Motor co-ordinative roles of Nigella sativa oil in mice models of phenol-induced essential tremor

Background: Essential tremor, regarded as the world's most common movement disorder, is a neuronal disorder characterized by uncontrollable shaking (tremor) of different parts of the body. Nigella sativa is a medicinal herb with pharmacologically proven therapeutic potentials in various ailments including neurological disorders. Objective: To evaluate the therapeutic roles of Nigella sativa on cerebellar phenotypes in phenol-induced mice models of essential tremor. Methods: Tremor response, body weight, temperature, motor coordination (using the parallel bars and static rods tests), relative brain weights, cerebellar glutamate and Glutathione peroxidase (GPX) and histoarchitecture were assayed in 75 adult male BALB/cm ice weighing 21-30g. The animals, equally divided into five groups were respectively administered feed and water only (CTRL); 100mg/kg phenol and 1ml/kg Nigella sativa oil concurrently (PNSC); 100mg/kg phenol (P); 1ml/kg Nigella sativa oil followed by 100mg/kg phenol (NSP); and 1ml/kg Nigella sativa oil (NS). Results: The PNSC, NSP and NS mice displayed significant weight reduction. Histoachitectural defects, stagnancy in weight, high Glutathione peroxidase (GPX) and high glutamate levels and poor motor coordination were exhibited by the P group. The CTRL and NS animals demonstrated good motor coordination while the PNSC and NSP groups showed better coordination than the untreated P group. The CTRL group showed no histoarchitectural defects while the NS and PNSC animals showed histoarchitectural regeneration. Conclusion: This research affirmed the weight-reducing, neuroprotective, neuroregenerative and motor coordinating effects of Nigella sativa in the modelled tremor condition.

Download Full-text

Protective Effect of Silybum marianum and Nigella sativa Oil Extracts against Cisplatin Induced Nephrotoxicity in Mice

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.18:171-175 ◽

2018 ◽

Vol 18 (2) ◽

pp. 171-175

Author(s):

Ashraf A. ◽

Hassan F. ◽

Batool S. ◽

Nadeem M. ◽

Irshad M. ◽

...

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Petroleum Ether ◽

Nigella Sativa ◽

Histological Study ◽

The Body ◽

Cellular Damage ◽

Elevated Blood ◽

Silybum Marianum ◽

Elevated Blood Glucose

The objective of present study was to analyze and compare the nephroprotective effects of petroleum ether extracts of seeds of Nigella sativa and Silybum marianum in Swiss albino mice. Petroleum ether extracts of seeds of Nigella sativa and Silybum marianum were administered (200 mg kg–1) orally for 14 days following one dose of cisplatin injection (16 mg kg–1 i.p.) on 4th day. Cisplatin toxicity was identified in mice by loss of body weight, reduced triglycerides levels, elevated blood glucose, blood urea nitrogen and creatinine levels (P < 0.05). Silybum marianum and Nigella sativa significantly enhanced the body weight (9%) of diabetic rats. Silybum marianum had significantly reduced the elevated blood glucose (–106%), and BUN levels (–9%) as compared to the Nigella sativa administration. Silybum marianum extract also significantly elevated the levels of triglycerides (257%) which were much reduced after the cisplatin injection. Histological study showed that the cisplatin treated group demonstrated the necrosis signs in the glomerulus of Bowman’s capsule, hyalinization and severe cellular damage. The animals administered with extracts showed nearly normal kidney architecture, however, the kidney tissues of Silybum marianum-treated animals showed some improvement with slight signs of cellular damages.

Download Full-text

SUB-CHRONIC MPTP-INDUCED PARKINSONISM: SELECTIVE SENSITIVITY OF THE BALB/c MICE AND THE PROPHYLACTIC ROLES OF NIGELLA SATIVA OIL

10.31730/osf.io/v7fu4 ◽

2019 ◽

Author(s):

Royhaan Folarin ◽

Akinola Olonade ◽

Praise Ogunkunle ◽

Philemon Shallie ◽

Thomas Adenowo ◽

...

