scholarly journals Haematology of Birds and Microbial Analysis of Recycled Poultry Litter Treated with Graded Levels of Aluminium Sulphate (Alum)

2021 ◽  
pp. 23-30
Author(s):  
A. A. Usman ◽  
T. S. Olugbemi ◽  
J. J. Omage ◽  
H. B. Usman
Keyword(s):  
Poultry Litter ◽  
Clinical Pathology ◽  
Microbial Load ◽  
Control Group ◽  
Salmonella Spp ◽  
Aluminium Sulphate ◽  
Pathology Laboratory ◽  
Wing Vein ◽  
Four Corners ◽  
Microbial Analysis

The study was carried out at the poultry unit of the Department of Animal Science teaching and research farm, Ahmadu Bello University, Zaria to determine the Chemical and Microbial Analysis of Poultry Litter Treated with Graded Levels of Aluminium sulphate (Alum). The alum used was obtained from the Sabon-garimarket in Zaria, Kaduna State. Aluminium sulphate (alum) was applied to the wood shavings by mixing it with alum thoroughly using hands covered with hand gloves. The rates of alum application was as follows: T1 control (normal poultry litter with no alum), T2 (5% alum by kg weight of poultry litter), T3 (10% alum by kg weight of poultry litter) and T4 (15% alum by kg weight of poultry litter). Five sets of litter samples were obtained fortnightly from each pen from different locations i.e. the four corners and centre from which the microbial load were measured. At the termination of the experiment (day 56), two birds from each pen having representative weights for the group (6 birds per Treatment) were selected and 1.5ml of blood was taken via the wing vein. Haematological parameters were analyzed by an auto haemo-analyser (BC2800 vet auto haemo analyser) at the Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria. This result shows significant (P<0.05) difference among all the parameters analysed except MCHC. The result shows a significant decrease in the total bacteria, E. coliand Salmonella spp. in the alum treated litter groups (5%, 10% and 15% alum treated litter) compared to the control group (0% alum treated litter), the mould and yeast load was significantly increased in alum treated litter groups (5%, 10% and 15% alum treated litter) compared to the control group (0% alum treated litter).The study conclude that treating recycled poultry litter with alum can reduce microbial load of the litter. Birds reared on recycled poultry litter have significantly higher haemoglobin and PCV compared to the control.

ANGKA BANDING ALBUMIN KREATININ AIR KEMIH DAN HbA1C SERTA ESTIMASI LAJU FILTRASI GLOMERULUS PADA PASIEN DIABETES MELITUS TIPE 2

2016 ◽  
Vol 21 (3) ◽  
pp. 250
Author(s):  
Amiroh Kurniati ◽  
Tahono Tahono
Keyword(s):  
Glycemic Control ◽  
Clinical Pathology ◽  
Poor Glycemic Control ◽  
Control Group ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Chronic Hyperglycemia ◽  
Type 2 Dm ◽  
Spearman Correlation Test

Diabetes Mellitus (DM) type 2 is a metabolic disease that prevalence increasing. A chronic hyperglycemia with poor glycemic control can stimulate oxidative stress, which will continue to occurrence of complications in the kidneys characterized by the presenceof microalbuminuria can be measured by the ratio of urinary albumin creatinine ratio (UACR) and the change in estimated glomerular filtration rate (eGFR). The aims of this study was to know the correlation between the UACR with HbA1c value and eGFR in patients with type 2 DM by finding them out. This study used cross sectional research design. Subjects were patients with type 2 DM who attend control in Endocrinology Subdivision of Internal Medicine Departement and perform blood and urine tests in Clinical Pathology Laboratory in Dr. Moewardi Hospital Surakarta in August 2013. To determine the pattern of the data distribution, the researchers used KolmogorovSmirnov test, and to analyse the result used Spearman (r) correlation with p<0.05 and confidence interval 95%. Statistical analysis using Spearman correlation test (r), significant when p<0.05 with 95% confidence intervals. From 68 samples examined the mean age is 60.9 year old, with equal participants for male and female (34 subjects each). Most subjects were in poor glycemic control group (72.1%) and in the range of microalbuminuria (44.1%). There was a significant correlation between UACR with HbA1c and eGFR in type 2 DM patient (r=0.412, p=0.000; and r= -0.270, p=0.02, respectively). Based on this study it can be concluded that increased UACR were associated with worsened glycemic control and were characterized by higher levels of HbA1c and its eGFR value would be lower. Further analysis requires further research with a larger sample size and more attention to the factors that may affect the related examination

