Inhibitory Effect of Luteolin Liposome Solution by Animal Model for Atopic Dermatitis in NC/Nga Mice

AbstractCold atmospheric plasma (CAP) has been incorporated into various fields, including promotion of cutaneous wound healing. Atopic dermatitis (AD) is a chronic cutaneous condition characterized by inflammation-induced skin wounds and impaired skin barrier function. To investigate whether CAP may improve AD using an animal model. Dermatophagoides farinae extracts (DFE)-induced murine models of AD were used in this study. The plasma-treated group received a total of 6 CAP treatments during 2 weeks, while the control group did not receive any treatment. Differences in dermatitis severity, transepidermal water loss (TEWL), serum level of immunoglobulin (Ig) E and epidermal thickness were evaluated in both groups. The dermatitis severity was significantly improved by CAP treatment. TEWL was lower in the plasma-treated group compared with the non-treated control group. Serum Ig E dropped significantly after treatment with CAP. Difference in epidermal thickness of the ear skin was not significant between the plasma-treated and non-treated groups. Localized treatment of AD with CAP decreases dermatitis severity, TEWL, and serum Ig E level. These results show CAP’s potentials as a novel therapeutic modality for AD.

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Nephropathy Induced by Intramuscularly Administered Glycerol and Contrast Media in Rats

Acta Radiologica ◽

10.1177/028418518903000220 ◽

1989 ◽

Vol 30 (2) ◽

pp. 217-222 ◽

Cited By ~ 3

Author(s):

H. S. Thomsen ◽

S. Larsen ◽

L. Hemmingsen ◽

J. Holm ◽

P. Skaarup

Keyword(s):

Animal Model ◽

Contrast Media ◽

Light Microscopy ◽

Cell Response ◽

Wistar Rats ◽

Inhibitory Effect ◽

Tubular Dysfunction ◽

Chemical Analyses ◽

Round Cell ◽

Histologic Finding

Urine profiles (albumin, glucose, NAG, LDH, GGT, sodium, and phosphate) were followed for 14 days after intravenous injection of either diatrizoate, iohexol, ioxilan, or saline in 24 Wistar rats with a glomerular and tubular dysfunction induced by intramuscularly (i.m.) administered glycerol. Another 6 rats exposed to neither glycerol nor contrast media served as controls. The effect of ioxilan and saline on the albumin excretion was similar, whereas diatrizoate and iohexol increased it significantly. The contrast media had no further inhibitory effect on the reabsorption of glucose. Iohexol caused significantly increased excretion of all three enzymes, ioxilan of NAG and LDH, whereas diatrizoate only increased the excretion of LDH. The sodium excretion was further increased by ioxilan and diatrizoate, whereas none of the contrast media affected the phosphaturia. Both ioxilan and iohexol caused a round cell response around the tubules shown by light microscopy whereas diatrizoate caused no further changes. It is concluded that diatrizoate and iohexol increase glomerular dysfunction induced by glycerol i.m.; all three contrast media cause some further increase in the tubular dysfunction. Neither diatrizoate, iohexol nor ioxilan prolong nephropathy induced by glycerol i.m. determined by the chemical analyses. The histologic finding indicates a direct toxic effect of non-ionic low osmolar contrast media in this animal model of nephropathy.

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Sex differences in the antithrombotic effects of aspirin

Blood ◽

10.1182/blood.v52.5.1073.1073 ◽

1978 ◽

Vol 52 (5) ◽

pp. 1073-1076 ◽

Cited By ~ 55

Author(s):

JG Kelton ◽

J Hirsh ◽

CJ Carter ◽

MR Buchanan

Keyword(s):

Clinical Trials ◽

Animal Model ◽

Arterial Thrombosis ◽

Sodium Salicylate ◽

Inhibitory Effect ◽

Prostaglandin Synthesis ◽

Antithrombotic Effect ◽

Antithrombotic Agent ◽

Male And Female ◽

Antithrombotic Effects

Abstract Aspirin inhibits platelet function by acetylating platelet cyclooxygenase. Recent clinical trials indicate that aspirin is a promising antithrombotic agent against both venous and arterial thrombosis, but somewhat surprisingly this protective effect appears to be limited to males. To examine the potential sex-related differences in response to aspirin, we developed an animal model for quantitating fibrin accretion into an injury-induced thrombus and used it to study the effects of aspirin on thrombus size in male and female rabbits. Platelet prostaglandin synthesis was estimated by assay of platelet malondialdehyde and was significantly decreased in both male and female rabbits following treatment with 10 mg/kg aspirin (p less than 0.001). This inhibitory effect was not different for platelets from male and female rabbits. Thrombus size was significantly decreased in aspirin- treated male rabbits when compared to controls (p less than 0.05), but this aspirin effect was not apparent in female rabbits or rabbits of either sex treated with 10 mg/kg sodium salicylate. These findings support the results of clinical trials that were obtained by retrospective subgroup analysis. The reason for the sex difference is not known, but the findings raise an important issue in relationship to this mechanism of the antithrombotic effect of aspirin.