Keyword(s):

Body Weight ◽

Recognition Memory ◽

Nigella Sativa ◽

Weight Ratio ◽

Striatal Neurons ◽

Object Recognition Test ◽

Nigella Sativa Oil ◽

Neuron Density ◽

Animal Research Ethics ◽

Selective Sensitivity

Parkinsonism is a degenerative neurological syndrome characterised by dopamine deficiency in the basal ganglia. Despite ages of research, there is still lack of holistic and side-effect-free therapy for the disease. Nigella sativa is a multi-potent plant known for its historical and scientifically proven therapeutic potentials in the brain and other body organs. Despite the validity debates on the use of BALB/c mice strains in the modelling of Parkinsonism using MPTP, this study re-appraised the sensitivity versus resistance of the BALB/c mice strain to MPTP and further investigated the possible neuro-thrapeutic role of Nigella sativa oil (NSO) in the Parkinsonic endophenotypes elicited by MPTP in the BALB/c strain. Body weights, relative brain weights, striatal dopamine, striatal neuron density and recognition memory were studied in thirty-two (32) male albino mice, weighing between 18g -25g. They were divided equally into Control (administered with normal feed for 5 days), MPTP (administered with 18mg/kg MPTP i.p for 5 days), NS (administered with 1ml/kgbw NSO p.o. for 5 days), and NS+MPTP (administered with 1ml/kgbw NSO p.o. followed by 18mg/kg MPTP i.p for 5 days). Recognition memory was assayed through Novel Object Recognition test (NORT), and the animals were weighed and euthanised 24 hours after last administration. The brains were excised and the striatum assayed neurochemically for dopamine and illustrated histologically for neuronal density using the H&E stain. Parkinsonic traits such as mild tremor, significant down-regulation of dopamine and striatal neurons (p<0.05) were recorded in the BALB/c mice administered with MPTP only, confirming MPTP-sensitivity for these features. However, significant increase (p<0.05) in appetite, body weight, brain-body weight ratio, and recognition memory was also recorded in the MPTP-administered mice, though Nigella sativa was significantly prophylactic against the negative Parkinsonic features, and ‘moderative’ of the up-regulations induced by MPTP. While this suggests selective MPTP sensitivity and resistance in BALB/c strains, this study recommends the investigation of possible beneficial potentials of MPTP as observed. The research was conducted in conformance with the Animal Research Ethics Committee (AREC) guidelines of the Olabisi Onabanjo University.

Download Full-text

HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i11.21854 ◽

2017 ◽

Vol 9 (10) ◽

pp. 225

Author(s):

Nadhira M. Dinar ◽

Sheila Pratiwi ◽

Rain Kihara ◽

Nadia G. Paramita ◽

Nazilla R. Fathurrahman ◽

...

Keyword(s):

Body Weight ◽

New Zealand ◽

Oryctolagus Cuniculus ◽

Nigella Sativa ◽

Treated Group ◽

Nigella Sativa Oil ◽

Oral Group ◽

Nephroprotective Activity ◽

Per Oral ◽

New Zealand Rabbits

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

Download Full-text

EFFECTS OF NIGELLA SATIVA AND SUNFLOWER OIL DIET ON WEIGHT OF ALBINO RATS

The Professional Medical Journal ◽

10.29309/tpmj/2011.18.03.2388 ◽

2011 ◽

Vol 18 (03) ◽

pp. 530-534

Author(s):

MUHAMMAD ANWAR BURIRO ◽

MUHAMMAD TAYYAB ◽

ALLAH DTTTA

Keyword(s):

