scholarly journals Composição da comunidade bacteriana aérea de Santarém, Pará: influência de aves

2020 ◽  
Vol 11 (7) ◽  
pp. 170-178
Author(s):  
Rídel Rodrigo Silva Fernandes ◽  
Suellen Caroline Barbosa Neves ◽  
Andresa Krislany Ferreira ◽  
Andreza Da Silva Peixoto ◽  
Graciene Do Socorro Taveira Fernandes
Keyword(s):  
Staphylococcus Spp ◽  
Lactobacillus Spp ◽  
Mycobacterium Spp

O ar livre é uma mistura que inclui poluentes biológicos, com potencial efeito sobre a saúde das pessoas e possível problema de saúde pública. Esta pesquisa teve por objetivo identificar a comunidade bacteriana do ar de áreas urbanas em Santarém-PA, e correlacionar a diversidade de microorganismos com a presença de aves. Entre agosto de 2017 a maio de 2018, pela técnica de sedimentação espontânea placas contendo PCA foram expostas em dez pontos em área urbana de Santarém. No Laboratório de Bacteriologia da Universidade Federal do Oeste do Pará, as placas foram incubadas a 35 °C por tempo máximo de 48 horas, nesse período foram realizadas contagens de bactérias seguido de isolamento e purificação das colônias. O processo de purificação se deu em meio TSA, seguida de teste morfotintorial pela coloração de Gram, e série bioquímica para identificação ao menor nível taxonômico possível. Entre os gêneros encontrados, destaque se deu para Staphylococcus spp., Mycobacterium spp. e Lactobacillus spp.. Os resultados, mostraram influência da presença das garças sobre a abundância e riqueza de bactérias, exceto no ponto controle, por ser uma área arborizada e não haver presença das aves, houve baixo crescimento bacteriano.

scholarly journals Efecto de la tintura de Propóleo vs Clorhexidina en el tratamiento de la Enfermedad Periodontal en caninos domésticos

2021 ◽  
pp. 81-85
Author(s):  
Diego Medina-Valarezo ◽  
Martha Camacho ◽  
Manuel García-Herreros ◽  
Andrés Ortega-Ojeda ◽  
Francisco Angulo-Cubillán
Keyword(s):  
Staphylococcus Spp ◽  
Lactobacillus Spp

En el presente estudio se analizó el efecto de la tintura de propóleo (TP) vs clorhexidina (C) en el tratamiento de la enfermedad periodontal en la especie canina (EPC). Para ello se llevó a cabo la aplicación terapéutica en 30 pacientes con diferentes grados de enfermedad periodontal (leve, media y grave). Se tomaron muestras de la cavidad bucal para el cultivo posterior e identificación de los microorganismos patógenos implicados en el proceso patológico. Se identificaron 4 géneros de patógenos: Staphylococcus spp., Corynebacterium spp., Lactobacillus spp. y Enterobacterias. El porcentaje de casos que respondieron al tratamiento fue 80 y 3,4 % para TP y C, respectivamente. C no demostró tener ninguna acción eficiente en el tratamiento de EPC. Sin embargo, TP mostró ser el tratamiento de elección, independientemente del patógeno, asociación de éstos o el grado de progresión de la EPC.

scholarly journals Antibacterial and Antioxidant Activity of Metabolites From Bioconverted Docosahexaenoic Acid Using Gut Bacteria

2021 ◽  
Author(s):  
Visali Kannan ◽  
Rubavathi Anandan ◽  
Dinesh Kumar Sudalaimani ◽  
Shantkriti Sriniva ◽  
Murugan Athiappan
Keyword(s):  
Escherichia Coli ◽  
Docosahexaenoic Acid ◽  
Salmonella Enteritidis ◽  
Bacterial Isolates ◽  
Enterococcus Spp ◽  
Clinical Pathogens ◽  
Staphylococcus Spp ◽  
Antibacterial And Antioxidant Activity ◽  
Lactobacillus Spp

