scholarly journals Adequacy of the accelerated aging test for soybean seeds

2021 ◽  
Vol 17 (4) ◽  
pp. 1-13
Author(s):  
Anna dos Santos Suñé ◽  
Andréia da Silva Almeida ◽  
Josiane Cantuária Figueiredo ◽  
Bruna Barreto dos Reis ◽  
Camila de Avila Nunes ◽  
...  

Seed companies use vigor tests in their internal seed quality control procedures. Therefore, it is necessary to choose effective methods to obtain quick responses for making decisions related to the handling, disposal and marketing of seed lots. Thus, the objective of this work was to adapt a methodology for the accelerated aging test in soybean seeds. Sixty-nine lots were used and the experimental design used was completely randomized, with four replications. For the initial characterization of the lots, water content was determined, germination test and field emergence. The soybean seed lots selected based on the initial characterization, were submitted to the standard accelerated aging methodology and twenty-one adapted methodologies. After the physiological characterization of the 69 lots, 24 lots with similar germination with different levels of vigor were selected. For adapted methodologies that used only distilled water inside the gerbox box, there was an increase in the water content of the seeds of all analyzed lots. However, all methodologies with the use of saline solution had alower water content. The adapted methodologies N (pre-treatment in forced air oven at 35-40°C for two hours, BOD 42°C for 24 hours, using saline) and S (pre-treatment in forced air oven at 35-40°C for two hours, BOD 45°C for 12 hours, with the use of distilled water) are the most suitable for use in the accelerated aging test for soybean crops, as they present relevant results in a shorter evaluation period.

2021 ◽  
Vol 37 ◽  
pp. e37087
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Ítala Menegon Castilho ◽  
Franciele Caixeta ◽  
Nilvanira Donizete Tebaldi ◽  
Pâmela Gomes Nakada Freitas

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.


2013 ◽  
Vol 35 (3) ◽  
pp. 284-291
Author(s):  
Maria Angélica Lodo ◽  
Magnólia de Mendonça Lopes ◽  
Bruna Resende Ribeiro Soares ◽  
Roberval Daiton Vieira

The efficiency of vigor tests in assessing the physiological potential of seeds depends on their standardization for different species. In this context, the research aimed to study specific methodologies to evaluate the vigor of spinach seeds (Tetragonia tetragonoides). For this purpose, five lots of spinach seed cv. New Zealand were submitted to germination, first count of germination, seedling emergence in the field, accelerated aging (with and without use of saturated solution of NaCl at 41and 45 ºC for 24,48 and 72 hours), controlled deterioration (18,21 and 24% seed water content adjustments for 24 hours at 45 ºC) and electrical conductivity tests with variations inwater volume (25, 50 and 75 mL), seed quantity (25 and 50) and soaking period (1, 2, 4, 8,12, 16 and 24 hours) at 25 ºC. The first count test allowed obtaining preliminary information about seed vigor, and other tests in general showed similarity with seedling emergence in the field. Thus, it was concluded that traditional accelerated aging test 24 h /41 ºC and accelerated aging test with saturated salt solution for 24 h at 41 ºC and 45 ºC, and controlled deterioration test with 21% seed water content / 24 h / 45 ºC were all efficient for evaluating the physiological potential of spinach seeds.


2018 ◽  
Vol 11 (1) ◽  
pp. 376
Author(s):  
Andréia K. Suzukawa ◽  
Giovanna E. G. Mariucci ◽  
Lucas C. Pereira ◽  
Alessandro L. Braccini ◽  
Raissa M. Ponce ◽  
...  

Industrial seed treatment assures uniform coverage of seeds with insecticides, fungicides, micronutrients and bioestimulant at precise dose, however often resulting in higher slurry volume. Furthermore, seeds are coated long periods of time prior sowing and may influence germination and vigor. Thus, the aim of this work was to evaluate the influence of seven industrial seed treatments and their respective slurry volumes on the physiological potential of soybean seeds at 0, 15, 30, 45, 60 and 90 days of storage. In each storage period, the variables germination, accelerated aging test and emergency speed index were evaluated and studied in the repeated measures in time model. The products used were: fungicide (thiabendazole, fludioxonil and mefenoxam), insecticide (thiametoxam), insecticide/nematicide (abamectin), micronutrients (cobalt and molybdenum), bioregulator, drying powder and polymer. High volumes of seed-coating mixtures reduce the physiological quality of soybean seeds over storage. However, the slurry composition also influenced on the maintenance of the seed germination and vigor throughout the storage.


