Effect of Ferrous Iron Stress to West Sumatera Paddy Rice (Oryza nivara) Growth on Nutrient Culture Media

Aims: The research aimed to study the response of West Sumatera brown rice growth on nutrient culture media. Study Design: Factorial design with 2 factors in completely randomized design. Place and Duration of Study: The research was conducted in Laboratory of Tissue Culture, Laboratory of Seed Technology and Shade net house, Faculty of Agriculture, Andalas University, Padang, Indonesia from May to July 2019. Methodology: Factorial design with 2 factors in completely randomized design was used in the research. The first factor was brown rice genotypes (Talang Babungo, Sigambiri, Pido Manggih, Labuh Baru, Balingka, Situjuah, Banuhampu, Pulen Talao, Ladang Talamao and Sikarujuik. The second factor was FeSo2 concentrations (0 ppm, 250 ppm, 500 ppm and 750 ppm). The data was analysed using F test 5% and continued by Duncan’s New Multiple Range Test (DNMRT) 5%. Results: The result showed that there was interaction between brown rice genotypes and Fe concentrations to plant height and root dry weight in early growth stage. According the stress sensitivity index, Sigambiri and Ladang Talamao were the tolerant genotypes to high Fe stress and Talang Babungo and Banuhampu were moderately tolerant genotype in high concentration of Fe stress. Conclusion: High Fe concentration affected the brown rice growth. This result can be a consideration to cultivate brown rice in high iron content land.

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Aluminium stress examination of Pasaman local brown rice genotypes in early phase

JERAMI Indonesian Journal of Crop Science ◽

10.25077/jijcs.1.2.16-25.2019 ◽

2019 ◽

Vol 1 (2) ◽

pp. 16-25

Author(s):

Indra Dwipa

Keyword(s):

Early Phase ◽

Brown Rice ◽

Seed Technology ◽

Tolerance Level ◽

Randomized Design ◽

Rice Genotypes ◽

Completely Randomized Design ◽

Stress Examination ◽

Range Test ◽

Plant Length

One of brown rice resistance is tolerant to Aluminium stress. The research was conducted in Seed Technology Laboratory and shade net house of Faculty of Agriculture, Andalas University form March to June 2017. The research aimed to study the tolerance of 6 Pasaman brown rice genotypes to Al stress. Factorial design in Completely Randomized Design was used in this research. The first factor was brown rice genotypes, Sigambiri,LadangTalamau, SikarojukSilomlomPulen and Perbatasan. The second factor was AlCl3 doses, 0 ppm, 5 ppm, 10 ppm, 15 ppm and 20 ppm. The data was analysed by F test and extended by Duncan’s New Multiple Range Test in 5%. The result showed that there was interaction between Al concentration and 6 brown rice genotypes for height of plant, length of leaves. Based on tolerance level, all genotypes were grouped to Moderate-Tolerant level.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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In vitro development of Encyclea cordigera in different concentrations of honey

Ciência Rural ◽

10.1590/0103-8478cr20150046 ◽

2015 ◽

Vol 46 (4) ◽

pp. 590-592 ◽

Cited By ~ 2

Author(s):

Cibele Mantovani ◽

Kathia Fernandes Lopes Pivetta

Keyword(s):

Culture Media ◽

Leaf Length ◽

In Vitro Development ◽

Randomized Design ◽

Sucrose Medium ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Set Up ◽

Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

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SIFAT FISIKO-KIMIA DAN ORGANOLEPTIK CAKE HASIL SUBSTITUSI TEPUNG TERIGU DENGAN TEPUNG SUWEG

AGRICA ◽

10.37478/agr.v5i2.454 ◽

2020 ◽

Vol 5 (2) ◽

pp. 142-152

Author(s):

Jenny E. R. Markus ◽

Yuliana Tandi Rubak ◽

Handy Tomasoei

Keyword(s):