CD40L-CD40 interaction avidity as coronary artery disease predictive factor

2021 ◽  
Vol 20 (4) ◽  
pp. 796-800
Author(s):  
Linda Rosita ◽  
Erlina Marfianti ◽  
Ninda Devita ◽  
Adika Zhulhi Arjana
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Coronary Arteries ◽  
Medical Science ◽  
Clinical Pathology ◽  
Control Group ◽  
Pathology Laboratory ◽  
Healthy Control ◽  
Cd40 Expression ◽  
Artery Disease

Background: Coronary artery disease (CAD) can occur due to atherosclerosis in coronary arteries. Platelet agreement plays an important role in the pathophysiology of CAD. CD40L is a surface antigen on activated platelets. CD40L will bind to CD40 which is expressed by macrophages and endothelials then this activation results in reduced thickness and stability of atherosclerotic plaque and then thrombus appears. Thrombus ultimately inhibits blood flow to the coronary arteries. Proper measurement of these activities can describe the occurrence of CADs driven by platelets. Objectives: Measuring the avidity of CD40L-CD40 interactions so that the relationship between CD40L activity and CAD events can be seen. Materials and Methods: This research is non-experimental in nature and uses Case control designs. The subjects of the study were CAD patients at RSUD Dr. Soedirman Kebumen. Patients who met the inclusion and exclusion criteria were then examined and healthy patients were matched and CAD patients matched. The subject’s blood is then drawn and sent to Clinical Pathology Laboratory, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada. Blood samples were then examined using flow cytometry to determine CD40L levels. The results obtained were then analyzed by logistic regression test with Medcalc software. Results and discussion: Twenty-six subjects participated in this study, with 13 subjects were CAD patients and 13 healthy control subjects.The percentage of platelets expressing CD62P+ CD40L+ in the CAD group was higher than in the control group (p = 0.0015). Statistical analysis with T test showed that there were significant differences in CD40-expressing platelets between the CAD group and the control group (p = 0.0029).The study conclude that the avidity of CD40L-CD40 interactions as indicated by CD40 expression is related to CAD events. CD40 expression was higher in subjects with CAD compared to controls. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.796-800

scholarly journals Chemical and Microbial Analysis of Bedding Material (Wood Shavings) Treated with Graded Levels of Aluminium Sulphate (Alum)

2021 ◽  
pp. 32-41
Author(s):  
A. A. Usman ◽  
T. S. Olugbemi ◽  
J. J. Omage ◽  
K. M. Aljameel
Keyword(s):  
Ph Value ◽  
Soluble Reactive Phosphorus ◽  
Treated Wood ◽  
Untreated Wood ◽  
Microbial Load ◽  
Normal Wood ◽  
Salmonella Spp ◽  
Wood Shavings ◽  
Microbial Analysis ◽  
Reactive Phosphorus

The study was carried out at the poultry unit of the Department of Animal Science teaching and research farm, Ahmadu Bello University, Zaria to determine the chemical and microbial analysis of poultry litter (wood shavings) treated with graded levels of alum. The alum used was obtained from the Sabon-gari market in Zaria, Kaduna State. The rates of alum application (prior to keeping the birds) was as follows: T1 control (normal wood shavings with no alum), T2 (5% alum by kg weight of wood shavings), T3 (10% alum by kg weight of wood shavings) and T4 (15% alum by kg weight of wood shavings). Five sets of litter samples were obtained fortnightly from each pen from different locations i.e. the four corners and center from which the microbial load, pH, total nitrogen (N), soluble reactive phosphorus, VFA and NH4+ concentration were measured. The result shows significantly (P<0.05) lower pH value in all the alum treated wood shavings groups (5%, 10% and 15% alum treated wood shavings) compared to the control. The result showed that significantly (P<0.05) lower total volatile fatty acid level was obtained in all the alum treated wood shavings groups (5%, 10% and 15% alum treated wood shavings) compared to the control untreated wood shaving group. The results showed a decrease in total bacteria, E. coli and Salmonella spp. load in alum treated wood shavings groups (5%, 10% and 15% alum treated wood shavings) compared to the control, while mould and yeast load was increased in all the alum treated wood shavings groups (5%, 10% and 15% alum treated wood shavings) compared to the control. The study conclude that treating wood shavings with alum can reduce microbial load of the litter, hence improve health and reduce mortality. Treating wood shavings with alum tends reduce the microbial load of the litter.

