scholarly journals In vitro anti-inflammatory and cytotoxicity studies of two mangrove associated lichens, Dirinaria consimilis and Ramalina leiodea extracts

2018 ◽  
Vol 10 (1) ◽  
pp. 16-26 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Girija Sastry Vedula
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Agents ◽  
Protein Denaturation ◽  
Cytotoxicity Activity ◽  
Low Degree ◽  
Cytotoxicity Studies ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Cancer Outcome

Plan: To evaluate the anti-inflammatory and cytotoxicity activity of different extracts from two different manglicolous lichens (Dirinaria consimilis and Ramalina leiodea). Preface: Inflammation is the origin of several deadly diseases like cancer, atherosclerosis, Alzheimer’s and rheumatoid arthritis. From decades, lichen extracts and its metabolites are well known in treating inflammation and cancer. Outcome: The outcome of protein denaturation method confirmed that the ethyl acetate and acetone extract of R. leiodea depicted better inhibitory profile against protein denaturation with IC50 values of 268 and 330 µg/mL Furthermore, the results of SRB assay showed that ethyl acetate and acetone extracts of both the lichens acts potently against MCF-7, DLD-1, HeLa and A549. Simultaneously, all the tested extracts depicted the low degree of specificity towards NHME, they are less toxic. Hence, further screening of these extracts may lead to the exploration of safe and potent anti-inflammatory and anticancer agents.

scholarly journals Tris-(2-pyridyl)-pyrazolyl Borate Zinc(II) Complexes: Synthesis, DNA/Protein Binding and In Vitro Cytotoxicity Studies

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7341
Author(s):  
Manmath Narwane ◽  
Dorothy Priyanka Dorairaj ◽  
Yu-Lun Chang ◽  
Ramasamy Karvembu ◽  
Yu-Han Huang ◽  
...  
Keyword(s):  
Anticancer Agents ◽  
Binding Mode ◽  
In Vitro Cytotoxicity ◽  
Visible Absorption ◽  
Cytotoxicity Studies ◽  
Ic50 Values ◽  
State Behaviour ◽  
Analytical Tools ◽  
Ct Dna

Zn(II) complexes bearing tris[3-(2-pyridyl)-pyrazolyl] borate (Tppy) ligand (1–3) was synthesized and examined by spectroscopic and analytical tools. Mononuclear [TppyZnCl] (1) has a Zn(II) centre with one arm (pyrazolyl-pyridyl) dangling outside the coordination sphere which is a novel finding in TppyZn(II) chemistry. In complex [TppyZn(H2O)][BF4] (2) hydrogen bonding interaction of aqua moiety stabilizes the dangling arm. In addition, solution state behaviour of complex 1 confirms the tridentate binding mode and reactivity studies show the exogenous axial substituents used to form the [TppyZnN3] (3). The complexes (1–3) were tested for their ability to bind with Calf thymus (CT) DNA and Bovine serum albumin (BSA) wherein they revealed to exhibit good binding constant values with both the biomolecules in the order of 104–105 M−1. The intercalative binding mode with CT DNA was confirmed from the UV-Visible absorption, viscosity, and ethidium bromide (EB) DNA displacement studies. Further, the complexes were tested for in vitro cytotoxic ability on four triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, MDA-MB-468, HCC1937, and Hs 578T). All three complexes (1–3) exhibited good IC50 values (6.81 to 16.87 μM for 24 h as seen from the MTS assay) results which indicated that these complexes were found to be potential anticancer agents against the TNBC cells.

scholarly journals In Vitro Antioxidant Activity of Crude Extracts of Harpagophytum Zeyheri and Their Anti-Inflammatory and Cytotoxicity Activity Compared With Diclofenac.

2021 ◽  
Author(s):  
Sibonokuhle F. Ncube ◽  
Lyndy J. McGaw ◽  
Emmanuel Mfotie Njoya ◽  
Hilton G.T. Ndagurwa ◽  
Peter J. Mundy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inflammatory Activity ◽  
In Vitro Assays ◽  
Cytotoxicity Activity ◽  
Tnf Α ◽  
Low Toxicity ◽  
Anti Inflammatory ◽  
Ethyl Acetate Extracts

Abstract Background This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac. Methods In vitro assays were conducted using water, ethanol and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2'-diphenyl-1-picrylhydrazy (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results The ethyl acetate extract had the lowest IC50 values in the DPPH (5.91µg/ml) and ABTS (20.5µg/ml) assay compared to other extracts. Furthermore, the ethyl acetate extracts effectively inhibited NO and TNF-α and proved to be comparable to diclofenac at some concentrations. All extracts of H. zeyheri displayed dose dependent activity and were associated with low levels of human-IL-10 expression compared to quercetin. Furthermore, all extracts displayed low toxicity relative to diclofenac. Conclusions These findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.

scholarly journals In vitro antioxidant anti inflammatory and cytotoxicity activities from Hexane extract of Bryonopsis laciniosa fruits.