Wheat Flour ◽

Positive Response ◽

Ash Content ◽

Substitution Treatment ◽

Test Results ◽

Friedman Test ◽

Seed Technology ◽

Organoleptic Test ◽

Randomized Design ◽

Completely Randomized Design

This research was conducted in the Seed Technology laboratory and Laboratory of Microbiology, Faculty of Agriculture, University of Nusa Cendana, Kupang, which lasts from September to December 2013 with aims to investigate the influence of substitution of wheat flour with flour suweg the physicochemical properties and organoleptic cake. This study used a completely randomized design (CRD) with 5 treatments, namely substitution suweg flour to wheat flour: 0%, 10%, 20%, 30% and 40%. The parameters analyzed were proximate, softness, porosity, degree of development, test organoleptic colour, flavour, aroma, and texture. Organoleptic data obtained from the 20 panellists and subsequently analyzed by Friedman test, while ANOVA test if there is a real effect then tested further by using DMRT. The results showed that the substitution of different flour suweg provides a very significant effect on water content, fat, ash content, protein, carbohydrate esandtenderness cake. Based on the results of the organoleptic test, cake with flour substitution suweg the colour, aroma and taste of a real significant show, panellists liked the cake substitution suweg 40%, while the texture, the panellists still like to substitution of 40% flour suweg, an assessment of the texture with the highest scores on substitution treatment 10% flour suweg. Organoleptic test and Friedman test results it can be concluded that the cake treatment received a positive response from the panellists was a cake with 40% flour suweg treatment with a total of 66 rankings for colour, aroma as a total ranking of 68, and for a sense of the total ranking of 74. Similarly, some a score on the cake substitution acceptance panellists ranged from 3,1 to 3,4 and the obtained cake was elected with 40% substitution treatment suweg flour.

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EFEKTIVITAS ECENG GONDOK (Echhornia crassipes) DALAM PENYERAPAN KADMIUM (Cd) PADA LEACHATE TPA GUNUNG TUGEL

Molekul ◽

10.20884/1.jm.2011.6.1.87 ◽

2011 ◽

Vol 6 (1) ◽

pp. 25 ◽

Cited By ~ 3

Author(s):

Sri Lestari ◽

Slamet Santoso ◽

Sulastri Anggorowati

Keyword(s):

Factorial Design ◽

Residence Time ◽

Water Hyacinth ◽

Large Percentage ◽

Randomized Design ◽

Open Dumping ◽

Completely Randomized Design ◽

High Level

Leachate is liquid of result organic garbage that contain substance is dissolved and suspension as decomposition by microorganism. Leachate contain material organic with high level and many formed at landfill with open dumping system. Material organic in leachate can be reduced by phytoremediation with water hyacinth (Echhorniacrassipes). The aim of research are know large closure of water hyacinth, long residence time and the best interaction to reduce the levels of material organic in leachate. Method of research was designed experimental based on Completely Randomized Design (CRD) with Factorial Design. The first factor are closing large percentage of water hyacinth (0%, 25%, 50%, 75% and 100%) and the second factor are long residence time (4 days, 5 days and 6 days). Result of research showed that is closing large of 75% is the best treatment to reducing level of Cd that is 29,279%. and long residence time of 6 days is the best treatment to reducing level of Cd that is 27,211%. Interaction closing large of 75% and long residence time of 6 days is the best treatment to reducing level of Cd that is 39,770%

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Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽

10.15575/1344 ◽

2017 ◽

Vol 4 (2) ◽

pp. 97-109

Author(s):

Lamro Purba ◽

Erni Suminar ◽

Denny Sobardini ◽

Wieny Rizky ◽

Syariful Mubarok

Keyword(s):

Tissue Culture ◽

Leaf Number ◽

Shoot Induction ◽

Seed Technology ◽

Randomized Design ◽

Rooting Media ◽

Completely Randomized Design ◽

Four Levels ◽

Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽

10.24198/kultivasi.v19i3.26932 ◽

2020 ◽

Vol 19 (3) ◽

Author(s):

Ega Raisya ◽

Denny Sobardini Sobarna ◽

Anne Nuraini ◽

Syariful Mubarok ◽

Erni Suminar ◽

...

