Diversity in root growth responses to moisture deficit in young faba bean (Vicia faba L.) plants

Background Soil moisture deficiency causes yield reduction and instability in faba bean (Vicia faba L.) production. The extent of sensitivity to drought stress varies across accessions originating from diverse moisture regimes of the world. Hence, we conducted successive greenhouse experiments in pots and rhizotrons to explore diversity in root responses to soil water deficit. Methods A set of 89 accessions from wet and dry growing regions of the world was defined according to the Focused Identification of Germplasm Strategy and screened in a perlite-sand medium under well watered conditions in a greenhouse experiment. Stomatal conductance, canopy temperature, chlorophyll concentration, and root and shoot dry weights were recorded during the fifth week of growth. Eight accessions representing the range of responses were selected for further investigation. Starting five days after germination, they were subjected to a root phenotyping experiment using the automated phenotyping platform GROWSCREEN-Rhizo. The rhizotrons were filled with peat-soil under well watered and water limited conditions. Root architectural traits were recorded five, 12, and 19 days after the treatment (DAT) began. Results In the germplasm survey, accessions from dry regions showed significantly higher values of chlorophyll concentration, shoot and root dry weights than those from wet regions. Root and shoot dry weight as well as seed weight, and chlorophyll concentration were positively correlated with each other. Accession DS70622 combined higher values of root and shoot dry weight than the rest. The experiment in GROWSCREEN-Rhizo showed large differences in root response to water deficit. The accession by treatment interactions in taproot and second order lateral root lengths were significant at 12 and 19 DAT, and the taproot length was reduced up to 57% by drought. The longest and deepest root systems under both treatment conditions were recorded by DS70622 and DS11320, and total root length of DS70622 was three times longer than that of WS99501, the shortest rooted accession. The maximum horizontal distribution of a root system and root surface coverage were positively correlated with taproot and total root lengths and root system depth. DS70622 and WS99501 combined maximum and minimum values of these traits, respectively. Thus, roots of DS70622 and DS11320, from dry regions, showed drought-avoidance characteristics whereas those of WS99501 and Mèlodie/2, from wet regions, showed the opposite. Discussion The combination of the germplasm survey and use of GROWSCREEN-Rhizo allowed exploring of adaptive traits and detection of root phenotypic markers for potential drought avoidance. The greater root system depth and root surface coverage, exemplified by DS70622 and DS11320, can now be tested as new sources of drought tolerance.

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Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

Bulletin of the National Research Centre ◽

10.1186/s42269-019-0263-y ◽

2020 ◽

Vol 44 (1) ◽

Cited By ~ 1

Author(s):

Mahmoud Ahmed Touny El-Dabaa ◽

Hassan Abd-El-Khair

Keyword(s):

Plant Growth ◽

Faba Bean ◽

Growth Parameters ◽

Dry Weight ◽

Research Centre ◽

Plant Growth Promoting Bacteria ◽

Fresh Weight ◽

Orobanche Crenata ◽

Trichoderma Spp ◽

Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

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Isolation and Assessment of the Symbiotic Efficiency of Faba bean (Vicia faba L.) Nodulating Rhizobial Isolates from Eastern Hararghe Highlands of Ethiopia

Current Agriculture Research Journal ◽

10.12944/carj.9.2.04 ◽

2021 ◽

Vol 9 (2) ◽

pp. 91-105

Author(s):

Mulugeta Mekonnen ◽

Ameha Kebede

Keyword(s):

Vicia Faba ◽

Faba Bean ◽

Dry Weight ◽

Symbiotic Efficiency ◽

Shoot Dry Weight ◽

Nacl Concentration ◽

Vicia Faba L ◽

Positive Correlations ◽

Ph Range ◽

Rhizobial Isolates

This particular work was devoted to isolate and assess the symbiotic efficiency of faba bean (Vicia faba L.)-nodulating rhizobia isolate at few faba bean growing areas of the eastern Hararghe highlands of Ethiopia. Overall 50 rhizobia isolates were obtained from soil samples of three Woredas (districts) of the eastern Hararghe highlands using the host trap method. Out of these 50 isolates, 40 were presumptively identified as rhizobia. Among these 40 rhizobia isolates, only 31 were successful to nodulate faba bean, and authenticated as true faba bean nodulating rhizobia. Concerning the symbiotic efficiency, about 52%, 35%, and 13% of the rhizobial isolates were found to be highly effective, effective, and lowly-effective, respectively. The correlation data on the sand experiment displayed that nodule dry weight was associated positively and significantly (r = 0.494, p<0.05) with shoot dry weight while shoot dry weight was associated positively and significantly (r=0.41, p<0.05) with plant total nitrogen. Positive correlations were also observed concerning shoot dry weight and dry weight of nodules (r = 0.7, p<0.05) on unsterilized soil. Among the observed rhizobium isolates, EHHFR (4A, 6A) showed the highest symbiotic efficiency above 110%, tolerated NaCl concentration ranging from 2% to 6% and 2% to 8%, respectively, and a pH range of 4.5 to 8 and 5 to 8, respectively. Thus, based on their symbiotic efficiency at the greenhouse level and relative tolerance to extreme conditions these faba bean nodulating rhizobia isolates were recommended to be used as nominees for the future development of faba bean rhizobial inoculants after being tested on field conditions.

