scholarly journals Some aspects of in vitro wheat biotechnology

2019 ◽  
Vol 25 ◽  
pp. 316-320
Author(s):  
L. E. Sergeeva ◽  
L. I. Bronnikova
Keyword(s):  
Winter Wheat ◽  
Cell Cultures ◽  
Cellular Level ◽  
In Vitro System ◽  
Distinctive Features ◽  
Cultural Conditions ◽  
Special Adaptation ◽  
Primary Explants ◽  
Plant Level

Aim. Drastic climate changes lead to decrease of the appropriate agricultural plants and stimulate the elaboration of new biotechnologies. The preferences of in vitro system are used for providing the acceleration of the plant selection. The cultivating in vitro is a procedure combined common approaches and special adaptation to plant species. This ideology is essential for all cereals and for wheat in particular. There are several aspects of this ideology: the optimization of cultural conditions; the obtaining wheat cultures and studying distinctive features of their proliferation; the detection parameters of viability, realized on the entire plant level; the comparison of those reactions with cells characteristics. Methods. The standard manipulations of primary explants dissection and several protocols of callus induction and raise are used. Results. Cell cultures of new wheat genotypes were obtained. Those forms were selected in the Institute of Plant Physiology and Genetics NAS of Ukraine. The peculiar features of wheat cell cultures were revealed and investigated. Conclusions. Cell cultures obtained from new genotypes of winter wheat demonstrated common reactions with young plants. Parallel investigations of some biochemical parameters realized on cellular level in cell cultures and plant cells is a possible way to acceleration the genotypes with better characteristics selection. Keywords: winter wheat, in vitro system, cell culture.

scholarly journals Uptake and utilization of mRNA by myogenic cells in culture.

1980 ◽  
Vol 87 (1) ◽  
pp. 65-71 ◽  
Author(s):  
B Mroczkowski ◽  
H P Dym ◽  
E J Siegel ◽  
S M Heywood
Keyword(s):  
Cell Cultures ◽  
Rna Synthesis ◽  
Tissue Specificity ◽  
Kinase Activity ◽  
Actinomycin D ◽  
Creatine Kinase Activity ◽  
Dependent Manner ◽  
In Vitro System ◽  
Myoblast Cell

Primary chick myoblast cultures demonstrate the ability to take up exogenously supplied polyadenylated RNA and express the encoded information in a specific manner. This expression is shown to exhibit tissue specificity. Analysis of creatine kinase activity monitored at various times of incubation in the presence of either polyadenylated or nonpolyadenylated RNA indicates that only the poly(A)+ mRNA is capable of being actively translated. Radioactively labled poly(A)+ mRNA is taken up by the cell cultures in a time-dependent manner and subsequently shown to be associated with polysomes. This association with polysomes does not occur in the presence of puromycin and is unaffected by actinomycin D. Thus, nonspecific interaction with polysomes and induction of new RNA synthesis are ruled out and the association of the exogenously supplied poly(A)+ mRNA with polysomes is indicative of its translation in the recipient cells. When heterologous mRNA (globin) is supplied to the myoblasts, it is also taken up and properly translated. In addition, exogenously supplied myosin heavy chain mRNA is found associated with polysomes consisting of 4-10 ribosomes in myoblast cell cultures while in myotubes it is associated with very large polysomes, thus reflecting the different translational efficiencies that this message exhibits at two very different stages of myogenesis. The results indicate that muscle cell cultures can serve as an in vitro system to study translational controls and their roles in development.

Role of the gut proteinases from mosquito larvae in the mechanism of action and the specificity of the Bacillus sphaericus toxin

1990 ◽  
Vol 36 (11) ◽  
pp. 804-807 ◽  
Author(s):  
Luc Nicolas ◽  
Anne Lecroisey ◽  
Jean-François Charles
Keyword(s):  
Cell Cultures ◽  
Spodoptera Littoralis ◽  
Mosquito Species ◽  
Bacillus Sphaericus ◽  
Cellular Level ◽  
Mosquito Cell ◽  
Mosquito Larvae

Gut proteinases from larvae of mosquito species both susceptible and not susceptible to Bacillus sphaericus converted the 43-kDa toxin to a 40-kDa polypeptide exhibiting enhanced cytotoxicity to mosquito cell cultures. The toxin was also activated by gut proteinases from the nonsusceptible Lepidoptera Spodoptera littoralis in vitro and in vivo. Therefore, the specificity of Bacillus sphaericus toxin does not seem to be determined by gut proteinase action. However, susceptibility of mosquito cell cultures did not reflect the specificity of the toxin, which must now be investigated at the cellular level in the larvae. Key words: mosquitoes, Bacillus sphaericus, bacterial toxins, proteinases, specificity.