2018 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Sanjeevkumar C.B ◽  
Ramesh L Londonkar ◽  
Umesh Madire Kattegouda
Keyword(s):  
Cell Line ◽  
Potential Application ◽  
Protein Denaturation ◽  
Cancer Cell Line ◽  
Hexane Extract ◽  
Inflammatory Activity ◽  
Cytotoxicity Activity ◽  
Anti Inflammatory ◽  
Mcf 7

<p><em>Bryonopsis laciniosa </em>also known as “Shivlingi” annual climber with bright red fruits and is reported to be highly medicinal in India<em>. </em>As a folk medicine, the plant is used in treatment of broad range of diseases and disorders. In the present study, Hexane extract of <em>B. laciniosa </em>fruits were used to evaluate <em>in</em> <em>vitro</em> anti inflammatory, antioxidant and Cytotoxicity (towards MCF-7 cell line) activities.<em> In vitro</em> anti inflammatory activity by inhibition of protein denaturation, antioxidant assays like DPPH, ABTS, H<sub>2</sub>O<sub>2</sub> and FRAP were used to measure the antioxidant capacity of the hexane extracts and cytotoxicity activity using MCF-7 breast cancer cell line. Hexane extract showed the effective antioxidant activity in all assays compared to ascorbic acid and BHT. The results for<em> In vitro</em> anti inflammatory activity of hexane extract and Dichlofenac drug were equivalent, hexane extract showed promising activity for inhibition of protein denaturation assay. The cytotoxicity activity from hexane extract was noticeable against MCF-7 cell line. The overall results show potential application of <em>Bryonopsis laciniosa </em>fruits suggesting their potential application as a health-promoting functional ingredient or natural preservative in foods.</p>

scholarly journals IN VITRO ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ERANTHEMUM CAPENSE

2019 ◽  
pp. 33-35
Author(s):  
ANOOPA JOHN L ◽  
KANNAPPAN N ◽  
MANOJKUMAR P
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Ethyl Acetate Extract ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Anti Inflammatory

Objective: The present study was aimed to rationalize the scientific basis in traditional use of Eranthemum capense as an antibacterial, antifungal, and anti-inflammatory agent. Methods: Agar well diffusion method is widely used to evaluate the antimicrobial activity of the E. capense aerial part of methanolic and ethyl acetate plant extracts. The same amount (15–20 mL) of Mueller-Hinton agar was poured on glass Petri plates of same size and allowed to solidify. E. capense aerial part of methanolic and ethyl acetate extracts was evaluated in vitro for their anti-inflammatory activities using the bovine serum albumin protein denaturation assay. Results: The result of the study shows that methanolic exract (T3) of the plant, E. capense shows 16 mm zone of inhibition against Pseudomonas fluorescens, while the ethyl acetate extract of the same plant shows 14 mm zone of inhibition against P. fluorescens and E. coli. Hence the methanolic extract of T3 sample shows the antibacterial activity against gram negative bacteria, where as the ethyl acetate extract of T3 shows antibacterial activity against both gram positive and gram negative bacteria. The experimental report revealed that, the methanolic and ethyl acetate extract of the same plant produces zero percentage zone of inhibition against Aspergillus niger and Mucor, hence it does not show any antifungal activity. Conclusion: It is observed that the EA and methanolic extract of E. capense can be used in the treatment of inflammation due to the significant percentage of inhibition of protein denaturation as well as its prove the good antimicrobial agent.

scholarly journals Phytochemical, Proximate, in-vitro Anti-malarial, Anti-inflammatory and Antimicrobial Screening of Leaf Extracts of Combretum platypterum (Welw) Hutch & Dalziel

2021 ◽  
pp. 39-61
Author(s):  
C. E. Anarado ◽  
V. I. E. Ajiwe ◽  
O. F. Obumselu ◽  
C. J. O. Anarado ◽  
N. P. Okafor
Keyword(s):  
Ethyl Acetate ◽  
Protein Denaturation ◽  
Post Partum ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Sexually Transmitted ◽  
Eye Problems ◽  
Anti Inflammatory ◽  
Anti Fungal

