Wound Healing Activity of a Traditionally Used Poly Herbal Product in a Burn Wound Model in Rats

Objective: The present study describes the anti-microbial acivity of Acacia arabica and Butea monosperma bark extract.Methods: For this purpose aqueous extract of bark were prepared by “Soxhlet extraction method”. The experimentally induced burn wound model in rats by “Excision method”.Results: As a result of this study it was found that the extract of bark generally revealed antimicrobial and wound healing activity.Conclusion: The result of the study suggest that the Acacia arabica and Butea monosperma bark of polyherbal gel effective in accelerating wound healing process.

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Determination of Wound Healing Activity of Shark Liver Oil using Burn Wound Model in Wistar Rats

Journal of Young Pharmacists ◽

10.5530/jyp.2021.13.24 ◽

2021 ◽

Vol 13 (2) ◽

pp. 113-117

Author(s):

Samiullah Allahbaksh Auti ◽

Rakesh Kumar Jat ◽

Supriya Kumar Das ◽

Shukla ST Shukla

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Burn Wound ◽

Shark Liver Oil ◽

Wound Model ◽

Wound Healing Activity

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Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽

10.3390/nano11030713 ◽

2021 ◽

Vol 11 (3) ◽

pp. 713

Author(s):

Nina Melnikova ◽

Alexander Knyazev ◽

Viktor Nikolskiy ◽

Peter Peretyagin ◽

Kseniia Belyaeva ◽

...

Keyword(s):

Wound Healing ◽

Zinc Oxide ◽

Bacterial Cellulose ◽

Zinc Oxide Nanoparticles ◽

Wound Dressings ◽

Oxide Nanoparticles ◽

Burn Wound ◽

Zno Nps ◽

Wound Model ◽

And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

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Wound Healing Activity of Avocado Peel Ointment against Second Degree Burn Wound

10.1109/inhence52833.2021.9537242 ◽

2021 ◽

Author(s):

Ma I Yang ◽

Ermi Girsang ◽

Ali Napiah Nasution ◽

Chrismis Novalinda Ginting

Keyword(s):

Wound Healing ◽

Burn Wound ◽

Degree Burn ◽

Wound Healing Activity ◽

Second Degree

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EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.23223 ◽

2018 ◽

Vol 11 (3) ◽

pp. 152

Author(s):

Sujith V ◽

Poornima G ◽

Balaji O ◽

Bairy Kl ◽

Praveen K ◽

...

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Chronic Wounds ◽

Statistical Significance ◽

Silver Sulfadiazine ◽

Histopathological Analysis ◽

Burn Wound ◽

Excision Wound ◽

Burn Wounds ◽

Wound Model

Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

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EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

Kongunadu Research Journal ◽

10.26524/krj17 ◽

2014 ◽

Vol 1 (1) ◽

pp. 73-77

Author(s):

Uma Maheswari P ◽

Shalimol A ◽

Arumugasamy A ◽

Udhaya Sankar M.R

Keyword(s):

Wound Healing ◽

Dry Weight ◽

Wound Contraction ◽

Albino Rats ◽

Standard Drug ◽

Excision Wound ◽

Wound Model ◽

Wistar Albino Rats ◽

Incision Wound ◽

Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

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Evaluation of wound healing activity of alpha mangostin ointment in rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4247 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 1080-1087

Author(s):

Sasikala Chinnappan ◽

Venkatalakshmi Ranganathan ◽

Jithendra Panneerselvam ◽

Barani Karikalan ◽

Thivashini vasanthan ◽

...

Keyword(s):

Wound Healing ◽

Wound Care ◽

Inflammatory Cells ◽

Treated Group ◽

Wound Model ◽

Wound Size ◽

Group I ◽

Significant Difference ◽

Histopathological Studies ◽

Wound Healing Activity

This study was aimed to evaluate the wound healing effects of alpha mangostin ointment using excision wound model. Twenty rats were divided into four groups of five rats each; group I was treated with ointment base (control), whereas group II, group III and group IV were treated with 10% (w/w) povidone-iodine (standard), alpha mangostin 1% (w/w), and alpha mangostin 2 % (w/w) respectively for wound healing evaluations. The size of the wound area was measured, and the reduction in the wound size was calculated, and the tissues examined histologically. The significant difference in the wound size reduction between the control and treated group (p<0.05) was observed in wound healing activity. Histopathological studies showed a lesser number of chronic inflammatory cells, lesser oedema and increased collagenisation in the test than control. This study showed that the alpha mangostin topical ointment could be a promising candidate for the betterment of wound care.

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EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i10.38674 ◽

2020 ◽

pp. 72-75

Author(s):

PRASANT KUMAR SABAT ◽

SWETA PRIYADARSINI PRADHAN ◽

RANDEEP PATRO

Keyword(s):

Wound Healing ◽

Tensile Strength ◽

Wound Contraction ◽

Control Group ◽

Albino Rats ◽

Green Electricity ◽

Wound Model ◽

Wound Protection ◽

Incision Wound ◽

Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

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Wound Healing Activity of Crassocephalum crepidioides (Benth.) S. Moore. Leaf Hydroethanolic Extract

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/2483187 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Nguyen Minh Can ◽

Dang Thi Phuong Thao

Keyword(s):

Wound Healing ◽

Fold Increase ◽

Inflammatory Cells ◽

Treated Group ◽

Pcr Analysis ◽

Wound Model ◽

Vehicle Treatment ◽

Hydroethanolic Extract ◽

Anti Inflammation ◽

Wound Healing Activity

Crassocephalum crepidioides (Benth.) S. Moore. has been used to treat small wounds by minority people in Lam Dong, Vietnam. However, there has been no scientific evidences about its wound healing activity. This study is aimed at evaluating the wound healing activity of Crassocephalum crepidioides hydroethanolic extract via its antioxidant and anti-inflammation activities and healing capability on a mouse excision wound model. Crassocephalum crepidioides hydroethanolic extract (CCLE) at a dose of 50 mg/kg/day reduced the wound closure time about 3.5 days, compared to vehicle treatment. The granulation tissue on day 7 after surgery from the treated group showed a 2.8-fold decrease in the density of inflammatory cells, 1.9-fold increase in the fibroblast density, and a higher number of blood vessels. Real-time PCR analysis indicated that the mRNA expression level of NF-κB1 and TNF-α mRNA in CCLE-treated wounds decreased by 4.6 and 3.3 times, respectively, while TGF-β1 and VEGF were found to increase by 3.3 and 2.4 times, respectively. Our experimental data provided proofs of Crassocephalum crepidioides leaf wound healing activity due to its antioxidant, anti-inflammation, fibroblast proliferation, wound contraction, and angiogenesis effects.

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