scholarly journals Antioxidant and Antibacterial Activity of Anacardium occidentale and Psidium guajava Methanolic Leaf Extracts

2020 ◽  
pp. 29-37
Author(s):  
Mutiat Adetayo Omotayo ◽  
Mary Oluwatoyin Avungbeto ◽  
Oluwole Olusoji Eleyowo
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Psidium Guajava ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Absorbing Materials ◽  
Laboratory Technology ◽  
Β Carotene

Aim: This study evaluates the antioxidant and antibacterial activity of Anacardium occidentale and Psidium guajava methanolic leaf extracts. Place and Duration of Study: The study was carried out in the Biochemistry and Microbiology Laboratory, Department of Science Laboratory Technology, School of Pure and Applied Science, Lagos State Polytechnic, Ikorodu, Lagos- Nigeria for the period of three months between August and October 2015. Methodology: Lycophene and β-carotene was assessed using the method of Nagata and Yamashita while total phenolic and total flavonoid content was assessed by the Folin-Ciocalteau assay and aluminum chloride colorimetric assay respectively. The antioxidant activity was evaluated using the DPPH radical scavenging activity. Antimicrobial activity was assessed by the agar well diffusion technique and mode of action was evaluated by studying the leakage of UV260 and UV280 absorbing materials spectrophotometrically. Results: A. occidentale and P. guajava methanolic leaf extracts evaluated in this study possessed significant amount of antioxidant compounds lycophene, β-carotene, total phenol and flavonoids. The extracts exhibited antioxidant activity by scavenging DPPH radicals in a dose dependent pattern with IC50 of 47.45, 43.49, 41.46 and 27.21 μg/mL for A. occidentale, P. guajava, vitamin C and Gallic acid respectively. Also, the plant extracts exhibited antimicrobial activity against E. coli, S. aureus, P. auraginosa and C. albicans and disrupted microbial membrane evident in the increase in absorbance values of UV260 and UV280 absorbing materials with time. Conclusion: A. occidentale and P. guajava methanolic leaf extracts possess antioxidant and antimicrobial activity and serve as potential source of drugs.

scholarly journals Antioxidant Activity and Antibacterial Effects on Clinical IsolatedStreptococcus suisandStaphylococcus intermediusof Extracts from Several Parts ofCladogynos orientalisand Their Phytochemical Screenings

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pongtip Sithisarn ◽  
Piyanuch Rojsanga ◽  
Patchima Sithisarn ◽  
Sumet Kongkiatpaiboon
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Performance ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Staphylococcus Intermedius

Thein vitroantioxidant and antibacterial assays against clinically isolatedStreptococcus suisandStaphylococcus intermediusof the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalisZipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted. Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC50values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity againstS. intermediuswith the inhibition zones between 7 and 14 mm. The chromatographic data suggested that the leaf extracts ofC. orientaliscontained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity againstStaphylococcus intermedius.

scholarly journals Total Phenolic Content and Antioxidant Activity of Myrtle (Myrtus communis) Extracts

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Mahassine Amensour ◽  
Esther Sendra ◽  
Jamal Abrini ◽  
Samira Bouhdid ◽  
José Angel Pérez-Alvarez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Myrtus Communis ◽  
Leaf Extracts ◽  
Berry Extracts ◽  
Β Carotene ◽  
Linoleic Acid Assay

The total phenolic content and antioxidant activity of methanolic, ethanolic and aqueous extracts of myrtle ( Myrtus communis) leaves and berries were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by the Folin-Ciocalteau assay, while the antioxidant activity was evaluated by three methods: diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, the reducing antioxidant power assay and β-carotene linoleic acid assay. The total phenol content of myrtle extracts ranged between 9.0 and 35.6 mg GAE per g extract. For each solvent, leaf extracts contained significantly higher amount of total phenolic compounds than berry extracts. All of the extracts presented antioxidant capacity assessed by the three methods, but at different levels depending on the concentration, the extraction solvent and the part of the plant used. Generally, leaf extracts showed higher antioxidant activities than berry extracts, while the overall antioxidant strength was in the order methanol > water > ethanol in leaf extracts and methanol > ethanol >water in berry extracts. The phenolic content exhibited a positive correlation with the antioxidant activity: DPPH assay showed the highest correlation (r = 0.949), followed by the reducing power assay (r = 0.914) and the lowest for the β-carotene linoleic acid assay (r = 0.722).

scholarly journals Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6498
Author(s):  
Ahmed S. Abdelbaky ◽  
Abir M. H. A. Mohamed ◽  
Salman S. Alharthi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Rosa Gallica

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of Rosa gallica var. aegyptiaca leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis—and the fungus Candida albicans, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-O-α-d-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of R. gallica var. aegyptiaca leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.

