In-vitro Anti-lipoxygenase and Membrane Stabilization Activity of Crude Extracts of Euphorbia lateriflora (Schum and Thonn)

Aims: This study was aimed at evaluating the in-vitro anti-inflammatory potential of crude extracts of Euphorbia lateriflora. Place of Study: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Methodology: The in-vitro anti-inflammatory activity of aqueous and ethanol extracts of E. lateriflora were evaluated via anti-lipoxygenase and red cell membrane stabilization assays using spectrophotometric technique. Results: The aqueous and ethanol extracts demonstrated significant anti-lipoxygenase activity with the aqueous extracts (IC50: 0.34 mg/ml) and the ethanol extracts (IC50: 0.25 mg/ml), showing comparable activity with standard indomethacin (0.27 mg/ml). Contrary to what was observed in the anti-lipoxygenase study, the aqueous extract demonstrated a better membrane stabilization activity with IC50 (1.13 mg/ml) than the ethanol extracts (5.44 mg/ml). The standard diclofenac drug had an IC50 of 1.02 mg/kg. Both the aqueous and the ethanol extracts demonstrated significant anti-inflammatory activity. Conclusion: Observation from this study established the considerable anti-inflammatory potential of this plant by inhibiting lipoxygenase activity and stabilization the membrane of the red blood cells.

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Phytochemical, In vitro Anti-inflammatory and Antimicrobial Potential of Hugonia mystax L.

Research Journal of Pharmacognosy and Phytochemistry ◽

10.52711/0975-4385.2021.00028 ◽

2021 ◽

pp. 169-173

Author(s):

K.P. Jaiganesh ◽

T.J. Jasna ◽

A.C. Tangavelou

Keyword(s):

Tamil Nadu ◽

Diclofenac Sodium ◽

Inflammatory Activity ◽

Phytochemical Analysis ◽

Membrane Stabilization ◽

Traditional System ◽

Antimicrobial Potential ◽

Biochemical Compounds ◽

Anti Inflammatory

Hugonia mystax L., (Linaceae), is commonly distributed in the thorny scrubs and tropical dry evergreen forests of Tamil Nadu, which has been valued for centuries in traditional system of medicine for the treatment of various ailments. In the present study was an attempt to investigate the phytochemical nature and anti-inflammatory, antimicrobial potential by adopting suitable methods. Phytochemical analysis of Hugonia mystax L., plant extracts revealed the presence of various biochemical compounds such as alkaloids, flavonoids, glycosides, triterpenoids and saponins etc. Since triterpenoids and flavonoids have remarkable anti-inflammatory activity, so our present work aims at evaluating in vitro anti inflammatory activity of Hugonia mystax L., by HRBC membrane stabilization method. The inhibition of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The percentage of membrane stabilization for ethanolic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization of Hugonia mystax L., extracts was found to be 94.97 % at a dose of 2000 μg/ml. Therefore, our studies support the isolation and the use of active constituents from Hugonia mystax L., in treating inflammations.

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ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i5.32512 ◽

2019 ◽

pp. 278-281

Author(s):

RAJESH A ◽

DOSS A ◽

TRESINA PS ◽

MOHAN VR

Keyword(s):

Methanol Extract ◽

Protein Denaturation ◽

In Vitro Models ◽

Inflammatory Activity ◽

Membrane Stabilization ◽

Standard Drug ◽

Inflammatory Agent ◽

Potential Source ◽

Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

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A scientific validation of In vitro anti-inflammatory activity of Punica granatum L. by human red blood cell membrane stabilization

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20182830 ◽

2018 ◽

Vol 6 (7) ◽

pp. 2430

Author(s):

Karunakar Kota ◽

Sandhya Sharma ◽

Jameela Tahashildar

Keyword(s):

Methanolic Extract ◽

Punica Granatum ◽

Inflammatory Activity ◽

Membrane Stabilization ◽

Fruit Peel ◽

Standard Drug ◽

Membrane Lysis ◽

Anti Inflammatory ◽

Punica Granatum L

Background: In recent years there has been growing interest in therapeutic use of natural products, especially those derived from plants. P. granatum is very common dietary ingredient in many parts of India and has remarkable biological and medicinal properties.Methods: In the present study, the methanolic extract of fruit peels of Punica granatum Linn. (MEPG) were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac.Results: The results of the study demonstrated that P. granatum contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis.Conclusions: It is concluded that methanolic extract of P. granatum fruit peel possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.

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POTENTIAL ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL EVALUATION OF EXTRACTS OF LEUCAS ASPERA USING IN VITRO MODELS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i11.13711 ◽

2016 ◽

Vol 8 (12) ◽

pp. 292 ◽

Cited By ~ 3

Author(s):

Tahareen S. ◽

Shwetha R. ◽

Myrene R. D.

