scholarly journals Evaluation of Ascorbic Acid Adjuvant Therapy and Oxidative Stress Parameters in Burns Patients

2020 ◽  
pp. 296-306
Author(s):  
A. D. G. Nwosu ◽  
E. N. Ossai ◽  
O. Onwuasoigwe ◽  
F. N. Ahaotu ◽  
J. K. Anieze ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Adjuvant Therapy ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Controlled Study ◽  
High Dose ◽  
Intravenous Ascorbic Acid ◽  
Total Antioxidant ◽  
The Impact

Background: Burns trauma is associated with considerable morbidity and mortality. Resuscitation of burns patients with high-dose of Ascorbic acid has shown potential for mitigating the injury, but the optimal dose for this indication is unknown. Aim: This study aimed to evaluate the impact of adjuvant therapy with intravenous Ascorbic acid (6g over 24 hours) on indicators of oxidative stress in patients with major burns. Materials and Methods: We conducted a randomized placebo-controlled study on patients with major burns who presented at the National Orthopaedic Hospital, Enugu, Nigeria between August 2017 and July 2020. Each patient in the treatment group received intravenous Ascorbic acid, 6g over 24 hours, while those in the placebo group received Normal saline in the resuscitation fluid. Oxidative stress evaluation was based on measurement of total antioxidant capacity and malondialdehyde in the participants. The level of statistical significance was determined by a p value of <0.05. Results: The study was conducted on 37 burns patients and 15 healthy subjects. At presentation, the burns patients had significantly lower total antioxidant capacity; P=0.006, and higher serum malondialdehyde; P=0.040, compared to the healthy volunteers. The decrease in serum malondialdehyde in the burns patients treated with high-dose Ascorbic acid; 0.9±0.8 nmol/mL, was greater than that in those treated with placebo; 0.3±1.4 nmol/mL. Similarly the increase in total antioxidant capacity in the burns patients treated with high-dose Ascorbic acid; 151.7±116.5 µmol/L was greater than that in those treated with placebo; 58.4±219.1 µmol/L. However these effects weren’t statistically significant. Conclusion: Intravenous Ascorbic acid at a dose of 6g over 24 hours did not significantly alter the indicators of oxidative stress in the burns patients, under the prevailing conditions of the study.

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

scholarly journals Ascorbic Acid Prevents Vascular Endothelial Dysfunction Induced by Electronic Hookah (Waterpipe) Vaping

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Mary Rezk‐Hanna ◽  
Douglas R. Seals ◽  
Matthew J. Rossman ◽  
Rajat Gupta ◽  
Charlie O. Nettle ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Ascorbic Acid ◽  
Endothelial Function ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Plasma Nicotine ◽  
Before And After ◽  
Total Antioxidant ◽  
Exhaled Carbon Monoxide

Background Electronic hookah (e‐hookah) vaping has increased in popularity among youth, who endorse unsubstantiated claims that flavored aerosol is detoxified as it passes through water. However, e‐hookahs deliver nicotine by creating an aerosol of fine and ultrafine particles and other oxidants that may reduce the bioavailability of nitric oxide and impair endothelial function secondary to formation of oxygen‐derived free radicals. Methods and Results We examined the acute effects of e‐hookah vaping on endothelial function, and the extent to which increased oxidative stress contributes to the vaping‐induced vascular impairment. Twenty‐six healthy young adult habitual hookah smokers were invited to vape a 30‐minute e‐hookah session to evaluate the impact on endothelial function measured by brachial artery flow‐mediated dilation (FMD). To test for oxidative stress mediation, plasma total antioxidant capacity levels were measured and the effect of e‐hookah vaping on FMD was examined before and after intravenous infusion of the antioxidant ascorbic acid (n=11). Plasma nicotine and exhaled carbon monoxide levels were measured before and after the vaping session. Measurements were performed before and after sham‐vaping control experiments (n=10). E‐hookah vaping, which increased plasma nicotine (+4.93±0.92 ng/mL, P <0.001; mean±SE) with no changes in exhaled carbon monoxide (−0.15±0.17 ppm; P =0.479), increased mean arterial pressure (11±1 mm Hg, P <0.001) and acutely decreased FMD from 5.79±0.58% to 4.39±0.46% ( P <0.001). Ascorbic acid infusion, which increased plasma total antioxidant capacity 5‐fold, increased FMD at baseline (5.98±0.66% versus 9.46±0.87%, P <0.001), and prevented the acute FMD impairment by e‐hookah vaping (9.46±0.87% versus 8.74±0.84%, P =0.002). All parameters were unchanged during sham studies. Conclusions E‐hookah vaping has adverse effects on vascular function, likely mediated by oxidative stress, which overtime could accelerate development and progression of cardiovascular disease. Registration URL: https://ClinicalTrials.gov . Unique identifier: NCT03690427.

