scholarly journals Effect of Methanolic Extract of Securigera securidaca as Antioxidant and Antibacterial Activities

2020 ◽  
pp. 10-17
Author(s):  
Ghassab M. Al- Mazaideh ◽  
Saleh A. Al- Quran
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Vitro Assay ◽  
Securigera Securidaca

In the present work, the phytochemical screening, polyphenolic content, antibacterial activity and antioxidant activity of Securigera securidaca seeds in methanol were carried out. Phytochemical analysis of seeds showed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids and glycosides. Total phenolic content was estimated by Folin Ciocalteau method and the result showed the highest phenolic content of 62.28 mg/g. Methanolic extract was screened for antibacterial activity by disc diffusion method and it found to be potent. The MIC of methanol extract identified by broth dilution method showed a MIC value of 0.25 mg/ml for both E. coli and Kl. Oxytoca, and also 0.5 mg/ml for both S. aureus and S. epidermis. The antioxidant effect of the seeds was tested by DPPH scavenging activity as in vitro assay. The extract had potent inhibitory activity (IC50) value of 0.057 mg/ml. The finding experimental results showed that methanolic extract of Securigera securidaca is important as a source of antibacterial activity and polyphenolic antioxidants.

Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

2021 ◽  
Vol 2 (1) ◽  
pp. 23-33
Author(s):  
Abdul Aziz ◽  
Veggy Nadya Yuliawan ◽  
Paula Mariana Kustiawan
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Polar Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Content ◽  
Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2  mm ± 1.5) at  200µg/mL concentration.

scholarly journals Antibacterial Potential of some Plant Extracts against Escherichia coli, along with its Phytochemical Analysis, Total Phenolic Content, and Antioxidant Properties

2019 ◽  
Vol 5 (02) ◽  
pp. 96-102
Author(s):  
Pooja Singh ◽  
Upma Srivastava
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis

In vitro antibacterial activity of 21 plant methanolic extracts were investigated by disc diffusion method against gram-negative bacteria Escherichia coli. Amongst the extracts tested, the Datura stramonium L. and Ocimum basilicum L. showed significant antibacterial activity against the bacterial pathogen. D. stramonium showed the highest antibacterial activity followed by O. basilicum extract. Phytochemical analysis of extracts showed the presence of alkaloids, tannins, saponins, flavonoids, and phenols. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ABTS analysis of potent extract D. stramonium showed appreciable antioxidant activity. For DPPH and ABTS assay the highest antioxidant activity was observed at 60 mg ml-1 concentration with a percent inhibition of 71.66 and 77.19 with IC50 value 9.71 and 22.16 μg ml-1 respectively. Moreover, the radical scavenging activity of the extract was lower than that observed for the synthetic antioxidants butylated hydroxyanisole (BHA )and butylated hydroxytoluene (BHT). The total phenolic content of D. stramonium extract was found to be 0.592 mg GAE/gm which was assayed using Folin ciocalteau reagent. The results provide evidence that the extract of D. stramonium and O. basilicum can be further recommended in the treatment of the infections caused by E. coli; further D. stramonium is also a potential source of natural antioxidants.

scholarly journals In vitro antibacterial activity of traditionally used medicinal plants against Xanthomonas campestris pv. musacearum in Ethiopia

2019 ◽  
Vol 20 (2) ◽  
pp. 555-561
Author(s):  
GETAHUN YEMATA ◽  
BRUKTAWIT DESTA ◽  
MASRESHA FETENE
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Xanthomonas Campestris ◽  
Phenolic Content ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
In Vitro Antibacterial Activity