Body Weight ◽

Bile Salt ◽

Sunflower Oil ◽

High Fat Diet ◽

Baseline Level ◽

Nigella Sativa ◽

The Body ◽

Albino Rats ◽

High Fat ◽

Control Groups

Objective: To study the effects of nigella sativa and sunflower oil on weight of albino rats. Study Design: Experimental study. Period: 1996 to 1997. Setting: PGMI, Lahore. Material & Methods: A study was conducted to determine the effects of nigella sativa and sunflower oil diet intake on body weight in albino rats. Fifty six albino rats with equal number of males and females were selected for the study, they were divided into four different groups, Control groups I and III were given diet (20%), high fat diet supplemented with bile salt (1% colic acid) and antithyroid drug (0.5% propylthiouracil).The experimental group II and IV were given the above diets with supplemented nigella sativa. The high fat diet when given at different intervals increased the body weight as compared to baseline level. Results: The high fat diet when given at different intervals increased the body weight as compared to baseline level. The high fat diet with propylthiouracil and bile salt also increased the body weight and the increase was more as compared to previous groups. The supplements of nigella sativa in the groups decreased body weight significantly as compared to the control groups, which was significantly increased in all the experimental groups as compared to the control groups. Conclusions: These observation confirm that nigella sativa decreased body weight. The above effects will be beneficial for patient with obesity. On the basis of these findings conclusions are made, that nigella sativa has got body weight reducing effects. Both nigella sativa and sunflower oil have got low atherogenic index (TC/HDL) and may be recommended in hyperlipidaemic obese patients or normal individuals. Nigella sativa has got weight reducing potential thus affecting obesity and may indirectly prevent atherosclerosis. Nigella sativa is curative remedy for all diseases except death, is the saying of our Holy Prophet Hazrat Muhammad Sallallaho Alaihe Wasalam.

Download Full-text

Effects of Nigella sativa Linn. Oil on Gut Bacteria and Liver Function Status of Albino Wistar Rats

Asian Food Science Journal ◽

10.9734/afsj/2019/v13i130093 ◽

2019 ◽

pp. 1-7

Author(s):

F. Oluwafemi ◽

P. Ojo ◽

A. L. Kolapo ◽

S. O. Oluwalana

Keyword(s):

Body Weight ◽

Liver Function ◽

Wistar Rats ◽

Nigella Sativa ◽

Phytochemical Analysis ◽

Microbial Count ◽

Blood Samples ◽

E Coli ◽

Microbial Counts ◽

Nigella Sativa Oil

Background: Nigella sativa oil (NSO) has been suggested for use in several food and pharmaceutical applications due to its bioactive contents. Objectives: The present study investigated the effects of 14 µl/g body weight dosage of NSO on body weight, gut microflora and liver function status (LFS) of albino wistar rats. Phytochemical analysis of NSO extract was done. Materials and Methods: Sixty male Wistar rats were randomly assigned into two groups: 14 µl/g body weight of NSO was administered to group A while group B was given an equal volume of distilled water. Five rats provided baseline data for weight, the microbial counts and LFS in a 12-weeks experiment. At two weeks interval, five rats were sacrificed from each group and their intestinal contents were used for the microbial count (Viable, Coliform, E. coli, Staphylococci and Lactic acid bacteria (LAB)) assessment and the blood samples for LFS study. Results: Nigella sativa oil showed the presence of alkaloids (0.083 mg/g), flavonoids (0.302 mg/g), saponins (0.325 mg/g), terpenes (0.138 mg/g), steroids (0.152 mg/g), tannins (0.008 mg/g) and terpenoid (0.138 mg/g). In both groups, the weight of rats continued to increase from the onset of the study, but between 10th and 12th week, non-significant (p>0.05) weight reduction was observed from 191.72±3.23 g to 189.30±4.71 g in the treatment group. Baseline Viable, Coliform, E. coli, Staphylococci and LAB counts (x 106 CFU/g) were 160, 146, 55, 23, and 154 respectively. Sequel to intake of NSO for twelve weeks, the microbial counts (x 106 CFU/g) were respectively 49, 38, 27, 11, and 318. Blood samples also showed a significant (p<0.05) reduction in LFS for Aspartate aminotransferase (78.48 to 60.06U/L), Alanine aminotransferase (30.80 to 18.54 U/L), Alkaline phosphatase (97.00 to 79.34U/L), and Bilirubin (0.52 to 0.20 U/L). Conclusion: Beneficial effects of NSO at the investigated dosage of 14 µl/g body weight has been demonstrated as no toxicological effect was observed.

Download Full-text

Olfacto-protective roles of Nigella sativa oil in Harmaline-induced essential tremor modelling

Annals of Health Research ◽

10.30442/ahr.0602-11-84 ◽

2020 ◽

Vol 6 (2) ◽

pp. 218-229

Author(s):

R Folarin ◽

S John ◽

O Oyenuga ◽

N Tijani ◽

O Otulana ◽

...

