scholarly journals Derivatizing Reagents for Detection of Organic Compounds By HPLC

2021 ◽  
pp. 1-13
Author(s):  
Pooja T. Chhanikar ◽  
Krishna R. Gupta ◽  
Milind J. Umekar
Keyword(s):  
Fluorescence Detection ◽  
Sulfonyl Chloride ◽  
Phenyl Isocyanate ◽  
Fluorescent Detection ◽  
Chemical Derivatization ◽  
Or Groups ◽  
Highly Sensitive ◽  
Simple Chemical ◽  
Derivatizing Agent ◽  
Uv Visible

Derivatization is the process of chemically modifying a compound to develope a new compound which has properties that are suitable for analysis using HPLC. Derivatization improves the detectability of a target analyte by reaction with suitable derivatizing agent. Derivatization reactions are simple chemical modification of substance that make it compatible with the selected separation method or transforms substance with a low UV- absorption into highly sensitive product. Derivatization reactions in liquid chromatography modify the solutes adding a chomophore for easy UV detection or a fluorophore for sensitive fluorescent detection. The chemical structure of the compound remains same and just modifies the specific functional group for reacting compounds to derivative of deviating chemical and physical properties in order to make them detectable. Introduction of certain elements or groups through chemical derivatization may enhance the detector’s response helpful for the elucidation of structure of analytes. In conclusion, the present review describe various derivatization reagents for pre-column and post column derivatization process in HPLC by UV-visible and fluorescence detection are summarized along with reactions and some practical aspects. The commonly used derivatizing reagents in HPLC are 1-fluoro-2, 4-dinitrobenzene, ninhydrine, 4-N-N-dimethylaminoazobenzene-4’-sulfonyl chloride, benzoyl chloride, phenyl isocyanate for UV-visible detection and o-phthalaldehyde, fluorescamine, 1-dimethylaminonapthalene-5-sulfonyl chloride (DNS-Cl), 9-fluorenylmethyl chloroformate (Fmoc-Cl), benzofurans for Fluorescence detection.

scholarly journals Anchoring zinc-doped carbon dots on a paper-based chip for highly sensitive fluorescence detection of copper ions

The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6297-6305
Author(s):  
Qinglan Miao ◽  
Ji Qi ◽  
Yuanyuan Li ◽  
Xinxia Fan ◽  
Dongmei Deng ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Carbon Dots ◽  
Active Sites ◽  
Copper Ions ◽  
Fluorescent Detection ◽  
Zn Doping ◽  
Highly Sensitive ◽  
Doped Carbon Dots

A novel paper-based chip that anchored zinc-doped carbon dots was constructed for sensitive and stable fluorescent detection of Cu2+. Zn doping increased the active sites for simplifying the modification of carbon dots.

scholarly journals THE DEVELOPMENT OF TEST SYSTEMS FOR DETERMINING THE EXPRESSION OF THE BIRC5 AND HER-2/NEU GENES IN LABORATORY ANIMALS USING PCR WITH FLUORESCENCE DETECTION AND EVALUATION OF THEIR EFFECTIVENESS

2021 ◽  
Vol 20 (2) ◽  
pp. 65-70
Author(s):  
V.M. Semenov ◽  
◽  
V.V. Pabiarzhyn ◽  
E.S. Pashinskaya ◽  
S.K. Egorov ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Detection Method ◽  
Test System ◽  
Fluorescent Detection ◽  
Laboratory Animals ◽  
Test Systems ◽  
Highly Sensitive ◽  
Her 2 ◽  
The Given ◽  
Pcr Test

Objectives. To develop primers and probes for detecting the expression of the BIRC5 and HER-2/neu genes and to optimize the reactions with the subsequent creation of PCR test systems with a fluorescent detection method. Material and methods. Achieving this goal included 5 stages, as a result of which two test systems were developed to determine the expression of the BIRC5 and HER-2/neu genes by real-time PCR in the experiment. Results. Two test systems for determining the expression of the BIRC5 and HER-2/neu genes in laboratory animals by means of PCR with fluorescence detection have been developed, that are highly sensitive and effective. Conclusions. The developed test system can be used for determining changes in the expression of the given genes during experiments of biological, veterinary and medical profiles.

scholarly journals UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF OSELTAMIVIRPHOSPHATE IN PURE AND PHARMACEUTICAL FORMULATION

2020 ◽  
pp. 111-114 ◽  
Author(s):  
SACHIN A. YANJANE ◽  
SHRISHAIL M. GHURGHURE ◽  
VINOD K. MATOLE
Keyword(s):  
Spectrophotometric Method ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Formulation ◽  
Ich Guidelines ◽  
Highly Sensitive ◽  
Double Beam ◽  
Recovery Study ◽  
Uv Visible ◽  
Oseltamivir Phosphate ◽  
Simple Economic