scholarly journals Influence of Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Oxidative Stress Intensity in Minimally Conscious State Patients

2020 ◽  
Vol 9 (3) ◽  
pp. 683
Author(s):  
Katarzyna Jezierska-Wozniak ◽  
Emilia Sinderewicz ◽  
Wioleta Czelejewska ◽  
Pawel Wojtacha ◽  
Monika Barczewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Minimally Conscious State ◽  
Conscious State ◽  
Total Antioxidant ◽  
Minimally Conscious

Neurological disorders, including minimally conscious state (MCS), may be associated with the presence of high concentrations of reactive oxygen species within the central nervous system. Regarding the documented role of mesenchymal stem cells (MSCs) in oxidative stress neutralization, the aim of this study is to evaluate the effect of bone marrow-derived MSC (BM-MSC) transplantation on selected markers of oxidative stress in MCS patients. Antioxidant capacity was measured in cerebrospinal fluid (CSF) and plasma collected from nine patients aged between 19 and 45 years, remaining in MCS for 3 to 14 months. Total antioxidant capacity, ascorbic acid and ascorbate concentrations, superoxide dismutase, catalase, and peroxidase activity were analyzed and the presence of tested antioxidants in the CSF and plasma was confirmed. Higher ascorbic acid (AA) content and catalase (CAT) activity were noted in CSF relative to plasma, whereas superoxide dismutase (SOD) activity and total antioxidant capacity were higher in plasma relative to CSF. Total antioxidant capacity measured in CSF was greater after BM-MSC transplantations. The content of ascorbates was lower and CAT activity was higher both in CSF and plasma after the administration of BM-MSC. The above results suggest that MSCs modulate oxidative stress intensity in MCS patients, mainly via ascorbates and CAT activity.

scholarly journals Assessment of the Impact of Oxidative Stress on Frozen Seminal Plasma in Fertile and Infertile Men by Examining the Total Antioxidant Capacity

2014 ◽  
Vol 7 (1) ◽  
pp. 25-30
Author(s):  
Violeta S. Rilcheva ◽  
Nina P. Ayvazova ◽  
Danail I. Martinov ◽  
Cvetomir I. Ivanov ◽  
Emiliana I. Konova
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Seminal Plasma ◽  
Total Antioxidant Capacity ◽  
Dna Integrity ◽  
Integrity Test ◽  
Infertile Men ◽  
Total Antioxidant ◽  
The Impact ◽  
Infertile Patients

AbstractThe aim of the study was to explore the impact of oxidative stress on frozen seminal plasma in fertile and infertile men by examining the total antioxidant capacity. Patients: Infertile patients from male infertility clinic with various diagnoses and fertile men. Design: Seminal plasma from proven fertile men [n=50] and infertile patients [n=50] were examined for total antioxidant capacity (TAC) level, semen parameters such as morphology, motility and concentration, and DNA integrity test. Interventions: Seminal plasma TAC measurement by luminometric assay using the TAC assay kit, semen analysis parameters, DNA integrity test. Fertile men showed higher TAC values (median and SD): 1201µM (SD±548), as compared with the infertile patients: 831μM (SD±343). The result from sperm morphology of fertile patients showed a mean percentage of 4.8 % (SD±1.68) whereas the percentage in the infertile group was 2.68% (SD ±1.68). The same group of samples, analyzed for DNA damage showed a mean of DFI 10.38% (SD±5.17%) in fertile men and a mean of DFI 17.22% (SD±7.22%) in infertile men. Total antioxidant capacity of the seminal plasma as measured by the luminоmetric assay is a reliable and simple test for diagnosing and management of male infertility.

