Effect of Ornamental Stone Waste Incorporation on the Rheology, Hydration, Microstructure, and CO2 Emissions of Ordinary Portland Cement

The ornamental stone industry generates large amounts of waste thus creating environmental and human health hazards. Thus, pastes with 0–30 wt.% ornamental stone waste (OSW) incorporated into ordinary Portland cement (OPC) were produced and their rheological properties, hydration kinetics, and mechanical properties were evaluated. The CO2 equivalent emissions related to the pastes production were estimated for each composition. The results showed that the paste with 10 wt.% of OSW exhibited similar yield stress compared to the plain OPC paste, while pastes with 20 and 30 wt.% displayed reduced yield stresses up to 15%. OSW slightly enhanced the hydration kinetics compared to plain OPC, increasing the main heat flow peak and 90-h cumulative heat values. The incorporation of OSW reduced the 1-, 3-, and 28-days compressive strength of the pastes. Water absorption results agreed with the 28 days compressive strength results, indicating that OSW increased the volume of permeable voids. Finally, OSW incorporation progressively reduced the CO2 emission per m3 of OPC paste, reaching a 31% reduction for the highest 30 wt.% OSW content. Overall, incorporating up to 10 wt.% with OSW led to pastes with comparable fresh and hardened properties as comported to plain OPC paste.

Extraction of Polyhydroxyalkanoates from Purple Non-Sulfur Bacteria by Non-Chlorinated Solvents

In this study, non-chlorinated solvents such as cyclohexanone (CYC) and three ionic liquids, (ILs) (1-ethyl-3-methylimidazolium dimethylphosphate, [EMIM][DMP], 1-ethyl-3-methylimidazolium diethylphosphate, [EMIM][DEP] and 1-ethyl-3-methylimidazolium methylphosphite, [EMIM][MP]) were tested to extract polyhydroxyalkanoates (PHAs) from the purple non-sulfur photosynthetic bacterium (PNSB) Rhodovulumsulfidophilum DSM-1374. The photosynthetic bacterium was cultured in a new generation photobioreactor with 4 L of working volume using a lactate-rich medium. The extracted PHAs were characterized using a thermogravimetric analysis, differential scanning calorimetry, infrared spectroscopy, proton nuclear magnetic resonance and gel permeation chromatography. The most promising results were obtained with CYC at 125 °C with an extraction time of above 10 min, obtaining extraction yields higher than 95% and a highly pure poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with around 2.7 mol% of hydroxylvalerate (HV). A similar yield and purity were obtained with chloroform (CHL) at 10 °C for 24 h, which was used as the referent solvent Although the three investigated ILs at 60 °C for 4 and 24 h with biomass/IL up to 1/30 (w/w) obtained PHAs strongly contaminated by cellular membrane residues, they were not completely solubilized by the investigated ILs.

Dual-site blood culture yield and time to positivity in neonatal late-onset sepsis

ObjectiveTo determine whether culture yield and time to positivity (TTP) differ between peripheral and central vascular catheter-derived blood cultures (BCx) in neonatal intensive care unit (NICU) patients evaluated for late-onset sepsis.DesignSingle-centre, retrospective, observational study.SettingLevel IV NICU.ParticipantsThe study included infants >72 hours old admitted to NICU in 2007–2019 with culture-confirmed bacteraemia. All episodes had simultaneous BCx drawn from a peripheral site and a vascular catheter (‘catheter culture’).Main outcome measuresDual-site culture yield and TTP.ResultsAmong 179 episodes of late-onset bacteraemia (among 167 infants) with concurrently drawn peripheral and catheter BCx, the majority (67%, 120 of 179) were positive from both sites, compared with 17% (30 of 179) with positive catheter cultures only and 16% (29 of 179) with positive peripheral cultures only. 66% (19 of 29) of episodes with only positive peripheral BCx grew coagulase-negative Staphylococcus, while 34% (10 of 29) were recognised bacterial pathogens. Among 120 episodes with both peripheral and catheter BCx growth, catheter cultures demonstrated bacterial growth prior to paired peripheral cultures in 78% of episodes (93 of 120, p<0.001). The median TTP was significantly shorter in catheter compared with peripheral cultures (15.0 hours vs 16.8 hours, p<0.001). The median elapsed time between paired catheter and peripheral culture growth was 1.3 hours.ConclusionConcurrently drawn peripheral and catheter BCx had similar yield. While a majority of episodes demonstrated dual-site BCx growth, a small but important minority of episodes grew virulent pathogens from either culture site alone. While dual-site culture practices may be useful, clinicians should balance the gain in sensitivity of bacteraemia detection against additive contamination risk.