Keyword(s):

Tissue Culture ◽

Fast Time ◽

Endogenous Auxin ◽

Seed Technology ◽

Randomized Design ◽

Process Plants ◽

Number Of Leaves ◽

Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

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Choice of Confounding in the 2k Factorial Design in 2b Blocks

Academic Journal of Applied Mathematical Sciences ◽

10.32861/ajams.55.50.56 ◽

2019 ◽

pp. 50-56

Author(s):

Francis C. Eze

Keyword(s):

Factorial Design ◽

Linear Combination ◽

Incomplete Block ◽

Multiplication Module ◽

Principal Block ◽

Randomized Design ◽

Number Of Factors ◽

Completely Randomized Design ◽

Homogeneous Block ◽

Number Of Treatment

In 2k complete factorial experiment, the experiment must be carried out in a completely randomized design. When the numbers of factors increase, the number of treatment combinations increase and it is not possible to accommodate all these treatment combinations in one homogeneous block. In this case, confounding in more than one incomplete block becomes necessary. In this paper, we considered the choice of confounding when k > 2. Our findings show that the choice of confounding depends on the number of factors, the number of blocks and their sizes. When two more interactions are to be confounded, their product module 2 should be considered and thereafter, a linear combination equation should be used in allocating the treatment effects in the principal block. Other contents in other blocks are generated by multiplication module 2 of the effects not in the principal block. Partial confounding is recommended for the interactions that cannot be confounded.

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Effect of Silica Fertilizer to Growth and Yield of Rice (Oryza sativa L.) in Jajar Legowo Method

Asian Journal of Research in Crop Science ◽

10.9734/ajrcs/2021/v6i130106 ◽

2021 ◽

pp. 1-8

Author(s):

Yulia Sartika ◽

Auzar Syarif ◽

Indra Dwipa

Keyword(s):

Oryza Sativa ◽

Factorial Design ◽

Rice Plant ◽

Rice Field ◽

Oryza Sativa L ◽

Growth And Yield ◽

Single Factor ◽

Randomized Design ◽

Completely Randomized Design ◽

Range Test

Aims: The research aimed to study the interaction between Jajar Legowo method and silica fertilizer doses to growth and yield of rice. Study Design: Factorial design in Completely randomized design Place and Duration of Study: The research was conducted in farmer’s rice field in Linggo Sari Baganti, Pesisir Selatan, West Sumatera, Indonesia from July to Oktober 2020. Methodology: Factorial design with 2 factors in completely randomized design was used in the research. The first factor was Jajar Legowo method that consisted of 3 degrees (2:1, 3:1 dan 4:1) and the second factor was silica fertilizer doses that consisted of 4 degrees (0 ml/L, 5 ml/L, 10 ml/L and 15 ml/L). The data was analysed using F test 5% and continued by Duncan’s New Multiple Range Test (DNMRT) 5% Results: Generally, the interaction between Jajar legowo method and silica fertilizer did not affect the production of rice plant. But, for single factor both jajar legowo method and silica application affected the growth of rice plant Conclusion: The production per hectare of rice plant was lower than description so that this method should be improved to obtain the better result.

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Domestication of Red Seaweed (Gelidium latifolium) in Different Culture Media

ILMU KELAUTAN Indonesian Journal of Marine Sciences ◽

10.14710/ik.ijms.25.1.39-44 ◽

2020 ◽

Vol 25 (1) ◽

pp. 39-44

Author(s):

Andri Wijayanto ◽

Ita Widowati ◽

Tjahjo Winanto

Keyword(s):

Research Laboratory ◽

Culture Medium ◽

Culture Media ◽

Raw Material ◽

Growth Media ◽

Red Seaweed ◽

Randomized Design ◽

Gelidium Latifolium ◽

Domestication Process ◽

Completely Randomized Design

Gelidium latifolium is one of red seaweed types potentially can be developed as an industrial raw material. Since Gelidium is currently taken from ocean, the availability of seaweed from aquaculture is necessary to overcome the small number of its availability in nature. In Indonesia, G. latifolium cultivation has not been carried out so that domestication is required. The use of macro and micro nutrients in growth media is essentially needed for the domestication process. Domestication requires fast media and place for growth. The purpose of this study is to determine the growth of biomass and the survival of G. latifolium in different culture media. The study was conducted in a semi-outdoor research laboratory. The method used in this research is laboratory experimental method and Completely Randomized Design (CRD) with the treatment applied using 3 types of culture media (Urea: Za: TSP) by comparison (A) 100: 50: 50% (2 g.L-1), (B) 75: 75: 50% (2 g.L-1) and (C) 75: 50: 75% (2 g.L-1), with 3 replications. The seaweed was kept in 10 L of water in aeration equipped aquarium and filled with 10 g of G. latifolium on each treatment. The best growth rate of G. latifolium biomass is 5.67± 0.58 g and 100±0% are survived in C culture medium with a concentration of 75% Urea: 50% ZA: 75% TSP (2 g.L-1).

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