Abstract. Yemata G, Desta B, Fetene M. 2019. In vitro antibacterial activity of traditionally used medicinal plants against Xanthomonas campestris pv. musacearum in Ethiopia. Biodiversitas 20: 555-561. In Ethiopia, traditional medicinal plants have long been used to treat human and livestock ailments. Nevertheless, studies about the use of these plant extracts to control crop diseases are scarce. Therefore, the aim of the present study was to evaluate the antibacterial activity of traditionally used medicinal plants against Xanthomonas campestris pv. musacearum (Xcm). The bioactive chemicals from leaf samples were drawn using methanol by maceration method. Total phenolic content of the extracts was determined by Folin Ciocalteau reagent. The antibacterial activity of leaf extracts was evaluated by disc diffusion method. The phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, terpenoids, tannins and saponins. Extracts with higher total phenolic content had greater antibacterial activity. The extract of each species showed antibacterial activity against Xcm on a dose dependent manner. Significant differences were recorded between species and test concentrations. The extract of Bersama abyssinica exhibited the strongest antibacterial activity at 200 mg/mL followed by Ricinus communis, Eucalyptus citriodora and Acokanthera schimperi. At lower test concentrations, extracts of E. citriodora and R. communis revealed higher antibacterial activity. Moreover, these species had lower bacteriostatic and bactericidal concentrations. The results showed the potential potency of E. citriodora and R. communis leaf extracts in controlling enset bacterial wilt. However, further studies on the identification of chemical compounds and in vivo evaluation of the extracts are recommended.

scholarly journals Giloy (Tinospora Cordifolia): In Vitro Evaluation of Antibacterial Properties

2021 ◽  
Vol 07 (06) ◽  
Author(s):  
Tulsa Devi ◽  
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Hot Water ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Hot Water Extract ◽  
Agar Well Diffusion Method

Antibiotic resistance has become a global concern and hence, the search for other source of antimicrobials initiated to find a way to control infections in future. The main objective of this paper is to screen Giloy (Tinospora cordifolia) for its antibacterial activity. The stem of Tinospora cordifolia is used to prepare extract for determining it’s in vitro antibacterial activity as per the agar well diffusion method. In the agar well diffusion method 100μl of 24 hr broth culture of bacteria was aseptically and evenly swabbed on Mueller Hinton agar plates. Wells of about 8 mm diameter were aseptically cut using sterile cork-borer. 100 μl of plant extracts of different concentrations were then placed into the separate wells. The plates were incubated at 37 oC for 24hr. Antimicrobial activity of the giloy was determined by measuring the diameter of zone of inhibition. The methanolic extract of Tinospora cordifolia showed 13, 11, 9 and 5 mm zone of inhibition in S. aureus cultures by using 100, 75, 50 and 25 mg/ml concentration, respectively while hot water extract of Tinospora cordifolia showed 14, 12, 10 and 8 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25 mg/ml concentration, respectively and the cold extract of Tinospora cordifolia showed 10, 8, 5 and 0 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25mg/ml concentration, respectively. The methanolic extract of Tinospora cordifolia indicated 12, 10, 6 and 4 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively and the hot water extract of Tinospora cordifolia showed 16, 14, 12 and 10 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25mg/ml concentration, respectively. The cold water extract of Tinospora cordifolia showed 13, 10, 8, and 5 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively. It has been observed that Tinospora cordifolia showed very promising results as indicated by the zone of inhibition of bacterial culture through agar well diffusion method that varies from few mm to few cm. This study indicates the in-vitro antibacterial effect of Giloy which needs further validation through in-vivo studies.

ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nur Azfa Shuib ◽  
Anwar Iqbal ◽  
Fatimatul Akmal Sulaiman ◽  
Izzatie Razak ◽  
Deny Susanti
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disc Diffusion ◽  
Flavonoid Content ◽  
Disc Diffusion Method ◽  
Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

scholarly journals Vachellia farnesiana (L.) Wight & Arn. Growing in Bangladesh Exerts In-vitro Antioxidant and In-vivo Analgesic and Anti-diarrheal Activities

2020 ◽  
Vol 23 (2) ◽  
pp. 181-186
Author(s):  
Md Sadman Hasib ◽  
Md Sazzadul Bari ◽  
Akhteruzzaman Chowdhury ◽  
Md Aslam Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Body Weight ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Vitro Assay ◽  
Ic50 Value ◽  
Analgesic Activities

