scholarly journals EVALUATION OF GINGIVAL MICROLEAKAGE IN CLASS II COMPOSITE RESTORATIONS: AN IN VITRO STUDY

2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Ognjenka Janković ◽  
Smiljana Paraš ◽  
Radmila Arbutina ◽  
Irena Kuzmanović Radman ◽  
Tijana Adamović ◽  
...  
Keyword(s):  
Hybrid Composite ◽  
In Vitro Study ◽  
Class Ii ◽  
Control Group ◽  
Agno3 Solution ◽  
Soft Start ◽  
Significant Difference ◽  
Flowable Composites ◽  
Vertise Flow

Introduction: Evaluation of microleakage is important for assessing the success of new restorative materials and methods. The aim of this study was to assess the microleakage of class II restorations with different flowable composites as liners and two different polymerization techniques classic and soft start. Materials and Methods: 40 extracted human premolars teeth with class II cavity preparation medial and distal (80 cavities) were divided into four groups: 1.Vertise Flow (VF)+micro hybrid composite Herculite 2.Surefil SDR Flow (SDRF)+micro-hybrid composite Herculite 3.Tetric Flow (TF)+micro-hybrid composite Herculite 4.control group micro-hybrid composite Herculite. Mesial cavities are polymerized with classic and distal cavity with soft start technique of polymerization. After that, the samples were immersed in 0.5% AgNO3 solution and sectioned into the mesiodistal direction. Using a stereomicroscope (Nikon - Japan), with a magnification of 40x, the gingival microleakage of cavities was examined. Data were analyzed using Fisher's and Student's tests. Results: After using the classical polymerization technique, all three used flowable composites VF+Herculite, SDRF+Herculite, TF+Herculite showed less gingival microleakage than the control group. This difference was statistically significant. After the application of the soft-start technique of polymerization, VF+Herculite and SDRF+Hercules showed a statistically significant reduction in gingival microleakage, while TF+Herculite showed a comparable result with control group, without a statistically significant difference. There was no statistically significant difference between classical and soft start polymerization techniques. Conclusion: Flowable composites in this study have reduced gingival microleakage and can be used as liners in the restoration of II class cavities.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P < .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

scholarly journals Milled versus moulded mock-ups based on the superimposition of 3D meshes from digital oral impressions: a comparative in vitro study in the aesthetic area

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Francesca Cattoni ◽  
Giulia Teté ◽  
Alessandro Mauro Calloni ◽  
Fabio Manazza ◽  
Giorgio Gastaldi ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Test Group ◽  
In Vitro Study ◽  
Mean Value ◽  
Digital Impression ◽  
Control Group ◽  
Minimal Invasiveness ◽  
Significant Difference ◽  
The Aesthetic

Abstract Background Aesthetic porcelain veneers proved to be a long-term reliable prosthetic solution, ensuring minimal invasiveness. The use of veneers requires an adhesive cementation technique, so maintaining as much enamel as possible is to ensure lasting success. A diagnostic mock-up is a key tool that allows a preview of the outcome of the aesthetic restoration: it is obtainable both in an analog and digital way. With the recent developments in impression technology and the ever so fast growing use of CAD-CAM technologies it is useful to understand the pros and cons of either one of these techniques (analog and digital) in order to identify the easier and more convenient workflow in aesthetic dentistry. Methods After taking pictures and impressions of the dental arcs of a patient in need of aesthetic rehabilitation, 52 resin models were produced and a digital drawing of the smile was outlined. Both an analog and a digital wax-up were obtained from two of the 52 models: the latter was obtained using digital impressions and a dedicated software. The analog wax-up was then used to produce 25 matrices that have later been used to mould 25 resin mock-ups using a traditional moulding protocol (Control Group - CG). The digital wax-up was used to mill 25 PMMA mock-ups. Each mock-up, both milled and moulded (total 50), was then laid on the other 50 resin models as a digital impression of it was taken. The STL. files of the milled mock-ups were compared with the 3D CAD wax-up made using a specific software. The STL. files of the analog printed mock-ups were compared with the traditional wax-up design. A statistical analysis was carried out to evaluate the difference between the groups. Results The statistical analysis showed a significant difference (P > 0.01) between the mean value of the distance between the points of the overlapping STL. meshes in GC (0.0468 mm) and in TG (Test Group - TG) (0,0109 mm). Conclusions The study showed a difference in accuracy between traditional moulded and milled mock-ups compared to their original wax-up. The data analysis reports that the digital method allows for greater accuracy. Within the limitations of this study, a fully digital workflow is to considered more reliable when it come to creating an esthetic mockup: the digital procedure has been shown to be more accurate than the one made manually which is much more operator dependent and it brings an increase to the chance of error, and that could ultimately affect the final result.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study

2016 ◽  
Vol 1 (2) ◽  
pp. 56-59
Author(s):  
Natanasabapathy Velmurugan ◽  
Poornima Reddy ◽  
Suma Balla ◽  
Sandhya S Raghu ◽  
Garlapati T Gupta ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Modulus Of Elasticity ◽  
In Vitro Study ◽  
Chlorine Concentration ◽  
Control Group ◽  
Calcium Hypochlorite ◽  
Significant Difference ◽  
Root Dentin

