The effect of cartilage decellularized extracellular matrix-chitosan compound on treating knee osteoarthritis in rats

Knee osteoarthritis (KOA) refers to a common disease in orthopaedics, whereas effective treatments have been rarely developed. As indicated from existing studies, chondrocyte death, extracellular matrix degradation and subchondral bone injury are recognized as the pathological basis of KOA. The present study aimed to determine the therapeutic effect of decellularized extracellular matrix-chitosan (dECM-CS) compound on KOA. In this study, rat knee cartilage was decellularized, and a satisfactory decellularized extracellular matrix was developed. As suggested from the in vitro experiments, the rat chondrocytes co-cultured with allogeneic dECM grew effectively. According to the results of the alamar blue detection, dECM did not adversely affect the viability of rat chondrocytes, and dECM could up-regulate the genes related to the cartilage synthesis and metabolism. As reported from the animal experiments, dECM-CS compound could protect cartilage, alleviate knee joint pain in rats, significantly delay the progress of KOA in rats, and achieve high drug safety. In brief, dECM-CS compound shows a good therapeutic effect on KOA.

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Investigation of an injectable gold nanoparticle extracellular matrix

Journal of Biomaterials Applications ◽

10.1177/08853282211051586 ◽

2021 ◽

pp. 088532822110515

Author(s):

Colten Snider ◽

David Grant ◽

Sheila A Grant

Keyword(s):

Extracellular Matrix ◽

Synovial Fibroblasts ◽

X Ray ◽

Decellularized Extracellular Matrix ◽

Intracellular Ros ◽

Murine Fibroblasts ◽

Post Traumatic ◽

Injection Force ◽

20 Nm

Post-traumatic osteoarthritis (PTOA) is a progressive articular degenerative disease that degrades articular cartilage and stimulates apoptosis in chondrocyte cells. An injectable decellularized, extracellular matrix (ECM) scaffold, that might be able to combat the effects of PTOA, was developed where the ECM was conjugated with 20 nm gold nanoparticles (AuNP) and supplemented with curcumin and hyaluronic acid (HA). Porcine diaphragm ECM was decellularized and homogenized; AuNPs were conjugated using chemical crosslinking followed by mixing with curcumin and/or HA. Injection force testing and scanning electron microscopy with energy-dispersive X-ray spectroscopy were utilized to characterize the ECM scaffolds. In vitro testing with L929 murine fibroblasts, equine synovial fibroblasts, and Human Chondrocytes were used to determine biocompatibility, reactive oxygen species (ROS) reduction, and chondroprotective ability. The results demonstrated that conjugation of 20 nm AuNPs to the ECM was successful without significantly altering the physical properties as noted in the low injection force. In vitro work provided evidence of biocompatibility with a propensity to reduce intracellular ROS and an ability to mitigate apoptosis of chondrocyte cells stimulated with IL-1β, a known apoptosis inducing cytokine. It was concluded that an injectable AuNP-ECM may have the ability to mitigate inflammation and apoptosis.

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Liver-derived decellularized extracellular matrix gels induce complex branching and bile ductule network formation of cholangiocytes in vitro

Frontiers in Bioengineering and Biotechnology ◽

10.3389/conf.fbioe.2016.01.00776 ◽

2016 ◽

Vol 4 ◽

Author(s):

Lewis Phillip ◽

Alpini Gianfranco ◽

Green Richard ◽

Shah Ramille

Keyword(s):

Extracellular Matrix ◽

Network Formation ◽

Decellularized Extracellular Matrix ◽

Bile Ductule

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Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial

10.22541/au.163507702.20739142/v1 ◽

2021 ◽

Author(s):

Svenja Nellinger ◽

Ivana Mrsic ◽

Silke Keller ◽

Simon Heine ◽

Alexander Southan ◽

...

Keyword(s):

Tissue Engineering ◽

Extracellular Matrix ◽

Adipose Tissue ◽

Cultured Cells ◽

Structural Characteristics ◽

Human Adipose Tissue ◽

Minimal Invasive ◽

Healthcare Applications ◽

Decellularized Extracellular Matrix

Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively towards their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells (ASCs), towards their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans (sGAGs) compared to cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.

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Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

Journal of Biomaterials Applications ◽

10.1177/08853282211045770 ◽

2021 ◽

pp. 088532822110457

Author(s):

Matthew J Smith ◽

Sandi G Dempsey ◽

Robert W Veale ◽

Claudia G Duston-Fursman ◽

Chloe A F Rayner ◽

...

Keyword(s):

Extracellular Matrix ◽

Hyaluronic Acid ◽

Soft Tissue ◽

Tissue Repair ◽

Matrix Composites ◽

Soft Tissue Repair ◽

Decellularized Extracellular Matrix ◽

Biological Functionality ◽

Traditional Approaches

Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for clinical indications where additional thickness and biomechanical performance are required. However, traditional approaches to the fabrication of multi-layered dECM devices introduce additional laminating materials or chemical modifications of the dECM that may impair the biological functionality of the material. Using an established dECM biomaterial, ovine forestomach matrix, a novel method for the fabrication of multi-layered dECM constructs has been developed, where layers are bonded via a physical interlocking process without the need for additional bonding materials or detrimental chemical modification of the dECM. The versatility of the interlocking process has been demonstrated by incorporating a layer of hyaluronic acid to create a composite material with additional biological functionality. Interlocked composite devices including hyaluronic acid showed improved in vitro bioactivity and moisture retention properties.

