Effects of Nigella sativa Linn. Oil on Gut Bacteria and Liver Function Status of Albino Wistar Rats

Background: Nigella sativa oil (NSO) has been suggested for use in several food and pharmaceutical applications due to its bioactive contents. Objectives: The present study investigated the effects of 14 µl/g body weight dosage of NSO on body weight, gut microflora and liver function status (LFS) of albino wistar rats. Phytochemical analysis of NSO extract was done. Materials and Methods: Sixty male Wistar rats were randomly assigned into two groups: 14 µl/g body weight of NSO was administered to group A while group B was given an equal volume of distilled water. Five rats provided baseline data for weight, the microbial counts and LFS in a 12-weeks experiment. At two weeks interval, five rats were sacrificed from each group and their intestinal contents were used for the microbial count (Viable, Coliform, E. coli, Staphylococci and Lactic acid bacteria (LAB)) assessment and the blood samples for LFS study. Results: Nigella sativa oil showed the presence of alkaloids (0.083 mg/g), flavonoids (0.302 mg/g), saponins (0.325 mg/g), terpenes (0.138 mg/g), steroids (0.152 mg/g), tannins (0.008 mg/g) and terpenoid (0.138 mg/g). In both groups, the weight of rats continued to increase from the onset of the study, but between 10th and 12th week, non-significant (p>0.05) weight reduction was observed from 191.72±3.23 g to 189.30±4.71 g in the treatment group. Baseline Viable, Coliform, E. coli, Staphylococci and LAB counts (x 106 CFU/g) were 160, 146, 55, 23, and 154 respectively. Sequel to intake of NSO for twelve weeks, the microbial counts (x 106 CFU/g) were respectively 49, 38, 27, 11, and 318. Blood samples also showed a significant (p<0.05) reduction in LFS for Aspartate aminotransferase (78.48 to 60.06U/L), Alanine aminotransferase (30.80 to 18.54 U/L), Alkaline phosphatase (97.00 to 79.34U/L), and Bilirubin (0.52 to 0.20 U/L). Conclusion: Beneficial effects of NSO at the investigated dosage of 14 µl/g body weight has been demonstrated as no toxicological effect was observed.

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Nigella sativa oil ameliorates the effects of early weaning on the cerebellum of wistar rats

IBRO Reports ◽

10.1016/j.ibror.2019.09.040 ◽

2019 ◽

Vol 7 ◽

pp. 18

Author(s):

R. Jaji-Sulaimon ◽

M.B. Adekunle ◽

I.T. Gbadamosi ◽

G.O. Omotoso

Keyword(s):

Wistar Rats ◽

Nigella Sativa ◽

Early Weaning ◽

Nigella Sativa Oil

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Preventive Effects of Ginger Extract and Nigella sativa Oil on Anxiety and Depression Behavior in Wistar Rats Exposed to Mercuric Chloride

Pharmacognosy Research ◽

10.5530/pres.14.1.1 ◽

2021 ◽

Vol 14 (1) ◽

pp. 01-04

Author(s):

Sabri Benkermiche ◽

Samir Djemli ◽

Meriem Haloui ◽

Mohamed Lamine Bena-bed ◽

Abdelkrim Tahraoui

Keyword(s):

Mercuric Chloride ◽

Wistar Rats ◽

Nigella Sativa ◽

Anxiety And Depression ◽

Ginger Extract ◽

Nigella Sativa Oil ◽

Preventive Effects

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Motor co-ordinative roles of Nigella sativa oil in mice models of phenol-induced essential tremor

Annals of Health Research ◽

10.30442/ahr.0601-10-70 ◽

2020 ◽

pp. 85-99

Author(s):

RO Folarin ◽

OB Surajudeen ◽

EO Omotosho ◽

AO Owoniyi ◽

DO Oyeleye ◽

...

Keyword(s):

Body Weight ◽

Glutathione Peroxidase ◽

Essential Tremor ◽

Neurological Disorders ◽

Motor Coordination ◽

Nigella Sativa ◽

The Body ◽

Ctrl Group ◽

Nigella Sativa Oil ◽

Different Parts

Background: Essential tremor, regarded as the world's most common movement disorder, is a neuronal disorder characterized by uncontrollable shaking (tremor) of different parts of the body. Nigella sativa is a medicinal herb with pharmacologically proven therapeutic potentials in various ailments including neurological disorders. Objective: To evaluate the therapeutic roles of Nigella sativa on cerebellar phenotypes in phenol-induced mice models of essential tremor. Methods: Tremor response, body weight, temperature, motor coordination (using the parallel bars and static rods tests), relative brain weights, cerebellar glutamate and Glutathione peroxidase (GPX) and histoarchitecture were assayed in 75 adult male BALB/cm ice weighing 21-30g. The animals, equally divided into five groups were respectively administered feed and water only (CTRL); 100mg/kg phenol and 1ml/kg Nigella sativa oil concurrently (PNSC); 100mg/kg phenol (P); 1ml/kg Nigella sativa oil followed by 100mg/kg phenol (NSP); and 1ml/kg Nigella sativa oil (NS). Results: The PNSC, NSP and NS mice displayed significant weight reduction. Histoachitectural defects, stagnancy in weight, high Glutathione peroxidase (GPX) and high glutamate levels and poor motor coordination were exhibited by the P group. The CTRL and NS animals demonstrated good motor coordination while the PNSC and NSP groups showed better coordination than the untreated P group. The CTRL group showed no histoarchitectural defects while the NS and PNSC animals showed histoarchitectural regeneration. Conclusion: This research affirmed the weight-reducing, neuroprotective, neuroregenerative and motor coordinating effects of Nigella sativa in the modelled tremor condition.

