Supplementation of Pork Patties with Bovine Plasma Protein Hydrolysates Augments Antioxidant Properties and Improves Quality

Abstract Fermentable fiber may increase endogenous losses of P and AA, thereby reducing apparent nutrient digestibility. Acacia gum fiber with medium-to-high fermentability and low viscosity was used to investigate its effect on apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients, and standardized total tract digestibility (STTD) of P in grower pigs. A P-free basal diet (49% corn starch; 18% bovine plasma protein) was formulated to measure basal endogenous P losses (EPL). Three diets were formulated to include 2.5, 5.0, or 7.5% acacia gum fiber at the expense of corn starch in the P-free basal diet. Diets contained 16.1–17.4% CP and 0.31–0.33% total P (DM-basis). The 4 diets were fed to 8 ileal-cannulated barrows (initial BW, 54.6 kg) for four 9-d periods in a double 4 × 4 Latin square. Apparent hindgut fermentation (AHF) was ATTD minus AID. Increasing inclusion of acacia gum quadratically decreased (P < 0.01) AID of DM (∆ 11.1%), linearly decreased (P < 0.05) ATTD of DM (∆ 1.7%) CP (∆ 1.2%), and quadratically increased (P < 0.05) AHF of DM (∆ 9.4%). Basal EPL were 391 and 377 mg/kg DM intake (DMI) for ileum and total tract, respectively. Increasing inclusion of fiber linearly increased (P < 0.05) ileal EPL (∆ 184 mg/kg DMI), and tended to linearly increase (P < 0.10) EPL for total tract (∆ 243 mg/kg DMI). Dietary inclusion of acacia gum tended to linearly decrease (P < 0.10) AID of P, but did not affect (P > 0.10) ATTD, or STTD of P. In conclusion, increasing inclusion of fermentable, low viscous acacia gum fiber decreased diet digestibility of DM and ATTD of CP, but did not affect total tract P digestibility, indicating that increasing fermentable fiber did not increase specific endogenous losses of P in the total tract.

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Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-020-00531-5 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Tao Ran ◽

Long Jin ◽

Ranithri Abeynayake ◽

Atef Mohamed Saleem ◽

Xiumin Zhang ◽

...

Keyword(s):

Protein Synthesis ◽

Microbial Community ◽

Antioxidant Properties ◽

Value Added ◽

Gas Production ◽

Protein Hydrolysates ◽

Ruminal Fermentation ◽

Microbial Protein ◽

Microbial Protein Synthesis ◽

Spent Grain

Abstract Background Brewers’ spent grain (BSG) typically contains 20% – 29% crude protein (CP) with high concentrations of glutamine, proline and hydrophobic and non-polar amino acid, making it an ideal material for producing value-added products like bioactive peptides which have antioxidant properties. For this study, protein was extracted from BSG, hydrolyzed with 1% alcalase and flavourzyme, with the generated protein hydrolysates (AlcH and FlaH) showing antioxidant activities. This study evaluated the effects of AlcH and FlaH on gas production, ruminal fermentation characteristics, nutrient disappearance, microbial protein synthesis and microbial community using an artificial rumen system (RUSITEC) fed a high-grain diet. Results As compared to the control of grain only, supplementation of FlaH decreased (P < 0.01) disappearances of dry matter (DM), organic matter (OM), CP and starch, without affecting fibre disappearances; while AlcH had no effect on nutrient disappearance. Neither AlcH nor FlaH affected gas production or VFA profiles, however they increased (P < 0.01) NH3-N and decreased (P < 0.01) H2 production. Supplementation of FlaH decreased (P < 0.01) the percentage of CH4 in total gas and dissolved-CH4 (dCH4) in dissolved gas. Addition of monensin reduced (P < 0.01) disappearance of nutrients, improved fermentation efficiency and reduced CH4 and H2 emissions. Total microbial nitrogen production was decreased (P < 0.05) but the proportion of feed particle associated (FPA) bacteria was increased with FlaH and monensin supplementation. Numbers of OTUs and Shannon diversity indices of FPA microbial community were unaffected by AlcH and FlaH; whereas both indices were reduced (P < 0.05) by monensin. Taxonomic analysis revealed no effect of AlcH and FlaH on the relative abundance (RA) of bacteria at phylum level, whereas monensin reduced (P < 0.05) the RA of Firmicutes and Bacteroidetes and enhanced Proteobacteria. Supplementation of FlaH enhanced (P < 0.05) the RA of genus Prevotella, reduced Selenomonas, Shuttleworthia, Bifidobacterium and Dialister as compared to control; monensin reduced (P < 0.05) RA of genus Prevotella but enhaced Succinivibrio. Conclusions The supplementation of FlaH in high-grain diets may potentially protect CP and starch from ruminal degradation, without adversely affecting fibre degradation and VFA profiles. It also showed promising effects on reducing CH4 production by suppressing H2 production. Protein enzymatic hydrolysates from BSG using flavourzyme showed potential application to high value-added bio-products.

