scholarly journals Antioxidant Potentials of Pentaclethra macrophylla Seed (Ugba) on Mercury Toxicity Induced Hepatic, Renal and Testicular Oxidative Stress in Male Albino Rats

2021 ◽  
pp. 157-167
Author(s):  
J. D. Nwahiri ◽  
D. G. Tamuno-Emine ◽  
E. O. Nwachuku ◽  
E. S. Bartimaeus
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Standard Dose ◽  
Albino Rats ◽  
Mercury Chloride ◽  
Chloride Salt ◽  
Plant Seed ◽  
Port Harcourt ◽  
Animal House ◽  
Rivers State

Aim: This study aimed to evaluate the antioxidant potentials of Pentaclethra macrophylla seed (Ugba) on mercury-induced hepatic, renal and testicular oxidative stress in male Albino Rats. Study Design: This study is an experimental study. Place and Duration of Study: Department of Animal and Environmental Biology Animal House, Rivers State University, Port Harcourt, Nigeria, between January 2019 and January 2021. Methodology: Thirty-six (36) adult male albino rats weighing approximately 135±1.5g were purchased from the University of Port Harcourt, Rivers State, Nigeria. They were housed in plastic suspended cages, placed in well-ventilated conditions and provided with rat diet and water, and acclimatized for two weeks. Fresh matured seeds of Pentaclethra macrophylla (African oil bean seed) were sourced locally from markets in Imo state, Nigeria.  The maceration technique was used to carry out an ethanolic extract of the plant seed. Mercury chloride salt was purchased from Port Harcourt. A standard dose of 3.0mg/kg body weight of mercury chloride obtained from acute toxicity study dose determination was administered to the rats for 30 days after they were divided into six groups of six rats per group. After 30 days, all the animals were weighed, anesthetized using chloroform. Tissue samples of liver, kidney, and testis were collected and homogenized in phosphate-buffered saline and stored at 40C for determination of oxidative stress assessment. Malondialdehyde, Reduced Glutathione (GSH), Catalase Activity, superoxide dismutase (SOD) activity, Glutathione Peroxidase Activity were analyzed using the homogenized sample. Data were expressed as mean ±SD, and the statistical analysis was performed with the SPSS statistics 23.0, and p values less than .05 were considered statistically significant. Results: The results showed that there were significant increases (P<.05) in the liver, kidney, and testicular MDA and significant decreases (p<.05) in SOD, CAT, GSH, and GPx activities in group 2 rats versus control. However, after treatment with different concentrations of Pentaclethra macrophylla, MDA activity significantly decreased (P<.05), while SOD, CAT, GPx, and GSH significantly increased (P<.05). Conclusion: The results obtained from the study showed that mercury chloride has the potential to cause liver, kidney, and testicular toxicity to adult male albino rats, and this toxicity was exerted majorly through oxidative stress and lipid peroxidation. However, treatment with Pentaclethra macrophylla seed extract ameliorated oxidative stress.

6-gingerol ameliorates gentamicin induced renal cortex oxidative stress and apoptosis in adult male albino rats

2016 ◽  
Vol 48 (3) ◽  
pp. 208-216 ◽  
Author(s):  
Ahmed M.S. Hegazy ◽  
Mohammed M. Mosaed ◽  
Saad H. Elshafey ◽  
Naglaa A. Bayomy
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Renal Cortex ◽  
Albino Rats

scholarly journals Evaluation of the Effect of Some Marketed Herbal Cosmetics in Port Harcourt on Renal Parameters of Rabbits

2021 ◽  
pp. 70-77
Author(s):  
I. N. Thompson ◽  
E. S. Bartimaeus ◽  
E. O. Nwachuku ◽  
H. Brown ◽  
E. S. Agoro
Keyword(s):  
Heavy Metals ◽  
The Body ◽  
Early Signs ◽  
Port Harcourt ◽  
Group A ◽  
Continued Use ◽  
Herbal Cosmetics ◽  
Group B ◽  
Animal House ◽  
Rivers State