ABSTRACT Introduction The aim of this study was to compare in vitro the effect of 5% calcium hypochlorite [Ca(OCl)2] and 5% sodium hypochlorite (NaOCl) on flexural strength and modulus of elasticity of root dentin. Materials and methods The available chlorine concentration of each solution was determined using iodometric titration. Standardized planoparallel dentin bars (n= 20) were divided into two test groups and one control group. The control, group 1, consisted of dentin bars stored in normal saline. The dentin bars in the two test groups were treated by exposure to following solutions: Group 2 to 5% Ca(OCl)2; and group 3 to 5% NaOCl. All the three test solutions were changed once in 15 minutes for 30 minutes. The dentin bars were then loaded to failure using three-point bend test. Results Available chlorine concentration was 64% in both the test solutions. There was a significant reduction in the flexural strength of 5% NaOCl group compared to 5% Ca(OCl)2-treated ones. A significant difference in modulus of elasticity was observed between the test groups and the control groups and also between the 5% Ca(OCl)2 and 5% NaOCl groups. Conclusion Within the limitations of this study, 5% NaOCl reduced the flexural strength and modulus of elasticity of root dentin bars more when compared to 5% Ca(OCl)2. How to cite this article Reddy P, Balla S, Raghu SS, Velmurugan N, Gupta GT, Sahoo HS. Effect of 5% Calcium Hypochlorite on Mechanical Properties of Root Dentin: An in vitro Study. J Oper Dent Endod 2016;1(2):56-59.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals Comparison of MTA, CEM Cement, and Biodentine as Coronal Plug during Internal Bleaching: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Paridokht Zarean ◽  
Parichehr Zarean ◽  
Arash Ravaghi ◽  
Maryam Zare Jahromi ◽  
Mitra Sadrameli
Keyword(s):  
Root Resorption ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Cem Cement ◽  
Significant Difference

Background. Internal bleaching is a choice of treatment in discolored endodontically treated teeth. Cervical root resorption is one of the important complications of this treatment. A suggested procedure to prevent this type of resorption is using a coronal barrier under the bleaching materials. The aim of the study was to compare the microleakage of mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) cement, and Biodentine. Materials and Methods. In this in vitro study, a total of 60 single canal incisors were included. They were randomly divided into three experimental groups (n = 16), one positive control group (n = 6), and one negative control group (n = 6). Coronal portion of the canals in the experimental groups was sealed with 3 mm of MTA, CEM cement, or Biodentine as a coronal barrier. After 3 days, specimens were bleached. A fresh Enterococcus faecalis suspension was added to the samples. The culture tubes were observed for 45 days, and the daily turbidity was recorded. Statistical analysis was accomplished by the Kaplan–Meier test and SPSS 22. Results. All positive samples showed turbidity, whereas none of the negative samples allowed bacterial leakage. Results showed no significant difference between MTA, CEM cement, and Biodentine groups. ( P value = 0.304, 0.695, and 0.217). The bacterial microleakage for the two groups also did not show significant differences. Conclusions. CEM cement and Biodentine showed promising results as coronal plug, and clinical studies are needed to test these materials with MTA for avoiding microleakage in internal bleaching treatment.

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

MICROLEAKAGE OF CLASS II RESTORATION WITH COMPOSITE INLAY: AN IN VITRO STUDY

2016 ◽  
pp. 28-32
Author(s):  
Xuan Anh Ngoc Ho ◽  
Thien Man Tran
Keyword(s):  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Class Ii ◽  
Vitro Study ◽  
Significant Difference ◽  
Indirect Composite ◽  
Group 2 ◽  
Composite Inlay ◽  
Group 1

Background: Overcoming the obstacles of Class II restoration, especially the microleakage, is a polemic issue. The present study was performed to evaluate the microleakage of Class II restorations using three different techniques. Aims: The aim of this in vitro study was to evaluate the microleakage of Class II restorations using three different techniques. Materials and methods: The study was carried out in the laboratory with paired comparision between groups. Thirty Class II cavities were prepared on extracted non-carious human permanent molars, randomly divided into 3 groups, which were then restored with 3 different methods. Group 1: indirect composite inlay (Tetric N-Ceram) cemented with resin-modified glass ionomer cement (Fuji Plus); Group 2 indirect composite inlay (Tetric N-Ceram) cemented flowable composite (Tetric N-flow); Group 3: direct composite restoration using Tetric N-Ceram. Before immersed to 2% methylene blue solution for 12 hours, all restorations were subjected to thermal cycling (100 cycles 50C – 55 0C). The extent of dye penetration along the gingival wall was assessed using a grade scale from 0 to 3 under 40 times magnification using digital camera Nikon D7000. Results: All types of restorations showed some rate of microleakage. In comparing the three techniques, group 1 demonstrated the significantly higher rate of leakage compared to the others (p<0.05), whereas group 2 and 3 showed no significant difference. Conclusion: Different luting materials have different influences on the microleakage degree along gingival wall of Class II restoration. Key word: composite inlay, class II restoration, microleakage

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