Keyword(s):

Essential Tremor ◽

Nigella Sativa ◽

Brain Weight ◽

Dopamine Level ◽

Negative Effects ◽

Concurrent Administration ◽

Nigella Sativa Oil ◽

Histological Data ◽

Pre Treatment ◽

Average Size

Background: Harmaline is a tremorgenic beta-carboline, reported to induce acute postural and kinetic tremor. Essential Tremor (ET) is an idiopathic slowly neurodegenerative tremor disorder which also compromises olfactory acuity. Nigella sativa (NS) is a therapeutic agent widely used in the treatment of various ailments. Objective: To determine the effect of NSon olfactory functions of mice treated with harmaline. Methods: Seventy-five BALB/c male mice weighing 20g-25g, were equally divided into five groups, namely CNTRL (received only Normal saline), NS (received NS oil1ml/kg), HML(received Harmaline 20mg/kg), HNS (received Harmaline and Nigella sativa concurrently), and NSH (received NSfollowed by Harmaline). Olfactory sensitivity and discrimination were assayed through buried food test. The olfactory bulb was assayed neurochemically for glutamate and dopamine, and histologically for neuronal architecture using haematoxylin and eosin stain. Differences in neurochemical and histological data, body weight, appetite, relative brain weight, sensitivity and discrimination indices were statistically analysed. Results: NS was significantly protective against the negative effects of Harmaline. It also effected quick olfactory discrimination, increased dopamine level, decrease in weight difference and increased food consumption in the animals. However, Harmaline increased relative brain weight and GPX levels. The concurrent administration aided in the reduction of neuronal density while neuronal average size reduced on pre-treatment with NS. Conclusion: Harmaline did not induce tremor in the animals, though it resulted in histological and neurochemical deficits. However, it resulted in olfactory insensitivity and indiscrimination, both of which were prevented and ameliorated by Nigella sativa oil.

Download Full-text

Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in the digestive tract of rabbits

Czech Journal of Animal Science ◽

10.17221/1659-cjas ◽

2009 ◽

Vol 54 (No. 8) ◽

pp. 376-386 ◽

Cited By ~ 25

Author(s):

M. Pogány Simonová ◽

A. Lauková ◽

Ľ. Chrastinová ◽

V. Strompfová ◽

Š. Faix ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Body Weight ◽

Glutathione Peroxidase ◽

Enterococcus Faecium ◽

Blood Parameters ◽

The Body ◽

Probiotic Properties ◽

Glutathione Peroxidase Activity ◽

E Coli ◽

Coagulase Positive Staphylococci

The effect of Enterococcus faecium CCM7420, bacteriocin-producing strain with probiotic properties and its partially purified bacteriocin PPB CCM7420 on growth performance, microflora, Eimeria sp. oocysts, biochemical blood parameters and glutathione-peroxidase activity in rabbits was determined. An increase in the body weight of rabbits (P < 0.01) was achieved after E. faecium CCM7420 application. A non-significant reduction of faecal E. coli (including haemolytic E. coli), coagulase-positive staphylococci (CPS) and Staphylococcus aureus was found in rabbits administered the E. faecium CCM7420 strain and lower (non-significant) counts of S. aureus and Clostridium-like sp. were detected in PPB CCM7420 group, compared to the control. In the caecum, a significant reduction of CPS was noted in both experimental groups (P < 0.001 and P < 0.05 for EG1 and EG2, respectively). Biochemical blood parameters increased in both experimental groups (P < 0.05 and P < 0.001 for EG2 and EG1, respectively). In the CCM7420 group, the lowest activity of glutathione-peroxidase was measured (P < 0.001). After the application of PPB CCM7420 (P < 0.05; day 21), a reduction of Eimeria sp. oocysts was recorded.

