scholarly journals Domestication of Red Seaweed (Gelidium latifolium) in Different Culture Media

2020 ◽  
Vol 25 (1) ◽  
pp. 39-44
Author(s):  
Andri Wijayanto ◽  
Ita Widowati ◽  
Tjahjo Winanto
Keyword(s):  
Research Laboratory ◽  
Culture Medium ◽  
Culture Media ◽  
Raw Material ◽  
Growth Media ◽  
Red Seaweed ◽  
Randomized Design ◽  
Gelidium Latifolium ◽  
Domestication Process ◽  
Completely Randomized Design

Gelidium latifolium is one of red seaweed types potentially can be developed as an industrial raw material. Since Gelidium is currently taken from ocean, the availability of seaweed from aquaculture is necessary to overcome the small number of its availability in nature. In Indonesia, G. latifolium cultivation has not been carried out so that domestication is required. The use of macro and micro nutrients in growth media is essentially needed for the domestication process. Domestication requires fast media and place for growth. The purpose of this study is to determine the growth of biomass and the survival of G. latifolium in different culture media. The study was conducted in a semi-outdoor research laboratory. The method used in this research is laboratory experimental method and Completely Randomized Design (CRD) with the treatment applied using 3 types of culture media (Urea: Za: TSP) by comparison (A) 100: 50: 50% (2 g.L-1), (B) 75: 75: 50% (2 g.L-1) and (C) 75: 50: 75% (2 g.L-1), with 3 replications. The seaweed was kept in 10 L of water in aeration equipped aquarium and filled with 10 g of G. latifolium on each treatment. The best growth rate of G. latifolium biomass is 5.67± 0.58 g and 100±0% are survived in C culture medium with a concentration of 75% Urea: 50% ZA: 75% TSP (2 g.L-1).

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

INFLUENCE OF CULTURE MEDIUM ON THE SPORULATION AND VIABILITY OF ASPERGILLUS SPP. AND TALAROMYCES SPP. ENTOMOPATHOGENIC FUNGI

2018 ◽  
Vol 18 (1) ◽  
pp. 12
Author(s):  
Yuyun Fitriana ◽  
Radix Suharjo ◽  
I Gede Swibawa ◽  
Purnomo . ◽  
Puji Lestari ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Culture Medium ◽  
Culture Media ◽  
Spore Production ◽  
Corn Meal ◽  
Spore Viability ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

Influence of Culture Medium on the Sporulation and Viability of Aspergillus spp. and Talaromyces spp. Entomopathogenic Fungi. The purpose of this study was to determine the effect of three kinds of cultures media on the spore production and viability of Aspergillus spp. (AS1, 6, 7, 9) and Talaromyces spp. (AS2–5, 8, 10) entomopathogenic fungi. This study was arranged using Factorial-Completely Randomized Design (CRD) with 2 factors and 3 replications. The first factor was three kinds of cultures media (potato dextrose agar (PDA), corn meal agar (CMA), and sabouraud dextrose agar (SDA)) and the second one was isolates of Aspergillus spp. Or Talaromyces spp.. Data of spore production and spore viability were tested using ANOVA and if there was significantly difference, the data then further analyzed using Tukey‘s Honestly Significant Difference (HSD) test at 5% of significant level. The spore production of Aspergillus spp. were in the range of 0.58 - 14.27 x 108 spores mL-1 (PDA); 0.28 – 2.68 x 108 spores mL-1 (SDA) and 1.85 - 5.33 x 108 spores mL-1 (CMA). The highest spore production was achieved by AS1 isolate that was grown on PDA media. The spore produced by Talaromyces spp. were in the range of 2.15 – 28.62 x108 spores mL-1 (PDA); 0.28 – 29.43 x108 spores mL-1 (SDA); and 1.88 – 16.63 x108 spores mL-1 (CMA). The highest spore production was produced by AS8 isolate which were cultured on PDA. The spore viability among isolates of the two entomopathogenic fungi were not significantly different. The spore viability of Aspergillus spp. was in the range of 95.10 – 97.66% (PDA), 94.02 – 98.45% (SDA) and 92.86 – 98.20% (CMA). The spore viability of Talaromyces spp. was in the range of 95.83 – 100% (PDA), 85.83 – 100% (SDA), and 90.75 – 100% (CMA). Culture medium influenced spore production but not the spore viability. The best culture media used for spore production of both of the entomopathogenic fungi was PDA media.

