scholarly journals The Regulatory Role of Different Exercises in Irisin, Heat Shock Protein 70 and Some Biochemical Parameters

2021 ◽  
Author(s):  
Taner Akbulut ◽  
Vedat Cinar ◽  
Suleyman Aydin ◽  
Meltem Yardim
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Biochemical Parameters ◽  
Exercise Program ◽  
Exercise Group ◽  
Human Organism ◽  
Control Group ◽  
Exercise Programs ◽  
Blood Samples

Background: It is known that exercise causes many changes in the human organism. Understanding the metabolisms underlying these changes is an important issue. The aim of the study was to determine the effects of different and regularly applied exercise programs on irisin, heat shock protein 70 and some biochemical parameters. Methods: 120 male university students participated in the study. Participants were divided into 4 equal groups as control (C), resistance exercise group (RE), high intensity interval (HIIT) and aerobic exercise group (AE). While the control group did not perform any exercise, the pre-determined exercise programs were applied to the other groups for 8 weeks and 3 days in a week. Blood samples were taken from all participants before and after the exercise program. Cholesterol, HDL and LDL cholesterol, triglyceride, creatine kinase (CK), lactate dehydrogenase (LDH), Irisin and Heat shock protein 70 (HSP70) levels were analyzed in blood samples. Results: It is determined that there are significant differences in pre-posttest values ​​of the AE group’s LDH, cholesterol, HDL, Triglyceride and HSP 70 levels, HIIT group’s CK, LDH, Cholesterol, HDL, Triglyceride, Irisin and HSP70 levels and RE group’s CK, LDH, Cholesterol, LDL, Triglyceride and irisin levels (p<0.05). Conlusions: It can be said that; exercise can correct abnormalities in lipid profile, changes in HSP70 levels may vary depending on muscle damage, the increase of irisin due to exercise.

scholarly journals Responses of Uric Acid, Glucose, Thyroid Hormones and Liver Enzymes to Aerobic and Combined Exercises in University Students

2020 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Taner Akbulut
Keyword(s):  
Uric Acid ◽  
Biochemical Parameters ◽  
Liver Enzymes ◽  
Exercise Program ◽  
Exercise Group ◽  
Control Group ◽  
Blood Samples ◽  
Glucose Levels ◽  
Combined Exercise ◽  
Post Test

Physiological effects of aerobic and anaerobic exercises are frequently investigated. However, it is considered that combined exercises may be more effective. The aim of this study was to investigate the effect of aerobic and combined exercise program on some biochemical parameters. The study group consisted of 45 male volunteers with sedentary life. The participants were divided into three groups as control (n: 15), aerobic exercise (n: 15) and combined exercise (n: 15). The control group did not perform any exercise. Aerobic and combined exercise groups participated in the exercise program 4 days a week for 10 weeks. Blood samples were taken from the participants twice. TSH, T3, T4, AST, ALT, Uric acid and glucose levels were determined in blood samples. SPSS software was used for the analysis of the data and the significance level was accepted as p &lt;0.05. As a result of analysis; no difference was observed in the control group (p&gt; 0.05). In the aerobic exercise group, T3, AST, Uric acid levels of post test were found to be different according to baseline, while in the combined exercise group, there were significant differences in T3, AST, Uric acid and glucose levels between pre and post test values (p &lt;0.05). As a result, it was determined that both aerobic and combined exercises had effects on biochemical parameters (thyroid hormones, liver enzymes, Uric acid and glucose). According to this study it can be said that combined exercises may be more effective than aerobic exercises.

scholarly journals PERUBAHAN EKSPRESI HEAT SHOCK PROTEIN 70 AKIBAT PAPARAN MEDAN ELEKTROMAGNETIK EXTREMELY LOW FREQUENCY PADA MAKROFAG PERITONEUM MENCIT YANG DIINFEKSI Toxoplasma gondii