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Structural and antioxidant properties of cowpea protein hydrolysates

Food Bioscience ◽

10.1016/j.fbio.2021.101074 ◽

2021 ◽

pp. 101074

Author(s):

Andrea Gómez ◽

Carolina Gay ◽

Valeria Tironi ◽

Avanza María Victoria

Keyword(s):

Antioxidant Properties ◽

Protein Hydrolysates

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Bovine plasma protein as an alternative protein source in extruded aquafeeds for Piaractus mesopotamicus (pacú) at two fattening stages

Aquaculture Nutrition ◽

10.1111/anu.13015 ◽

2019 ◽

Vol 26 (2) ◽

pp. 536-544

Author(s):

Carla Bacchetta ◽

Andrea S. Rossi ◽

Raúl E. Cian ◽

David R. Hernández ◽

Sebastián Sánchez ◽

...

Keyword(s):

Plasma Protein ◽

Protein Source ◽

Piaractus Mesopotamicus ◽

Alternative Protein ◽

Bovine Plasma

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Antioxidant properties of papain mediated protein hydrolysates from fresh water carps (Catla catla, Labeo rohita and Cirrhinus mrigala) and its application on inhibition of lipid oxidation in oil sardine mince during ice storage

Journal of Food Science and Technology ◽

10.1007/s13197-021-05053-0 ◽

2021 ◽

Author(s):

Krishnamoorthy Elavarasan ◽

Bangalore Aswathnarayan Shamasundar

Keyword(s):

Lipid Oxidation ◽

Fresh Water ◽

Labeo Rohita ◽

Antioxidant Properties ◽

Protein Hydrolysates ◽

Catla Catla ◽

Ice Storage ◽

Cirrhinus Mrigala

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Anticoagulant properties of bovine plasma protein C following activation by thrombin

Biochemistry ◽

10.1021/bi00645a029 ◽

1977 ◽

Vol 16 (26) ◽

pp. 5824-5831 ◽

Cited By ~ 345

Author(s):

Walter Kisiel ◽

William M. Canfield ◽

Lowell H. Ericsson ◽

Earl W. Davie

Keyword(s):

Plasma Protein ◽

Protein C ◽

Bovine Plasma

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Enzymatic hydrolysis of chicken viscera proteins in the presence of an ionic liquid as a strategy to improve the antioxidant properties of the hydrolysates

Revista dos Trabalhos de Iniciação Científica da UNICAMP ◽

10.20396/revpibic2620181367 ◽

2019 ◽

Author(s):

Vitor Geniselli da Silva ◽

Ruann Janser Soares de Castro

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Enzymatic Hydrolysis ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Protein Hydrolysates ◽

Tetramethylammonium Bromide ◽

Chicken Viscera ◽

Hydrolysis Of

Aiming to explore the use of ionic liquids (ILs) not yet described in the literature, this work evaluated the hydrolysis of proteins from chicken viscera using the protease Alcalase modified and unmodified by the IL tetramethylammonium bromide. The protein hydrolysates produced in the presence of the IL presented values of antioxidant activities 40% higher than the hydrolysates obtained without IL. In addition, with the presence of the IL, it was possible to obtain protein hydrolysates from chicken viscera with similar antioxidant activities, compared to the protein hydrolysates produced without IL, using 1/3 of the amount of enzyme.

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