scholarly journals Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yi-Hsuan Lin ◽  
Hsing-Yu Chen ◽  
Jung-Chun Chiu ◽  
Kun-Jei Chen ◽  
Hung-Yao Ho ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Inflammatory Cytokine ◽  
Skin Diseases ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Cellular Level ◽  
Immunomodulatory Effect ◽  
Markers Of Inflammation ◽  
Schizonepeta Tenuifolia

Schizonepeta tenuifolia (ST) Briq. is a traditional herbal medicine commonly used to treat allergic skin diseases, where the inflammation process is closely related to symptom severity. This study aimed to explore the immunomodulatory effect of ST by using immunoglobulin E- (IgE-) stimulated RBL-2H3 cell cultures, a common cell line for studying mast cell degranulation and inflammatory cytokine release in vitro. After stimulating the RBL-2H3 cells with IgE, ST at concentrations of 10, 50, or 100 μg/mL was added to the cell cultures. Cell viability, inflammatory cytokines (IL-6, IL-13, IL-4, TNF-α, and IFN-γ), anti-inflammatory cytokine IL-10, and degranulation ability were examined 48 and 72 hours after administration of ST. The markers of inflammation and allergic reaction, IFN-γ, TNF-α, IL-4, and IL-6, were suppressed, especially after treatment with 100 μg/mL ST. However, the anti-inflammation marker IL-10 was also suppressed by ST. Trend analysis showed that a higher ST concentration was associated with lower IFN-γ and TNF-α levels. Moreover, degranulation of RBL-2H3 cells was assessed by measuring the release of β-hexosaminidase, which was suppressed by ST at 10 μg/mL. This study showed an immunomodulatory effect of ST at the cellular level and suggests the role of ST in treating allergic diseases.

scholarly journals IgE Antibodies against Cancer: Efficacy and Safety

Antibodies ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 55
Author(s):  
Jitesh Chauhan ◽  
Alex J. McCraw ◽  
Mano Nakamura ◽  
Gabriel Osborn ◽  
Heng Sheng Sow ◽  
...  
Keyword(s):  
Perceived Risk ◽  
Immunoglobulin E ◽  
Ex Vivo ◽  
Risk Mitigation ◽  
Antitumour Activity ◽  
Allergic Diseases ◽  
Type I ◽  
Ige Antibodies

Immunoglobulin E (IgE) antibodies are well known for their role in allergic diseases and for contributions to antiparasitic immune responses. Properties of this antibody class that mediate powerful effector functions may be redirected for the treatment of solid tumours. This has led to the rise of a new class of therapeutic antibodies to complement the armamentarium of approved tumour targeting antibodies, which to date are all IgG class. The perceived risk of type I hypersensitivity reactions following administration of IgE has necessitated particular consideration in the development of these therapeutic agents. Here, we bring together the properties of IgE antibodies pivotal to the hypothesis for superior antitumour activity compared to IgG, observations of in vitro and in vivo efficacy and mechanisms of action, and a focus on the safety considerations for this novel class of therapeutic agent. These include in vitro studies of potential hypersensitivity, selection of and observations from appropriate in vivo animal models and possible implications of the high degree of glycosylation of IgE. We also discuss the use of ex vivo predictive and monitoring clinical tools, as well as the risk mitigation steps employed in, and the preliminary outcomes from, the first-in-human clinical trial of a candidate anticancer IgE therapeutic.

Role of the gut proteinases from mosquito larvae in the mechanism of action and the specificity of the Bacillus sphaericus toxin

1990 ◽  
Vol 36 (11) ◽  
pp. 804-807 ◽  
Author(s):  
Luc Nicolas ◽  
Anne Lecroisey ◽  
Jean-François Charles
Keyword(s):  
Cell Cultures ◽  
Spodoptera Littoralis ◽  
Mosquito Species ◽  
Bacillus Sphaericus ◽  
Cellular Level ◽  
Mosquito Cell ◽  
Mosquito Larvae