Biomedika ◽  
2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Mochammad Arief Taufiqurochman
Keyword(s):  
Heat Shock ◽  
Toxoplasma Gondii ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Low Frequency ◽  
T Test ◽  
Control Group ◽  
Hsp 70 ◽  
Extremely Low Frequency ◽  
Promotor Region

Penelitian dilakukan untuk mengetahui efek paparan medan elektromagnetik ELF sebesar 100 μT 8 jam/ hari selama 2 dan 4 minggu terhadap ekspresi HSP 70 makrofag peritoneum mencit yang diinfeksi dengan Toxoplasma gondii. Jenis penelitian ini adalah Eksperimen Biomedik menggunakan rancangan randomized separate posttest control group designdengan hewan coba mencit strain Balb/c, melalui pengamatan ekspresi HSP 70 , terdiri dari 3 kelompok kontrol dan 4 kelompok perlakuan, tiap kelompok terdiri dari 4 hewan coba. Pengamatan jaringan menggunakan metode imunohistokimia indirek, hasilnya dianalisis menggunakan uji statistik Independent t-test antar kelompok setelah dilakukan uji homogenitas dan normalitas data penelitian ( α=0.05). Hasil penelitian menunjukkan bahwa akibat paparanME ELF dengan itensitas 100 μT selama 2 minggu belum mampu melemahkan atau memutus rantai DNA gen HSP 70 promotor region tetapi menimbulkan stres seluler yang berakibat teraktifasinya HSF 1 melalui konversi menjadi trimer yang akan meregulasi secara cepat sintesis HSP 70 . Paparan medan elektromegnetik ME ELF selama 4 minggu dapat melemahkan bahkan memutus rantai DNA hsp 70 promotor region, sehingga sintesis HSP akan terhambat secara signifikan (p<0.05). Terdapat peningkatan secara signifikan ekspresi HSP 70 makrofag peritoneum mencit yang terpapar ME ELF dengan itensitas 100 μT selama 2 minggu pada kelompok yang terinfeksi toxoplasma gondii dan terjadi penurunan secara signifikan ekspresi HSP 70 pada kelompok terpapar ME ELF selama4 minggu pada kelompok yang terinfeksi Toxoplasma gondi dibandingkan dengan konrol.Kata Kunci: Medan Electromagnetik ELF, HSP 70, Makrofag, Toxoplasma gondii.

scholarly journals Melatonin protects mouse oocyte from heat stress damage

2020 ◽  
Vol 6 (3) ◽  
pp. 13
Author(s):  
Haiyan Du ◽  
Shouhong Wang ◽  
Weiwei Huang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Spindle Assembly ◽  
Shock Group ◽  
Mouse Oocyte ◽  
Control Group ◽  
Mouse Oocytes ◽  
Early Apoptosis ◽  
Maturation Rate

Objective: To explore the potential effect of melatonin on the in-vitro maturation of mouse oocytes under heat shock condition.Methods: This study used a heat shock model of mouse oocyte maturation. The oocytes were randomly divided into three groups: control group, heat shock group and heat shock + melatonin group, in order to evaluate the effect of 1×10−9 mol/L melatonin on the quality of oocytes after heat shock.Results: In comparison with the control group, the maturation rate of mouse oocytes in heat shock group was significantly decreased [(33.00 ± 0.07)% vs. (85.00 ± 0.03)%, p < .01], with abnormal spindle assembly, and the early apoptosis rate was significantly increased [(59.7 ± 4.5)% vs. (22.0 ± 3.5)%, p < .01]. Compared with heat shock group, the maturation rate ofoocytes was significantly increased in heat shock + melatonin group [(70.00 ± 0.05)% vs. (33.00 ± 0.07)%, p < .01], meanwhile, the spindle abnormality rate and the early apoptosis rate were significantly decreased accordingly [(37.3 ± 6.1)% vs. (59.7 ± 4.5)%, p < .01]. The expression level of heat shock protein 70 was significantly up-regulated in heat shock + melatonin group in comparison with other two groups (p < .01).Conclusions: By regulating the over-expression of heat shock protein 70, melatonin can improve the declined maturation rate of oocytes and the increased rates of spindle assembly abnormality and early apoptosis caused by heat shock.