Aim: The aim of this study was to evaluate the effects of three commonly marketed herbal cosmetics in Port Harcourt on the renal parameters in rabbits. Study Design:  This study is an experimental study. Place and Duration of Study: This study was carried out at Animal House, Applied and Environmental Biology Department, Rivers State University, Port Harcourt, Rivers State, Nigeria, between April 2020 and November 2020. Methodology: A total of 48 rabbits were used for the study. They were divided into four groups with twelve rabbits in each group. Four rabbits from each group were treated for thirty, sixty and ninety days respectively. All the rabbits were given feed and tap water ad-libitum. Using the Organisation for Economic Co-operation and Development (OECD) guideline for volume selection, 0.5ml/kg of Samples A, B, C and D were each applied to 5cm by 5cm scrapped dermal Forsa of the rabbits in each group every morning for the respective treatment periods stated above. At days thirty, sixty and ninety, respectively, four rabbits from each group were sacrificed under chloroform anaesthesia. Blood samples were collected from the rabbits at intervals, 30days, 60days and 90days. The kidneys were harvested at 90 days from the rabbits. The blood was collected to test sodium, potassium, chloride, calcium, urea, creatinine, KIM-1 and kidney for histological analysis. GraphPad Prism v.7.0 was used for statistical analysis and p values less than 0.05 were considered statistically significant. Results: The results showed that Potassium level was significantly higher (p<.05) from day 30 for group A compared to control, group B and group C. This is attributed to group A having a higher level of cadmium and arsenic compared to the other groups.  The urea and creatinine result for group A was significantly higher by day 30 (p<.05) compared to control with group B and group C. Whereas calcium became significantly lower at day 60. With chloride significantly higher (p<.05) at day 60. Early signs of toxicity to the Kidney were identified from the significant effect on the urea, creatinine, KIM-1 and histology results. The continued use of these products contaminated by these heavy metals will release them slowly into the body of recipients and which will invariably damage the kidney. Conclusion: The continued use of these products contaminated by these heavy metals will release them slowly into the body of recipients and which will invariably damage the kidney. Early signs of toxicity to the Kidney were identified from the significant effect on the urea, creatinine, KIM-1 and histology results.

scholarly journals Effect of aqueous solution of caffeine on serum level of superoxide dismutase in male Wister rats

2022 ◽  
Vol 18 (1) ◽  
pp. 046-050
Author(s):  
Chima Daniel ◽  
Onwugbuta Godpower Chukwuemeka ◽  
John Godson Nnamdi
Keyword(s):  
Superoxide Dismutase ◽  
Animal Feed ◽  
The Body ◽  
Albino Rats ◽  
Antioxidant Compounds ◽  
Feed Water ◽  
Port Harcourt ◽  
Dietary Antioxidant ◽  
Animal House

Caffeine is an active ingredient of coffee (Nescafe) as one of the major sources of dietary antioxidant compounds which counteract the action of reactive oxygen species (ROS) which are the main contributors to oxidative stress. This research is carried out to investigate the role of caffeine in modulating superoxide dismutase and caffeine’s contribution to aging. Forty five sexually matured albino rats with weights ranging from 120-200g were purchased from the animal house of the Department of Anatomy, University of Port Harcourt. These animals were divided into five groups according to their weights and were housed in fifteen well ventilated cages containing sawdust which serves as beddings for the comfort of the animals and for easy removal of their feaces. They were fed daily with animal feed, water ad libitum and allowed to acclimatize for a period of one week, after which their weights were taken and caffeine administration commenced.The results obtained showed a significant (p<0.05) increase in the body weight of the animals on days 14 and 21 following the administration of 20mg/kg of the extract when compared with day 0 and a significant (p<0.05) increase in serum superoxide dismutase activity for 100mg/kg on day 14 when compared to the control. The findings of this study suggests that caffeine (NESCAFE) may have little or no significant effect on the activity of superoxide dismutase.

scholarly journals Ameliorating Iron Overload in Intestinal Tissue of Adult Male Rats: Quercetin vs Deferoxamine

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Arwa A. El-Sheikh ◽  
Shimaa Hamed Ameen ◽  
Samaa Salah AbdEl-Fatah
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Adult Male ◽  
Caspase 3 ◽  
Iron Chelation ◽  
Male Rats ◽  
Albino Rats ◽  
Tissue Iron ◽  
Small Intestinal

Objective. The aim of our study is to compare the role of the new natural alternative (Quercetin) with the current iron-chelation therapy (Deferoxamine (DFO)) in the effect of iron overload on small intestinal tissues and to investigate the possible underlying molecular mechanisms of such toxicity. Methods. Forty-two adult male albino rats were divided into six groups: control groups, DFO, Quercetin, iron overload, iron overload+DFO, and iron overload+Quercetin groups. Animals received daily intraperitoneal injection of Deferoxamine (125 mg /kg), Quercetin (10 mg/kg), and ferric dextran (200 mg/kg) for 2 weeks. Results. Iron overloaded group showed significant increase in serum iron, total iron binding capacity (TIBC), transferrin saturation percentage (TS %) hepcidin (HEPC), serum ferritin, nontransferrin bound iron (NTBI), and small intestinal tissues iron levels. Iron overload significantly increased the serum oxidative stress indicator (MDA) and reduced serum total antioxidant capacity (TAC). On the other hand, iron overload increased IL6 and reduced IL10 in small intestinal tissues reflecting inflammatory condition and increased caspase 3 reactivity indicating apoptosis and increased iNOs expressing cell indicting oxidative stress especially in ileum. In addition, it induced small intestinal tissues pathological alterations. The treatment with Quercetin showed nonsignificant differences as compared to treatment with DFO that chelated the serum and tissue iron and improved the oxidative stress and reduced tissue IL6 and increased IL10 and decreased caspase 3 and iNOs expressing cells in small intestinal tissues. Moreover, it ameliorated the iron overload induced pathological alterations. Conclusion. Our study showed the potential role of Quercetin as iron chelator like DFO in case of iron overload induced small intestinal toxicity in adult rats because of its serum and tissue iron chelation, improvement of serum, and small intestinal oxidative stress, ameliorating iron induced intestinal inflammation, apoptosis, and histopathological alterations.

Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Pharmacology ◽  
2019 ◽  
Vol 103 (3-4) ◽  
pp. 202-211 ◽  
Author(s):  
Marwan Abdel-Latif Ibrahim ◽  
Alaa-Eldin Salah-Eldin
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Adult Male ◽  
Rna Extraction ◽  
B Cell Lymphoma ◽  
Experimental Period ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Withdrawal Effect

Aim: The present study aimed to elucidate the effects of tramadol on the testicular functions of adult male rats due to the chronic usage of tramadol and the effect of its withdrawal. Method: Adult male albino rats were classified into the following 3 groups: (I) a control administered with normal saline and (II) tramadol-treated rats (40 mg/kg b.w. orally) for 21 successive days; and (III) like the rats in the second group but kept for 4 weeks after the last tramadol dose to study the effect of tramadol withdrawal. At the end of the experimental period, blood was collected and specimens from testis were taken for histopathological, biochemical, and molecular studies. A reverse transcription-polymerized chain reaction after RNA extraction from specimens was detected for the anti-apoptotic and pro-apoptotic genes in testicular tissues. Also, malondialdehyde (MDA) was measured in tissues homogenate and antioxidant enzymes activities were evaluated. Results: The results of this study demonstrated histological changes in testicular tissues in groups II and III compared to the control group, accompanied with increased apoptotic index and proved by increased B-cell lymphoma-2 (Bcl-2) associated-X-protein and caspase-3 expression, whereas anti-apoptotic Bcl-2 markedly decreased. Moreover, in tramadol-abused and -withdrawal groups, the MDA level increased, while the antioxidant enzymes activity decreased and revealed oxidative stress, indicating that tramadol is harmful at the cellular level and can induce apoptotic changes in testicular tissues. The withdrawal effect showed signs of improvement, but it did not return to normal levels. Conclusions: It could be concluded that the administration of tramadol causes abnormalities on testicular tissues associated with oxidative stress, which confirmed the risk of increased oxidative stress on testicular tissues due to tramadol abuse.

Quinalphos induced oxidative stress and histoarcheitectural alterations in adult male albino rats

2012 ◽  
Vol 34 (3) ◽  
pp. 673-678 ◽  
Author(s):  
Mythily Subramaneyaan ◽  
Smita Jain ◽  
Chandrashekhar Yadav ◽  
Vinod K. Arora ◽  
Basu D. Banerjee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Male ◽  
Albino Rats

Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats

2017 ◽  
Vol 42 (4) ◽  
Author(s):  
Mahgoub Mohamed Ahmed
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Rat Liver ◽  
Adult Male ◽  
Total Protein ◽  
Albino Rats ◽  
Serum Total Protein

AbstractObjective:The objective of the current study is to investigate the protective effect ofMethods:Forty adult male albino rats were divided into four groups as control,Results:The results showed that, administration of EtOH caused a significant decrease (p<0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.Conclusion:had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver.

scholarly journals Possible protective effect of olive leaves extract on paracetamol induced hepatotoxicity in male albino rats

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Mervat EL-Sayed Taha ◽  
Amaal Mohamed Kamal ◽  
Dalia Ramzy Ibrahim
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Glutathione Reductase ◽  
Adult Male ◽  
Total Bilirubin ◽  
Treated Group ◽  
Alpha Fetoprotein ◽  
Male Rats ◽  
Albino Rats ◽  
Olive Leaves

Paracetamol (PCM) overdose can cause hepatotoxicity with oxidative stress; the present study was carried out to establish the possible protective effect of olive leaves extract (OLE) on toxicity induced by paracetamol in adult male rats. Twenty four adult male rats were divided into four equal groups; control, olive leaves extract group, paracetamol group and olive leaves extract plus paracetamol group. Some biochemical parameters and liver histopathology were evaluated. PCM treatment significantly increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), urea, creatinine and alpha-fetoprotein. Paracetamol was found to significantly increase malonaldehyde (MDA) and decrease glutathione reductase (GR) activity in tissue and significantly decrease total antioxidant capacity (TAC) and superoxide dismutase (SOD) in serum. Administration of OLE caused a significant decrease serum AST, ALT enzyme, total bilirubin, GGT, LDH, creatinine, urea, alpha-fetoprotein. Also, amelioration of oxidant – antioxidant status with olive leaves extract was observed in addition to a significant decrease in MDA and a significant increase in TAC in liver tissue with a significant increase in glutathione reductase (GR) and SOD in serum compared to paracetamol treated group The chemical pathological changes were in step with histopathological observation suggesting marked hepatoprotective result of olive leaves extract. It could be concluded that olive leaves extract (OLE) treatment may be effective in decreasing hepatic injury and oxidative stress induced by paracetamol overdose in male albino rats.

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