2018 ◽  
Vol 106 (4) ◽  
pp. 401
Author(s):  
Nadir Kerroum ◽  
Brahim Nouibat ◽  
Azzedine Benyahia ◽  
Ali Redjem

This article describes a study conducted to determine the ideal both traditional and stabilized composition of adobe as a building material, and to further refine an appropriate choice of coating composition for extended durability of these constructions in adobe: choose the suitable coating that fits the most with its substrate. The constructions in Z’gueg El Hadjadj [Laghouat town, Algeria] consist of adobe walls but also plaster that serves as a coating for these brick walls. The degradation phenomena of the building are considered in the adobe-coated couple, and consequently in the suitability of their compositions. The compatibility of plasters with adobes remains a major factor in the restoration and conservation of earthen constructions. This paper examines the results of the experimental characterization of the behavior of coated bricks, which consists of studying in the laboratory the water resistance: the drying-wetting test and the capillary rise test, as well as the complementary accelerated aging test: the abrasion test. These tests will be supplemented by another test, that of the adhesion, to see the compatibility of the coatings with their supports. The results, compared below, led to the conclusion that for capillary absorption and adhesion tests, a homogeneous coating in 3 layers could give more conclusive results. The drying-wetting test is recommended especially for bulk and surface treated samples.


2016 ◽  
Vol 38 (3) ◽  
pp. 233-240 ◽  
Author(s):  
Karina Laís Leite Sarath ◽  
André Luís Duarte Goneli ◽  
Cesar Pedro Hartmann Filho ◽  
Tathiana Elisa Masetto ◽  
Guilherme Cardoso Oba

Abstract: This study was performed to evaluate the immediate and latent effects of drying air temperature on the physiological potential of peanuts (Arachis hypogaea L.). The fruits were harvested at the physiological maturity stage, with 36 ± 2% water content (w.b.), and dried at temperatures of 40, 50, 60 and 70 ºC, until 7 ± 1% (w.b.). Thereafter, the fruits were stored in an environment with non-controlled temperature and relative humidity for 150 days, and the water content, germination and vigor of seeds were immediately determined, as well as at every 30 days thereafter, through the first count, and the tetrazolium test, accelerated aging test, cold test with soil and electrical conductivity. According to the results, it was found that the physiological potential of peanut seeds is inversely related to the drying temperature and the storage time, due to the fact that the germination and vigor are reduced as both factors increase. The air temperature of 40 °C may be recommended for the drying of peanut seeds, and even associated with the storage time of 150 days under uncontrolled conditions.


2013 ◽  
Vol 35 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Fábio Oliveira Diniz ◽  
Múcio Silva Reis ◽  
Luiz Antônio dos Santos Dias ◽  
Eduardo Fontes Araújo ◽  
Tuneo Sediyama ◽  
...  

With the aim of studying the relationship between the physiological quality of soybean seeds submitted to three harvest times and seedling emergence in the field, seeds of cultivars UFV-16, Splendor, Vencedora, Confiança, UFV-18, UFV-TN 105, Garantia and Celeste were harvested at stages R8, R8+15 and R8+30 days. The following tests were performed: accelerated aging, electrical conductivity, emergence in sand, emergence speed index and seedling emergence in the field. Germination and vigor were reduced in seeds harvested at 30 days after stage R8 in all the tests, except for the accelerated aging test, which showed a decline in vigor seeds harvested with a delay of 15 days. Seeds of cultivars UFV-16, Confiança and Garantia showed lower physiological quality in most tests, while the seeds of cultivar Celeste had higher physiological quality than the others. There was similarity between the test results of seed quality and seedling emergence in the field.