scholarly journals Myocardial Heat Shock Protein 70 Expression in Young and Old Rats After Identical Exercise Programs

2005 ◽  
Vol 60 (8) ◽  
pp. 963-969 ◽  
Author(s):  
J. W. Starnes ◽  
A. M. Choilawala ◽  
R. P. Taylor ◽  
M. J. Nelson ◽  
M. D. Delp
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Exercise Programs ◽  
Old Rats

scholarly journals THE EFFECT OF PSYCHOLOGICAL STRESS ON MPF INTRAFOLLICULAR

2019 ◽  
pp. 127-130
Author(s):  
REVI GH NOVIKA ◽  
BUDI SANTOSO ◽  
WIDJIATI WIDJIATI
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Psychological Stress ◽  
Heat Shock Protein 70 ◽  
Regulatory Protein ◽  
Control Group ◽  
Hsp70 Expression ◽  
Control Group Design ◽  
Cortisol Levels ◽  
Experimental Group

Objective: The aim of the study is to investigate the influence of cortisol and Heat Shock Protein 70 (HSP70) on the stressed mice to maturationpromoting factor (MPF) expression intrafollicular.Methods: Experimental laboratory with Randomized Post Test Only Control Group Design was carried out on intrafollicular mice. Divided into twogroups, experimental and control groups. The experimental group was given a 95 dB 4 hours/day noisy exposure for 5 days which was analogous topsychological stress in humans and the control group was not given noisy exposure. Furthermore, both groups were examined for cortisol levels toensure stress in mice. Heat shock protein 70 (HSP70) expression was examined as the main regulatory protein for stress response and Maturationpromoting factor (MPF) expression which is a mediator for oocyte maturation.Results: Psychological stress by 95 dB/4 hours/day noisy exposure for 5 days significantly increased serum cortisol levels in experimental group(p=0.000) and HSP70 expression (p=0.000). The effect of Increased cortisol levels and HSP70 expression significantly decrease in MPF expression(p=0.000).Conclusion: The study concluded that psychological stress could be seen by increasing cortisol and HSP70 expression affected to decreasing MPFexpression intrafollicular

The genetic diversity of heat shock protein 70 gene at promoter and 5' untranslated region in beef cattle

2021 ◽  
Vol 46 (2) ◽  
pp. 136-144
Author(s):  
I. Suhendro ◽  
J. Jakaria ◽  
R.R. Noor
Keyword(s):  
Genetic Diversity ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Beef Cattle ◽  
Heat Shock Protein 70 ◽  
Untranslated Region ◽  
Direct Sequencing ◽  
Single Nucleotide ◽  
Blood Samples ◽  
Sequencing Method

This study was aimed to identify genetic diversity in the promoter area and 5' UTR (untranslated region) HSP70 (heat shock protein 70) gene in several beef cattle in Indonesia. A total of 86 blood samples of Bali, Madura, PO (Peranakan Ongole), Limousine, and BB (Belgian Blue) cattle were used in this study. The extracted DNA of all blood samples was then analyzed using the direct sequencing method. The genotyping results showed the presence of five SNP (Single Nucleotide Polymorphism) in the promoter region, namely g.-393T>C, g.-343C>A, g.-202T>C and g.-69T>G and three SNPs at 5' UTR, i.e., g.19A>G, g.45C>T, and g.100_101ins30. The frequency of SNP alleles found in PO, Limousine, and BB cattle was in equilibrium, whereas in Bali and Madura cattle populations, the allele frequency was disequilibrium. In general, the level of diversity of observed alleles ranged from low to high (0.26-1.00), where SNP g.19A>G had the widest variety. It was successfully revealed in this analysis that the SNP g.-69T>G binds to both the NF-Y and CAAT box transcription factor. In addition, the 30 bp (base pair) insertions (g.100_101ins30) that were identified in Bali and Madura cattle have never been reported in previous research studies.