Download Full-text

Effect Of The Aqueous Extract Of Nigella Sativa Seeds On Blood Serum Glucose, Cholesterol and Total Protein In · Alloxan-Induced Diabetic Rabbits

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v27i1.1094 ◽

2003 ◽

Vol 27 (1) ◽

pp. 27-40

Author(s):

Ekklass H. Al-Assede ◽

Kahtan A. Almzaien

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Aqueous Extract ◽

Total Protein ◽

Nigella Sativa ◽

Serum Glucose ◽

The Body ◽

Diabetic Group ◽

Diabetic Rabbits ◽

Group D

The effect of five weeks oral administration of the aqueous extract of Nigella Sativa Seeds (320 mg/kg body weight) on body weight, blood glucose, cholesterol and total protein (albumin and globulin) concentrations in alloxan induced diabetic rabbits was studied. Twenty-four mature male rabbits were randomly assigned into three groups of eight each. Diabetes was induced experimentally by intravenous injection of alloxan monohydrate (100 mg/kg body weight) into two groups; diabetic group (D), diabetic group which was orally administrated aqueous extract of Nigella Sativa seeds (DN) while the third group served as control(C). The results showed that the aqueous extract of Nigella Sativa seeds significantly reduced (P<0.01) the blood glucose and cholesterol concentrations in group (DN) and significantly increased the concentration of total protein (albumin and globulin), also inhibited the body weight reduction as compared with diabetic group (D) and the control (C). These results revealed the capability of the extract of Nigella Sativa seeds to ameliorate the biochemical changes induced by alloxan in diabetic rabbits.

Download Full-text

The influence of ademetionine on the state of prooxidantantioxidant balance in rats with glycerol-induced acute kidney failure

Medical and Clinical Chemistry ◽

10.11603/mcch.2410-681x.2015.v17.i3.5047 ◽

2015 ◽

Vol 17 (3) ◽

Author(s):

I. I. Zamorskyi ◽

V. M. Drachuk ◽

O. M. Horoshko

Keyword(s):

Renal Failure ◽

Body Weight ◽

Acute Renal Failure ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

The State ◽

The Body ◽

Glutathione Peroxidase Activity ◽

Ceruloplasmin Level ◽

Protein Peroxidation

The influence of ademetionine (heptral) on the state of prooxidant-antioxidant balance in rats’ kidneys and blood under the conditions of glycerol (rhabdomyolytic) acute renal failure was studied in the experiment. It is established, that intraperitoneal ademetionine injection in the dose of 20 mg/kg of the body weight normalizes the prooxidant-antioxidant balance indices, which manifested by a decrease of lipid and protein peroxidation intensity, increase catalase and glutathione peroxidase activity аnd increase of the ceruloplasmin level down to the indices of the control animals.

Download Full-text

SnapKi—An Inertial Easy-to-Adapt Wearable Textile Device for Movement Quantification of Neurological Patients

Sensors ◽

10.3390/s20143875 ◽

2020 ◽

Vol 20 (14) ◽

pp. 3875

Author(s):

Ana Oliveira ◽

Duarte Dias ◽

Elodie Múrias Lopes ◽

Maria do Carmo Vilas-Boas ◽

João Paulo Silva Cunha

Keyword(s):

Neurological Disorders ◽

Motor Impairment ◽

The Body ◽

Use Case ◽

Neurological Patients ◽

Smart Garments ◽

Conductive Textile ◽

Different Parts ◽

Deep Brain ◽

Inertial Unit

The development of wearable health systems has been the focus of many researchers who aim to find solutions in healthcare. Additionally, the large potential of textiles to integrate electronics, together with the comfort and usability they provide, has contributed to the development of smart garments in this area. In the field of neurological disorders with motor impairment, clinicians look for wearable devices that may provide quantification of movement symptoms. Neurological disorders affect different motion abilities thus requiring different needs in movement quantification. With this background we designed and developed an inertial textile-embedded wearable device that is adaptable to different movement-disorders quantification requirements. This adaptative device is composed of a low-power 9-axis inertial unit, a customised textile band and a web and Android cross application used for data collection, debug and calibration. The textile band comprises a snap buttons system that allows the attachment of the inertial unit, as well as its connection with the analog sensors through conductive textile. The resulting system is easily adaptable for quantification of multiple motor symptoms in different parts of the body, such as rigidity, tremor and bradykinesia assessments, gait analysis, among others. In our project, the system was applied for a specific use-case of wrist rigidity quantification during Deep Brain Stimulation surgeries, showing its high versatility and receiving very positive feedback from patients and doctors.

Download Full-text