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SUB-CHRONIC MPTP-INDUCED PARKINSONISM: SELECTIVE SENSITIVITY OF THE BALB/c MICE AND THE PROPHYLACTIC ROLES OF NIGELLA SATIVA OIL

10.31730/osf.io/v7fu4 ◽

2019 ◽

Author(s):

Royhaan Folarin ◽

Akinola Olonade ◽

Praise Ogunkunle ◽

Philemon Shallie ◽

Thomas Adenowo ◽

...

Keyword(s):

Body Weight ◽

Recognition Memory ◽

Nigella Sativa ◽

Weight Ratio ◽

Striatal Neurons ◽

Object Recognition Test ◽

Nigella Sativa Oil ◽

Neuron Density ◽

Animal Research Ethics ◽

Selective Sensitivity

Parkinsonism is a degenerative neurological syndrome characterised by dopamine deficiency in the basal ganglia. Despite ages of research, there is still lack of holistic and side-effect-free therapy for the disease. Nigella sativa is a multi-potent plant known for its historical and scientifically proven therapeutic potentials in the brain and other body organs. Despite the validity debates on the use of BALB/c mice strains in the modelling of Parkinsonism using MPTP, this study re-appraised the sensitivity versus resistance of the BALB/c mice strain to MPTP and further investigated the possible neuro-thrapeutic role of Nigella sativa oil (NSO) in the Parkinsonic endophenotypes elicited by MPTP in the BALB/c strain. Body weights, relative brain weights, striatal dopamine, striatal neuron density and recognition memory were studied in thirty-two (32) male albino mice, weighing between 18g -25g. They were divided equally into Control (administered with normal feed for 5 days), MPTP (administered with 18mg/kg MPTP i.p for 5 days), NS (administered with 1ml/kgbw NSO p.o. for 5 days), and NS+MPTP (administered with 1ml/kgbw NSO p.o. followed by 18mg/kg MPTP i.p for 5 days). Recognition memory was assayed through Novel Object Recognition test (NORT), and the animals were weighed and euthanised 24 hours after last administration. The brains were excised and the striatum assayed neurochemically for dopamine and illustrated histologically for neuronal density using the H&E stain. Parkinsonic traits such as mild tremor, significant down-regulation of dopamine and striatal neurons (p<0.05) were recorded in the BALB/c mice administered with MPTP only, confirming MPTP-sensitivity for these features. However, significant increase (p<0.05) in appetite, body weight, brain-body weight ratio, and recognition memory was also recorded in the MPTP-administered mice, though Nigella sativa was significantly prophylactic against the negative Parkinsonic features, and ‘moderative’ of the up-regulations induced by MPTP. While this suggests selective MPTP sensitivity and resistance in BALB/c strains, this study recommends the investigation of possible beneficial potentials of MPTP as observed. The research was conducted in conformance with the Animal Research Ethics Committee (AREC) guidelines of the Olabisi Onabanjo University.

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The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

BIOEDUPAT: Pattimura Journal of Biology and Learning ◽

10.30598/bioedupat.v1.i2.pp45-49 ◽

2021 ◽

Vol 1 (2) ◽

pp. 45-49

Author(s):

Syahran Wael ◽

Didik Wahyudi ◽

Tayeb Wael ◽

Zaid Mohamed Jaber

Keyword(s):

Wistar Rats ◽

Nigella Sativa ◽

Cellular Damage ◽

Control Group ◽

Control Group Design ◽

Black Cumin ◽

Group Design ◽

Nigella Sativa Oil ◽

Post Test ◽

Male Wistar Rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67Â±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33Â±13.292) compare in the treatment 250 mg/kgbw (40.67Â±17.512) and 150 mg/kgbw (30.67Â±8.165). avarage value of count sperm in the control (130.83Â±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67Â±23.480) compare in the treatment 250 mg/kgbw (187.50Â±74.538) and 150 mg/kgbw (140.83Â±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.

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HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i11.21854 ◽

2017 ◽

Vol 9 (10) ◽

pp. 225

Author(s):

Nadhira M. Dinar ◽

Sheila Pratiwi ◽

Rain Kihara ◽

Nadia G. Paramita ◽

Nazilla R. Fathurrahman ◽

...