scholarly journals ANGKA BANDING ALBUMIN KREATININ AIR KEMIH DAN HbA1C SERTA ESTIMASI LAJU FILTRASI GLOMERULUS PADA PASIEN DIABETES MELITUS TIPE 2 (Urinary Albumin to Creatinine Ratio With HbA1c and Estimated Glomerulo Filtration Rate in Type 2 Diabetes Mellitus Patients)

2018 ◽  
Vol 21 (3) ◽  
pp. 250
Author(s):  
Amiroh Kurniati ◽  
Tahono Tahono
Keyword(s):  
Diabetes Mellitus ◽  
Glycemic Control ◽  
Clinical Pathology ◽  
Urinary Albumin ◽  
Control Group ◽  
Creatinine Ratio ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Chronic Hyperglycemia

Diabetes Mellitus (DM) type 2 is a metabolic disease that prevalence increasing. A chronic hyperglycemia with poor glycemiccontrol can stimulate oxidative stress, which will continue to occurrence of complications in the kidneys characterized by the presenceof microalbuminuria can be measured by the ratio of urinary albumin creatinine ratio (UACR) and the change in estimated glomerularfiltration rate (eGFR). The aims of this study was to know the correlation between the UACR with HbA1c value and eGFR in patients withtype 2 DM by finding them out. This study used cross sectional research design. Subjects were patients with type 2 DM who attend controlin Endocrinology Subdivision of Internal Medicine Departement and perform blood and urine tests in Clinical Pathology Laboratory inDr. Moewardi Hospital Surakarta in August 2013. To determine the pattern of the data distribution, the researchers used KolmogorovSmirnov test, and to analyse the result used Spearman (r) correlation with p<0.05 and confidence interval 95%. Statistical analysisusing Spearman correlation test (r), significant when p<0.05 with 95% confidence intervals. From 68 samples examined the meanage is 60.9 year old, with equal participants for male and female (34 subjects each). Most subjects were in poor glycemic control group(72.1%) and in the range of microalbuminuria (44.1%). There was a significant correlation between UACR with HbA1c and eGFR intype 2 DM patient (r=0.412, p=0.000; and r= -0.270, p=0.02, respectively). Based on this study it can be concluded that increasedUACR were associated with worsened glycemic control and were characterized by higher levels of HbA1c and its eGFR value would belower. Further analysis requires further research with a larger sample size and more attention to the factors that may affect the relatedexamination.

scholarly journals Indispensability of Clinical Bioinformatics for Effective Implementation of Genomic Medicine in Pathology Laboratories

ACI Open ◽  
2020 ◽  
Vol 04 (02) ◽  
pp. e167-e172
Author(s):  
Srikar Chamala ◽  
Siddardha Majety ◽  
Shesh Nath Mishra ◽  
Kimberly J. Newsom ◽  
Shaileshbhai Revabhai Gothi ◽  
...  
Keyword(s):  
Patient Care ◽  
Clinical Laboratory ◽  
Clinical Care ◽  
Genomic Medicine ◽  
Clinical Pathology ◽  
Genetic Mutation ◽  
Genomic Research ◽  
Pathology Laboratory ◽  
Clinical Bioinformatics ◽  
Effective Implementation

AbstractPatient care is rapidly evolving toward the inclusion of precision genomic medicine when genomic tests are used by clinicians to determine disease predisposition, prognosis, diagnosis, and improve therapeutic decision-making. However, unlike other clinical pathology laboratory tests, the development, deployment, and delivery of genomic tests and results are an intricate process. Genomic technologies are diverse, fast changing, and generate massive data. Implementation of these technologies in a Clinical Laboratory Improvement Amendments-certified and College of American Pathologists-accredited pathology laboratory often require custom clinical grade computational data analysis and management workflows. Additionally, accurate classification and reporting of clinically actionable genetic mutation requires well-curated disease/application-specific knowledgebases and expertise. Moreover, lack of “out of the box” technical features in electronic health record systems necessitates custom solutions for communicating genetic information to clinicians and patients. Genomic data generated as part of clinical care easily adds great value for translational research. In this article, we discuss current and future innovative clinical bioinformatics solutions and workflows developed at our institution for effective implementation of precision genomic medicine across molecular pathology, patient care, and translational genomic research.