The present study was conducted to evaluate the antioxidant, antidiarrheal and analgesic activities of Vachellia farnesiana (L.) Wight & Arn. The methanol extract of V. farnesiana and its different fractionates were subjected to in-vitro assay for the determination of total phenolic content and antioxidative potential. The ethyl acetate soluble fraction (EASF) exhibited the highest free radical scavenging capacity (IC50 value of 21.49 ± 1.04 μg/ml) as compared to that exhibited by the standard butylated hydroxyl toluene (BHT) (IC50 value of 20.41 ± 0.05 μg/ml). Such prominent antioxidative potential was further reinforced by a phenolic content of 39.26 ± 0.85 mg of gallic acid equivalent per gram of extract. The plant extract, at the dose of 400 mg/kg body weight, reduced castor oil-induced diarrhea in mice model by a statistically significant (p < 0.05) margin of 47.62%, while the standard loperamide produced 66.67% reduction of diarrheal feces. The central and peripheral analgesic activities of the crude methanol extract of V. farnesiana (MEVF) was determined by tail flick- and acetic acidinduced writhing methods, respectively, in Swiss albino mice. In the tail flick method, oral administration of MEVF at doses of 200 and 400 mg/kg body weight exhibited 221.09 and 237.09% elongation of pain response time, respectively, after 90 minutes of administration whereas the standard morphine effectuated 518.34% elongation within the same time. Furthermore, the same doses of the extract illustrated 63.27 and 69.39% reductions, respectively, in the acetic acid-induced abdominal constrictions in mice. Compared to the standard acetylsalicylic acid with 75.51% inhibition, statistically significant (p < 0.05) peripheral analgesic activity was established. The results of the present investigations suggest that methanol extract of V. farnesiana possesses antioxidant, antidiarrheal and analgesic activities which eventually indicates the presence of biologically important phytoconstituents within the plant that needs further exploration. Bangladesh Pharmaceutical Journal 23(2): 181-186, 2020

scholarly journals Determination of Biological Activities of the Root Bark of Senna singueana

2020 ◽  
pp. 25-34
Author(s):  
Kidanemariam Teklay Hilawea ◽  
Zelalem Yibralign Desta
Keyword(s):  
Antibacterial Activity ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Root Bark ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aims: Senna singueana is a medicinal plant which is used for the treatment of different kinds of diseases and the plant was selected for the study because of its numerous uses. The main objective of this study was to determine the antioxidant and antibacterial activity of the extracts of the root barks of S. singueana. Methodology: The antibacterial activities of the extracts (determination of Minimal Inhibitory “MIC” and Minimal Bactericidal Concentration “MBC”) were determined by using agar well diffusion method. In addition to this the total flavonoid and total phenolic contents were determined by using aluminum chloride colorimetric complex assay and Folin-Ciocalteu method respectively. Results: Our results revealed that the total flavonoid content of the extracts is ranged from 30.39 mgQE/100 g to 240.83 mgQE/100 g. The extracts also showed good antioxidant activity and total phenolic content as well as weak to moderate antibacterial activity against some bacteria. Conclusions: The extracts of the root bark of Senna sinueana showed good total flavonoid content, DPPH radical scavenging activity and antibacterial activity. In addition to this, the extracts also showed the presence of some important compounds by phytochemical analysis.

scholarly journals Phenolic Compounds from Psidium guajava (Linn.) Leaves: Effect of the Extraction-Assisted Method Upon Total Phenolics Content and Antioxidant Activity

2020 ◽  
Vol 11 (2) ◽  
pp. 9346-9357
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Crosslinking Agent ◽  
Psidium Guajava ◽  
Total Phenolic ◽  
Vitro Assay ◽  
Ultrasound Assisted ◽  
Guava Leaves

The aim of this study was to investigate the influence of the extraction method on the dry extract yield of guava leaves, correlating the total phenolic content (TPC) with the antioxidant activity. The dry extracts were obtained from hydroethanolic (50 and 70%) extract using the ultrasound-assisted method. Folin-Ciocalteau reagent was used to determine the content of TPC. DPPH (2,2-diphenyl-1-picrylhydrazyl) in vitro assay was used to determine the ability to scavenge free radicals. The results analyses demonstrated that the ultrasound-assisted method produced a higher yield in both dry extracts (11%), in contrast to the conventional method. The 50% hydroethanolic solvent was more efficient in the extraction of bioactive compounds. Both extracts showed a positive correlation of phenolic content with antioxidant activity. The FTIR spectrograms showed changes in the chemical groups, as well as determining the aromaticity index of the extracts, indicating a higher aromatic prevalence to the solvent 50%, although it presented simpler phenolic structures. In conclusion, the results provide an important basis for the use of phenolic compounds extracted from guava leaves, not only due to the antioxidant activity exerted, however, for potential use as a crosslinking agent of sulfated and non-sulfated glycosaminoglycan (GAG).