Keyword(s):

Body Weight ◽

New Zealand ◽

Oryctolagus Cuniculus ◽

Nigella Sativa ◽

Treated Group ◽

Nigella Sativa Oil ◽

Oral Group ◽

Nephroprotective Activity ◽

Per Oral ◽

New Zealand Rabbits

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

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Antidiabetic effect of Nigella sativa compared with metformin on blood glucose levels in streptozotocin induced diabetic albino wistar rats

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20211016 ◽

2021 ◽

Vol 10 (4) ◽

pp. 361

Author(s):

Neeraj Sadiq ◽

Ghulam Subhani ◽

Syeda Ayesha Fatima ◽

M. Nadeem ◽

Shaima Zafer ◽

...

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Side Effects ◽

Blood Sugar ◽

Wistar Rats ◽

Islet Cells ◽

Histopathological Examination ◽

Nigella Sativa ◽

Hypoglycemic Effect ◽

Standard Drug

Background: Antidiabetic drugs are many but all of them have adverse effects ranging from simple side effects to serious side effects like hypoglycemic coma. We need alternative drugs which will not only lower blood sugar level but have fewer side effects. One such daily use ingredient in our food is Nigella sativa (kalonji) which has many properties.Methods: This was an objective study in an experimental animal. We divided albino wistar rats into six groups each group with six rats. Diabetes was induced using drug streptozotocin. Three doses of Nigella sativa 0.5 ml, 1 ml, 1.5 ml orally per rat were used. Metformin (100 mg/kg body weight) was used as standard drug. Blood sugar was estimated using glucometer on day 0 (starting day of treatment), day 5, day 10, day 20, day 40. The effect of Nigella sativa was compared with metformin group using the Anova test. Statistical analysis was done using SPSS software version 20.Results: Nigella sativa (NS) produced significant hypoglycemic effect. NS in the dose of 0.5 ml, 1 ml and 1.5 ml produced significant reduction in blood glucose in comparison to day 0. Metformin also (100 mg/kg body weight) produced significant reduction in blood glucose on day 20 and day 40. Comparison of hypoglycemic effect of Nigella sativa is not significantly different on day 10 (1 ml) and day 20 (1.5 ml) in comparison to Metformin (100 mg/kg body weight). Histopathological examination showed that there was partial regeneration of beta islet cells of pancreas by 1.5 ml of Nigella sativa which were damaged due to streptozotocin treatment.Conclusions: Alternative method of treatment for diabetes is very much needed and the study shows the use of the spice (Nigella sativa) daily can lower the blood sugar levels and can serve as an alternative treatment of diabetes mellitus.

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An experimental study of albino rats fed on Nigella sativa and aspirin for four weeks duration: determination of coagulation profile

Annals of King Edward Medical University ◽

10.21649/akemu.v10i2.1178 ◽

2016 ◽

Vol 10 (2) ◽

Author(s):

Akhtar Munir ◽

Muhammad Tayyib ◽

Muhammad Farooq ◽

Muhammad Mashraf ◽

Anjum Rashid ◽

...

Keyword(s):

Experimental Study ◽

Body Weight ◽

Nigella Sativa ◽

Control Group ◽

Albino Rats ◽

Clot Retraction ◽

Blood Samples ◽

Synthetic Diet ◽

Group I

Ninety albino rats were selected and were divided into six groups on the basis of different diets given. Control group (I) was fed on synthetic diet and experimental groups (IIA, IIB, IIC, IID and IIE) were fed on 1 mg aspirin, 15mg, 30 mg, 45 mg Nigella Sativa per kg body weight respectively while HE was given 30 mg NS and 1 mg aspirin/kg body wt. Blood samples were collected by heart puncture and Coagulation parameters were done. BT was prolonged in groups taking aspirin only. APTT was reduced significantly in groups taking different concentration of NS when compared with control. Percentage of clot retraction was weak significantly in groups taking aspirin only when comparing with other groups.

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To study and compare the effects of aspirin and Nigella Sativa on coagulation profile in Albino Rats for six weeks

Annals of King Edward Medical University ◽

10.21649/akemu.v10i1.1154 ◽

2016 ◽

Vol 10 (1) ◽

Author(s):

Akhtar Munir ◽

Muhammad Tayyib ◽

Tahira Tasneem ◽

Muhammad Farooq ◽

Muhammad Ashraf ◽

...

Keyword(s):

Body Weight ◽

Bleeding Time ◽

Nigella Sativa ◽

Control Group ◽

Albino Rats ◽

Clot Retraction ◽

Blood Samples ◽

Synthetic Diet ◽

Coagulation Profile ◽

Group 1

Ninety albino rats were selected and were divided into six groups on the basis of different diets given. Control group (1) was fed on synthetic diet and experimental groups (11A, 11B, 11C, 11D and 11E) were fed on 1 mg aspirin, 15mg, 30mg, 45mg Nigella Sativa per kg body weight respectively while 11E was given 30 mg NS and 1 mg aspirin/kg body wt. Blood samples were collected by heart puncture and different tests were done. Bleeding time was shortened significantly in groups taking N.S. when compared with control. BT was prolonged in groups taking aspirin only. APTT was reduced significantly in groups taking different concentration of NS when compared with control. Percentage of clot retraction was weak significantly in groups taking aspirin only when comparing with other groups.

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