Objective: A new, simple, economical, precise, sensitive, linear, accurate, rapid UV spectrophotometric method has been developed for the estimation of Oseltamivir Phosphate in pure form and pharmaceutical formulation. Methods: This UV method was developed using Methanol as a solvent. In the present method, the wavelength selected for analysis was 218 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 10-50µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. The % RSD value was found to be less than 2. Conclusion: The developed UV spectrophotometric method was found to be simple, economic, sensitive, easy, accurate, linear, specific and highly sensitive and can be used for routine estimation of Oseltamivir Phosphate.

scholarly journals Highly Sensitive Fluorescent Detection of Acetylcholine Based on the Enhanced Peroxidase-Like Activity of Histidine Coated Magnetic Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim
Keyword(s):  
Magnetic Nanoparticles ◽  
High Sensitivity ◽  
High Specificity ◽  
Choline Oxidase ◽  
Fluorescent Detection ◽  
One Pot ◽  
Site Structure ◽  
Specificity And Sensitivity ◽  
Highly Sensitive ◽  
Peroxidase Mimics

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.

A rapid label- and enzyme-free G-quadruplex-based fluorescence strategy for highly-sensitive detection of HIV DNA

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 206-212 ◽  
Author(s):  
Feng Zhang ◽  
Ling Xiang ◽  
Xianghui Xiao ◽  
Xiaoming Chen ◽  
Chunyan Chen ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Sensitive Detection ◽  
Hiv Dna ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Highly Sensitive Detection

Because rapid and selective methods for HIV detection are urgently needed, herein, a simple label- and enzyme-free strategy is constructed for fluorescence detection of HIV DNA.

Development of a multi-sugar LC-MS/MS assay using simple chemical derivatization with acetic anhydride

2016 ◽  
Vol 8 (15) ◽  
pp. 3023-3033 ◽  
Author(s):  
Hermes Licea-Perez ◽  
Venkatraman Junnotula ◽  
Sylvia Zohrabian ◽  
Molly Karlinsey
Keyword(s):  
Acetic Anhydride ◽  
Chromatographic Separation ◽  
Chemical Derivatization ◽  
Carbohydrate Analysis ◽  
Simple Chemical

Carbohydrate analysis poses many analytical challenges, in terms of extraction, chromatographic separation, and detection.

scholarly journals Quantitative Analysis of Fluorescence Detection Using a Smartphone Camera for a PCR Chip

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3917
Author(s):  
Jong-Dae Kim ◽  
Chan-Young Park ◽  
Yu-Seop Kim ◽  
Ji-Soo Hwang
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Detection ◽  
High Performance ◽  
Low Cost ◽  
Dna Amplification ◽  
Fluorescent Detection ◽  
Rt Pcr ◽  
Commercial System ◽  
Intensity Changes ◽  
The Difference

Most existing commercial real-time polymerase chain reaction (RT-PCR) instruments are bulky because they contain expensive fluorescent detection sensors or complex optical structures. In this paper, we propose an RT-PCR system using a camera module for smartphones that is an ultra small, high-performance and low-cost sensor for fluorescence detection. The proposed system provides stable DNA amplification. A quantitative analysis of fluorescence intensity changes shows the camera’s performance compared with that of commercial instruments. Changes in the performance between the experiments and the sets were also observed based on the threshold cycle values in a commercial RT-PCR system. The overall difference in the measured threshold cycles between the commercial system and the proposed camera was only 0.76 cycles, verifying the performance of the proposed system. The set calibration even reduced the difference to 0.41 cycles, which was less than the experimental variation in the commercial system, and there was no difference in performance.

Fluorescence Detection of Hydrazine Hydrate Using Carbon Nanodots Synthesized from Mandarin Rind

2019 ◽  
Vol 891 ◽  
pp. 71-77
Author(s):  
Phitsini Suvarnaphaet ◽  
Wattapong Pinyo ◽  
Suejit Pechprasarn ◽  
Naphat Albutt
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Respiratory Tract ◽  
Hydrazine Hydrate ◽  
Fluorescence Detection ◽  
Carbon Nanodots ◽  
Toxic Chemical ◽  
Highly Sensitive ◽  
Pharmaceutical Industries ◽  
Highly Sensitive Detection

Hydrazine hydrate is a highly toxic chemical widely used in agricultural and pharmaceutical industries. Exposure to hydrazine can induce an irritation of respiratory tract, blindness, damage of the DNA and central nervous system. In this paper, we will show the hydrazine hydrate (N2H4) detection using fluorescence carbon nanodots synthesized from mandarin rind, the so-called R-CNDs. Highly sensitive detection can be seen by naked eyes in a fluorescence red-shifting and by analyzing absorption spectra in case of micromolar concentrations of hydrazine hydrate solution.

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