scholarly journals Cytotoxicity Activity and Phytochemical Screening of Anthocleista djalonensis Root Extracts against Cancer Cells

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Ijeoma Solomon Okoro ◽  
Terrumum Amom Tor-Anyiin ◽  
John Ogbaji Igoli ◽  
Muluh Emmanuel Khan
Keyword(s):  
Ethyl Acetate ◽  
Hela Cells ◽  
Screening Methods ◽  
Anticancer Activities ◽  
Medicinal Uses ◽  
Cytotoxicity Activity ◽  
Anti Cancer ◽  
Pure Compounds ◽  
Acetone Extracts

Aim: Several medicinal uses have been reported for Anthocleista djalonensis and many types of pure compounds have been isolated. However, the anti-cancer activity of this plant has not been proven. The aim of this study was to screen for the phytochemicals present in the rootn-hexane, ethyl acetate, and acetone extracts of Anthocleista djalonensis, and to evaluate its anticancer potential against human cervix adenocarcinoma cells (HeLa cells) in vitro. Place and Duration of Study: The study was carried out in Department of Organic Chemistry, Rhodes University, Grahamstown, South Africa. The duration period was between March and July, 2016. Methodology: Extracts were prepared by soaking the root powder in the respective solvents with continuous stirring; The extracts were filtered and evaporated to remove the solvents. The extracts were then screened for phytocompounds by preliminary screening methods. Anti-cancer potential was carried out by a Resazurin assay and CC50 values were determined. Results: The extracts showed the presence of carbohydrates, glucoside, alkaloids, flavonoids, terpenoids, tannins, saponins, sterols. All extracts demonstrated moderate cytotoxicity against HeLa cells. Conclusion: The hexane, ethyl acetate and acetone extracts showed anticancer property. The roots extracts of Anthocleista djalonesis were thus found to possess potential anticancer activities.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals Antibacterial and anticancer activities of acetone extracts from in vitro cultured lichen-forming fungi

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Agnieszka Felczykowska ◽  
Alicja Pastuszak-Skrzypczak ◽  
Anna Pawlik ◽  
Krystyna Bogucka ◽  
Anna Herman-Antosiewicz ◽  
...  
Keyword(s):  
Anticancer Activities ◽  
Acetone Extracts

scholarly journals In Vitro Antioxidant Activity of Crude Extracts of Harpagophytum Zeyheri and Their Anti-Inflammatory and Cytotoxicity Activity Compared With Diclofenac.

2021 ◽  
Author(s):  
Sibonokuhle F. Ncube ◽  
Lyndy J. McGaw ◽  
Emmanuel Mfotie Njoya ◽  
Hilton G.T. Ndagurwa ◽  
Peter J. Mundy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inflammatory Activity ◽  
In Vitro Assays ◽  
Cytotoxicity Activity ◽  
Tnf Α ◽  
Low Toxicity ◽  
Anti Inflammatory ◽  
Ethyl Acetate Extracts

Abstract Background This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac. Methods In vitro assays were conducted using water, ethanol and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2'-diphenyl-1-picrylhydrazy (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results The ethyl acetate extract had the lowest IC50 values in the DPPH (5.91µg/ml) and ABTS (20.5µg/ml) assay compared to other extracts. Furthermore, the ethyl acetate extracts effectively inhibited NO and TNF-α and proved to be comparable to diclofenac at some concentrations. All extracts of H. zeyheri displayed dose dependent activity and were associated with low levels of human-IL-10 expression compared to quercetin. Furthermore, all extracts displayed low toxicity relative to diclofenac. Conclusions These findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.

scholarly journals In vitro anti-inflammatory and cytotoxicity studies of two mangrove associated lichens, Dirinaria consimilis and Ramalina leiodea extracts

2018 ◽  
Vol 10 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Girija Sastry Vedula
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Agents ◽  
Protein Denaturation ◽  
Cytotoxicity Activity ◽  
Low Degree ◽  
Cytotoxicity Studies ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Cancer Outcome

Plan: To evaluate the anti-inflammatory and cytotoxicity activity of different extracts from two different manglicolous lichens (Dirinaria consimilis and Ramalina leiodea). Preface: Inflammation is the origin of several deadly diseases like cancer, atherosclerosis, Alzheimer’s and rheumatoid arthritis. From decades, lichen extracts and its metabolites are well known in treating inflammation and cancer. Outcome: The outcome of protein denaturation method confirmed that the ethyl acetate and acetone extract of R. leiodea depicted better inhibitory profile against protein denaturation with IC50 values of 268 and 330 µg/mL Furthermore, the results of SRB assay showed that ethyl acetate and acetone extracts of both the lichens acts potently against MCF-7, DLD-1, HeLa and A549. Simultaneously, all the tested extracts depicted the low degree of specificity towards NHME, they are less toxic. Hence, further screening of these extracts may lead to the exploration of safe and potent anti-inflammatory and anticancer agents.