Inducing heat shock protein 70 expression provides a robust anti-thrombotic effect with minimal bleeding risk

2017 ◽  
Vol 117 (09) ◽  
pp. 1722-1729 ◽  
Author(s):  
Mikel Allende ◽  
Eva Molina ◽  
Ramón Lecumberri ◽  
Juan Sánchez-Arias ◽  
Ana Ugarte ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Ferric Chloride ◽  
Heat Shock Protein 70 ◽  
Bleeding Risk ◽  
Rank Test ◽  
Control Group ◽  
Hsp70 Expression ◽  
Bleeding Tendency ◽  
Dual Inhibitor

SummaryAntithrombotic medications target coagulation factors. Their use is associated with an increased bleeding risk. Safer drugs are needed. The heat shock protein 70 (Hsp70) exhibits antithrombotic properties that do not influence bleeding. By using murine models, we aimed to test the hypothesis that overexpressing Hsp70 with CM-695, a first in class dual inhibitor of HDAC6 and phosphodiesterase 9, protects against thrombosis while leaves bleeding tendency unaltered. CM-695 was used to induce Hsp70 overexpression. Hsp70 overexpressing mice were submitted to three thrombosis-triggering procedures. The ferric chloride carotid artery model was used to compare the antithrombotic role of CM-695 and rivaroxaban, a direct oral anticoagulant. The mouse tail transection model was used to compare the bleeding tendency upon CM-695 or rivaroxaban administration. Intraperitoneal (i. p.) 20 mg/kg CM-695 increased Hsp70 expression markedly in the murine aortic tissue. This treatment delayed thrombosis in the collagen/epinephrine [p=0.04 (Log-Rank test), n=10], Rose Bengal/laser [median vessel occlusion time (OT): 58.6 vs 39.0 minutes (min) in the control group (CG), p=0.008, n≥10] and ferric chloride (OT: 14.7 vs 9.2 min in the CG, p=0.032, n≥10) models. I.p. 80 mg/kg CM-695 (n≥9) and intravenous 3 mg/kg rivaroxaban (n≥8) significantly delayed thrombosis. CM-695 did not induce bleeding [median bleeding time (BT): 8.5 vs 7.5 min in the CG, n≥10]. However, BT was dramatically increased by rivaroxaban (30.0 vs 13.7 min in the CG, p=0.001, n=10). In conclusion, CM-695 is a new antithrombotic small molecule devoid of bleeding risk that may be envisioned as a useful clinical tool.

Differential effects of exercise and heat stress on liver HSP70 accumulation with aging

1996 ◽  
Vol 80 (2) ◽  
pp. 547-551 ◽  
Author(s):  
K. C. Kregel ◽  
P. L. Moseley
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Age Groups ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Passive Heating ◽  
Heating Rates ◽  
Colonic Temperature

Previous reports have suggested that the heat shock response to passive heating may be blunted by aging. However, during exertional heating, factors in addition to elevated temperature may amplify the degree of stress compared with hyperthermia alone. The purpose of this study was to compare the pattern of accumulation of the highly inducible 72-kDa heat shock protein (HSP72) in liver tissue of mature (12-mo-old) and senescent (24-mo-old) male Fischer 344 rats after either passive or exertional heat stress. A euthermic control group was exposed to an ambient temperature (Ta) of 25 degrees C for 4.5 h. A passive heating (heat) group was exposed to an Ta of 42 degrees C until colonic temperature (Tco) reached 41 degrees C. An exertional heating (exercise) group performed intermittent moderate-intensity treadmill exercise (similar absolute intensities for the two age groups) at an Ta of 32 degrees C until Tco reached 41 degrees C. Heating rates were similar in the heat and exercise groups (approximately 0.08 degrees C/min). Rats in both the heat and exercise groups were maintained at a Tco of 41 degrees C for an additional 30 min and subsequently returned to an Ta of 25 degrees C for 3 h. Liver HSP72 accumulation was increased in mature rats after both the heat (+192% vs. control) and exercise (+292%) protocols. In contrast, the senescent rats demonstrated no significant increase in inducible HSP70 with heating but a large increase with exercise (+232%; P < 0.01 compared with control and heat groups). These data suggest that the blunted heat shock protein response to heating observed with aging is not a result of the inability to produce inducible HSP72 because older rats had an robust response to exertional hyperthermia.