‘Atlantic’ and ‘Dakota Pearl’ Chipping Potato Responses to Glyphosate and Dicamba Simulated Drift

Field trials were conducted to determine the effects of glyphosate and/or dicamba simulated drift rates on chipping potatoes ‘Atlantic’ and ‘Dakota Pearl’. Sublethal herbicide rates were applied at the tuber initiation stage and consisted of dicamba at 99 g ae ha−1 or glyphosate at 197 g ae ha−1 applied alone or the combinations of dicamba at 20 or 99 g ae ha−1 and glyphosate at 40 or 197 g ae ha−1, respectively. At 7 days after treatment (DAT), the high spray combination of glyphosate plus dicamba resulted in the greatest plant damage (28%). Plant injury from plants treated with the low combination of glyphosate plus dicamba did not differ from the nontreated control. At 21 DAT, visible injury increased to 40% for plants treated with the high combination of glyphosate plus dicamba treatment. Total yield suggested that dicamba and glyphosate caused similar yield reductions as plants that received glyphosate at 197 g ha−1 or dicamba at 99 g ha−1 had lower total yields compared to the nontreated and plants that received the combination of glyphosate (197 g ha−1) and dicamba (99 g ha−1) had lower total yields compared to plants that received either herbicide alone. However, ‘Dakota Pearl’ plants were more sensitive to glyphosate at 197 g ha−1 than ‘Atlantic’ causing the interaction for most tuber grades. Tuber specific gravity was lower for plants that received glyphosate at 197 g ha−1, dicamba at 99 g ha−1, or this combination, but this reduction would not prevent chip processing. Results reinforce the need for diligence when applying these herbicides in proximity to a susceptible crop such as chipping potatoes and the need to thoroughly clean sprayers before applications to a sensitive crop.

Utilizing Cover Crops for Weed Suppression within Buffer Areas of 2,4-D-Resistant Soybean

Cover crops can be utilized to suppress weeds via direct competition for sunlight, water, and soil nutrients. Research was conducted to determine if cover crops can be used in label mandated buffer areas in 2,4-D-resistant soybean cropping systems. Delaying termination of cover crops containing cereal rye to at, or after, soybean planting resulted in a 25 to over 200 percentage point increase in cover crop biomass compared to a control treatment. Cover crops generally improved horseweed control when 2,4-D was not used. Cover crops reduced grass densities up to 54% at four of six site-years when termination was delayed to after soybean planting. Cover crops did not reduce giant ragweed densities. Cover crops reduced waterhemp densities by up to 45%. Cover crops terminated at, or after planting were beneficial within buffer areas for control of grassess and waterhemp, but not giant ragweed. Yield reductions of 14 to 41% occurred when cover crop termination was delayed to after soybean planting at three of six site-years. Terminating the cover crops at planting time provided suppression of grasses and waterhemp within buffer areas and had similar yield to the highest yielding treatment in five out of six site-years.

Influence of Recovery Treatments on Dicamba-injured Soybean

Non-dicamba resistant soybean yield loss resulting from dicamba off-target injury has become an increasing concern for soybean growers in recent years. After off-target dicamba movement occurs onto sensitive soybean, little information is available on tactics that could be used to mitigate the cosmetic or yield losses that may occur. Therefore, a field experiment was conducted in 2017, 2018, and 2019 to determine if certain recovery treatments of fungicide, plant growth hormone, macro- and micronutrient fertilizer combinations, or weekly irrigation could reduce dicamba injury and/or result in similar yield to soybean that was not injured with dicamba. Simulated drift events of dicamba (5.6 g ae ha−1) were applied to non-dicamba resistant soybean once they reached the V3 or R2 stages of growth. Recovery treatments were applied approximately 14 d after the simulated drift event. Weekly irrigation was the only recovery treatment that provided appreciable levels of injury reduction or increases in soybean height or yield compared to the dicamba-injured plants. Weekly irrigation following the R2 dicamba injury event resulted in an 1% to 14% increase in soybean yield compared to the dicamba-injured control. All other recovery treatments resulted in soybean yields similar to the dicamba-injured control, and similar to or lower than the non-treated control. Results from this study indicate that if soybean have become injured with dicamba, weekly irrigation will help soybean recover some of the yield loss and reduce injury symptoms that resulted from off-target dicamba movement, especially in a year with below average precipitation. However, yield loss will likely not be restored to that of non-injured soybean.

Hydrogen diffusion and trapping in laser additively manufactured ultra-high strength AerMet100 steel