Pharmacological responsiveness of dissociated native and cultured eccrine secretory coil cells

1990 ◽  
Vol 258 (6) ◽  
pp. R1355-R1362
Author(s):  
H. Yokozeki ◽  
K. Saga ◽  
F. Sato ◽  
K. Sato
Keyword(s):  
Cultured Cells ◽  
Cellular Level ◽  
Dependent Manner ◽  
In Vitro System ◽  
Ductal Cells ◽  
Dissociated Cells ◽  
14Co2 Production ◽  
Secretory Coil ◽  
Dose Dependent

Methacholine (MCh)- and isoproterenol (Iso)-stimulated 14CO2 production was compared between freshly dissociated rhesus sweat secretory coil cells (mainly clear cells) and cultured cells (grown on a collagen-coated plastic plate) derived from native cells. 14CO2 production was enhanced by MCh and by Iso in native coil cells (but not in ductal cells) in a pharmacologically specific and dose-dependent manner. 14CO2 production in subcultured coil cells (19-45 days in culture) was only one-third to one-fifth that of native cells. MCh-stimulated 14CO2 production was inhibited by ouabain and furosemide in both native and cultured coil cells. A decrease in 14CO2 production, of about one-half, was already evident in primary cells cultured for less than 1 wk. The decreased pharmacological responsiveness of the cultured coil cells was seen, although the cultured cells showed the typical epithelioid appearance, abundant mitochondria, the occasional presence of intercellular lacunae resembling intercellular canaliculi, and the persistence of immunoreactive keratin. We conclude that 1) a primary culture of sweat gland cells can be initiated from dissociated cells; 2) cultured sweat secretory coil cells qualitatively, but not quantitatively, retain the pharmacological responsiveness and transport activity of the native cells as determined by 14CO2 production; 3) collagenase-dissociated cells represent an excellent in vitro system for the study of glandular function at the cellular level; and 4) the decrease in pharmacological responsiveness is not simply due to trypsin treatment during harvesting of cultured cells, because that of organ-cultured, intact, secretory coils also declines with time of culture.

scholarly journals Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yi-Hsuan Lin ◽  
Hsing-Yu Chen ◽  
Jung-Chun Chiu ◽  
Kun-Jei Chen ◽  
Hung-Yao Ho ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Inflammatory Cytokine ◽  
Skin Diseases ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Cellular Level ◽  
Immunomodulatory Effect ◽  
Markers Of Inflammation ◽  
Schizonepeta Tenuifolia

Schizonepeta tenuifolia (ST) Briq. is a traditional herbal medicine commonly used to treat allergic skin diseases, where the inflammation process is closely related to symptom severity. This study aimed to explore the immunomodulatory effect of ST by using immunoglobulin E- (IgE-) stimulated RBL-2H3 cell cultures, a common cell line for studying mast cell degranulation and inflammatory cytokine release in vitro. After stimulating the RBL-2H3 cells with IgE, ST at concentrations of 10, 50, or 100 μg/mL was added to the cell cultures. Cell viability, inflammatory cytokines (IL-6, IL-13, IL-4, TNF-α, and IFN-γ), anti-inflammatory cytokine IL-10, and degranulation ability were examined 48 and 72 hours after administration of ST. The markers of inflammation and allergic reaction, IFN-γ, TNF-α, IL-4, and IL-6, were suppressed, especially after treatment with 100 μg/mL ST. However, the anti-inflammation marker IL-10 was also suppressed by ST. Trend analysis showed that a higher ST concentration was associated with lower IFN-γ and TNF-α levels. Moreover, degranulation of RBL-2H3 cells was assessed by measuring the release of β-hexosaminidase, which was suppressed by ST at 10 μg/mL. This study showed an immunomodulatory effect of ST at the cellular level and suggests the role of ST in treating allergic diseases.