Abstract Docosahexaenoic acid (DHA) is an essential fatty acid necessary for brain development in both infants and adults. However, the role of gut microbiome and their metabolites produced from DHA remain unclear. In present study, the bacterial isolates Lactobacillus spp. Clostridium spp. Escherichia coli; Staphylococcus spp. Enterococcus spp. were used to convert the metabolites from DHA with SM medium supplemented with 200mg of DHA as substrate. The metabolites were extracted after 24 hours of incubation at 37°C and analyzed by GC/MS. The antimicrobial activity of these metabolites confirmed their effectiveness against clinical pathogens such as Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis and Staphylococcus aureus.

scholarly journals Agentes etiológicos de mastitis bovina en municipios con importante producción lechera del departamento de Boyacá

2015 ◽  
Vol 2 (2) ◽  
pp. 162
Author(s):  
Jenny Carolina Hernández Barrera ◽  
Maritza Angarita Merchán ◽  
Diego Alejandro Benavides Sánchez ◽  
Carlos Fernando Prada Quiroga
Keyword(s):  
Staphylococcus Aureus ◽  
Palabras Clave ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Lactobacillus Spp

Introducción: La mastitis es considerada como una infección que afecta la ubre de la vaca en diversos grados de gravedad, ocasionada por numerosos microorganismos. Trae como consecuencia una reducción en la producción de leche y la alteración de sus características fisicoquímicas. La calidad higiénica de la leche tiene una importancia fundamental en el consumo humano y, por tanto, debe ser un producto inocuo e idóneo. Objetivo: Este estudio pretende dar a conocer los principales agentes etiológicos de la mastitis bovina y sus patrones de sensibilidad a los antibióticos, dando una visión global de su situación actual en los municipios de importante producción lechera del departamento de Boyacá. Métodos: Mediante un estudio cuantitativo, descriptivo, observacional y transversal, se procesaron 214 muestras de leche prevenientes de pezones afectados con algún grado de mastitis bovina. Para el diagnóstico microbiológico se emplearon muestras de leche a las que se les aplicaron protocolos habituales de identificación, siguiendo los perfiles bioquímicos. Los patrones de sensibilidad a antibióticos se establecieron mediante la técnica de Kirby-Bauer. Resultados: Se encontró una prevalencia de 31 % (n=214) de bacterias que ocasionan mastitis contagiosa, siendo Staphylococcus aureus el agente etiológico más importante en un 26 %. Se presentó una alta sensibilidad a los antibióticos betalactámicos evidente en el 20,6 % de los aislamientos, correspondiente a bacterias Gram positivas presentando resistencia a la penicilina, especialmente en especies de Staphylococcus spp. Conclusiones: En este trabajo se aislaron e identificaron algunos agentes etiológicos causantes de mastitis bovina de origen contagioso, origen ambiental y agentes oportunistas e infrecuentes (Bacillus spp. y Lactobacillus spp.). En estos aislamientos se encontró una alta sensibilidad a antibióticos, principalmente a los betalactámicos. Palabras clave: leche, mastitis bovina, microbiología, antibiograma. 

scholarly journals ПОКАЗНИКИ АНТИЛІЗОЦИМНОЇ ТА АНТИКОМПЛЕМЕНТАРНОЇ АКТИВНОСТІ МІКРООРГАНІЗМІВ ДИСТАЛЬНОГО ВІДДІЛУ КИШЕЧНИКА ПОРОСЯТ ЗА ЗМІШАНОЇ НЕМАТОДОЗНО-ПРОТОЗООЗНОЇ ІНВАЗІЇ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 248-251
Author(s):  
R.A. Peleno
Keyword(s):  
Intestinal Parasites ◽  
Anticomplementary Activity ◽  
Candida Spp ◽  
Distal Intestine ◽  
E Coli ◽  
Vital Functions ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Lactobacillus Spp