Gut proteinases from larvae of mosquito species both susceptible and not susceptible to Bacillus sphaericus converted the 43-kDa toxin to a 40-kDa polypeptide exhibiting enhanced cytotoxicity to mosquito cell cultures. The toxin was also activated by gut proteinases from the nonsusceptible Lepidoptera Spodoptera littoralis in vitro and in vivo. Therefore, the specificity of Bacillus sphaericus toxin does not seem to be determined by gut proteinase action. However, susceptibility of mosquito cell cultures did not reflect the specificity of the toxin, which must now be investigated at the cellular level in the larvae. Key words: mosquitoes, Bacillus sphaericus, bacterial toxins, proteinases, specificity.

Diagnostic and analytical performance of a screening panel for allergy

2005 ◽  
Vol 43 (9) ◽  
Author(s):  
Ileana Herzum ◽  
Nicole Blümer ◽  
Werner Kersten ◽  
Harald Renz
Keyword(s):  
Immunoglobulin E ◽  
Wide Spectrum ◽  
Screen Test ◽  
Allergic Diseases ◽  
Analytical Performance ◽  
Screening Tests ◽  
Food Allergies ◽  
Early Assessment ◽  
Prick Test

AbstractWorldwide, allergic diseases are increasing in prevalence and incidence. Early assessment of the immunoglobulin E (IgE) sensitisation status has a major impact on clinical outcome and selection of therapeutic options. Recently, a number of new IgE-detecting test systems have entered the market, including screening tests allowing identification of a wide spectrum of sensitising allergens. We evaluated the analytical and diagnostic performance of the newly developed Allergy Screen test panel for atopy (Mediwiss Analytic, Moers, Germany). The evaluation was performed for four major respiratory and four major nutritional allergens in 142 patients with clinical suspicion of respiratory and/or food allergies. For all allergens, the test showed acceptable concordance to the skin-prick test and the in vitro IgE CAP system (Pharmacia, Freiburg, Germany). The analytical performance was acceptable, with CVs between 2 and 8% in the positive range and good dilution linearity (R=0.9735). Imprecision in the low IgE concentration range dramatically improved by lowering the cut-off value to 0.2IU/mL IgE. In conclusion, the Allergy Screen panel yields reliable results in the detection of allergic sensitisation to common allergens.

Efficacy of hyposensitization in feline allergic diseases based upon results of in vitro testing for allergen-specific immunoglobulin E

1997 ◽  
Vol 33 (3) ◽  
pp. 282-288 ◽  
Author(s):  
RE Halliwell
Keyword(s):  
Allergic Disease ◽  
Immunoglobulin E ◽  
Clinical Signs ◽  
Allergic Diseases ◽  
Dietary Change ◽  
In Vitro Tests ◽  
Similar Response ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

A survey was undertaken to evaluate the responses to hyposensitization in cats for which a diagnosis of allergic disease was made by assessment of clinical signs and evaluation of allergen-specific immunoglobulin E (IgE) using the radioallergosorbent test (RAST). Eighty-one cases were available for analysis. In 39 cases, a dietary change resulted in some subjective improvement, which usually was minimal; however, some cases had significant improvement. In 75.3% of cases, an improvement of at least 50% was noted and ascribed to a combination of immunotherapy and dietary change, when implemented. The response of the 42 cases in which immunotherapy alone was used or where there was no response to dietary change was broadly similar. Response of the specific dermatological conditions ranged from 93.6% in the case of linear granuloma to 60% in cases where self-induced hair loss was evident. Response also was good in cases of presumed allergic asthma (86.1% improvement) and suspected allergic otitis externa (62.6% improvement). In nine patients, the hyposensitization was discontinued due to lack of response. The results suggest that confirmation of a suspected diagnosis of allergic disease in cats by means of in vitro tests and subsequent therapy with hyposensitization should be a major consideration in feline practice.

scholarly journals The Ethanol Extract of Holotrichia diomphalia Larvae, Containing Fatty acids and Amino acids, Exerts Anti-Asthmatic Effects through Inhibition of the GATA-3/Th2 Signaling Pathway in Asthmatic Mice