Background: Combretum platypterum is used ethno-medically in the treatment  many diseases in Africa, such as; helminthiasis, sexually transmitted diseases, conjunctivitis, malaria, lumps, fever, eye problems, diarrhea, lower backache, coughs, swellings, as febrifuge, tonic, and to stop post-partum bleeding. Aim: This work was aimed at investigating the phytochemical, proximate, anti malarial, anti inflammatory, anti bacterial and anti fungal activities of leaf extracts of Combretum platypterum. Methods: Leaves of C. platypterum were cold extracted with methanol, ethyl acetate and n-hexane. The extracts were subjected to preliminary phytochemical analyses, proximate, anti malarial, anti inflammatory and antimicrobial tests. Results: The results revealed that, alkaloids, terpenoids, flavonoids, cardiac glycosides, carbohydrates, resins and reducing sugars were found in all the extracts. It was also observed that the presence of the metabolites in each extract was in polarity-dependent manner. The percentage composition of carbohydrates was also the highest followed by the composition of proteins. The suppressions of the extracts against P. falciparum at half-maximal inhibitory concentration (IC50 mg/ml) were found to be higher than that of chloroquine at IC50 µg/ml. It was also found that methanol extract had the highest inhibition against Gram positive, Gram negative bacteria and C. albicans in a dose-dependent manner. K. pneumonia was resistant to the inhibition of the extracts. The anti inflammatory assays showed that methanol extracts at 500 mg/ml exhibited higher lipoxygenase Inhibition than diclofenac at 500 µg/ml. Ethyl acetate extract showed highest inhibition of heat-induced hemolysis, protein denaturation and proteinase among all the extracts. Conclusion: The therapeutic use of the leaves of C. platypterum against malaria and swelling was confirmed by the activities of the extracts against inflammation and P. falciparum. It is also necessary that the bioactive compounds responsible for these activities be isolated.

scholarly journals In-vitro anti-inflammatory activity and anti-arthritic activity of ethyl acetate extracts of Skimmia anquetilia leaves

2020 ◽  
pp. 42-44
Author(s):  
Poonam Verma ◽  
Baljinder Singh ◽  
Amarjit Kaur ◽  
Vijender Kumar
Keyword(s):  
Ethyl Acetate ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Maximum Effect ◽  
Membrane Stabilization ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Anti Inflammatory ◽  
Dose Dependent

Current investigations were carried out for the validation of in-vitro anti-inflammatory and anti-arthritic property of leaves of Skimmia anquetilia using red blood cells membrane stabilization and protein denaturation methods respectively. Defatted ethylacetate extracts at different concentration levels (50, 100, 200 and 400 mg/ml) were used in these studies. Dose dependent inhibition of protein denaturation was found 92.41% at 400 mg/ml of extracts and 96.21 % at 100 mg/ml of acetyl salicylic acid as standard in antiarthritic study. Similarly, in membrane stabilization methods, maximum effect found 90.70 % at 400 mg/ml of extracts and 94.88 % at 100 mg/ml of diclofenac sodium as standard for anti-inflammatory evaluation. The results concluded that, ethyl acetate extract of S. anquetilia leaves has shown significant (*aP<0.05) anti-inflammatory and anti-arthritic effects.

Recent Advances in Curcumin Nanocarriers for the Treatment of Different Types of Cancer with Special Emphasis on In Vitro Cytotoxicity and Cellular Uptake Studies

2020 ◽  
Vol 10 (5) ◽  
pp. 577-590
Author(s):  
Jai B. Sharma ◽  
Shailendra Bhatt ◽  
Asmita Sharma ◽  
Manish Kumar
Keyword(s):  
Cancer Cells ◽  
Cellular Uptake ◽  
Anticancer Agents ◽  
Targeted Delivery ◽  
In Vitro Cytotoxicity ◽  
Curcumin Nanoparticles ◽  
Cytotoxicity Studies ◽  
Delivery Technique ◽  
Different Types

Background: The potential use of nanocarriers is being explored rapidly for the targeted delivery of anticancer agents. Curcumin is a natural polyphenolic compound obtained from rhizomes of turmeric, belongs to family Zingiberaceae. It possesses chemopreventive and chemotherapeutic activity with low toxicity in almost all types of cancer. The low solubility and bioavailability of curcumin make it unable to use for the clinical purpose. The necessity of an effective strategy to overcome the limitations of curcumin is responsible for the development of its nanocarriers. Objective: This study is aimed to review the role of curcumin nanocarriers for the treatment of cancer with special emphasis on cellular uptake and in vitro cytotoxicity studies. In addition to this, the effect of various ligand conjugated curcumin nanoparticles on different types of cancer was also studied. Methods: A systematic review was conducted by extensively surfing the PubMed, science direct and other portals to get the latest update on recent development in nanocarriers of curcumin. Results: The current data from recent studies showed that nanocarriers of curcumin resulted in the targeted delivery, higher efficacy, enhanced bioavailability and lower toxicity. The curcumin nanoparticles showed significant inhibitory effects on cancer cells as compared to free curcumin. Conclusion: It can be concluded that bioavailability of curcumin and its cytotoxic effect to cancer cells can be enhanced by the development of curcumin based nanocarriers and it was found to be a potential drug delivery technique for the treatment of cancer.

