In vitro antimicrobial effect of silver nanoparticles on Lactococcus garvieae and Streptococcus iniae

Silver nanoparticles (Ag NPs) appeared as promising antimicrobial candidates to face the development of antibiotic resistance. Although reported as toxic towards mammalian cells, their combination with biomolecules have shown reduced toxicity, while maintaining the antimicrobial function. Herein, hyaluronic acid (HA) with low (40 kDa), medium (200 and 600 kDa) and high (2 MDa) molecular weight (Mw) was modified with adipic acid dihydrazide (ADH) and used as reducing and capping agents to synthesise antimicrobial hybrid Ag NPs. The Mw of the polymer played a crucial role in the morphology, size and antibacterial activity of the Ag NPs. The 600 and 200 kDa HA-ADH-Ag NPs were able to reduce the Escherichia coli and Staphylococcus aureus concentration by more than 3 logs, while the 40 kDa NPs reached ~2 logs reduction. The 2 MDa HA-ADH failed to form homogenous NPs with strong bactericidal activity. A mechanistic study of the interaction with a model bacterial membrane using Langmuir isotherms confirmed the greater interaction between bacteria and higher Mw polymers and the effect of the NP’s morphology. The nanocomposites low toxicity to human skin cells was demonstrated in vitro, showing more than 90% cell viability after incubation with the NPs.

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Synergistic Antifungal Activity of Green Synthesized Silver Nanoparticles and Epoxiconazole against Setosphaeria turcica

Journal of Nanomaterials ◽

10.1155/2020/9535432 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Weidong Huang ◽

Minhui Yan ◽

Haiming Duan ◽

Yaling Bi ◽

Xinxin Cheng ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antimicrobial Agents ◽

Integrated Control ◽

Antimicrobial Effect ◽

Colony Growth ◽

Setosphaeria Turcica ◽

Ligustrum Lucidum ◽

In Vitro Inoculation ◽

Average Size

It is urgent to develop highly efficient and eco-friendly antimicrobial agents for integrated control of phytopathogens. Silver nanoparticles (AgNPs) were synthesized by Ligustrum lucidum leaf extract. UV-vis spectrum showed that there was a strong absorbance at 438 nm. Transmission electron microscopy (TEM) images displayed that synthesized nanoparticles were near spherical with an average size of 13 nm. The antimicrobial effect of AgNPs was evaluated through methods of paper disk diffusion, colony growth, conidia germination, and in vitro inoculation. The 50% inhibition concentration (IC50) of AgNPs against Setosphaeria turcica was 170.20 μg/mL calculated by SPSS 13.0. In addition, it displayed a significant synergistic antifungal effect when AgNPs were combined with epoxiconazole at the ratios of 8 : 2 and 9 : 1. The results of this study provide a novel fungistat not only for comprehensive control of plant fungi but also for reducing chemical pesticides use and avoiding drug-resistant phytopathogen generation.

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Antimicrobial Efficacy of Silver Nanoparticles as Root Canal Irrigant’s: A Systematic Review

Journal of Clinical Medicine ◽

10.3390/jcm10061152 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1152

Author(s):

Shilpa Bhandi ◽

Deepak Mehta ◽

Mohammed Mashyakhy ◽

Hitesh Chohan ◽

Luca Testarelli ◽

...

Keyword(s):

Silver Nanoparticles ◽

Root Canal ◽

Antimicrobial Property ◽

Antimicrobial Effect ◽

Risk Of Bias ◽

Root Canals ◽

Root Canal Irrigants ◽

Endodontic Irrigation ◽

Time Restriction

Removal of microbes is imperative during endodontic therapy. Due to their antimicrobial property, silver nanoparticles have been used for endodontic irrigation of the root canals. The objective of the present study was to provide a qualitative analysis of the published literature assessing silver nanoparticles as root canal irrigants. A search of PubMed, SCOPUS, Web of Science, and Embase databases was done without any time restriction. Articles published in English were included. Data were extracted and the risk of bias was assessed. Of the 154 studies identified, after screening according to the inclusion criteria, five in vitro studies were included. The results indicate that silver nanoparticles have an anti-microbial effect to varying degrees depending on certain factors. Within the limitations of the present studies that have a moderate to low risk of bias, an antimicrobial effect of silver nanoparticles is observed. Silver nanoparticles have the potential to be used as endodontic irrigants, although their efficacy depends on particle size and the duration of contact which require further investigation.