scholarly journals PENGARUH PENCAMPURAN KOTORAN TERNAK SEBAGAI MEDIA KULTUR TERHADAP PERTAMBAHAN POPULASI CACING TANAH (Lumbricus rubellus)

2019 ◽  
Vol 34 (1) ◽  
pp. 75-80
Author(s):  
Satria Edi Putra ◽  
Iskandar Johan ◽  
Muhammad Hasby
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fish Farming ◽  
Lumbricus Rubellus ◽  
Cattle Dung ◽  
Cow Dung ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Buffalo Dung ◽  
Growing Population

This research aims to determine the effect of mixing cattle dung as a culture medium to the growing population of earthworms. The research was conducted for 42 days starting from 18 October to 28 November 2017 at the fish farming school hall of Agriculture Faculty of Islamic University Riau Pekanbaru. The worm tree used in this study was as many as 960 tail or 80 tail for each container. Worms are maintained for 42 days with different mixtures of medium, i,e, 100% P1 dung treatment, P2 cow-dung treatment 50% + 50% buffalo dung, 50% cow dung + 50% goat dung, 50% cow dung + 50% chicken droppings. The research design was Completely Randomized Design with 4 treatments and 3 replications. The result of this research showed that the increase of population of earthworm with the mixing of cattle dung as culture media which conducted for 42 days is the best treatment of P2 as much as 4266 head/container, then the second one used was in treatment 3 as much as 1855 head/container, and the lowest increase of the population was at treatment P1 as much as 403 head/container, and followed by treatment P4 as much as 575 head/ container.

scholarly journals PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

2020 ◽  
Vol 8 (1) ◽  
pp. 18
Author(s):  
Faisal Ansyarif ◽  
Mursal Ghazali ◽  
Aida Muspiah ◽  
Rina Kurnianingsih
Keyword(s):  
Culture Media ◽  
Positive Control ◽  
Negative Control ◽  
Growth Media ◽  
Extract Concentration ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

scholarly journals Manganese (Mn) and Lead (Pb) Content in Lemna Culture Fertilized by Bioslurry

2020 ◽  
pp. 1-6
Author(s):  
Iskandar . ◽  
Yuli Andriani ◽  
Irfan Zidni
Keyword(s):  
Experimental Method ◽  
Culture Medium ◽  
Culture Media ◽  
Lemna Minor ◽  
Small Water ◽  
Randomized Design ◽  
Water Plant ◽  
Mn Content ◽  
Completely Randomized Design ◽  
Experimental Pond

Lemna minor is a small water plant that floats on water and has the potential to be fresh feed for fish. Bioslurry is a by-product of the management of biogas from livestock manure which is often used as fertilizer for plants because it contains nutrients in a readily available form. Among the contents contained in bioslurry, manganese and lead are important things related to their use for plants and fish. This study aims to determine the mineral (Mn) and lead (Pb) content in the medium of lemna cultivation which is fertilized with various doses of bioslurry. The study was conducted at the Ciparanje Experimental Pond, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia. This study used an experimental method with Completely Randomized Design (CRD) with four treatments with three replications. The treatment in the form of adding bioslurry to the lemna culture media was 0.00%, 0.25%, 1.00% and 2.50% of the volume of water. The minor density of Lemna used is 800 g/m2. The culture was carried out in 4 cycles for 7 days. The parameters observed were Mn and Pb content in the lemna culture medium shortly after the addition of Bioslurry and after 7 days of lemna cultivation. Based on the results of the study, the use of 2.5% bioslurry concentration can produce the highest Mn content with a range of 2.0-4.6 ppm and 2.92 ppm Pb content after 7 days of lemna cultivation. In addition, the use of 2.5% bioslurry concentration resulted in Mn and Pb values in the culture medium shortly after stirring bioslurry in the lemna culture media. Based on the research results, the treatment of adding 2.5% bioslurry to the lemna culture media is the best dose for produced Mn for fish, as well as the safest Pb for aquaculture activities.

Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Denovis Sambode ◽  
Henneke Pangkey ◽  
Sartje Lantu
Keyword(s):  
Population Growth ◽  
Culture Medium ◽  
Quality Parameters ◽  
Organic Fertilizers ◽  
Cow Dung ◽  
Randomized Design ◽  
Period Data ◽  
Growth Development ◽  
Completely Randomized Design ◽  
Medium Culture

The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.

scholarly journals Soybean waste in particleboard production

2018 ◽  
Vol 42 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Evelyn Hoffmamm Martins ◽  
Alan Pereira Vilela ◽  
Rafael Farinassi Mendes ◽  
Lourival Marin Mendes ◽  
Lívia Elisabeth Vasconcellos de Siqueira Brandão Vaz ◽  
...  
Keyword(s):  
Water Absorption ◽  
Water Immersion ◽  
Eucalyptus Grandis ◽  
Raw Material ◽  
Modulus Of Rupture ◽  
High Yield ◽  
Thickness Swelling ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hybrid Eucalyptus

ABSTRACT Brazil is the second largest soybean producer in the world, with a yield of around 96.2 million tons per crop. This high yield leads to a great amount of waste resulting from soybean cultivation, which can reach approximately 41 million tons of waste per year. This material has lignocellulosic properties, which may enable its use as a raw material for particleboard production. Therefore, the objective of this study was to evaluate the use of soybean pods in particleboard production. For particleboard manufacture, wood of the hybrid Eucalyptus urophylla and Eucalyptus grandis was used, added with soybean pods, at proportions of 0%, 25%, 50%, 75% and 100%. For particleboard evaluation, a completely randomized design was used, with five treatments and three replicates, using linear regression and the Scott-Knott test at 5% significance for comparison among the different treatments. The properties apparent density, compaction ratio, water absorption after 2 and 24 hours, thickness swelling after 2 and 24 hours in water immersion, internal bonding, modulus of rupture and modulus of elasticity in bending properties were evaluated. The ratio soybean pod waste and eucalyptus particles in the panels led to an increase in water absorption values and thickness swelling, in addition to a decrease in mechanical properties. The production of panels with approximately 23% soybean pods is feasible.

scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

scholarly journals POTENSI KULIT KOPI SEBAGAI BAHAN BAKU PUPUK KOMPOS DI PROPINSI BENGKULU

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Afrizon
Keyword(s):  
Rice Bran ◽  
Secondary Data ◽  
Raw Material ◽  
Skin Potential ◽  
Coffee Waste ◽  
Randomized Design ◽  
Leather Wastes ◽  
Completely Randomized Design ◽  
Leather Waste ◽  
Fruit Harvest

The experimen of Coffee For Skin Potential Raw Material Compost in Bengkulu Province was conducted from March to June 2010 in the laboratory greenhouse BPTP Bengkulu. The purpose of this study are 1) Testing coffee leather waste into compost in an effort to preserve the environment, 2) Determine the potential of waste as raw material for leather coffee fertilizer in Bengkulu Province. In the manufacture of compost using a completely randomized design (CRD) with 6 treatments and 3 replications. The treatments were: a) 70% waste + 20% KDP coffee cage + 10% rice bran + EM4 + sugar, b) 80% waste + 10% KDP coffee cage + 10% rice bran + EM4 + sugar, c) 80% waste coffee + 20% KDP cage + EM4 + sugar, d) 80% + 20% of coffee waste Manure + EM4, e) 80% waste + 10% KDP coffee cage + 10% rice bran, f) 80% waste + 20 copies % KDP cage. As for seeing the potential of coffee leather waste by gathering primary and secondary data related to commodities which includes the area planted with coffee and productivity in every district in Bengkulu province. The data collected is the percentage of skin waste of coffee per 100 g, area planted with coffee every district, the content of N, P, K and C organic. The research shows that compost with time for 4 weeks has given the maturity of fertilizer as indicated by the value of C / N between 9.75 to 15.99. The composition of 80% waste + 10% coffee hull Manure + 10% rice bran provides the best compost. Compost made from leather wastes contain nutrients coffee in accordance with ISO standards 19-7030-2004 about compost quality standards. Dried coffee fruit harvest yield 51.2% milled coffee leather waste with a potential of 30,222 tons / year. equivalent to 738.323 tons N / yr. This is identical to substitute with an equal amount of urea (46% N) of 339.628 tons / year. equivalent to 86.434 tons P205/th. This is identical to substitute with an equal value superphos (SP-36) amounted to 31.116 tons / yr, equivalent to 876.438 kg K2O/th or identical substitute KCl (60%) amounted to 525.862tons/year.  Keywords : coffee leather waste, compost, potential

Sign in / Sign up

Export Citation Format

Share Document