of STS increased with increasing density and effective length, but decreased with an increasing diameter. With a density increment of 0.05 g/cm3, the withdrawal strength increased by an average of 9.4%. With an effective length increment of 24 mm, the withdrawal strength increased by an average of 1.4%. An empirical regression model was adopted to predict the withdrawal strength of Japanese larch CLT based on the results, which can be used for potential engineering design of CLT connections using STS.

scholarly journals The Effects of Urban Natural Environments on Preference and Self-Reported Psychological Restoration of the Elderly

2021 ◽  
Vol 18 (2) ◽  
pp. 509
Author(s):  
Ling Qiu ◽  
Qujing Chen ◽  
Tian Gao
Keyword(s):  
Green Space ◽  
Environmental Design ◽  
The Elderly ◽  
Urban Environments ◽  
Green Spaces ◽  
Photo Elicitation ◽  
Natural Environments ◽  
The World ◽  
Different Types ◽  
Aging Populations

The world is facing the challenge of aging populations. Urban natural environments, including green spaces and blue spaces, have been demonstrated to have great benefits to the mental restoration of the elderly. However, the study of the specific characteristics of urban environments that are popular and the most restorative for the elderly is still lacking. Photo elicitation as visual stimuli was utilized to explore the differences in preference and psychological restoration of the elderly through the perception of the eight perceived sensory dimensions (PSDs) in different types of urban environments. The results showed that: (1) The respondents had different perceptions of the eight PSDs in the different urban natural environments. Blue space and partly-closed green space were more preferred by the elderly, and also had more psychological restorative effects on the elderly. (2) There was no significant correlation between the number of highly perceived PSDs and preference, as well as between the number of highly perceived PSDs and psychological restoration. However, there was a significant correlation between preference and psychological restoration. (3) Partly-closed green space with more Serene and Refuge qualities, and blue space with more Serene, Refuge and Prospect properties were optimal characteristics for psychological restoration of the elderly. In addition, open green space with more Prospect, Serene and Social qualities, and closed green space with more Space, Refuge and less Nature properties could also increase psychological restoration of older adults. These findings can provide useful guidelines for restorative environmental design for the elderly in the future.

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