The article deals with the results of search of the influence of intestinal parasites on the indicators of antilysozyme and anticomplementary activity of microorganism of distal department of intestine. It was established, that the products of intestinal parasites of pigs reduce anti lysozyme activity of such obligate anaerobes as Bifidobacterium spp. to 42.1% (р < 0.01), Prevotella spp. to 14.3%, Clostridium spр. to 66.7% (р < 0,001). With MAFAnM to 38.5% (р < 0.05) in Lactobacillus spp. and to 50.0% (р < 0.01) in Streptococcus spp. Higher compared with the control, antilysozyme activity was in Bacteroides spp. (р < 0.05), Eubacterium spp. (р < 0,001), Propionіbacterium spp. (р < 0.01), Peptostreptococcus spp. (р < 0.05), Fusobacterium spp. (р < 0.01), E. coli lact. «+» hem «-» (р < 0.05), Klebsiella spp. (р < 0.001), Citrobacter spp. (р < 0.01), Enterococcus spp. (р < 0.05), Staphylococcus spp. (р<0.001), Candida spp. (р<0.001) abstracted from the distal intestine of pigs affected with mixed nematodosis - protoozic invasion. With the action of products of vital functions of ascarices, eymeria and balantides, the anticomplementary activity significantly increases in Prevotella spp. (р < 0.01), Clostridium spр. (р < 0.001), Eubacterium spp. (р < 0.001), Propionіbacterium spp. (р < 0.001), Peptostreptococcus spp. (р < 0,001), Fusobacterium spp. (р < 0.001), Lactobacillus spp. (р < 0.05), E. coli lact. «+» hem «-» (р < 0.01), E. coli lact. «-» hem «+» (р < 0.001), Klebsiella spp. (р < 0.05), Enterobacter spp. (р < 0.001), Enterococcus spp. (р < 0.001), Streptococcus spp. (р < 0.001) and only in Bacteroides spp. it was significantly lower (р < 0.001), compared with the control.

scholarly journals Microbiota indígena do meato acústico externo de gatos hígidos

1998 ◽  
Vol 28 (3) ◽  
pp. 441-445 ◽  
Author(s):  
Ricardo Coutinho do Amaral ◽  
José Fernando Ibañez ◽  
Elsa Masae Mamizuka ◽  
Walderez Gambale ◽  
Claudete Rodrigues de Paula ◽  
...  
Keyword(s):  
Pseudomonas Sp ◽  
Penicillium Sp ◽  
Trichoderma Sp ◽  
Alternaria Sp ◽  
Acinetobacter Sp ◽  
Aureobasidium Sp ◽  
Aspergillus Sp ◽  
Staphylococcus Spp ◽  
Otodectes Cynotis ◽  
Lactobacillus Spp

Cinqüenta felinos hígidos, adultos - vinte e seis machos e vinte e quatro fêmeas - sem definição racial, com distintas idades, reunidos em dois grupos (GRUPO 1- animais domiciliados e GRUPO 2 - animais que rendados) de vinte e cinco animais cada. Após anamnese e prévia sedação, os gatos foram submetidos a exame físico e à otoscopia, comprovando-se a ausência de lesões meatais e timpânicas, com cureta de Buck e wragatoas estéreis, foi colhido material meatal, que foi então submetido a exames bacteriológico e micológico. No Grupo 1, foi evidenciado presença de Otodectes cynotis (4% das amostras), em 72% dos casos de bolores e leveduras (Cladosporium sp 66,6%, Malassezia sp 40%, Penicillium sp 33%, Aspergillus sp 33,3%, Rhodotorula sp 20,0%, Mycelia sp 13,3% e Alternaria sp, Aureobasidium sp, Ryzopus sp, Trichosporon sp, todos com 6,6%) e, finalmente, em 64% da amostragem, bactérias dos gêneros Staphylococcus spp (81,2%), Pseudomonas sp (12,5%), Klebsiella sp (12,5%), Acinetobacter sp, Bacilos difteróides, Enterobacter sp, Lactobacillus spp (todos com 6,2%). No Grupo 2, o Otodectes sp foi identificado em 36% das amostras, em 96% daquelas isolaram-se fungos dos gêneros: Malassezia sp - 54,1%, Aspergillus e Penicillium sp, ambos com 33,3%, Microsporum sp - 29,1%, Cladosporium sp - 16,6%, Trichoderma sp - 12,5%, Alternaria e Phoma sp, ambos com 8,3% e Epicoccum sp, Neurospora sp. Mycelia sp, Rhodotorula sp, todos com 4,1% e, por fim, em 20 das 25 amostras (80%) isolaram-se pelo menos uma cepa bacteriana (Staphylococcus spp 75%, Klebsiella sp 20,8%, Bacilos difteróides 12,5%, Pseudomonas sp, 8,3%) e Acinetobacter sp, Enterobacter sp e Escherichia sp, todos com 4,1% cada um em cultivo monoespecífico ou em associação.

