scholarly journals Isolation and Assessment of the Symbiotic Efficiency of Faba bean (Vicia faba L.) Nodulating Rhizobial Isolates from Eastern Hararghe Highlands of Ethiopia

2021 ◽  
Vol 9 (2) ◽  
pp. 91-105
Author(s):  
Mulugeta Mekonnen ◽  
Ameha Kebede
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Dry Weight ◽  
Symbiotic Efficiency ◽  
Shoot Dry Weight ◽  
Nacl Concentration ◽  
Vicia Faba L ◽  
Positive Correlations ◽  
Ph Range ◽  
Rhizobial Isolates

This particular work was devoted to isolate and assess the symbiotic efficiency of faba bean (Vicia faba L.)-nodulating rhizobia isolate at few faba bean growing areas of the eastern Hararghe highlands of Ethiopia. Overall 50 rhizobia isolates were obtained from soil samples of three Woredas (districts) of the eastern Hararghe highlands using the host trap method. Out of these 50 isolates, 40 were presumptively identified as rhizobia. Among these 40 rhizobia isolates, only 31 were successful to nodulate faba bean, and authenticated as true faba bean nodulating rhizobia. Concerning the symbiotic efficiency, about 52%, 35%, and 13% of the rhizobial isolates were found to be highly effective, effective, and lowly-effective, respectively. The correlation data on the sand experiment displayed that nodule dry weight was associated positively and significantly (r = 0.494, p<0.05) with shoot dry weight while shoot dry weight was associated positively and significantly (r=0.41, p<0.05) with plant total nitrogen. Positive correlations were also observed concerning shoot dry weight and dry weight of nodules (r = 0.7, p<0.05) on unsterilized soil. Among the observed rhizobium isolates, EHHFR (4A, 6A) showed the highest symbiotic efficiency above 110%, tolerated NaCl concentration ranging from 2% to 6% and 2% to 8%, respectively, and a pH range of 4.5 to 8 and 5 to 8, respectively. Thus, based on their symbiotic efficiency at the greenhouse level and relative tolerance to extreme conditions these faba bean nodulating rhizobia isolates were recommended to be used as nominees for the future development of faba bean rhizobial inoculants after being tested on field conditions.

scholarly journals Selection of efficient rhizobial symbionts for Cratylia argentea in the cerrado biome

2016 ◽  
Vol 46 (9) ◽  
pp. 1594-1600
Author(s):  
Giovanna Moura Calazans ◽  
Christiane Abreu de Oliveira ◽  
José Carlos Cruz ◽  
Walter José Rodrigues Matrangolo ◽  
Ivanildo Evódio Marriel
Keyword(s):  
Nitrogen Concentration ◽  
Dry Weight ◽  
Forage Production ◽  
Bacterial Strains ◽  
Symbiotic Efficiency ◽  
Nodule Number ◽  
Shoot Dry Weight ◽  
Productive System ◽  
Selection Of ◽  
Rhizobial Isolates

ABSTRACT: Cratylia argentea is a leguminous shrub native to the cerrado, which has great potential for forage production and recovery of degraded areas. This study aimed to isolate, characterize, and select efficient rhizobial strains in symbiosis with Cratylia argentea . Rhizobacteria were isolated from the nodules of 12-month-old plants and cultivated in pots containing cerrado soil. Twenty-five bacterial strains were obtained, which displayed extensive variability with respect to morphological and symbiotic characteristics. Cratylia argentea seeds were planted in pots containing 5kg of cerrado soil and maintained in the greenhouse. The treatments consisted of 25 rhizobial isolates, two controls (without nitrogen and without inoculation), with or without nitrogen fertilization (5mgN·plant-1·week-1), and four replications. Plants were cultivated for 150 days after planting seeds to evaluate nodule number, nodule dry weight, shoot and root dry weight, shoot and root N content, and relative and symbiotic efficiency. Thirteen isolates improved shoot dry weight (up to 65.8%) and shoot nitrogen concentration (up to 76%) compared with those of control treatments. Two isolates, 4 (CR42) and 22 (CR52), conferred higher symbiotic efficiency values of approximately 20%. Therefore, these two rhizobial isolates displayed the highest potential as beneficial inoculants to optimize the symbiotic efficiency for Cratylia and to increase the incorporation of nutrients and biomass into the productive system in the cerrado.

scholarly journals Aqueous Extract of (Eruca sativa Mill) as Growth Stimulant in Enhancing Growth and Yield of Faba Bean (Vicia faba L)

2021 ◽  
Vol 36 (1) ◽  
pp. 57-66
Author(s):  
Amal F Ehtaiwwesh ◽  
Fouziyah Qarimidah
Keyword(s):  
Vicia Faba ◽  
Aqueous Extract ◽  
Faba Bean ◽  
Foliar Spray ◽  
Dry Weight ◽  
Growth And Yield ◽  
Aqueous Extracts ◽  
Eruca Sativa ◽  
Vicia Faba L ◽  
Growth Stimulant

A field study was conducted in the fall of 2019 to investigate the potential of Arugula (Eruca sativa Mill) aqueous extract as a growth stimulant in enhancing the growth and yield of Fa- ba Bean (Vicia faba L). The study was conducted using sandy soil at a farm in Abo Esaa town in a plot size of 3X5 m2 with a row spacing of 25cm,which based on a complete randomized design (RCD) with four replications, three treatments were carried out, including no foliar spray with E. sativa extracts (control) and foliar sprayed with 20% and 40% aqueous extracts of E. sativa. Ac- cordingly, Faba Bean (Vicia faba L) plants were foliar sprayed six times with the aqueous extracts of E. sativa at rates of 20 and 40% at vegetative and reproductive stages. The result showed that among these concentrations, the foliar spray of faba bean plants with 40% of E. sativa extracts po- tentially were increased all measured growth and yield traits. The results pointed out that plant height increased by 32%, number of branches by 73%, number of leaves by 95%, number of seeds plant by 89%, leaves, stems, pods and roots dry weight by 92%, 80%, 74%, and 89%, respectively. Thus, the study concluded that E. sativa aqueous extracts could potentially be used efficiently by crop producers as a growth enhancer for faba bean crops because of their productivity, great nutri- tive value, low cost, and environmentally friendly nature.