2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2891
Author(s):  
Fernanda Brito Cardoso ◽  
Carlos Henrique Queiroz Rego ◽  
Izabela Cristina de Oliveira ◽  
Alan Mario Zuffo ◽  
Ana Carina da Silva Cândido ◽  
...  

Chia (Salvia hispanica L.) is a plant native to central and western Mexico and northern Guatemala belonging to the Lamiaceae family, well known for its seeds containing antioxidants, dietary fibers, and ?-linolenic acid, which helps to prevent different diseases, such as diabetes, obesity, cancer and Alzheimer. In Brazil, there is not much information about the crop, mainly about the physiological quality of its seeds. Therefore, the objective was to establish vigor testing methods to evaluate the physiological quality of chia seeds. Five seed lots were used, in which germination tests, seedling emergence, emergence velocity index and variations in the electrical conductivity test were carried out. The volume of distilled water was 25 and 50 mL, the number of seeds was 50 and 100, the temperature was 20, 25 and 30 °C, the soaking period was 1, 2, 4, 6, 12, 18 and 24 hours and variations in there was accelerated aging test by the traditional method and saturated NaCl solution, being periods of aging as 24, 48, 72 and 96 hours and temperatures of 38, 41 and 44 °C. It is concluded that both the electrical conductivity test and the accelerated aging test are efficient in evaluating the physiological quality of chia seeds. The electrical conductivity test must be performed with 100 seeds in 50 mL of distilled water, for four hours soaking at 25 or 30 °C; and the accelerated aging test should be performed at 44 °C for 24 hours by the traditional method or 41 °C for 24 hours using saturated NaCl solution.


2021 ◽  
Vol 10 (36) ◽  
pp. 239-244
Author(s):  
Rosimar Maria Marques ◽  
Bruno Reis ◽  
Anielen C. Tormena Cavazin ◽  
Flávia Carolina Moreira ◽  
Monica Buchoski ◽  
...  

Rosimar Maria Marques1; Bruno Reis1; Anielen C. Tormena Cavazin1; Flávia Carolina Moreira1; Monica G. Buchoski1; Hingrid Ariane Silva1; Mayara A. Lois1; Carlos Moacir Bonato1* 1Universidade Estadual de Maringá – UEM 1* Orientador – [email protected] Introduction: The accelerated aging test evaluates the behavior of seeds exposed to stress conditions based on the fact that the rate of deterioration is considerably increased by exposure to high temperature and relative humidity [1]. The knowledge acquired in homeopathic science is experimental research on healthy living organisms that are undergoing experimentation using homeopathic medicines [2]. The experimental research on homeopathic plants has been performed in Europe, Mexico, India and Brazil, proving that the principle of homeopathy, as set out in the animal kingdom has been found in plants [3]. This experiment aimed to evaluate the effect of the homeopathic Arsenicum album in decimal (x) and centesimal (cH) scale on the physiological variables of the germination and growth of sorghum (Sorghum bicolor L.) subjected to accelerated aging. Materials and methods: The experiment was conducted at the Laboratory of Plant Physiology and Homeopathy, Universidade Estadual de Maringá - UEM. Arsenicum album 6x and 2CH matrices were acquired in homeopathic pharmacy. From matrices were prepared further dynamizations in decimal (x) and centesimal (C) scale, according to the Brazilian Homeopathic Pharmacopoeia [4]. In the accelerated aging test were used samples of 200 seeds per treatment distributed evenly forming a single layer on the surfaces of metal wire suspended inside the plastic box containing inside 40ml of Arsenicum album (9, 12, 18, or 30x 24 and 9, 12, 18, 24 or 30C). The control consisted of distilled water. The boxes were placed in BOD-chamber adjusted to 42°C and relative humidity 100% for 96 hours. After this period, led to the germination test, using four replicates of 50 seeds, totalizing 52 experimental units which were placed on germination paper previously soaked with distilled water in an amount equivalent to 2.5 times the paper weight[5]. The rolls were arranged randomly in the BOD-chamber at 25°C±2. The interpretation of the test was performed after seven days [6]. Germination percentage (% PG) was determined according to the Rules for Seed Analysis [5]. The length of primary roots (PRL) and hypocotyl (LH) were only performed in normal seedlings. The total length of the radicle (TLR) was calculated as the sum of the lengths of hypocotyl and primary root. The experimental design was completely randomized. We adopted the double-blind procedure, avoiding possible interference from the researcher. Data were subjected to analysis of variance and means compared by Scott-Knott test (P ≤ 0.05). Results: Arsenicum album 9x, 9CH and 12CH reduced the percentage of germination (%PG) (Figure 1A). The highest values in the length of hypocotyl (LH) ​​were observed in 12 and 24x dilutions, followed by a reduction in intermediate and higher inhibition at 30x and 9x. The 18x does not differ from control. All centesimal dilution presented effect on the variable analyzed. There was an increase in hypocotyl length in the 12, 18 and 24 dilutions, and reduction in 9 and 30 cH dilutions (Figure 1B). The primary root length (PRL) of sorghum was increased in dilutions 18 and 30x. However, the centesimal dilutions were not different from control (Figure 1C). There was an increase in the total length of the seedlings (TLS) in the dilutions 18, 24 and 30x and reduction in 9x when compared to control. As for centesimal dilutions, there was an effect only in 18cH. The other centesimal dilutions were not different from control (Figure 1D). Conclusions: In general, homeopathic preparations did not affect the germination percentage except for 9x, and 9cH 12cH dilution. Furthermore, the dilutions reduced the effects on premature aging verified by high values ​​of growth variables. These results suggest that high dilution Arsenicum album can be an alternative to minimize the deleterious effect to high temperature.