Cognitive Function and Heat Shock Protein 70 in Children With Temporal Lobe Epilepsy

2016 ◽  
Vol 32 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Azza M. Oraby ◽  
Ehab R. Abdol Raouf ◽  
Mostafa M. El-Saied ◽  
Maha K. Abou-Khadra ◽  
Suzette I. Helal ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Temporal Lobe Epilepsy ◽  
Temporal Lobe ◽  
Heat Shock Protein 70 ◽  
Short Term Memory ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Composite Scores

We conducted the present study to examine cognitive function and serum heat shock protein 70 levels among children with temporal lobe epilepsy. The Stanford-Binet Intelligence Test was carried out to examine cognitive function in 30 children with temporal lobe epilepsy and 30 controls. Serum heat shock protein 70 levels were determined with an enzyme-linked immunosorbent assay. The epilepsy group had significantly lower cognitive function testing scores and significantly higher serum heat shock protein 70 levels than the control group; there were significant negative correlations between serum heat shock protein 70 levels and short-term memory and composite scores. Children with uncontrolled seizures had significantly lower verbal reasoning scores and significantly higher serum heat shock protein 70 levels than children with controlled seizures. Children with temporal lobe epilepsy have cognitive dysfunction and elevated levels of serum heat shock protein 70, which may be considered a stress biomarker.

scholarly journals Association Of Nocturnal Intermittent Hypoxia With Heat Shock Protein 70 In Patients With Obstructive Sleep Apnea: A Pilot Study

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
Irina M. Madaeva ◽  
Nadezhda A. Kurashova ◽  
Natalya V. Semenova ◽  
Lubov I. Kolesnikova ◽  
Sergei I. Kolesnikov
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Intermittent Hypoxia ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Hsp70 Level ◽  
Obstructive Sleep

Introduction — Nocturnal intermittent hypoxia in obstructive sleep apnea (OSA) causes cellular stress and consequent change in inducible heat shock protein 70 (HSP70) level. Thus, the objective of this study was to determine the relationship among nocturnal hypoxia and the serum HSP70 level in patients with severe OSA. Material and Methods — The study involved 34 patients with a clinical diagnosis of moderate to severe OSA (24 men and 10 women). Patients without OSA (10 men and 5 women) were included as a control group. The groups were similar in age. The polysomnographic monitoring was carried by standart methodology. Blood sampling for determining the HSP70 level was carried out between 8:00 and 9:00 am after polysomnographic monitoring. Results — The results of this study demonstrated a high apnea/hypopnea index (AHI), which determined the OSA severity and decreased the blood oxygen saturation (SaO2) (p<0.05). Sleep fragmentation in OSA patients confirmed an increase in respiratory arousal index (ArI). The HSP70 level significantly increased in OSA patients compared with the control group. Correlation analysis showed a positive relationship between HSP70 and AHI (R=0.5) in patients with OSAS, as well as a negative relationship between HSP70 and SaO2 (R=-0.3). Conclusion — Our results demonstrated a high level of HSP70 in patients with severe OSA syndrome vs. those without it. In OSA patients, a direct correlation was found between the HSP70 level and AHI, as well as an inverse correlation between the AHI level and SaO2. These findings suggested an association between the level of inducible HSP70 and nocturnal hypoxia in OSA patients.

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