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Comparison of Root System Morphology of Cucurbit Rootstocks for Use in Watermelon Grafting

HortTechnology ◽

10.21273/horttech04098-18 ◽

2018 ◽

Vol 28 (5) ◽

pp. 629-636 ◽

Cited By ~ 4

Author(s):

Matthew B. Bertucci ◽

David H. Suchoff ◽

Katherine M. Jennings ◽

David W. Monks ◽

Christopher C. Gunter ◽

...

Keyword(s):

Root System ◽

Surface Area ◽

Root Length ◽

Dry Weight ◽

Root Systems ◽

Root Surface ◽

Root Diameter ◽

Root Surface Area ◽

Diameter Class ◽

Main Effect

Grafting of watermelon (Citrullus lanatus) is an established production practice that provides resistance to soilborne diseases or tolerance to abiotic stresses. Watermelon may be grafted on several cucurbit species (interspecific grafting); however, little research exists to describe root systems of these diverse rootstocks. A greenhouse study was conducted to compare root system morphology of nine commercially available cucurbit rootstocks, representing four species: pumpkin (Cucurbita maxima), squash (Cucurbita pepo), bottle gourd (Lagenaria siceraria), and an interspecific hybrid squash (C. maxima × C. moschata). Rootstocks were grafted with a triploid watermelon scion (‘Exclamation’), and root systems were compared with nongrafted (NG) and self-grafted (SG) ‘Exclamation’. Plants were harvested destructively at 1, 2, and 3 weeks after transplant (WAT), and data were collected on scion dry weight, total root length (TRL), average root diameter, root surface area, root:shoot dry-weight ratio, root diameter class proportions, and specific root length. For all response variables, the main effect of rootstock and rootstock species was significant (P < 0.05). The main effect of harvest was significant (P < 0.05) for all response variables, with the exception of TRL proportion in diameter class 2. ‘Ferro’ rootstock produced the largest TRL and root surface area, with observed values 122% and 120% greater than the smallest root system (‘Exclamation’ SG), respectively. Among rootstock species, pumpkin produced the largest TRL and root surface area, with observed values 100% and 82% greater than those of watermelon, respectively. These results demonstrate that substantial differences exist during the initial 3 WAT in root system morphology of rootstocks and rootstock species available for watermelon grafting and that morphologic differences of root systems can be characterized using image analysis.

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Yield losses caused by virus infection in four combinations of non-persistently aphid-transmitted virus and cool-season crop legume

Australian Journal of Experimental Agriculture ◽

10.1071/ea03060 ◽

2004 ◽

Vol 44 (1) ◽

pp. 57 ◽

Cited By ~ 14

Author(s):

L. J. Latham ◽

R. A. C. Jones ◽

B. A. Coutts

Keyword(s):

Virus Infection ◽

Mosaic Virus ◽

Seed Yield ◽

Faba Bean ◽

Seed Weight ◽

Dry Weight ◽

Yield Losses ◽

Cool Season ◽

Individual Seed ◽

Shoot Dry Weight

Field experiments provided quantitative information on the yield losses caused by virus infection within 4 different combinations of non-persistently aphid-transmitted virus and cool-season crop legume: Alfalfa mosaic virus (AMV) in chickpea, faba bean and lentil, and Cucumber mosaic virus (CMV) in lentil. Virus infection foci were introduced into plots and naturally occurring aphids spread infection from these to the other plants. Plants were tagged individually when typical virus symptoms first appeared during the growing period. Paired plant comparisons between symptomatic and asymptomatic plants were made to measure different yield loss parameters. Late infection with AMV in faba bean cv. Fiord diminished shoot dry weight by 41% and seed yield by 45%, but plants infected earlier recovered sufficiently from their initial shock reaction not to produce significant yield losses. In plants of lentil cv. Matilda first showing symptoms at different times, infection with AMV decreased shoot dry weight by 74–76%, seed yield by 81–87% and individual seed weight by 10–21%, while CMV diminished shoot dry weight by 72–81%, seed yield by 80–90% and individual seed yield by 17–25%. Early infection with AMV killed plants of chickpea cv. Tyson while later infection decreased shoot dry weight by 50%, seed yield by 98% and individual seed weight by 90%. The first tentative evidence for seed transmission of AMV in faba bean is reported with a transmission rate of 0.04%.