An in vitro system for studying the effects of ozone on mammalian cell cultures and viruses

1982 ◽  
Vol 27 (2) ◽  
pp. 466-475 ◽  
Author(s):  
David C. Bolton ◽  
Brian K. Tarkington ◽  
Yuan Chung Zee ◽  
John W. Osebold
Keyword(s):  
Mammalian Cell ◽  
Cell Cultures ◽  
In Vitro System ◽  
Mammalian Cell Cultures

scholarly journals In vitro Behaviour and Effects on Cells Induced by Functionalized NanoGold and NanoSilver Particles I. Preparation and Stability Studies of Au- and Ag-conjugates with Thiol - containing Molecules

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Carmen SOCACIU ◽  
Andreea STANILA ◽  
Monica TRIF ◽  
Simion ASTILEAN
Keyword(s):  
Cell Cultures ◽  
Extreme Values ◽  
Cellular Level ◽  
Stability Studies ◽  
Mean Values ◽  
Color Changes ◽  
Antioxidant Agents ◽  
Nanosilver Particles ◽  
And Shifts

Our studies aim the preparation of functionalized Au and Ag colloids invitro, the dynamics of conjugation and stability in functionalized forms (by conjugation with cysteine, glutathione, insulin, albumin) (in this first part of article) as well and to study their action on cells (second part). Such investigations can prove that natural ways of Au and Ag functionalization, by formation of stable conjugates, with S-containing biomolecules can be active at cellular level and can act synergistically as protective, antioxidant agents. We determined dimensions of colloid Au (AuC) vs colloid Ag (AgC) and found out mean values between 0.863m to 0.86 m.Using a calibration curve for AuC in the range 350-800 nm, we found a good correlation concentration-absorption units and the best dilutions to be used in conjugation experiments (1:2-1:5 ). The kinetic of AuC conjugation with four different molecules (cystein, BSA, insulin and glutathion), was specifically expressed by shifts of around 3 nm (for cystein, insulin and albumin) suggesting that AuC cannot make directly stable conjugates with these molecules. The same kinetic, tested for AuC-glutathion, showed spectacular change of color from red to blue and shifts of the absorbtion of conjugated forms, up to 16 nm (from 600 to 616 nm). Contrasting to Au, AgC in the presence of glutathion did not show modifications of absorptions, nor color changes. Therefore, we consider that Ag cannot conjugate glutathion and it is not useful to test it on cell cultures in vitro. Using different pHs (from 3 to 8) we observed an increase of conjugation capacity of AuC with glutathione, up to values 7-7.5. At extreme values (pH 3 and 8) the conjugation is not possible.

The Pharmaceutical Aerosol Deposition Device on Cell Cultures (PADDOCC) In Vitro System: Design and Experimental Protocol

2010 ◽  
Vol 38 (4) ◽  
pp. 285-295 ◽  
Author(s):  
Stephanie Hein ◽  
Michael Bur ◽  
Tobias Kolb ◽  
Bernhard Muellinger ◽  
Ulrich F. Schaefer ◽  
...  
Keyword(s):  
System Design ◽  
Cell Cultures ◽  
Aerosol Deposition ◽  
Experimental Protocol ◽  
In Vitro System ◽  
Pharmaceutical Aerosol

Reactions of Human Endothelial Cell Cultures Exposed to Adrenalin Concentrations

1975 ◽  
Author(s):  
Payling H. Wright ◽  
M. Evans
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Cultures ◽  
Vascular Endothelium ◽  
Human Endothelial Cell ◽  
Cultural Conditions ◽  
Endothelial Cell Cultures

Cultures of vascular endothelium obtained from fresh human umbilical veins and grown in vitro in fortified 199 medium for several days have been subjected to differing concentrations of adrenalin for various times. Their reactions to the drug, as seen microscopically, were recorded photographically. The viability of endothelial cells under these cultural conditions gives a measure of the maximal exposure to adrenalin which they are able not only to survive, but also to multiply. Their capacity to mitose was studied autor adiographically.The significance of the findings will be discussed with reference to atherogenesis and particularly the possible link with infection and in “stress”.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

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