Keyword(s):

Inflammatory Activity ◽

Total Phenolic ◽

Scavenging Activity ◽

Membrane Stabilization ◽

Standard Drug ◽

Leucas Aspera ◽

Ic50 Value ◽

Anti Inflammatory ◽

Stabilization Assay

Objective: To evaluate the potential antioxidant, anti-inflammatory and antibacterial activities of aqueous and methanolic extracts of leaves of Leucas aspera (Thumbae).Methods: Phytochemical screening of the leaves of L. aspera was followed by analysis of antioxidant activity by means of DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity. In vitro anti‐inflammatory activity was evaluated using lipoxygenase inhibition, albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of anti‐inflammatory activity. Linear regression analysis was used to calculate half maximal inhibitory concentration, IC50 value. The zone of inhibition was performed against common pathogens to determine the antimicrobial activity at different concentrations of plant extracts (60%, 70%, 80%).Results: The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, tannins, flavonoids, glycosides, xanthoproteins, and phenols. The total phenolic and flavonoid content was found to be 2.25±0.04 mg GAE/g (gallic acid equivalents) and 1.2±0.05 mg QE/g (Quercetin equivalents) of fresh weight tissue respectively. The IC50 values for hydrogen peroxide scavenging activity were found to be 244.6 µg/ml. The extract inhibited the lipoxygenase enzyme activity with an IC50 value of 356.3 µg/ml. Maximum inhibition of heat-induced protein denaturation of 69% was observed at 400 μg/ml, IC50 249.6 μg/ml. Proteinase activity was also significantly inhibited (IC50 = 421.6 μg/ml). Membrane stabilization assay attributed minor protection by the leaf extract with an IC50 of 206.7. It was observed that E. coli were inhibited at all concentrations, followed by Klebsiella and Pseudomonas.Conclusion: Results indicate that L. aspera possess anti-inflammatory properties due to the strong occurrence of polyphenolic compounds such as alkaloids, flavonoids, tannins and steroids that serve as free radical inhibitors or scavenger. Compounds of the plant L. aspera may hence be used as lead compounds for designing potent anti-inflammatory drug which can be used for treatment of various diseases.

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In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

Brazilian Journal of Biological Sciences ◽

10.21472/bjbs.051018 ◽

2018 ◽

Vol 5 (10) ◽

pp. 417-426 ◽

Cited By ~ 1

Author(s):

Samanjit Kaur ◽

M. Syed Ali ◽

V. Anuradha ◽

V. Suganya ◽

A. Ashashalini ◽

...

Keyword(s):

Protein Denaturation ◽

Inflammatory Activity ◽

Bark Extract ◽

Root Extract ◽

Rhizophora Mucronata ◽

Membrane Stabilization ◽

In Vivo Models ◽

Maximum Inhibition ◽

Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

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PHYTOCHEMICAL ANALYSIS AND SUPPRESSION OF INFLAMMATORY TARGETS BY ADATHODA VASICA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i5.24243 ◽

2018 ◽

Vol 11 (5) ◽

pp. 162

Author(s):

Amala Reddy ◽

Sujatha Sundaresan

Keyword(s):

Methanolic Extract ◽

Maximum Yield ◽

Foot Ulcer ◽

Significant Inhibition ◽

Inflammatory Activity ◽

Phytochemical Analysis ◽

Membrane Stabilization ◽

Standard Drug ◽

Anti Inflammatory

Objectives: A persistent and chronic inflammation may act as an underlying definition for many of the long-term illness such as diabetic foot ulcer, Alzheimer’s disease and rheumatoid arthritis. Folklore medicinal plants provide immense health benefits with new remedies. Active compounds from plants containing alkaloids and phenolic compounds (flavonoids and tannins) possess antioxidant and anti-inflammatory activity. The leaves of Adhatoda vasica are most known for its effectiveness in treating respiratory diseases such as asthma, chronic bronchitis, and other respiratory conditions. In this report, we investigated the possible action of A. vasica against inflammation.Methods: Solvents with varying polarity (Hexane, dichloromethane, ethyl acetate, and methanol) were used for the sequential extraction of leaves of A. vasica Linn (Family: Acanthaceae). The anti‐inflammatory activity was assessed using simple in vitro methods. Phenols, flavonoids, phytosterols, saponins, alkaloids, and tannins were analyzed qualitatively using standard protocols. Anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity. Aspirin (diclofenac) was used as a standard drug for the study of anti-inflammatory activity.Results: Our results substantiated that methanolic extract of A. vasica gave a maximum yield of 12.5% and apparently all bioactive phytochemical constituents were at a higher concentration in the methanolic extract. Methanolic extract of A. vasica showed a significant inhibition of the heat-induced albumin denaturation, red blood cells membrane stabilization, and proteinase inhibitory effects with 86±1.46, 88±1.24, and 96±0.93% for 1000 μg/ml, respectively.Conclusion: The above results delineate that the bioactive components were extracted using high polar solvent are mostly flavonoids and related polyphenols. These compounds may be the active constituents to render the anti-inflammatory activity to A. vasica.