Curcumin: Natural Antimicrobial and Anti Inflammatory Agent

2021 ◽  
pp. 1-8
Author(s):  
Pehlivanović Belma ◽  
Čaklovica Kenan ◽  
Lagumdžija Dina ◽  
Omerović Naida ◽  
Žiga Smajić Nermina ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Protein Denaturation ◽  
Dose Range ◽  
In Vitro Assays ◽  
Natural Antimicrobial ◽  
In Vivo Models ◽  
Vitro Assay ◽  
Inflammatory Agent ◽  
Anti Inflammatory

The pursuance of novel antimicrobial and anti-inflammatory agents has been expanding due to a significant need for more efficient pharmacotherapy of various infections and chronic diseases. During the last decade, pharmacokinetics, pharmacodynamics and pharmacological properties of curcumin have been extensively studied. The aim of the present study was to evaluate the antibacterial activity of curcumin against both Gram-positive and Gram-negative bacteria as well as its antifungal activity by using in vitro agar well diffusion assay. Moreover, the anti-inflammatory activity of curcumin was determined with in vitro assay of inhibition of protein denaturation. Results demonstrated wide antimicrobial activity of curcumin upon all of the test bacteria and fungi. The strongest activity of curcumin was observed at a concentration of 0.50 mg/ml against S. aureus, L. monocytogenes, E. coli, P. aeruginosa and C. albicans, resulting in a maximum zone of inhibition of 14.7 mm, 14.3 mm, 13.7 mm, 10.7 mm and 10.7 mm, respectively. Findings suggested that the antimicrobial activity of curcuminis dependent upon the concentrations. Furthermore, results demonstrated high effectiveness of curcumin compared to standard acetylsalicylic acid in inhibiting heat-induced protein denaturation, which activity is also depended upon the concentrations. The present study emphasises the potential application of curcumin as a natural antimicrobial and anti-inflammatory agent. However, findings of this study are restricted to in vitro assays and consideration should be given to conducting a study involving wider dose range test substances as well as including further research on in vivo models.

scholarly journals Preliminary phytochemical screenings and Pharmacological activities of Blumea lacera (Burn.f.) DC.

2015 ◽  
Vol 13 (1) ◽  
pp. 69-73
Author(s):  
Md Abul Khair ◽  
Mohammed Ibrahim ◽  
Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Crude Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Acetyl Salicylic Acid ◽  
Antipyretic Activity ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Blumea Lacera

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

scholarly journals In vitro Antidiabetic, anti-obesity and antioxidant proprities of Rosemary extracts

2014 ◽  
Vol 10 (2) ◽  
pp. 2305-2316 ◽  
Author(s):  
Manel Ben Ali ◽  
Kais Mnafgui ◽  
Abdelfattah Feki ◽  
Mohamed Damak ◽  
Noureddine Allouche
Keyword(s):  
Essential Oil ◽  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Antioxidant Properties ◽  
Methanolic Extracts ◽  
Ic50 Values ◽  
Inhibitory Potential ◽  
Prevention Of Diabetes ◽  
First Time

Diabetes mellitus is a serious health problem worldwide that has adverse and long-lasting consequences for individuals, families, and communities. Hence, this study sought to investigate the inhibitory potential of rosemary extracts on key-enzymes related to diabetes such as α-amylase and pancreatic lipase activities, as well as to assess their antioxidant properties in vitro. The IC50 values of Rosemary essential oil, ethyl acetate and methanolic extracts against α-amylase were 28.36, 34.11 and 30.39 µg/mL respectively, and those against pancreatic lipase were 32.25, 36.64 and 34.07 µg/mL, suggesting strong anti-diabetic and anti-obesity effects of Rosemary. The methanolic extract was found to be the highest in levels of phenolic (282.98 µgGAE/mg extract) and flavonoids (161.05 µg QE /mg extract) contents as well as in the antioxidant activity (IC50 = 15.82 µg/mL) as compared to other extracts ethyl acetate (IC50 = 32.23 µg/mL) and essential oil  (IC50 = 96.12 µg/mL).Antioxidant efficacy of Rosemary extracts has been estimated in the stabilization of sunflower oil (SFO) at three different concentrations, i.e. 200 (SFO-200), 500 (SFO-500) and 1000 ppm (SFO-1000). Results showed the highest efficiency of SFO-1000.The results obtained in this study demonstrated for the first time that Rosemary is a potent source of natural inhibitors of α-amylase and pancreatic lipase with powerful antioxidants proprieties that might be used in the food stabilization and the prevention of diabetes and obesity complications as a complementary pharmacological drug.

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