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Bactericidal Capacity of Silver Nanoparticles Associated with Gantrez S-97 on Streptococcus Mutans

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.35.2.c61l421mj0655lgm ◽

2010 ◽

Vol 35 (2) ◽

pp. 183-185 ◽

Cited By ~ 2

Author(s):

Juan Francisco Hernández-Sierra ◽

Enid Karina Salas-López ◽

Fidel Martínez-Gutiérrez ◽

Facundo Ruíz ◽

Mauricio Pierdant-Pérez ◽

...

Keyword(s):

Public Health ◽

Experimental Study ◽

Silver Nanoparticles ◽

Antimicrobial Properties ◽

Public Health Problem ◽

Antimicrobial Effect ◽

Distilled Water ◽

Effective Agent ◽

Microdilution Method

Dental caries is a worldwide public health problem. S mutans plays an important role in the etiology of caries. There have been studies that showed the antimicrobial properties of silver nanoparticles are an effective agent to diminish S. mutans. The objective of this study was to evaluate the bactericidal and bacteriostatic effects of silver nanoparticles in addition to the Gantrez S-27 copolymer, on S mutans. Method:We performed an in vitro experimental study using the liquid microdilution method in order to find the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) with the subcultures obtained. The mixture was obtained by preparing 98 µg/mL of silver nanoparticles (10-3mol)with Gantrez S-27 2%, in distilled water. The readings were performed 24 hours after incubation and on 3 consecutive days. The results showed an average MIC of 6.12 µg /mL and MBC of 6.12 µg /mL.Conclusion: The addition of Gantrez 2% to silver nanoparticles does not alter its antimicrobial effect.

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Collagen-Carboxymethylcellulose Biocomposite Wound-Dressings with Antimicrobial Activity

Materials ◽

10.3390/ma14051153 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1153

Author(s):

Ionela Andreea Neacsu ◽

Sorina-Alexandra Leau ◽

Stefania Marin ◽

Alina Maria Holban ◽

Bogdan-Stefan Vasile ◽

...

Keyword(s):

Silver Nanoparticles ◽

Skin Diseases ◽

Chemical Characterization ◽

Antimicrobial Effect ◽

Skin Lesions ◽

Wound Dressings ◽

In Vitro Tests ◽

Absorption Bands ◽

Freeze Dried

Microbial infections associated with skin diseases are frequently investigated since they impact on the progress of pathology and healing. The present work proposes the development of freeze-dried, glutaraldehyde cross-linked, and non-cross-linked biocomposite dressings with a porous structure, which may assist the reepithelization process through the presence of collagen and carboxymethylcellulose, along with a therapeutic antimicrobial effect, due to silver nanoparticles (AgNPs) addition. Phisyco-chemical characterization revealed the porous morphology of the obtained freeze-dried composites, the presence of high crystalline silver nanoparticles with truncated triangular and polyhedral morphologies, as well as the characteristic absorption bands of collagen, silver, and carboxymethylcellulose. In vitro tests also assessed the stability, functionality, and the degradability rate of the obtained wound-dressings. Antimicrobial assay performed on Gram-negative (Escherichia coli), Gram-positive (Staphyloccocus aureus) bacteria, and yeast (Candida albicans) models demonstrated that composite wound dressings based on collagen, carboxymethylcellulose, and AgNPs are suitable for skin lesions because they prevent the risk of infection and have prospective wound healing capacity. Moreover, the cell toxicity studies proved that the obtained materials can be used in long time treatments, with no cytotoxic effects.

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THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2018.6.17 ◽

2018 ◽

Vol 17 (6) ◽

pp. 167-174 ◽

Cited By ~ 1

Author(s):

Małgorzata Schollenberger ◽

Tomasz M. Staniek ◽

Elżbieta Paduch-Cichal ◽

Beata Dasiewicz ◽

Agnieszka Gadomska-Gajadhur ◽

...

Keyword(s):

Essential Oils ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

Antimicrobial Effect ◽

Mentha Piperita ◽

Mentha Spicata ◽

Plant Essential Oils ◽

Plant Pathogenic Bacteria ◽

Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

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