scholarly journals Pharmacognostic Evaluation of Ocimum basilicum L

2021 ◽  
pp. 256-261
Author(s):  
R. Anitha ◽  
S. Kanimozhi
Keyword(s):  
Histochemical Study ◽  
Plant Biotechnology ◽  
Fluorescence Analysis ◽  
Ocimum Basilicum ◽  
Plant Biology ◽  
Stomatal Index ◽  
Lower Epidermis ◽  
Organoleptic Evaluation ◽  
Anomocytic Stomata ◽  
Powder Analysis

Aim: Standardization of Ocimum basilicum through pharmacognosy. Place and Duration of Study: Department of Plant Biology and Plant Biotechnology, Ethiraj College for Women, Chennai “Between” Dec 2014-April 15. Methodology: Organoleptic evaluation was carried out based on sensory characters. A free hand anatomical section of the stem was observed. Powder analysis, maceration, Phytochemical test and Fluorescence analysis were conducted according to standard protocol. Results: The organoleptic characters of the dried leaves of Ocimum basilicum were green, aromatic, pungent and brittle in texture with  anomocytic stomata with stomatal index 71.87% in the lower epidermis. A prominent bundle sheath in the leaf was evident. The macerated stem showed annular xylem vessels. The aqueous extract showed the presence of alkaloids, phenols, flavonoids, steroids, terpenoids, and glycosides. Alkaloids and lignins were evident in the histochemical study. Conclusion: Pharmacognostic evaluation of Ocimum basilicum would help in identification, detection of adulterants and development of a monograph.

scholarly journals Pharmacognostic Studies of Putranjiva roxburghii Wall.(Putranjivaeceae)

2018 ◽  
Vol 9 (07) ◽  
Author(s):  
Minj E. ◽  
John Britto S.
Keyword(s):  
Bitter Taste ◽  
Microscopic Analysis ◽  
Fluorescence Analysis ◽  
Vascular Bundles ◽  
Lower Epidermis ◽  
Histochemical Analysis ◽  
Ph Values ◽  
Methanolic Extracts ◽  
Putranjiva Roxburghii ◽  
Powder Analysis

The present investigation focuses on the pharmacognostic features of Putranjiva roxburghii Wall. (Putranjivaeceae). Plants have been studied from different perspectives of macroscopic, microscopic, powder analysis, histochemistry and extractive values. Macroscopic analysis revealed shape, size, odour and taste of leaf, bark, root and seeds. They showed shiny, smooth and rough texture with pungent and bitter taste. Microscopic analysis revealed the presence of upper epidermis, lower epidermis, cortex, vascular bundles, pericycle and pith region of the transverse sections. Histochemical analysis showed the presence of lignins, starch, alkaloids, tannins and calcium oxalate crystals. Saponins are present in leaf, root and stem except in seeds. pH analysis revealed the acidic and basic property of different plant extracts. Leaf, bark, root and seeds extracts showed pH values below 7. Among methanolic extracts, leaf, bark and root showed higher value. Among aqueous extracts root and bark showed higher value. Fluorescence analysis of plants showed different colours in the UV and visible light due to presence of secondary metabolites.

scholarly journals Phyto Pharmacognostic Study of Lagerstroemia speciosa - An Analytical Study

2020 ◽  
Vol 5 (05) ◽  
pp. 206-213
Author(s):  
Jyothi S. Murthy ◽  
B. R. Lalitha ◽  
Aahalya Sharma
Keyword(s):  
Analytical Study ◽  
Microscopical Study ◽  
Water Soluble ◽  
Lower Epidermis ◽  
Calcium Oxalate Crystals ◽  
Phytochemical Study ◽  
Lagerstroemia Speciosa ◽  
Anomocytic Stomata ◽  
Leaf Powder ◽  
Sugar Levels

Lagerstroemia speciosa (L.) Pers (Hindi - Jarul, Taglog - Banaba) a member of Lythraceae family is found all over India, specially in Bengal, Assam and Deccan Peninsula. The leaves of L. speciosa is widely used for lowering blood sugar levels in Philippines, Japan and Taiwan. Pharmacognostical investigation of leaves of L. speciosa was done by evaluating its morphological, microscopical studies, Physicochemical and phytochemical parameters. Rasa Nirdharana was done. Microscopical study revealed presence of epidermal cells contained clusters of rosette aggregate calcium oxalate crystals and few cells were mucilaginous. Lower epidermis showed the presence of anomocytic stomata. Physical constants of leaf powder showed, loss on drying - 3.8%, total ash - 6.7%, acid insoluble ash - 1.039%, water soluble extractive value - 8.88%, alcohol soluble extractive value - 4.49% and pH - 5.75. Preliminary phytochemical study revealed the presence of alkaloids, flavonoids, saponins, triterpenes, tannins, proteins and iron. Rasa Nirdharana confirmed the leaves are Kashaya in Rasa. The Pharmacognostical study was useful for authentication of leaves of Lagerstoemia speciosa.

scholarly journals Macro and microscopic evaluation of Gmelina arborea Roxb. – A botanical pharmacognostic approach for quality control of raw drug material

2020 ◽  
Vol 7 (1) ◽  
pp. 55-60
Author(s):  
R M Vignesh ◽  
V R Sumitha
Keyword(s):  
Quality Control ◽  
Correct Identification ◽  
Efficacy And Safety ◽  
Stomatal Index ◽  
Gmelina Arborea ◽  
Microscopic Evaluation ◽  
Pharmaceutical Industries ◽  
Powder Analysis ◽  
Stomatal Number ◽  
Powdered Drug