scholarly journals Cervical Canal Microbiota Features in Second Trimester of Pregnancy in Insuficiencia Istmicocervical with Clinical Signs of Vulvovaginitis Depending on Vaginal Discharge рH

Doctor Ru ◽  
2021 ◽  
Vol 20 (6) ◽  
pp. 12-19
Author(s):  
N.V. Spiridonova ◽  
◽  
M.A. Kaganova ◽  
O.O. Devyatova ◽  
A.A. Bezrukova ◽  
...  
Keyword(s):  
Vaginal Discharge ◽  
Clinical Signs ◽  
Cervical Canal ◽  
Study Group ◽  
Second Trimester ◽  
Study Objective ◽  
Bacteriological Test ◽  
Study Results ◽  
Staphylococcus Spp ◽  
Lactobacillus Spp

Study Objective: To study the microbiological composition of cervical canal in insuficiencia istmicocervical (IIC) in patients with vulvovaginitis depending on vaginal discharge рH. Study Design: perspective study. Materials and Methods. The study included 67 patients of reproductive age with clinical signs of vulvovaginitis and IIC. Where vaginal discharge рН was > 4.5, patients were prescribed empirical Metrogil Plus; these women comprised the study group (n = 40). Where vaginal discharge рН was ≤ 4.5, patients were prescribed empirical Tergynan; these women were controls (n = 27). Baseline anthropometric, social, clinical and anamnestic data, pregnancy characteristics and parity were recorded; then women underwent examination, and samples were collected for analysis (bacterioscopy, vaginal discharge рН measurement, bacteriological test). On day 9–11 of hospitalisation, patients had another speculum examination; clinical symptoms dynamics, pH and bacterioscopic test results were evaluated. Satisfaction with therapy, compliance and therapy-related arverse events were recorded. Study Results. Various Staphylococcus spp. were the most prevalent cause of the conditions in the study group (n = 17; 42.5%). In controls this value was 12 (44.4%). Staphylococcus spp. were mostly opportunistic saprophytic flora and usually were not pathogens, except for Staphylococcus haemolyticus, which was found in 11 (27.5%) patients in the study group and in 3 (11.1%) controls. Lactobacillus spp. Was diagnosed more often in controls than in the study group: 12 (44.4%) vs. 9 (22.5%) (р = 0.05). The control group was diagnosed only with Lactobacillus crispatus, Lactobacillus jensenii; they also prevailed in the study group, together with Lactobacillus gasseri, Lactobacillus paracasei. At рН > 4.5, cervical canal culture was characterised with a variety of opportunistic pathogens and normal flora. After the therapy, patients in both groups did not complain of vaginal discharges. Physical examination demonstrated normal vaginal wall colour and moderate discharges. Vaginal discharge pH was significantly lower in the study group and was normal in controls. Conclusion. The majority of patients with vulvovaginitis and IIC in the second trimester of pregnancy had lower prevalence as well as lower absolute and relative amount of Lactobacillus spp. in their cervical canal discharge. At the same time, increase in vaginal рН is associated with changes in the quality ratio of lactic acid bacilli. Keywords: cervical canal microbiota, insuficiencia istmicocervical, vaginal discharge рH, vulvovaginitis

scholarly journals Lipolitical, lisocycle, and hemolitical activity of microflors of pigs intestine, invased in the association of askaris, eyeria and balantidia