2021 ◽  
Vol 43 ◽  
Author(s):  
Rayssa Fernanda dos Santos ◽  
Henrique Fabrício Placido ◽  
Leonardo Lemes Bosche ◽  
Hugo Zeni Neto ◽  
Fernando Augusto Henning ◽  
...  

Abstract: This work evaluated the efficiency of accelerated aging test to determine the physiological potential in treated soybean seeds with fungicides and insecticides, in addition to analyzing the concordance between the results obtained by different accelerated aging methods with the actual results of the physiological quality in seeds after the storage. The study was divided into two experiments, both with entirely randomized delineation, with four replications. In the first experiment, seed germination was evaluated after imposing some treatments that were arranged in a 3 x 2 x 3 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); and accelerated aging (traditional; saturated NaCl solution; no aging). In the second experiment, seedling emergence was evaluated after applying some treatments that were arranged in a 3 x 2 x 2 factorial scheme: seed treatment (imidacloprid + thiodicarb; fipronil + thiophanate methyl + pyraclostrobin; no treatment); vigor levels (high; low); storage time (0 and 135 days). The accelerated aging methods were efficient to determine the physiological potential of the seeds, in which, the NaCl method presented better results. The physiological potential of soybean seeds decreased throughout storage and the application of chemicals in seed treatment impaired the germination and emergence percentages, regardless of the active ingredient used. The accelerated aging method with NaCl presents greater agreement with the actual storage results.


2012 ◽  
Vol 34 (2) ◽  
pp. 241-246 ◽  
Author(s):  
Denis Santiago da Costa ◽  
Cartiane Rubishirley Macêdo da Rocha ◽  
Ana Dionisia da Luz Coelho Novembre

The objective of this study was to investigate the possibility of using hydric restriction as a method for evaluating vigor of soybean seeds. The soybean seeds, cultivar BRS 245RR, represented by four different seed lots, were characterized by germination and vigor. For the treatment of hydric restriction and temperature, the combination of substrate water potential and temperature were the following: deionized water (0.0 MPa); polyethylene glycol (PEG 6000) aqueous solution (-0.1, -0.3 and -0.5 MPa); and four temperatures (20 ºC, 25 ºC, 30 ºC, and 35 ºC), respectively. A completely randomized experimental design was used, with four replications per treatment, and the ANOVA was performed individually for each combination of temperature and water potential of substrate. According to results obtained, the test of hydric restriction has the same efficiency of the accelerated aging test in estimating vigor of soybean seeds, cv. BRS 245RR, when water potentials of -0.1 MPa or -0.3 MPa at a temperature of 25 ºC, or -0.3 MPa at a temperature of 30 ºC are used.


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