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Screening of indigenous rhizobacteria as potential biological control against faba bean (Vicia faba L.) gall disease caused by Olpidium viciae

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00483-6 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Alemayehu Dugassa ◽

Tesfaye Alemu ◽

Yitbarek Woldehawariat

Keyword(s):

Faba Bean ◽

Early Treatment ◽

Dry Weight ◽

Phenotypic Traits ◽

Dual Culture ◽

Shoot Dry Weight ◽

Detached Leaves ◽

Moderately Resistant

Abstract Background Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits. Results Pseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety. Conclusions The results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.

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CHLORIMURON-ETHYL IN CONVENTIONAL AND TRANSGENIC SOYBEAN CULTIVARS UNDER WATER DEFICIT STRESS

Revista Caatinga ◽

10.1590/1983-21252018v31n405rc ◽

2018 ◽

Vol 31 (4) ◽

pp. 832-842

Author(s):

Clebson Gomes Gonçalves ◽

Antonio Carlos da Silva Junior ◽

Maynumi Scarano ◽

Maria Renata Rocha Pereira ◽

Dagoberto Martins

Keyword(s):

Root System ◽

Soil Water ◽

Water Deficit ◽

Dry Weight ◽

Water Deficit Stress ◽

Limiting Factor ◽

Soybean Cultivars ◽

Water Potentials ◽

Chlorimuron Ethyl ◽

Soybean Plants

ABSTRACT Water deficit is a limiting factor for the soybean yield; it triggers different physiological and anatomical adaptations that have deleterious effects on the plants and can affect the selectivity of herbicides, causing production losses. In this context, the objective of this work was to evaluate the action of the chlorimuron-ethyl herbicide when applied at different stages of soybean plants, using conventional and transgenic cultivars, and different soil water potentials. A rate of 20 g ha-1 of the chlorimuron-ethyl herbicide was applied to two soybean cultivars (MG/BR46-Conquista - conventional, and BRS-Valiosa-RR - transgenic) at two phenological stages (V2 - first fully expanded trifoliate leaves, and V4 - third fully expanded trifoliate leaves), using three soil water potentials (-0.03 MPa, -0.07 MPa, and -0.5 MPa). Phytotoxicity, and plant height were evaluated at 3, 7, 14, and 21 days after the herbicide application. The shoot dry weight, root dry weight, and root system nodulation were evaluated. The soybean plants had lower phytotoxicity when subjected to application of chlorimuron-ethyl under water deficit conditions. The use of chlorimuron-ethyl reduced the growth and biomass of soybean plants and affected the plants' root system nodulation. The transgenic cultivar (BRS-Valiosa-RR) presented better performance when subjected to a moderate water deficit (-0.07 MPa), which contributes to biological nitrogen fixation.

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Legume-rhizobium specificity effect on nodulation, biomass production and partitioning of faba bean (Vicia faba L.)

Scientific Reports ◽

10.1038/s41598-021-83235-8 ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Bayou Bunkura Allito ◽

Nana Ewusi-Mensah ◽

Vincent Logah ◽

Demelash Kefale Hunegnaw

Keyword(s):

Biomass Production ◽

Faba Bean ◽

Field Experiments ◽

Block Design ◽

Dry Weight ◽

N Fertilizer ◽

Weight Increase ◽

Root Dry Weight ◽

Shoot Dry Weight ◽

Rhizobium Strains

AbstractGreenhouse and multi-location experiments were conducted for two consecutive years to investigate the effects of rhizobium on nodulation, biomass production and partitioning of faba bean. Split-plot in randomized complete block design was used for field experiments. Treatments consisted of six rhizobium strains and three faba bean varieties. Peat carrier-based inoculant of each strain was applied at the rate of 10 g kg−1 seed. Non-inoculated plants without N fertilizer and with N fertilizer served as –N and + N controls, respectively. Data on nodulation, shoot dry weight and root dry weight were collected and analyzed. Inoculation of rhizobium significantly increased nodulation of faba bean under greenhouse and field conditions. Location x strain x variety interaction had significant effects on nodulation, dry matter production and partitioning. Rhizobium inoculation increased nodulation, shoot and root dry weights of faba bean across locations. For example, inoculation with rhizobium strains NSFBR-15 and NSFBR-12 to variety Moti resulted in 206.9 and 99.3% shoot dry weight increase at Abala Gase and Hankomolicha, respectively and 133.3 and 70.7% root dry weight increase on the same variety at the same sites, respectively. Nodulation and biomass production depend on the compatibility between faba bean genotype and rhizobium strain and its interaction with soil bio-physical conditions.