Assessment of soil fertility augmenting potential of faba bean (Vicia faba L.) germplasm and their performance

2016 ◽  
Vol 3 (3) ◽  
Author(s):  
Anil Kumar Singh ◽  
Rashmi Yadav ◽  
M.K. Meena ◽  
Y.J. Khan
Keyword(s):  
Soil Fertility ◽  
Vicia Faba ◽  
Faba Bean ◽  
Climate Scenario ◽  
Climatic Conditions ◽  
Third Place ◽  
Changing Climate ◽  
Vicia Faba L ◽  
Unique Ability

Faba bean (Vicia faba L.) maintain third place with respect to area and production among legume. Its unique ability to excel under all most all type of climatic conditions, it is one of the best performing crops under changing climate scenario. Its soil fertility augmenting potential and their performance was evaluated for two years with 73 accessions collected from Bihar. This study provides glimpses of scope and magnitude of soil fertility improving potential of faba bean (Vicia faba L.)

scholarly journals Identification of tolerance to metribuzin and imazethapyr herbicides in faba bean ( Vicia faba L.)

Crop Science ◽  
2021 ◽  
Author(s):  
Lynn Abou Khater ◽  
Fouad Maalouf ◽  
Somanagouda B. Patil ◽  
Rind Balech ◽  
Diana Nacouzi ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Vicia Faba L

scholarly journals Enhanced Abiotic Stress Tolerance of Vicia faba L. Plants Heterologously Expressing the PR10a Gene from Potato

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 173
Author(s):  
Abeer F. Desouky ◽  
Ahmed H. Ahmed ◽  
Hartmut Stützel ◽  
Hans-Jörg Jacobsen ◽  
Yi-Chen Pao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Vicia Faba ◽  
Faba Bean ◽  
Abiotic Stress Tolerance ◽  
Wild Type ◽  
Stress Injury ◽  
Bean Plants ◽  
Vicia Faba L

Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (Vicia faba L.) plants encoding a PR10a gene from potato (Solanum tuberosum L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. PR10a-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na+-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the PR10a gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.

scholarly journals Exogenous Auxin-Mediated Salt Stress Alleviation in Faba Bean (Vicia faba L.)

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 547
Author(s):  
Arafat Abdel Hamed Abdel Latef ◽  
Md. Tahjib-Ul-Arif ◽  
Mohammad Saidur Rhaman
Keyword(s):  
Salt Stress ◽  
Vicia Faba ◽  
Faba Bean ◽  
Stress Responses ◽  
Foliar Application ◽  
Soluble Sugar ◽  
Principal Component ◽  
Vicia Faba L ◽  
Catalase Peroxidase ◽  
Amino Acid Contents

Auxin not only controls the development processes, but also regulates the stress responses of plants. In this investigation, we explored the potential roles of exogenously applied indole-3-acetic acid (IAA) in conferring salt tolerance in the faba bean (Vicia faba L.). Our results showed that foliar application of IAA (200 ppm) to salt-exposed (60 mM and 150 mM NaCl) plants promoted growth, which was evidenced by enhanced root–stem traits. IAA application ensured better osmotic protection in salt-stressed plants which was supported by reduced proline and enhanced soluble sugar, soluble protein, and total free amino acid contents in the roots, stem, and seeds. IAA application also increased the number of nodules in salt-stressed plants, which may facilitate better nitrogen assimilation. Moreover, IAA mediated improvements in mineral homeostasis (K+, Ca2+, and Mg2+) and the translocation of Na+, while it also inhibited excessive accumulation of Na+ in the roots. Salt-induced oxidative damage resulted in increased accumulation of malondialdehyde, whereas IAA spraying relegated malondialdehyde by improving antioxidant enzymes, including superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Together, these results together with a principal component analysis uncovered that foliar spraying of IAA alleviated the antagonistic effects of salt stress via enhancing osmolyte accumulation, ionic homeostasis, and antioxidant activity. Finally, exogenous IAA enhanced the yield of broad beans under high salinity conditions.

Effect of irrigation regimes using saline water on faba bean ( Vicia faba L.) yield and water productivity in an arid environment*

2021 ◽  
Author(s):  
Fathia El Mokh ◽  
Kamel Nagaz ◽  
Ashok Kumar Alva ◽  
Mohamed Moncef Masmoudi ◽  
Netij Ben Mechlia
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Saline Water ◽  
Water Productivity ◽  
Arid Environment ◽  
Irrigation Regimes ◽  
Vicia Faba L

scholarly journals Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Mahmoud Ahmed Touny El-Dabaa ◽  
Hassan Abd-El-Khair
Keyword(s):  
Plant Growth ◽  
Faba Bean ◽  
Growth Parameters ◽  
Dry Weight ◽  
Research Centre ◽  
Plant Growth Promoting Bacteria ◽  
Fresh Weight ◽  
Orobanche Crenata ◽  
Trichoderma Spp ◽  
Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

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