2018 ◽  
Vol 20 (83) ◽  
pp. 376-380
Author(s):  
R.A. Pelenio ◽  
V.V. Stybel ◽  
V.O. Ushkalov
Keyword(s):  
Hemolytic Activity ◽  
Lysozyme Activity ◽  
Candida Spp ◽  
Distal Intestine ◽  
E Coli ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Lactobacillus Spp ◽  
Clostridium Spp

The changes in lipolytic, lysozyme and hemolytic activity of the intestinal microflora of piglets invaded with the association of ascaris, imyrium and balantidium have been studied. It has been established that among microorganisms isolated from the distal intestine of piglets, lipase is synthesized by Bacteroides spp., Prevotella spp., Clostridium sp. and Peptostreptococcus spp., E. coli lact. «-» hem +, Enterobacter spp., Streptococcus spp., Staphylococcus spp. and Candida spp. In pigs affected by ascaris, emerya, and balantidium, the number of strains producing lipase was higher compared to healthy ones, namely: Bacteroides spp. – by 20.2%, Prevotella spp. – by 3.7%, Clostridium spp. – 29.2% and Peptostreptococcus spp. – by 14.9%, Staphylococcus spp. – by 67.7%, Streptococcus spp. – by 19.1%, E. coli lact. «-» heme «+» – by 17.3%, Candida spp. – 16.5% and Enterobacter spp. – by 4.8%. Lysozyme activity was detected in Bifidobacterium spp., Lactobacillus spp. and Staphylococcus spp. Significant differences in the penetrance of lysozyme activity of microorganisms in healthy and affected by ascarids, imedium, and balantidias of piglets were observed in Lactobacillus spp. and Staphylococcus spp. (P < 0.001). At the same time, in piglets, compared with healthy animals, the number of Lactobacillus spp. Strains that showed lysozyme activity decreased, while Bifidobacterium spp. and Staphylococcus spp. – grew. Bifidobacterium spp., Eubacterium spp., Lactobacillus spp., E. coli lact. «+» Hem «-», Enterobacter spp. and Citrobacter spp. there is no ability to produce hemolysins. Among lactosonegative gastric sticks, all strains isolated from both healthy and sick pigs showed hemolytic activity. The associative influence of ascaris, imerium and balantidii, showed an increase in the penetrance of hemolytic activity in Bacteroides spp. – by 45.8%, Prevotella spp. – by 26.8%, Clostridium spp. – 42.1%, Propionibacterium spp. – 41.5%, Peptostreptococcus spp. – 29.1% and Fusobacterium spp. – by 16.4%, Klebsiella spp. – by 33.4%, Enterococcus spp. – by 69.6%, Streptococcus spp. – by 66.0%, Staphylococcus spp. – by 64.5% and Candida spp. – by 11.5%.

Changes in the Natural Microflora During Incubation of Fresh and Processed Human Milks1

1982 ◽  
Vol 45 (9) ◽  
pp. 792-794
Author(s):  
N. SHEWAKRAMANI ◽  
M. O. HANNA ◽  
E. S. ALFORD ◽  
C. VANDERZANT ◽  
C. W. DILL ◽  
...  
Keyword(s):  
Post Partum ◽  
Raw Milk ◽  
Coagulase Negative Staphylococcus ◽  
Coryneform Bacteria ◽  
Fresh Milk ◽  
Natural Microflora ◽  
Plate Counts ◽  
Low Levels ◽  
Staphylococcus Spp ◽  
Lactobacillus Spp

Seven pooled human milks, each from at least 6 different breast-feeding mothers (4 weeks post-partum) were studied for microbial population and flora changes during storage at 37°C. Coagulase-negative Staphylococcus spp. constituted a considerable part (39–100%) of the microflora in 6 of 7 pools of fresh milk, with coryneform bacteria, Lactobacillus, Micrococcus and Streptococcus spp. isolated less frequently. Storage of raw milk for 8 h caused a shift in flora favoring the Lactobacillus spp. After 24 h, coagulase-negative Staphylococcus were dominant in 4 of 7 pools, coryneform bacteria in 2 of 7, and Lactobacillus and Micrococcus spp. each in 1 of 7 pools. Low levels of bacteria were detected in 2 of 7 pasteurized pools, consisting of Staphylococcus spp. or yeasts. Freezing of the milk before storage at 37°C had little effect on the aerobic plate counts.