Phytochemical Analysis and Evaluation of Antimicrobial Activity of Peumus boldus, Psidium guajava, Vernonia polysphaera, Persea Americana, Eucalyptus citriodora Leaf Extracts and Jatropha multifida Raw Sap

2019 ◽  
Vol 20 (5) ◽  
pp. 433-444 ◽  
Author(s):  
Jhonatas Emílio Ribeiro da Cruz ◽  
Joyce Ferreira da Costa Guerra ◽  
Marcos de Souza Gomes ◽  
Guilherme Ramos Oliveira e Freitas ◽  
Enyara Rezende Morais
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Medicinal Plants ◽  
Psidium Guajava ◽  
Persea Americana ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Eucalyptus Citriodora ◽  
Leaf Extracts ◽  
Jatropha Multifida

Background: Currently, the treatment of infectious diseases has not always been successful due to the emergence of microbial resistance worldwide. Objective: This study aimed to evaluate the antioxidant activity, content of total phenolic compounds and flavonoids, antifungal potential and antibacterial action of six medicinal plants found in the Cerrado, leaf extracts of Boldo (Peumus boldus), Goiaba (Psidium guajava), Assa-Peixe (Vernonia polysphaera), Abacate (Persea americana), Eucalipto (Eucalyptus citriodora) and raw sap of Bálsamo (Jatropha multifida). Methods: The antioxidant activity was also determined through the DPPH, ABTS and phosphomolybdenum assays. In addition, the total phenolic content and flavonoid dosage were analyzed using the Folin- Ciocalteu method and the aluminum chloride test, respectively. Results: All extracts, except from Assa-Peixe, showed promising values against Staphylococcus aureus, with halos varying from 13-20 mm. Analysis of the minimum inhibitory concentration (MIC) values of the six medicinal plants revealed inhibitory activity of S. aureus, with concentrations varying from 3.12-12.5 mg/mL, which is a significant result considering that S. aureus is one of the main causes of hospital infections. Conclusion: In the analysis of the phytochemical profile, Goiaba contained the best yield of phenolic compounds and total flavonoids, as well as higher antioxidant activity by DPPH and phosphomolybdenum, demonstrating that this species contains antioxidant components that can sequester free radicals under in vitro conditions. Therefore, the crude extracts investigated are promising and their antibacterial and antioxidant actions should be thoroughly studied.

scholarly journals Journal of Food Quality Evaluation of Effect of Extraction Solvent on Selected Properties of Olive Leaf Extract

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Won-Young Cho ◽  
Da-Hee Kim ◽  
Ha-Jung Lee ◽  
Su-Jung Yeon ◽  
Chi-Ho Lee
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Distilled Water ◽  
Olive Leaf ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Olive Leaf Extract

The quest for natural preservatives and functional foods with health benefits has seen an increasing demand for natural products having therapeutic value. Herein, we investigated the influence of ethanol, methanol, acetone (50%, 70%, and 90% v/v), and distilled water on selected properties of olive leaf extract and determined the yield, total phenolic content (TPC), antioxidant activity, and antimicrobial activity. Extracts were analyzed for their oleuropein, hydroxytyrosol, and tyrosol contents by high-performance liquid chromatography (HPLC). The highest extraction yield of 20.41% was obtained when using 90 vol% methanol, while the highest total polyphenol contents of 232 and 231 mggallic-acid-equivalent/100 g were obtained for 90 vol% methanol and 90 vol% ethanol, respectively. Antioxidant activity was determined using the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging assay, by determining the ferric reducing antioxidant power (FRAP), and using the Fe2+-chelating activity assay, which provided the highest values when 90 vol% methanol was used (33.84%, 0.75, and 12.91%, respectively). HPLC analysis showed that the highest oleuropein contents corresponded to the extracts obtained using 90 and 70 vol% methanol (26.10 ± 0.20 and 24.92 ± 1.22 g/L, respectively), and the highest antimicrobial activity was observed for 90 vol% methanol and distilled water. Olive leaf extracts using 90 vol% methanol had high levels of polyphenols and were highly antioxidant and antimicrobial. The results of this study facilitate the commercial applications of natural extracts with antioxidant and antibacterial activities and are expected to establish a foundation for further optimization studies.

scholarly journals Chemical Composition and In Vitro Antioxidant and Antimicrobial Activities of Marrubium vulgare L.