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 852 ◽  
Author(s):  
Jung-Hee Hong ◽  
Seung-Hyung Kim ◽  
Young-Cheol Lee
Keyword(s):  
Allergic Asthma ◽  
Immunoglobulin E ◽  
Ethanol Extract ◽  
Allergic Diseases ◽  
Eosinophil Infiltration ◽  
Th2 Cytokines ◽  
Cell Hyperplasia ◽  
Vitro Assay ◽  
Anti Inflammatory

Holotrichia diomphalia larvae (HD), a natural product from an insect resource, possesses many pharmacological properties, including anticoagulant, antitumor, anti-inflammatory, and analgesic activity. The major bioactive ingredients include oleic acid, palmitic acid, palmitoleic acid, linoleic acid, proline, and glutamic acid. Although HD is associated with immunoregulatory activities in allergic diseases, the therapeutic mechanisms of the action of HD in allergic diseases have not been investigated. The aim of this study was to evaluate the anti-asthmatic potential of HD in an ovalbumin (OVA)-induced mouse model of allergic asthma. Moreover, the anti-inflammatory potential of HD was examined to identify a plausible mechanism of action of HD in vitro. HD strongly reduced goblet cell hyperplasia, eosinophil infiltration, and reactive oxygen species (ROS), which reduced airway hyperresponsiveness (AHR), inflammation, and the expression of Th2 cytokines (IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF). The expression of IL-5, IL-4, eotaxin-2, lysyl oxidase-like 2 (loxl2), and GATA-binding protein 3 (GATA-3) was attenuated in the lungs. In an in vitro assay, HD exerted immunomodulatory effects through the suppression of Th2 cytokines (IL-5, IL-13), IL-17, and tumor necrosis factor (TNF)-α production through downregulation of GATA-3 expression in EL-4 T cells. These findings suggest that the anti-asthmatic activity of HD may occur through the suppression of Th2 cytokines and total Immunoglobulin E (IgE) production by inhibition of the GATA-3 transcription pathway. Our results suggest that HD may be a potential alternative therapy, or a novel therapeutic traditional medicine, for the treatment of allergic asthma.

scholarly journals Wheat Bran Extract Regulates Mast Cell-Mediated Allergic Responses In Vitro and In Vivo

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3997 ◽  
Author(s):  
Jae Yeon Lee ◽  
Eun-Kyung Ahn ◽  
Ju-Hyoung Park ◽  
Joa Sub Oh
Keyword(s):  
Mast Cell ◽  
Wheat Bran ◽  
Molecular Mechanisms ◽  
Immunoglobulin E ◽  
Water Extract ◽  
Allergic Diseases ◽  
Mitogen Activated Protein Kinase ◽  
Food Ingredients

In the present study the effects and molecular mechanisms of wheat bran (WB), the hard outer layer of the wheat kernel used in food ingredients, on mast cell-mediated allergic responses in vitro and in vivo were investigated. The water extract of WB inhibited degranulation and expression of allergic and inflammatory mediators such as tumor necrosis factor-α, cyclooxygenase-2 and inducible nitric oxide synthase in antigen-stimulated RBL-2H3 cells. These anti-allergic activities of WB were mediated by the inactivation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, which play important roles in degranulation and expression of various allergic and inflammatory molecules. In agreement with its in vitro effects, WB inhibited immunoglobulin E (IgE)/antigen-induced and compound 48/80-induced anaphylactic reactions in vivo. Taken together, these findings suggest the pharmacological potential of WB in the regulation of allergic diseases, including allergic rhinitis, atopic dermatitis, asthma and anaphylaxis.

scholarly journals In vitro Behaviour and Effects on Cells Induced by Functionalized NanoGold and NanoSilver Particles I. Preparation and Stability Studies of Au- and Ag-conjugates with Thiol - containing Molecules