EFEKTIFITAS ANTIKANKER FRAKSI (Curcuma zedoaria) DAN PENGARUHNYA TERHADAP EKSPRESI GEN CASPASE 3 PADA SEL HELA SECARA IN VITRO

2018 ◽  
Vol 1 (2) ◽  
pp. 1
Author(s):  
Apria Wilinda Sumantri
Keyword(s):  
Hela Cells ◽  
Caspase 3 ◽  
Cell Number ◽  
Negative Control ◽  
Hexane Fraction ◽  
Control Group ◽  
Curcuma Zedoaria ◽  
Water Fraction ◽  
Anti Cancer

Aim : The purpose of this research was the evaluate the efficacy of anti-cancer of active fraction of temu putih (Curcuma zedoaria) and their effects on expressetion caspase 3 in Hela cells in vitro Method: Do experimental study in Vitro the research population was whie the Hell cell meanwhile the research sample was HeLa cell that grow normally in cell number of 1 x 104 cell/well. The treatment group was ethanolextract, n-hexane fraction, ethyl acetatefraction and ethanol-water fraction of temu putih (Curcuma zedoaria ) were divided into 6 concentrations, that is 1000, 500, 250, 125, 62,5 dan 31,25 ug/ml; I negative control group ; and the positive control group of cispilatin with a concentration of 200, 100, 50, 25, 12,5 dan 6,25 ug/ml. the data were analyze SPSS Version 20. Result: The research findings showed that the n-hexane fraction of temu putih (Curcuma zedoaria) with the concentration of 154,261 ug/ml has the ability to induce apoptosis of 42.34% and increased the expression of caspase 3of 29,44% in Hell cells. Where as the IC50 Valuve of 20,823 ug/ml. Conclusion : I can be said that the n-hexane fraction has the equivalent ability tp cisplatin 200 mg/ml in inhibiting growth and its effect on the expression of caspase 3 in HeLa cells In Vitro Keywords : N-hexane fraction, Temu putih (Curcuma zedoaria), anti-cancer, HeLa cells, Caspase 3

Study of Antimicrobial Activity of Ethnomedically Important Plant Murraya paniculata

2015 ◽  
Author(s):  
Abhijit Nath ◽  
Mrinal Kanti Bhattacharjee
Keyword(s):  
Methanol Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
No Inhibition ◽  
Murraya Paniculata ◽  
Pure Compounds ◽  
Acetone Extracts ◽  
Scientific Base

Murraya paniculata is a potent ethno medicinally important plant used both in traditional medicine as an analgesic and for wood and its activity against S. aureus established its traditional uses too. Selaginella is also used traditionally in wounds, postpartum, menstrual disease against microbes Lycopodium is used in Rheumatism and disease of lungs and kidneys and our study also revealed the scientific base behind its uses. The result of our experiments showed that in vitro antimicrobial screening of extracts and pure compounds of Murraya paniculata indicated positive activity against Staphylococcus aureus. They showed acetone extracts of the plants showed in inhibition zone of 13mm, methanol extract showed in inhibition zone of 9mm and ethanol extract showed in inhibition zone of 7mm but the distilled water extract showed no inhibition zone. We also performed work on selaginella and lycopodium but got no inhibition zone.