Use of cost-effective technologies for a routine clinical pathology laboratory

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Mercedes Vazquez ◽  
L. Anfossi ◽  
Hader Ben-Yoav ◽  
Lorena Diéguez ◽  
Thomas Karopka ◽  
...  
Keyword(s):  
Developing Countries ◽  
Clinical Pathology ◽  
Cost Effective ◽  
Limiting Factor ◽  
Economic Costs ◽  
Pathology Laboratory

Classically, the need for highly sophisticated instruments with important economic costs has been a major limiting factor for clinical pathology laboratories, especially in developing countries. With the aim of making...

scholarly journals Molasses as a possible cause of an ''endocrine disruptive syndrome'' in calves

2009 ◽  
Vol 76 (2) ◽  
Author(s):  
M.S. Masgoret ◽  
C.J. Botha ◽  
J.G. Myburgh ◽  
T.W. Naude ◽  
L. Prozesky ◽  
...  
Keyword(s):  
Clinical Pathology ◽  
Mass Gain ◽  
In Vitro Assays ◽  
Control Group ◽  
Dairy Herds ◽  
Disruptive Effect ◽  
Feeding Trial ◽  
Cattle Feed ◽  
Bull Calves

During the mid 1990s a potentially serious, chronic syndrome was reported in well-managed beef and dairy herds from unrelated parts of South Africa. Farmers reported that it manifested as various combinations of decreased production, decreased weaning masses, apparent immune breakdown in previously immunocompetent animals, increased reproductive disorders, various mineral imbalances in non-deficient areas and goitre, noticeable as enlarged thyroid glands. The farmers associated this syndrome with certain batches of sugar cane molasses and molasses-based products. The syndrome was reminiscent of an ''endocrine disruptive syndrome''. The objective of this study was to evaluate the suspected endocrine disruptive effect of molasses included in cattle feed. Using existing in vitro assays, four batches of molasses syrup were screened for possible inclusion in a calf feeding trial. Two batches were selected for the trial. Thirty-two, 4- to 6-week-old, weaned Holstein bull calves were included in the single phase, three treatment, parallel design experiment. In two of the groups of calves, two different batches of molasses were included in their rations respectively. The control group was fed a ration to which no molasses was added, but which was balanced for energy and mineral content. The mass gain of the calves was recorded over the 6-month study period. The calves were clinically examined every week and clinical pathology parameters, immune responses and endocrine effects were regularly evaluated. Even though endocrine disrupting effects were detected with the in vitro screening assays, these could not be reproduced in the calves in the experiment. The two batches of molasses utilized in the calf feeding trial did not induce major differences in any of the parameters measured, with the exception of a lower mass gain in one of the molasses-fed groups (Group 1), which tended towards significance. The results of the study indicate that the two batches of molasses had no endocrine disruptive or immunosuppressive effects in calves.

scholarly journals ANALISIS BEBAN KERJA DI INSTALASI LABORATORIUM

2016 ◽  
Vol 20 (1) ◽  
pp. 64
Author(s):  
Amiroh Kurniati ◽  
Tahono Tahono
Keyword(s):  
Clinical Pathology ◽  
Work Load ◽  
Accurate Method ◽  
Development Planning ◽  
Smear Microscopy ◽  
Pathology Laboratory ◽  
Laboratory Installation ◽  
Laboratory Service ◽  
Laboratory Staff ◽  
Manpower Needs