2010 ◽  
Vol 67 (2) ◽  
Author(s):  
Carmen SOCACIU ◽  
Andreea STANILA ◽  
Monica TRIF ◽  
Simion ASTILEAN
Keyword(s):  
Cell Cultures ◽  
Extreme Values ◽  
Cellular Level ◽  
Stability Studies ◽  
Mean Values ◽  
Color Changes ◽  
Antioxidant Agents ◽  
Nanosilver Particles ◽  
And Shifts

Our studies aim the preparation of functionalized Au and Ag colloids invitro, the dynamics of conjugation and stability in functionalized forms (by conjugation with cysteine, glutathione, insulin, albumin) (in this first part of article) as well and to study their action on cells (second part). Such investigations can prove that natural ways of Au and Ag functionalization, by formation of stable conjugates, with S-containing biomolecules can be active at cellular level and can act synergistically as protective, antioxidant agents. We determined dimensions of colloid Au (AuC) vs colloid Ag (AgC) and found out mean values between 0.863m to 0.86 m.Using a calibration curve for AuC in the range 350-800 nm, we found a good correlation concentration-absorption units and the best dilutions to be used in conjugation experiments (1:2-1:5 ). The kinetic of AuC conjugation with four different molecules (cystein, BSA, insulin and glutathion), was specifically expressed by shifts of around 3 nm (for cystein, insulin and albumin) suggesting that AuC cannot make directly stable conjugates with these molecules. The same kinetic, tested for AuC-glutathion, showed spectacular change of color from red to blue and shifts of the absorbtion of conjugated forms, up to 16 nm (from 600 to 616 nm). Contrasting to Au, AgC in the presence of glutathion did not show modifications of absorptions, nor color changes. Therefore, we consider that Ag cannot conjugate glutathion and it is not useful to test it on cell cultures in vitro. Using different pHs (from 3 to 8) we observed an increase of conjugation capacity of AuC with glutathione, up to values 7-7.5. At extreme values (pH 3 and 8) the conjugation is not possible.

scholarly journals Peripheral blood basophils are the main source for early interleukin-4 secretion upon in vitro stimulation with Culicoides allergen in allergic horses

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0252243
Author(s):  
Fahad Raza ◽  
Susanna Babasyan ◽  
Elisabeth M. Larson ◽  
Heather S. Freer ◽  
Christiane L. Schnabel ◽  
...  
Keyword(s):  
Immune Responses ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Interleukin 4 ◽  
Th2 Cells ◽  
Peripheral Blood Mononuclear ◽  
New Findings

Interleukin-4 (IL-4) is a key cytokine secreted by type 2 T helper (Th2) cells that orchestrates immune responses during allergic reactions. Human and mouse studies additionally suggest that basophils have a unique role in the regulation of allergic diseases by providing initial IL-4 to drive T cell development towards the Th2 phenotype. Equine Culicoides hypersensitivity (CH) is a seasonal immunoglobulin E (IgE)-mediated allergic dermatitis in horses in response to salivary allergens from Culicoides (Cul) midges. Here, we analyzed IL-4 production in peripheral blood mononuclear cells (PBMC) of CH affected (n = 8) and healthy horses (n = 8) living together in an environment with natural Cul exposure. During Cul exposure when allergic horses had clinical allergy, IL-4 secretion from PBMC after stimulation with Cul extract was similar between healthy and CH affected horses. In contrast, allergic horses had higher IL-4 secretion from PBMC than healthy horses during months without allergen exposure. In addition, allergic horses had increased percentages of IL-4+ cells after Cul stimulation compared to healthy horses, while both groups had similar percentages of IL-4+ cells following IgE crosslinking. The IL-4+ cells were subsequently characterized using different cell surface markers as basophils, while very few allergen-specific CD4+ cells were detected in PBMC after Cul extract stimulation. Similarly, IgE crosslinking by anti-IgE triggered basophils to produce IL-4 in all horses. PMA/ionomycin consistently induced high percentages of IL-4+ Th2 cells in both groups confirming that T cells of all horses studied were capable of IL-4 production. In conclusion, peripheral blood basophils produced high amounts of IL-4 in allergic horses after stimulation with Cul allergens, and allergic horses also maintained higher basophil percentages throughout the year than healthy horses. These new findings suggest that peripheral blood basophils may play a yet underestimated role in innate IL-4 production upon allergen activation in horses with CH. Basophil-derived IL-4 might be a crucial early signal for immune induction, modulating of immune responses towards Th2 immunity and IgE production.