The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols

RSC Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 3260-3268 ◽  
Author(s):  
Maja Krstic ◽  
Marija Stojadinovic ◽  
Katarina Smiljanic ◽  
Dragana Stanic-Vucinic ◽  
Tanja Cirkovic Velickovic
Keyword(s):  
Cervical Cancer ◽  
Green Tea ◽  
Hela Cells ◽  
Cervical Adenocarcinoma ◽  
Anti Cancer ◽  
Cancer Activity

Thein vitroanti-cervical cancer potency of tested polyphenol extracts is exhibited in the following order: green tea > coffee > cocoa, with only green tea showing both pro-oxidative and anti-proliferative action.

scholarly journals POTENTIAL ANTICANCER ACTIVITY IN-VITRO FROM EXTRACT OF Turbinaria decurrens Bory

2020 ◽  
Vol 24 (1) ◽  
pp. 22-24
Author(s):  
Fitriyanti Jumaetri Sami
Keyword(s):  
Ethyl Acetate ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Causes Of Death ◽  
Human Breast ◽  
Cytotoxicity Activity ◽  
Mtt Method ◽  
Diphenyltetrazolium Bromide ◽  
Mcf 7

Cancer is one of the biggest causes of death in the world, not least in Indonesia. Brown algae (T. decurrens Bory) is one of the marine organisms that can be used as a source of natural medicinal ingredients. The purpose of this study was to examine the potential cytotoxicity of extracts of n-hexane, ethyl acetate, and methanol from T. decurrens Bory against MCF-7 human breast cancer cells and H460 lung cancer cells. Samples were extracted using multilevel maceration methods using n-hexane, ethyl acetate, and methanol. Cytotoxicity activity was carried out by the MTT method (3- (4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide). The results showed that all extracts of T. decurrens Bory were potential as anticancer. Ethyl acetate extract can inhibit cancer cells at a concentration of 10 µg / mL with more than 50% cytotoxicity compared to other extracts, both against MCF-7 and H460 cells.

scholarly journals In-Vitro Evaluation of Antibacterial, Antifungal and Anti-HIV Effects of Calophyllum inophyllum Leaf Extract

2020 ◽  
Vol 13 (4) ◽  
pp. 2003-2014
Author(s):  
Arun Kumar ◽  
Yashika Garg
Keyword(s):  
Leaf Extract ◽  
Rapid Development ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Medicinal Uses ◽  
Development Of Resistance ◽  
Pure Compounds ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

Background:Calophylluminophyllum is an evergreen tree with ethno-medical value growing along the seashores and islands of the Pacific and Indian Ocean. All parts of the plant such as bark, seeds and leaves have diverse medicinal uses such as an antiseptic, analgesic in wound healing, astringent, diuretic, purgative and expectorant. Although many species of calophyllum have been studied phytochemically for pharmacological properties, reports on inophyllum species are scanty. Aim of the study:Keeping inview it’s medical importance and availability in India as well as the rapid development of resistance by pathogens to the commonly used synthetic antibiotics,the pharmacological effects of C. inophyllum leaf extract (CIE) on HIV, bacteria and fungi causative of many human diseases was assessed in this study. Material and Methods: Isolation of the pure compounds from the ethanolic CIE was performed by gross column chromatography and tested against lyophilized forms of 8 fungal and 14 bacterial strains grown on Sabouraud’s dextrose agar and nutrient agar media respectively. Fractions and pure compounds isolated from CIE were evaluated against HIV by the HIV-RT inhibition assay by using the RT assay kit(Roche). The results were tabulated and analysed. Results: Among the purified compounds, Inophyllum C & E exhibited significant antibacterial and antifungal properties. Moreover, Inophyllum E was more potent than Inophyllum C in inhibiting the tested strains of bacteria and fungi whereasInophyllum B shows highest antiretroviral activity. Conclusion:We conclude that CIEis aneffective antimicrobial agent against common human pathogens tested in this in-vitro pharmacological evaluation of CIE.

Synthesis of 1-O-acetylbritannilactone analogues from Inula britannica and in vitro evaluation of their anticancer potential

MedChemComm ◽  
2014 ◽  
Vol 5 (10) ◽  
pp. 1584-1589 ◽  
Author(s):  
Jiang-Jiang Tang ◽  
Shuai Dong ◽  
Yang-Yang Han ◽  
Ming Lei ◽  
Jin-Ming Gao
Keyword(s):  
Aromatic Ring ◽  
Hela Cells ◽  
Caspase 3 ◽  
Anticancer Activities ◽  
M Cell ◽  
In Vitro Evaluation ◽  
Inula Britannica ◽  
Induction Of Apoptosis ◽  
Cell Arrest

A series of novel ABL analogues was synthesized by N/O-atom installing and aromatic ring esterifying, and 4a showed in vitro markedly anticancer activities against HeLa cells associated with induction of apoptosis, activation of caspase-3 and G2/M cell arrest.

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