Human resources (HR) planning is associated with an institution’s strategic plan, so the study of the required number of support personnel is in line with the direction of business development planning of the Laboratory Installation Unit. WISN (work load indicator staff need) method is a calculation based on the analysis of health manpower needs of the workload (work load analysis) and is considered as the most accurate method to calculate labour requirements in short-term. Based on the results of the analysis of manpower needs at the Laboratory Installation of Lung Health of the Community Hall Surakarta, it is known that there is still a lack of a number of five (5) persons of the health laboratory staff. The persons still lacking consist of: one (1) laboratory person related to clinical pathology laboratory, one (1) laboratory person for smear microscopy laboratory, and still three (3) microbiology laboratory persons. These persons are needed to be able to carry out the activities for a good laboratory service, and quality which is in accordance with the related applicable standards.

scholarly journals Profile of D-dimer in Uncomplicated Pregnancy

2019 ◽  
pp. 283-287
Author(s):  
Rahajuningsih Dharma ◽  
Mercy T. Panjaitan ◽  
Kanadi Sumapradja ◽  
Rianto Setiabudy
Keyword(s):  
Pregnant Women ◽  
Clinical Pathology ◽  
Cross Sectional Study ◽  
Control Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Uncomplicated Pregnancy ◽  
D Dimer

Abstract Objective: To obtain the profile of D-dimer in uncomplicated pregnancy. Methods: A cross sectional study was done on 90 uncomplicated pregnant women consisted of 30 women in each trimester and 30 healthy, nonpregnant women as control group from July to August 2012. D-dimer level was measured by particle enhanced immunoturbidimetry method using Innovance D-dimer and Sysmex CA 1500 in the Department of Clinical Pathology, Dr. Cipto Mangunkusumo Hospital, Jakarta. Results: All women in the control group showed normal D-dimer level (<0.,5 mg/L FEU). The median and range of D-dimer level in the 1st trimester, 2nd trimester, and 3rd trimester were 0.42 mg/L FEU and 0.1-1.07 mg/L FEU, 0.97 mg/L FEU and  0.6-3.34 mg/L FEU, and 1.56 mg/L FEU and  0.69-3.75 mg/L FEU, respectively.  Increased D-dimer level was found in 27% of pregnant women in 1st trimester, 87% in 2nd trimester, and 100% in 3rd trimester. Conclusion: Increased D-dimer level was found in  27% of pregnant women in 1st trimester, 87% in 2nd trimester, and  100% in 3rd trimester. The range of D-dimer level in the 1st trimester was 0.1-1.07 mg/L FEU, in the 2nd trimester was 0.6-3.34 mg/L FEU, and in the 3rd trimester was 0.69-3.75 mg/L FEU. Keywords: D-dimer, trimester, uncomplicated pregnancy   Abstrak Tujuan : Untuk mendapatkan profil  D-dimer pada kehamilan tanpa komplikasi. Metode : Penelitian potong lintang dilakukan pada 90 perempuan hamil tanpa komplikasi yang terdiri atas 30 perempuan pada tiap trimester dan 30 perempuan sehat yang tidak hamil, sebagai kelompok kontrol dari bulan Juli sampai Agustus 2012. Kadar D-dimer diukur dengan cara particle enhanced immunoturbidimetry  menggunakan reagen InnovanceÒ D-dimer dan koagulometer SysmexÒ CA 1500 di  Deparemen Patologi Klinik, Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo, Jakarta. Hasil: Seluruh perempuan dalam kelompok kontrol mempunyai kadar D-dimer dalam batas normal (<0.,5 mg/L FEU). Median (rentang) kadar D-dimer  pada trimester pertama, kedua, dan ketiga berturut-turut  0.42 mg/L FEU  (0.1-1.07 mg/L FEU), 0.97 mg/L FEU (0.6-3.34 mg/L FEU), dan 1.56 mg/L FEU   (0.69-3.75 mg/L FEU).  Peningkatan kadar D-dimer ditemukan pada 27% perempuan hamil trimester pertama, 87%  trimester kedua, dan pada 100%  trimester ketiga.   Kesimpulan: Peningkatan kadar  D-dimer ditemukan pada  27% perempuan hamil trimester pertama,  87% trimester kedua dan   100% pada trimester ketiga.  Rentang kadar D-dimer level pada trimester pertama adalah 0.1-1.07 mg/L FEU, pada trimester kedua  0.6-3.34 mg/L FEU, dan pada trimester ketiga  0.69-3.75 mg/L FEU. Kata kunci: D-dimer, kehamilan tanpa komplikasi, trimester

Sign in / Sign up

Export Citation Format

Share Document