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Decellularized Extracellular Matrix for Cancer Research

Materials ◽

10.3390/ma12081311 ◽

2019 ◽

Vol 12 (8) ◽

pp. 1311 ◽

Cited By ~ 14

Author(s):

Takashi Hoshiba

Keyword(s):

Cancer Research ◽

Extracellular Matrix ◽

Cancer Cell ◽

Cancer Progression ◽

Intracellular Signaling ◽

Genetic Mutation ◽

Cell Behavior ◽

Characterization Methods ◽

Decellularized Extracellular Matrix

Genetic mutation and alterations of intracellular signaling have been focused on to understand the mechanisms of oncogenesis and cancer progression. Currently, it is pointed out to consider cancer as tissues. The extracellular microenvironment, including the extracellular matrix (ECM), is important for the regulation of cancer cell behavior. To comprehensively investigate ECM roles in the regulation of cancer cell behavior, decellularized ECM (dECM) is now used as an in vitro ECM model. In this review, I classify dECM with respect to its sources and summarize the preparation and characterization methods for dECM. Additionally, the examples of cancer research using the dECM were introduced. Finally, future perspectives of cancer studies with dECM are described in the conclusions.

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Systems Pharmacology-Based Precision Therapy and Drug Combination Discovery for Breast Cancer

Cancers ◽

10.3390/cancers13143586 ◽

2021 ◽

Vol 13 (14) ◽

pp. 3586

Author(s):

Ze-Jia Cui ◽

Min Gao ◽

Yuan Quan ◽

Bo-Min Lv ◽

Xin-Yu Tong ◽

...

Keyword(s):

Breast Cancer ◽

Drug Discovery ◽

High Throughput Sequencing ◽

High Reliability ◽

Animal Experiments ◽

Common Disease ◽

Biomedical Data ◽

Systems Pharmacology

Breast cancer (BC) is a common disease and one of the main causes of death in females worldwide. In the omics era, researchers have used various high-throughput sequencing technologies to accumulate massive amounts of biomedical data and reveal an increasing number of disease-related mutations/genes. It is a major challenge to use these data effectively to find drugs that may protect human health. In this study, we combined the GeneRank algorithm and gene dependency network to propose a precision drug discovery strategy that can recommend drugs for individuals and screen existing drugs that could be used to treat different BC subtypes. We used this strategy to screen four BC subtype-specific drug combinations and verified the potential activity of combining gefitinib and irinotecan in triple-negative breast cancer (TNBC) through in vivo and in vitro experiments. The results of cell and animal experiments demonstrated that the combination of gefitinib and irinotecan can significantly inhibit the growth of TNBC tumour cells. The results also demonstrated that this systems pharmacology-based precision drug discovery strategy effectively identified important disease-related genes in individuals and special groups, which supports its efficiency, high reliability, and practical application value in drug discovery.

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Highly efficient cardiac differentiation and maintenance by thrombin-coagulated fibrin hydrogels enriched with decellularized porcine heart extracellular matrix

10.1101/2020.01.30.927319 ◽

2020 ◽

Author(s):

Fatemeh Navaee ◽

Philippe Renaud ◽

Thomas Braschler

Keyword(s):

Extracellular Matrix ◽

Collagen I ◽

Cardiac Differentiation ◽

Neonatal Cardiomyocytes ◽

Decellularized Extracellular Matrix ◽

Ventricular Tissue ◽

Key Factor ◽

Myoblast Cell Line ◽

Myoblast Cell

AbstractWe provide a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen for the formation of an in-vitro 3D cardiac cell culture model. Rapid and specific coagulation with thrombin allows gentle inclusion of cells while avoiding sedimentation during formation of the dECM-fibrin composite. We use the system in co-culture with Nor-10 fibroblasts to enhance cardiogenic differentiation of the H9c2 myoblast cell line. The combination of co-culture and appropriate substrate allows to abrogate the use of retinoids, classically considered necessary for cardiogenic H9c2 differentiation. Further enhancement of differentiation efficiency is obtained by 3D embedding. We then proceed with culture of rat neonatal cardiomyocytes in the 3D system. While for H9c2 cells, the collagen content of the dECM was the key factor required for efficient differentiation, the use of dECM-fibrin has specific advantages regarding the culture of neonatal cardiomyocytes. Calcium imaging and analysis of beating motion both indicate that the dECM-fibrin composite significantly enhances recovery, frequency, synchrony and maintenance of spontaneous beating, as compared to various controls including matrigel, pure fibrin and collagen I, but also a fibrin-collagen I blend.

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