scholarly journals The effects of grape seed extract supplementation on exercise-induced oxidative stress in young untrained males

2022 ◽  
Author(s):  
Hamid Reza Zolfi ◽  
Vahid Sari-Sarraf ◽  
Hossein Babaei ◽  
Amirmansour Vatankhah
Keyword(s):  
Oxidative Stress ◽  
Aerobic Exercise ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Controlled Study ◽  
Total Antioxidant ◽  
Exercise Induced

Background and Purpose: The aim of this study was to determine the effect of acute aerobic exercise and 14-days grape seed extract supplementation on total antioxidant capacity, lipid peroxidation and muscle cell damage biomarkers in untrained males Materials and Methods: In a randomized, double-blinded, and placebo-controlled study, twenty-two male students (age 19±1 years, weight 67.44±7 kg, BMI 22±2, V̇ O2max 39±2 ml/kg-1 /min-1 ) were randomly assigned to two groups of grape seed extract and placebo (PLA) (GSE: 200 mg/day for two weeks). After subsequent 14-days of supplementation, subjects did a single session of aerobic exercise (running) on treadmill at 75% VO2max for 30 minutes. Blood samples were taken 3 times: prior to supplementation (baseline), 14-days after supplementation, and immediately after exercise (post-exercise). Total antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), lactate dehydrogenase (LDH) and lactate were measured. TAC was determined by ABTs method. The collected data were then analyzed by running analysis of variance (ANOVA) with repeated measure and Bonferroni post-hoc tests as appropriate using SPSS17 at p<0.05. Results: Malondialdehyde, CK, LDH, Lactate were significantly increased after aerobic exercise (p<0.05). Short-term GSE supplementation significantly prevented MDA and CK cascade after exercise compared to PLA (p<0.05) but, it had no significant effect on basal parameters (p>0.05). The exercise had also no significant effect on total antioxidant capacity in any of the groups (p>0.05). Conclusion: Aerobic exercise could increase blood oxidative stress biomarkers and GSE supplementation, due to influential antioxidant effect; yet, it could attenuate exercise-induced oxidative stress in men

scholarly journals An Impact of Psychological Stress on the Interplay between Salivary Oxidative Stress and the Classic Psychological Stress-Related Parameters

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Nikola M. Stojanović ◽  
Pavle J. Randjelović ◽  
Dragana Pavlović ◽  
Nenad I. Stojiljković ◽  
Ivan Jovanović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Psychological Stress ◽  
Total Antioxidant Capacity ◽  
Mental Stress ◽  
Test Battery ◽  
Total Antioxidant ◽  
Nitrite Nitrate ◽  
The Impact ◽  
Stress Parameters

Both oxidative and psychological (mental) stress are the likely culprits for several acute and chronic health disturbances, and adequate tests mimicking that are needed. Herein, in controlled laboratory surroundings, a PEBL (Psychology Experiment Building Language) test battery was used to evoke stress-related biological responses followed by tracking changes in saliva parameters. The study objectives were to determine the impact of psychological stress on selected salivatory parameters and to assess the correlation between the determined oxidative and stress parameters. The study was conducted on 36 healthy young subjects, mainly females ( n = 24 ). Before and following the completion of a battery of four PEBL tests, subjects’ saliva samples were collected. Stress-evoking changes in total antioxidant capacity and nitrite/nitrate levels, as oxidative stress parameters, and cortisol and immunoglobulin A (IgA), as parameters of psychological stress, were established and mutually correlated by comparing the values of the evaluated parameters pre- and post-PEBL test. The results showed that there is no change in the total salivary antioxidant capacity ( p > 0.05 ); however, there was a significant increase in nitrites/nitrates levels after the PEBL test ( p = 0.007 ). On the other hand, the determined cortisol levels after the test battery were found to be statistically significantly increased ( p = 0.025 ) when compared to the values obtained before the test, while the levels of IgA were found to be statistically significantly decreased ( p < 0.001 ). The only statistically significant correlation between the changes in the studied parameters was found to be the one between cortisol and IgA levels (Spearman’s Rö = -0.4). These results suggest that the short-term stress induced by the PEBL test does evoke changes in the salivary mental stress-related parameters (an increase in cortisol and nitrite/nitrate levels, and a decrease in IgA), but not in the total antioxidant capacity. They also indicate that the constructed PEBL four-test battery might represent an adequate laboratory stress-inducing paradigm.