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ibrahim Mssillou ◽  
Abdelkrim Agour ◽  
Noureddine Hamamouch ◽  
Badiaa Lyoussi ◽  
Elhoussine Derwich
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Total Antioxidant ◽  
Marrubium Vulgare ◽  
Antioxidant And Antimicrobial Activities

In this study, the polyphenol content and the antioxidant and antimicrobial activities of hydroethanolic (MVE) and hydroacetonic (MVA) leaf extracts of Marrubium vulgare L. were examined. The results indicated that the total phenolic content was higher in MVA (112.09 ± 4.77 mg GAE/DW) compared to MVE extract (98.77 ± 1.68 mg GAE/DW). The total flavonoid content was also higher in MVA extract (21.08 ± 0.38 mg QE/g DW) compared to MVE (17.65 ± 0.73 mg QE/g DW). Analysis of the chemical composition revealed the presence of 13 compounds with a total of 96.14%, with the major compound being malic acid (22.57%). Both extracts possess a good total antioxidant activity. DPPH and FRAP assays indicated that the MVE extract possesses a better antioxidant activity, with IC50 = 52.04 µg/mL ± 0.2 and EC50 of 4.51 ± 0.5 mg/mL, compared to MVA extract (IC50 = 60.57 ± 0.6 µg/mL and EC50 of 6.43 ± 0.0411 mg/mL). Moreover, both extracts exhibited strong antimicrobial activity against certain nosocomial strains as indicted by the MIC values, which ranged between 0.93 mg/mL and 10 mg/mL. Taken together, these results reveal the importance of M. vulgare as a natural antioxidant with important antimicrobial activity.

scholarly journals Bioactive properties of Persea lingue Ness (Lauraceae) fruit and leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. Lillo ◽  
F. Carvajal-Caiconte ◽  
W. Vital ◽  
P. I. Silva Junior ◽  
E. J. Paredes-Gamero ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Leaf Extract ◽  
Leukemia Cell ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Fruit Extract ◽  
South Central

Abstract Persea lingue Ness is a tree species that lives mainly in temperate forests of south-central Chile. Its leaves are used in ethnomedicine, the fruit is a drupe similar to that of the avocado and has not been studied. The aim of this study was to determine the cytotoxicity in leukemia cell and antibacterial activity, along with some chemical content characteristics of P. lingue fruit and leaf extracts. The antibacterial activity was determined by the inhibition of bacterial growth in liquid medium assay against Gram-positive and Gram-negative bacteria. The leukemia cell lines Kasumi-1 and Jurkat were used to evaluate the cytotoxic activity by using propidium iodide and AlamarBlue assays. Total phenolic, flavonoid, condensed tannin, alkaloid and lipid contents were evaluated in the fruit and in the leaf extracts. The antioxidant activity of both extracts were also elavaluated. Leaf extract presented the highest content of total phenols, condensed tannins and flavonoids, and also the highest antioxidant activity. While the fruit extract has a higher amount of lipids and alkaloids and the high antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus megaterium and Micrococcus luteus. The leaf extract only showed activity against M. luteus. Concerning the cytotoxic activity, only the fruit extract showed cytotoxicity against the cell lines Jurkat and Kasumi-1. P. lingue fruit extract is a potential source of biologically active molecules for the development of new drugs to be used in some types of leukemia, as well as antibacterial agent.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF ANACARDIUM OCCIDENTALE

2018 ◽  
Vol 11 (12) ◽  
pp. 474
Author(s):  
Shobha Kl ◽  
Amita Shobha Rao ◽  
Pai Ksr ◽  
Sujatha Bhat
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Bacterial Infections ◽  
Ethanolic Extract ◽  
Culture Collection ◽  
Anacardium Occidentale ◽  
Diffusion Method ◽  
Leaf Extracts

Objective: The objective of this study was to evaluate the antimicrobial activity of leaves of Anacardium occidentale (A. occidentale) against microorganisms including multidrug-resistant (MDR) bacteria. Methods: Agar well diffusion method was employed to demonstrate the antimicrobial activity of leaves A. occidentale. Ethanol and aqueous extracts of the leaves were used against microorganisms, which included American type culture collection strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa and the clinical isolates of Streptococcus pneumoniae, Candida albicans, MDR Escherichia coli, and MDR Klebsiella pneumoniae. Results: The ethanolic extract of leaves of A. occidentale showed significant antimicrobial activity. Aqueous extract had mild antifungal activity. Conclusion: Ethanolic extract of leaves of A. occidentale could be a good source for the antibacterials to combat MDR bacterial infections. Further studies are necessary for these potent plant extracts to evaluate the in vivo efficacy and toxicity.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

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