scholarly journals Pharmacognostical Investigation of Andrographis paniculata (Green Chiretta) and Crystallization of the Bioactive component Andrographolide

2020 ◽  
Vol 13 (2) ◽  
pp. 40-50
Author(s):  
Myrene R. Dsouza ◽  
Sapam Athoibi ◽  
Shashi Prabha
Keyword(s):  
Protein Denaturation ◽  
Linear Regression Analysis ◽  
Andrographis Paniculata ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Rbc Membrane ◽  
Bioactive Component ◽  
Ic50 Values

Andrographis paniculata (Family: Acanthaceae) is one the most commonly used ethno-medicinal plants in certain parts of Asia and European countries. The phytochemical analysis of the leaves of A. paniculata in aqueous, methanolic, ethanolic, hydromethanolic (1:1) and hydroethanolic (1:1) extracts revealed the presence of carbohydrates, amino acid, alkaloids, saponins, tannins, flavonoids, terpenoids, glycosides, xanthoproteins and phenols. The total phenolic, flavonoid contents and FRAP values were found to be highest in the hydromethanolic extract i.e., 0.23 ± 0.008 mg GAE/g of FWt, 0.031± 0.00 mg QE/g FWt and1.261 ± 0.03 mM FeSO4 respectively. Invitro antioxidant capacity by linear regression analysis was measured by assaying DPPH radical and H2O2 scavenging capacities. The respective IC50 values of the hydromethanolic extract of the plant were found to be 86.51 μg/ml and 298.27 μg/ml. The IC50 values for in vitro anti-inflammatory activities were evaluated by heat induced protein denaturation (IC50 diclofenac = 574.06 μg/ml, IC50 APE = 179.7 μg/ml) and RBC membrane stabilization assay (IC50 diclofenac = 337.64 μg/ml, IC50 APE = 143.07 μg/ml). The IC50 values for in vitro anti-diabetic activities were evaluated by α-amylase inhibition (IC50 acarbose = 379.71 μg/ml, IC50 APE = 328.54 μg/ml). In addition, glucose diffusion was also monitored. Antimicrobial activity of the extracts was studied against common pathogens using well diffusion method. The purification of Andrographolide was carried out using different physical separation techniques such as extraction and crystallization followed by drying.

scholarly journals ANTIBACTERIAL ACTIVITY OF TEA, BREADFRUIT, AND PIPER BETLE LEAVES EXTRACTS AGAINST Propionibacterium acnes AND ITS APPLICATION AS ANTI-ACNE MASK

2021 ◽  
Vol 18 (2) ◽  
pp. 105
Author(s):  
Angie Lucita ◽  
Tensiska Tensiska
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Phenolic Content ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Piper Betle ◽  
Bioactive Components ◽  
Disk Diffusion Method ◽  
Extract Concentration

Tea, breadfruit, and piper betle leaves contain bioactive components that have a function as an antibacterial. The purpose of this study was to determine the type of leaf extract that has the highest inhibition power against Proponibacterium acnes and determine the concentration of the selected extract for mask applications that have good physical characteristics and acceptable to panelists. Extraction of tea, breadfruit, and piper betel leaves were carried out with 96% ethanol solvent using maceration method for 24 hours and concentrated with a rotary evaporator. Thick extracts contribute to antibacterial activity by disk diffusion method and total phenolic content. The selected extract was formulated into a clay mask with concentration of 0.5%, 1%, and 1.5%. The result showed that breadfruit leaf extract had the highest inhibition power against Propionibacterium acnes which was 10.844 ± 0.327 mm (strong inhibition category) so that extract can be applied to clay mask. Breadfruit leaf extract concentration of 1.5% was the chosen formulation because it meets the standards, approved by the panelists and the most effective antibacterial for acne problems.

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