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STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.28479 ◽

2019 ◽

Vol 12 (1) ◽

pp. 95 ◽

Cited By ~ 1

Author(s):

Yogesh Diwakar ◽

Chitra V ◽

Evelyn Sharon S

Keyword(s):

Protein Denaturation ◽

Inflammatory Activity ◽

Maximum Effect ◽

Dependent Manner ◽

Membrane Stabilization ◽

Human Red Blood Cells ◽

Phytochemical Compounds ◽

Anti Inflammatory ◽

Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

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Assessment of In-vitro Anti-Inflammatory Activity of Cynodon Dactylon and Acyclovir Showing Synergistic Effect by Albumin Denaturation and Membrane Stabilization Assay

Modern Approaches in Drug Designing ◽

10.31031/madd.2017.01.000506 ◽

2017 ◽

Vol 1 (2) ◽

Cited By ~ 3

Author(s):

Suchita V Ghumre

Keyword(s):

Synergistic Effect ◽

Cynodon Dactylon ◽

Inflammatory Activity ◽

Membrane Stabilization ◽

Anti Inflammatory ◽

Stabilization Assay

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Inhibitory effects of hydroethanolic extracts from three Cameroonian medicinal plants on proteins inflammation and growth of multi-resistant strains of Mycobacterium tuberculosis

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i4-s.4930 ◽

2021 ◽

Vol 11 (4-S) ◽

pp. 15-21

Author(s):

Esther Del florence Ndedi Moni ◽

Patrick Hervé Diboue Betote ◽

Christelle Wayoue Kom ◽

Chimène Félicite Mekoulou Benga ◽

Armelle Deutou Tchamgoue ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Medicinal Plants ◽

Inhibitory Action ◽

Ethanol Extract ◽

Inflammatory Activity ◽

Phytochemical Screening ◽

Resistant Strains ◽

Anti Inflammatory ◽

Ethanol Extracts

The present work aimed to determine the phytochemical components and evaluate the in vitro anti-inflammatory and anti-mycobacterial effects of hydroethanolic extracts of Allium sativum L bulbs, Drypetes gossweileri S. MOORE stem-barks and Pentadiplandra brazzeana Baill roots against several resistant strains of Mycobacterium tuberculosis. The phytochemical screenings of extracts were carried out according the colorimetric and precipitation tests to reveal the presence of phytochemical compounds. The anti-inflammatory effects of extracts were evaluated using in vitro Bovine Serum Albumin denaturation and proteinase inhibitory action assays. The inhibitory parameters of hydro-ethanol extracts were evaluated by the microdilution method agaisnt Mycobacterium tuberculosis. The phytochemical screening of hydro-ethanol extracts revealed the presence of phenols, polyphenols, flavonoids, alkaloids, cathechic tannins, triterpens, steroids, anthocyanins and leucoanthocyanins. The anti-inflammatory activity of hydro-ethanol extracts of D. gossweileri, P. brazzeana and A. sativum have shown the inhibitory concentrations 50 (IC50) values ranging from 356.70, 183.30 and 226.30 mg/mL for BSA denaturation and 31.92, 33.62 and 56.93 mg/mL for proteinase inhibitory action respectively. The hydroethanolic extracts of D. gossweileri, P. brazzeana and A. sativum exhibited moderate and weak anti-mycobacterial activities with the minimum inhibitory concentrations (MICs) ranging from 312.5 to 2500 μg/mL. A. sativum hydro-ethanol extract has shown the highest anti-mycobacterial activity with MIC of 312.5 μg/mL against isoniazid resistant of M. tuberculosis and extremely resistant drug strain of M. tuberculosis. These results suggest that hydro-ethanol extracts of A. sativum, D. gossweileri and P. brazzeana are efficient against tuberculosis caused by multi-resistant Mycobacterium tuberculosis strains and are able to resorb the inflammation induced during infection. Keywords: Anti-inflammatory activity, Anti-mycobacterial effect, Hydroethanolic extracts, Medicinal plants, Phytochemical screening.

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