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Altered mRNA and protein expression of filaggrin in the skin of a canine animal model for atopic dermatitis

Veterinary Dermatology ◽

10.1111/vde.12031 ◽

2013 ◽

Vol 24 (3) ◽

pp. 329-e73 ◽

Cited By ~ 20

Author(s):

Domenico Santoro ◽

Rosanna Marsella ◽

Kim Ahrens ◽

Thomas K. Graves ◽

David Bunick

Keyword(s):

Animal Model ◽

Atopic Dermatitis ◽

Protein Expression ◽

Mrna And Protein Expression

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Inhibitory Effect of Centella asiatica Extract on DNCB-Induced Atopic Dermatitis in HaCaT Cells and BALB/c Mice

Nutrients ◽

10.3390/nu12020411 ◽

2020 ◽

Vol 12 (2) ◽

pp. 411 ◽

Cited By ~ 4

Author(s):

Yonghyeon Lee ◽

Hyeon Kyeong Choi ◽

Kaudjhis Patrick Ulrich N’deh ◽

Young-Jin Choi ◽

Meiqi Fan ◽

...

Keyword(s):

Atopic Dermatitis ◽

Allergic Inflammation ◽

Centella Asiatica ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Interferon Γ ◽

Dependent Manner ◽

Hacat Cells ◽

Signaling Mechanism ◽

Tnf Α

Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the Centella asiatica ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm2, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.

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Flaky Tail Mouse as a Novel Animal Model of Atopic Dermatitis: Possible Roles of Filaggrin in the Development of Atopic Dermatitis

Atopic Dermatitis - Disease Etiology and Clinical Management ◽

10.5772/25609 ◽

2012 ◽

Author(s):

Catharina Sagita ◽

Kenji Kabashim

Keyword(s):

Animal Model ◽

Atopic Dermatitis

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The inhibitory effect of naringenin on atopic dermatitis induced by DNFB in NC/Nga mice

Life Sciences ◽

10.1016/j.lfs.2013.07.027 ◽

2013 ◽

Vol 93 (15) ◽

pp. 516-524 ◽

Cited By ~ 18

Author(s):

Tae-Ho Kim ◽

Gun-Dong Kim ◽

Hyun-Jong Ahn ◽

Jeong-Je Cho ◽

Yong Seek Park ◽

...

Keyword(s):

Atopic Dermatitis ◽

Inhibitory Effect

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Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin

Journal of Experimental Medicine ◽

10.1084/jem.20041503 ◽

2005 ◽

Vol 202 (4) ◽

pp. 541-549 ◽

Cited By ~ 413

Author(s):

Jane Yoo ◽

Miyuki Omori ◽

Dora Gyarmati ◽

Baohua Zhou ◽

Theingi Aye ◽

...

Keyword(s):

Animal Model ◽

Atopic Dermatitis ◽

Transgenic Mice ◽

Elevated Serum ◽

Allergic Inflammation ◽

Disease Process ◽

Allergic Diseases ◽

Serum Levels ◽

Thymic Stromal Lymphopoietin ◽

Common Disease

The cytokine thymic stromal lymphopoietin (TSLP) has recently been implicated in the pathogenesis of atopic dermatitis (AD) and other allergic diseases in humans. To further characterize its role in this disease process, transgenic mice were generated that express a keratinocyte-specific, tetracycline-inducible TSLP transgene. Skin-specific overexpression of TSLP resulted in an AD-like phenotype, with the development of eczematous lesions containing inflammatory dermal cellular infiltrates, a dramatic increase in Th2 CD4+ T cells expressing cutaneous homing receptors, and elevated serum levels of IgE. These transgenic mice demonstrate that TSLP can initiate a cascade of allergic inflammation in the skin and provide a valuable animal model for future study of this common disease.

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Mouse model of DNCB-induced atopic dermatitis

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v12i2.31950 ◽

2017 ◽

Vol 12 (2) ◽

pp. 30

Author(s):

Alshammari Fanar Hamad ◽

Jong-Hun Han ◽

Irfan Ahmad Rather

Keyword(s):

Animal Model ◽

Atopic Dermatitis ◽

Video Clip ◽

Skin Inflammation ◽

New Drugs ◽

Dorsal Skin ◽

Reference Drug ◽

Allergic Skin ◽

Full Screen ◽

Selection Of

Atopic dermatitis is a skin disease characterized by allergic skin inflammation, redness and itching. The animal model is necessary to find out new drugs. The DNCB-induced animal model of atopic dermatitis includes the following steps: 1) Selection of animals; 2) Shaving of dorsal skin; 3) Applying DNCB once in 24 hours for three days; 4) Monitoring the development of atopy on day 4 post DNCB application. Further, the efficacy of reference drug can be determined by applying on the atopy skin, depends on the nature and aim of the work.Video Clip of Methodology: 8 min <a href="https://www.youtube.com/v/XbFt1bOnulc">Full Screen</a> <a href="https://www.youtube.com/watch?v=XbFt1bOnulc">If Failed</a>

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