Peculiarities of the mouth and colum in the youth of annous age with arterial hypertension and metabolic disorders

2020 ◽  
Vol 65 (11) ◽  
pp. 712-716
Author(s):  
V. M. Chervinets ◽  
Yu. V. Chervinets ◽  
Elina Sergeevna Kravchuk
Keyword(s):  
Bacillus Subtilis ◽  
Arterial Hypertension ◽  
Metabolic Disorders ◽  
Oral Fluid ◽  
Quantitative Composition ◽  
Frequency Of Occurrence ◽  
Medical University ◽  
Staphylococcus Spp ◽  
Lactobacillus Spp ◽  
Intestinal Microbiocenosis

The purpose of the work is to determine the species, quantitative composition and frequency of occurrence of microorganisms in the oral fluid and large intestine in healthy young men and having arterial hypertension, and metabolic disorders. A survey was conducted of 51 young students studying at Tver State Medical University. It was found that more than 70% of students with AH (arterial hypertension) and MBN (metabolic disorders) showed pronounced dysbiotic disorders of the intestinal microbiota of II and III degree with a decrease in the number of Lactobacillus spp., Bifidobacterium spp., Peptococcus spp. and an increase in the number and frequency of occurrence of Bacillus subtilis, Clostridium spp., S. aureus, Klebsiella pneumoniae. The intestinal microbiocenosis of students with AH and MS is characterized by a decrease in the number and frequency of occurrence of Lactobacillus spp. However, with MS, the prevalence (frequency) and quantity increase Staphylococcus spp., Streptococcus spp., Peptostreptococcus spp., Proteus vulgaris, Bacillus subtilis, and in hypertension - Bacillus subtilis, Neisseria, Actinomyces, Peptostreptococcus spp.

Phospholipid analogue profiles of Peptostreptococcus, Micromonas, and Finegoldia species analysed by fast atom bombardment mass spectrometry

2001 ◽  
Vol 47 (1) ◽  
pp. 96-101 ◽  
Author(s):  
C E Radcliffe ◽  
D B Drucker ◽  
V Boote ◽  
G Fletcher-Williams ◽  
M A Claydon
Keyword(s):  
Mass Spectrometry ◽  
Fast Atom Bombardment ◽  
Soft Tissues ◽  
Anaerobic Growth ◽  
Negative Ion ◽  
Mucous Membranes ◽  
Negative Ion Mode ◽  
Staphylococcus Spp ◽  
Chloroform Methanol ◽  
Lactobacillus Spp

Species of Peptostreptococcus cause a variety of infections, primarily abscesses of soft tissues, joints, and mucous membranes. The aim of this study was to compare the phospholipid analogue profiles of Peptostreptococcus species, represented by P. anaerobius, P. asaccharolyticus, P. indolicus, P. lacrimalis, and P. prevotii; Micromonas micros (P. micros) and Finegoldia magna (P. magnus). After anaerobic growth on blood-FAA, lipids extracted by chloroform-methanol (2:1 v/v) were purified, then analysed by fast atom bombardment mass spectrometry (FAB-MS) in negative ion mode. The major peaks with mass to charge (m/z) 719, 721, and 749, corresponded to phosphatidylglycerol analogues, namely PG (32:1), PG (32:0), and PG (34:0), which have been found previously in Lactobacillus spp., Clostridium difficile, and Staphylococcus spp. Other major peaks observed, with m/z 619, 647, 665, 675, 677, 687, 691, 693, 701, 703, 707, 733, and 746 have also been reported in one or more of these three species. However, other major peaks found here in Peptostreptococcus, Micromonas, and Finegoldia have not been described elsewhere; these are 501, 514, 515, 618, 659, 673, 676, 688, 690, 692, 694, 700, 706, 715, 718, 722, and 750. We conclude that Peptostreptococcus, Micromonas, and Finegoldia isolates are chemically unique.Key words: Peptostreptococcus, Micromonas, Finegoldia, phospholipids, FAB-MS.

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