scholarly journals Antioxidant Potential of Curcumin—A Meta-Analysis of Randomized Clinical Trials

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1092
Author(s):  
Karolina Jakubczyk ◽  
Aleksandra Drużga ◽  
Janda Katarzyna ◽  
Karolina Skonieczna-Żydecka
Keyword(s):  
Oxidative Stress ◽  
Clinical Trials ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Randomized Clinical Trials ◽  
Meta Analysis ◽  
Antioxidant Potential ◽  
Average Dose ◽  
Total Antioxidant ◽  
The Impact

Background: Antioxidant potential is defined as the ability to neutralize oxygen free radicals that are generated in excess due to environmental influences. The body’s defense mechanisms often require support in preventing the effects of oxidative stress. The literature data suggest that curcumin has antioxidant activity that can significantly reduce oxidative stress levels. The aim was to assess the impact of curcumin on oxidative stress markers. Methods: PubMed and Embase were searched from database inception until 27 September 2019 for randomized clinical trials in >20 patients treated with curcumin supplements and randomized to placebo/no intervention/physical activity to verify the antioxidant potential of curcumin. Results: Four studies were included in the meta-analysis, three of which were double-blind and one single-blind. A total of 308 participants took part in the research. A total of 40% of the respondents were men. The average age of participants was 27.60 ± 3.79 years. The average supplementation time was 67 days and the average dose of curcumin administered was 645 mg/24 h. Curcumin significantly increased total antioxidant capacity (TAC) (SMD = 2.696, Z = 2.003, CI = 95%, p = 0.045) and had a tendency to decrease malondialdehyde (MDA) concentration (SMD = −1.579, Z = −1.714, CI = 95%, p = 0.086). Conclusions: Pure curcumin has the potential to reduce MDA concentration and increase total antioxidant capacity.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

scholarly journals Total Antioxidant Capacity in HBV Carriers, a Promising Biomarker for Evaluating Hepatic Fibrosis: A Pilot Study

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Jing-Hua Wang ◽  
Sung-Bae Lee ◽  
Dong-Soo Lee ◽  
Chang-Gue Son
Keyword(s):  
Oxidative Stress ◽  
Chronic Hepatitis ◽  
Liver Fibrosis ◽  
Hepatitis B ◽  
Antioxidant Capacity ◽  
Chronic Hepatitis B ◽  
Hepatic Fibrosis ◽  
Total Antioxidant Capacity ◽  
Hbv Dna ◽  
Total Antioxidant

Oxidative stress plays a pivotal role in the progression of chronic hepatitis B; however, it is unclear whether the status of blood oxidative stress and antioxidant components differs depending on the degree of hepatic fibrosis. To explore the relationship between oxidative stress/antioxidant capacity and the extent of hepatic fibrosis, fifty-four subjects with liver fibrosis (5.5 ≤ liver stiffness measurement (LSM) score ≤ 16.0 kPa) by chronic hepatitis B virus (HBV) were analyzed. From the analysis of eight kinds of serum oxidative stress/antioxidant profiles and liver fibrosis degrees, the level of total antioxidant capacity (TAC) reflected a negative correlation with the severity of hepatic fibrosis (Pearson correlation, r = −0.35, p = 0.01). Moreover, TAC showed higher sensitivity (73.91%) than the aspartate transaminase (AST) to platelet ratio index (APRI, 56.52%) in the receiver operating characteristic (ROC) curves. Interestingly, the TAC level finely reflected the fibrosis degree in inactive carriers (HBV DNA < 2000 IU/mL), while the APRI did in active carriers (HBV DNA > 2000 IU/mL). In conclusion, TAC is a promising biomarker for evaluating the progression of liver fibrosis in patients with HBV, and this finding may indicate the involvement of TAC-composing factors in the pathogenesis of hepatic fibrosis in chronic HBV carriers.

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