scholarly journals Some aspects of in vitro wheat biotechnology

2019 ◽  
Vol 25 ◽  
pp. 316-320
Author(s):  
L. E. Sergeeva ◽  
L. I. Bronnikova
Keyword(s):  
Winter Wheat ◽  
Cell Cultures ◽  
Cellular Level ◽  
In Vitro System ◽  
Distinctive Features ◽  
Cultural Conditions ◽  
Special Adaptation ◽  
Primary Explants ◽  
Plant Level

Aim. Drastic climate changes lead to decrease of the appropriate agricultural plants and stimulate the elaboration of new biotechnologies. The preferences of in vitro system are used for providing the acceleration of the plant selection. The cultivating in vitro is a procedure combined common approaches and special adaptation to plant species. This ideology is essential for all cereals and for wheat in particular. There are several aspects of this ideology: the optimization of cultural conditions; the obtaining wheat cultures and studying distinctive features of their proliferation; the detection parameters of viability, realized on the entire plant level; the comparison of those reactions with cells characteristics. Methods. The standard manipulations of primary explants dissection and several protocols of callus induction and raise are used. Results. Cell cultures of new wheat genotypes were obtained. Those forms were selected in the Institute of Plant Physiology and Genetics NAS of Ukraine. The peculiar features of wheat cell cultures were revealed and investigated. Conclusions. Cell cultures obtained from new genotypes of winter wheat demonstrated common reactions with young plants. Parallel investigations of some biochemical parameters realized on cellular level in cell cultures and plant cells is a possible way to acceleration the genotypes with better characteristics selection. Keywords: winter wheat, in vitro system, cell culture.

scholarly journals Effectiveness of Sublingual Immunotherapy in the Treatment of HDM-Induced Nasobronchial Allergies: A 3-Year Randomized Case-Control Study From Kashmir

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahid M. Baba ◽  
Roohi Rasool ◽  
Ayaz Gull ◽  
Taha A. Qureshi ◽  
Afaq H. Beigh ◽  
...  
Keyword(s):  
Clinical Improvement ◽  
Sublingual Immunotherapy ◽  
Immunoglobulin E ◽  
Allergic Diseases ◽  
Immunological Tolerance ◽  
Controlled Study ◽  
Prick Test ◽  
Disease Modifying

Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders that induces immunological tolerance through administration of specific allergens. Studies on AIT for subcutaneous route are in abundance; however, the efficacy of AIT in tablet form through sublingual route has not been well elucidated. The present prospective, parallel-group, controlled study sought to compare the efficacy of sublingual immunotherapy (SLIT) tablets with pharmacotherapy (PT) in 332 house dust mite (HDM)-specific allergic asthma and/or rhinitis patients over a period of 3 years. Patients were followed up for a 6-month run-in period and then randomly stratified as those who would receive SLIT, SLIT in addition to PT (SLIT+PT), and PT alone. AIT was administered in the form of sublingual tablets. Symptom and medication scores were measured every 3 months. In vitro evaluation of serum total and HDM specific immunoglobulin E (HDM sIgE) levels was carried out every 3 months, whereas in vivo skin prick test was performed annually for 3 years. Our study demonstrated sustained clinical improvement, reduction in inhaled corticosteroid (ICS) dose and duration as well as prevention from development of neosensitization to other aero allergens in HDM-allergic asthmatics and/or rhinitis patients treated with 3 years SLIT. Despite a remarkable clinical improvement with AIT, we observed that SLIT did not significantly change the skin reactivity to HDM at 3 years and there was no significant change in the ratio of serum total and HDM sIgE. Given the immune and disease modifying effects of AIT in allergic diseases, the present study supports the notion of its sublingual mode being an effective long-term immunomodulator in HDM-sensitized nasobronchial allergies.

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