Hydrogen trapping and the permeation behavior of laser additively manufactured (LAM) AerMet100 steel with an as-deposited specimen (AD) and after three types of heat-treated specimens (BM, TBMM, and TM) was investigated. At least three types of different hydrogen traps were identified in each microstructure of LAM AerMet100 steel, including both reversible and irreversible H traps. For as-deposited microstructure, the main reversible H trap states are related to the precipitation of M3C carbides associated with a detrapping activation energy (Ed) of 17.3±0.2 kJ/mol. After heat treatment, the dominant reversible hydrogen trap states in the tempered martensite microstructure have a different Ed value of 19.3±0.5 kJ/mol, which is attributed to the precipitation of highly coherent M2C carbides. In comparison with the reported Ed value of ~21.4 kJ/mol for main reversible hydrogen traps in wrought AerMet100 steel, the less Ed value in LAM AerMet100 steel is closely related to the composition change of M2C carbides. In all of the H pre-charged samples, the diffusible and total H concentration of the TM specimen and the TBMM specimen are about 3-4 times higher than that of the AD specimen and the BM specimen. The TM specimen with tempered martensite microstructure has the highest diffusible and total H concentration due to its high density of dominantly reversible H traps. The effective hydrogen diffusion coefficient (Deff) of LAM AerMet100 steel is on the order of 10-9 cm2/s, and decreases with increasing density of dominantly reversible H traps brought about by heat treatment. Furthermore, compared with wrought AerMet100 steel of a similar yield strength (~1750 MPa), the LAM AerMet100 steel has a comparable Deff of about 2.8×10-9 cm2/s.

Photodissociation Dynamics of the Cyclohexyl Radical from the 3p Rydberg State at 248 nm

The photodissociation of jet-cooled cyclohexyl was studied by exciting the radicals to their 3p Rydberg state using 248 nm laser light and detecting photoproducts by photofragment translational spectroscopy. Both H-atom loss and dissociation to heavy fragment pairs are observed. The H-atom loss channel exhibits a two-component translational energy distribution. The fast photoproduct component is attributed to impulsive cleavage directly from an excited state, likely the Rydberg 3s state, forming cyclohexene. The slow component is due to statistical decomposition of hot cyclohexyl radicals that internally convert to the ground electronic state prior to H-atom loss. The fast and slow components are present in a ~0.7:1 ratio, similar to findings in other alkyl radicals. Internal conversion to the ground state also leads to ring-opening followed by dissociation to 1-buten-4-yl + ethene in comparable yield to H-loss, with the C₄H₇ fragment containing enough internal energy to dissociate further to butadiene via H-atom loss. A very minor ground-state C₅H₈ + CH₃ channel is observed, attributed predominantly to 1,3-pentadiene formation. The ground-state branching ratios agree well with RRKM calculations, which also predict C₄H₆ + C₂H₅ and C₃H₆ + C₃H₅ channels with similar yield to C₅H₈ + CH₃. If these channels were active it was at levels too low to be observed.

Photodissociation Dynamics of the Cyclohexyl Radical from the 3p Rydberg State at 248 nm

The photodissociation of jet-cooled cyclohexyl was studied by exciting the radicals to their 3p Rydberg state using 248 nm laser light and detecting photoproducts by photofragment translational spectroscopy. Both H-atom loss and dissociation to heavy fragment pairs are observed. The H-atom loss channel exhibits a two-component translational energy distribution. The fast photoproduct component is attributed to impulsive cleavage directly from an excited state, likely the Rydberg 3s state, forming cyclohexene. The slow component is due to statistical decomposition of hot cyclohexyl radicals that internally convert to the ground electronic state prior to H-atom loss. The fast and slow components are present in a ~0.7:1 ratio, similar to findings in other alkyl radicals. Internal conversion to the ground state also leads to ring-opening followed by dissociation to 1-buten-4-yl + ethene in comparable yield to H-loss, with the C₄H₇ fragment containing enough internal energy to dissociate further to butadiene via H-atom loss. A very minor ground-state C₅H₈ + CH₃ channel is observed, attributed predominantly to 1,3-pentadiene formation. The ground-state branching ratios agree well with RRKM calculations, which also predict C₄H₆ + C₂H₅ and C₃H₆ + C₃H₅ channels with similar yield to C₅H₈ + CH₃. If these channels were active it was at levels too low to be observed.

Performance of rubber seed oil as an alternative to diesel in oilbased drilling mud formulation

The formulation of an oil-based mud was made possible with the oil extracted from rubber seeds using the famous soxhlet extraction method. The mud was formulated using the API standard of 25 g of bentonite to 350 mL base fluid. The choice of Rubber oil comes as a result of its flash point and aniline point which lies in the range of base oils used for mud formulation. The rheological properties of the rubber OBM were beyond the scope of the viscometer and hence thinner was added to reduce its viscosity and its suitability to compete favorably with diesel OBM was checked. The 10-sec and 10-min gel strength of the Rubber OBM was recorded as 68 lb/100 ft2 and 69 lb/100 ft2 respectively while that of Diesel was 65 lb/100 ft2 and 67 LB/100 ft2 . The plastic viscosity of Rubber OBM was 12 cp while that of Diesel was 17 cp. They both exhibited Bingham Plastic behavior and a similar yield point of 146 lb/100 ft2 . The formulated mud samples were subjected to temperatures of 60 oC and 75 oC and it was discovered that Rubber OBM was likely to retain its rheological property than diesel OBM. Comparison with other rheological properties of diesel OBM showed that the formulated mud could be used alternatively for diesel in drilling operations.