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EFFECT OF BIO-FERTILIZERS AND MOLYBDENUM ON GROWTH AND YIELD OF GREEN BEAN

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v48i3.380 ◽

2017 ◽

Vol 48 (3) ◽

Author(s):

Al-Jebory & Al-Rukabi

Keyword(s):

Block Design ◽

Dry Weight ◽

Root Surface ◽

Growth And Yield ◽

Control Treatment ◽

Root Surface Area ◽

Nitrogen Fixing ◽

Green Beans ◽

Shoot Dry Weight ◽

Level Of Significance

This experiment was carried out at the fields of the Department of Horticulture and Landscape Gardening, College of Agriculture, University of Baghdad, during the spring season of 2015 to study the effect of nitrogen fixing bacterial bio-fertilizers including Rhizobium phaseoli, Azotobacter chroococcum, and Azospirillum brasilense and molybdenum on growth and yield of green beans. The experiment was conducted according to the randomized complete block design (RCBD) with three replications each included 11 treatments represent the interactions between nitrogen fixing bacteria strains, molybdenum solo application, molybdenum and bacteria strains interactions, in addition to the recommended nitrogen application and control treatment. Results were analyzed using the least significant differences (LSD) test at 5% level of significance. Results showed significant effect of the treatment that included all bacteria strains and Mo (T11) on increasing plant macro elements (N, P, and K) which gave 2.39%, 0.49%, and 2.92%, respectively and Fe and Mo concentrations of 269.67 mg.L-1 and 0.69 mg.L-1, respectively and protein percentage in the pods of 13.98%. in addition, treatment of both R. phaseoli and A. chroococcum (T5) gave the most significant main root length of 36.56 cm while R. phaseoli and A. chroococcum and Mo (T9) gave the largest root surface area of 115.00 cm2 and the highest number of root nods that reached 5.33 nod.plant-1. Moreover, T5 significantly increased plant height, leaf number, shoot dry weight, and pod’s length that reached 54.67 cm, 30.22 leaf.plant-1, 65.56 g.plant-1, and 13.62 cm, respectively. As for the T9, results showed significant increase in the number of pods per plant and plant yield of 51.61 pod.plant-1 and 262.03 g.plant-1, respectively.

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266 Retractable Shading Reduces Summer Substrate Temperatures in Container-grown Nursery Crops

HortScience ◽

10.21273/hortsci.34.3.488b ◽

1999 ◽

Vol 34 (3) ◽

pp. 488B-488

Author(s):

Sven E. Svenson

Keyword(s):

Substrate Temperature ◽

Root System ◽

Dry Weight ◽

Douglas Fir ◽

Shoot Dry Weight ◽

Root Death ◽

Southwest Region ◽

Nursery Crops ◽

Substrate Temperatures

The influence of no shading; 30%, 47%, or 63% black polypropylene stationary shading; and white poly retractable shading (50% shade operated to provide morning “cold trapping”) on substrate temperature was studied for Coreopsis verticillata `Zagreb' and Forsythia `Lynwood' growing in 2.75-L black polycontainers filled with an unamended Douglas-fir bark substrate. The southwest region of the rootball had the highest daily substrate temperatures under all the shading systems. Substrate temperatures were highest under no shading or 30% shading (often >45 °C) and lowest under retractable shading (never >38 °C). Root death occurred on the southwest portion of the rootball on plants growing under all shading systems except under retractable shading. Coreopsis and Forsythia were taller when grown under 63% stationary shading compared to other shading systems but had more shoot dry weight when grown under retractable shading. Cooler substrate temperatures that prevent damage to the root system may help explain increased growth of some nursery crops when produced under retractable shading.

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Root Pruning Influences Level of Embolism during Posttransplant Growth of Corylus colurna

HortScience ◽

10.21273/hortsci.31.4.689d ◽

1996 ◽

Vol 31 (4) ◽

pp. 689d-689

Author(s):

P.R. Knight ◽

J.R. Harris ◽

J.K. Fanelli

Keyword(s):

Root System ◽

Leaf Area ◽

Plant Height ◽

Root Length ◽

Dry Weight ◽

Pine Bark ◽

Root Pruning ◽

Branch Number ◽

Shoot Dry Weight

Two-year-old, bareroot, Corylus colurna seedlings were grown in 7.5-L containers from 15 Mar. to 23 June 1995. Plants were grown in a glasshouse using pine bark media. Temperatures were maintained at 30/20°C. Plants received no fertilization or Osmocote 18–6–12 top-dressed at 14 or 28 g/container. Additionally, plants were pruned to remove 0%, 25%, or 50% of the root system based on root length. Height, diameter, branch number, leaf area, and root and shoot dry weight increased linearly as rate of fertilization increased. Percent embolism was not influenced by rate of fertilization. Plant height, branch number, leaf area, and root and shoot dry weight were not influenced by rate of root pruning. Plant diameter increased linearly as rate of root pruning decreased. Percent embolism increased linearly as rate of root pruning increased.

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