Gmelina arborea Roxb. of family Verbenaceae, is one of the highly valued medicinal plant used in numerous traditional medical formulations. It belongs to the ‘Dasamoola’ group of ayurvedic medicinal plants and hence widely exploited. Pharmacognostic techniques involving macroscopic, microscopic and also dry powder analysis serve as botanical methods which help in the correct identification of the crude drug. Leaf constants such as stomatal number, stomatal index, vein islet number, vein termination number also have been determined. Better quality control practices in nutraceutical and pharmaceutical industries demand the correct identification of the dried plants or powdered drug thereby detects and prevents the adulterations, if any. The challenge ahead of this investigation is to validate the therapeutic efficacy and safety of the plant following standard methodology.

scholarly journals Features of the morphological and anatomical characteristics of the leaves of Taraxacum officinale Wigg. in conditions of rock dump

2019 ◽  
Vol 9 (3) ◽  
pp. 78-83
Author(s):  
O. M. Legoshchina ◽  
I. N. Egorova ◽  
O. A. Neverova ◽  
A. A. Bykov ◽  
E. M. Maltseva ◽  
...  
Keyword(s):  
Taraxacum Officinale ◽  
Anatomical Structure ◽  
Stomatal Index ◽  
Lower Epidermis ◽  
Anatomical Features ◽  
Anatomical Changes ◽  
Number Of Leaves ◽  
Positive Correlations ◽  
Number Of Cells ◽  
Anatomical Parameters

The morphological and anatomical features of the assimilation apparatus Taraxacum officinale Wigg., growing under the conditions of a rock dump of the Kedrovsky coal mine in the Kemerovo Region, were studied. It was experimentally established that the specific environmental conditions of the rock dump cause morphological and anatomical changes in the leaves of the dandelion. At the level of leaf morphology, there is a decrease in the average leaf area, thickening of leaf blades, the formation of a trend towards a decrease in the number of leaves in the outlet. At the level of the anatomical structure, there is a significant increase in the thickness of mesophyll, a tendency to a decrease in the thickness of the tissues of the upper and lower epidermis, a decrease in the number of cells of the upper and lower epidermis by 1 mm2 and an increase in the size of stomata in the tissues of the lower and upper epidermis, a decrease in the number of stomata by 1 mm2 and stomatal index on the upper epidermis. Under the conditions of the rock dump, an increase in the number of reliable correlations between the anatomical parameters of the leaf of Taraxacum officinale Wigg., as well as anatomical signs with indicators of atmospheric pollution, was established, to a greater extent due to the formation of positive correlations that are known to control the development of biosystems and are critical for body periods.

scholarly journals PHARMACOGNOSTICAL AND PHYTOCHEMICAL STANDARDIZATION OF PHYSALIS MINIMA L. LEAF

2019 ◽  
pp. 20-25
Author(s):  
DIBYENDU SHIL ◽  
DAMIKI LALOO ◽  
SMRITI REKHA CHANDA DAS ◽  
SUVAKANTA DASH
Keyword(s):  
Tannic Acid ◽  
Physicochemical Parameters ◽  
Standard Procedure ◽  
Fluorescence Analysis ◽  
Volatile Oil ◽  
Protein Quantification ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Anomocytic Stomata ◽  
Solanaceae Family

Objective: To evaluate the pharmacognostical and phytochemical parameters of Physalis minima leaf. Methods: The leaf of Physalis minima was examined for macroscopical, microscopical, physicochemical parameters and fluorescence analysis. Extracts obtained from the leaf was analyzed for phytochemical screening and estimation of total tannin, phenolic and flavonoid content following the standard procedure available in the literature. Results: Morphologically, the leaf was found to be ovate in shape, 5 to 8 cm long and up to 3 cm width with dentate margin, asymmetrical base, hairy surface, reticulate veins on each side of midrib, green in colour, characteristic odour and slight bitter in taste. Microscopic study has shown the important diagnostic characters of Solanaceae family which is characterized by presence of dorsiventral leaf with anomocytic stomata, grandular or uniseriate trichomes and cluster crystal of calcium oxalate. Physicochemical parameters like foreign organic matter (0.78%), loss on drying (8.23%), total ash (11.4%), acid insoluble ash (2.2%), water-soluble ash (6.1%), sulphated ash (2.4%) alcohol soluble extractive (10.4%), water-soluble extractive (9.5%), ether soluble extractive (1.8%) foaming index (below 100), swelling index (1), volatile oil and heavy metal content were quantified. Phytochemical analysis of different extracts of Physalis minima leaf has shown the presence of phytoconstituents viz. alkaloids, steroids, tannin, flavonoids, protein. Quantification of phytoconstituents was also reported like phenols (10.59±0.65 mg/gm equivalent to tannic acid), tannin (8.24±0.27 mg/gm equivalent to tannic acid) and flavonoids (87.17±0.87 mg/gm equivalent to rutin) respectively. Conclusion: This present study was provided the qualitative and quantitative standard of Physalis minima will help to prevent the possible steps of adulteration with other species of the same genus.

scholarly journals Pharmacognostic and Preliminary Phytochemical Evaluation of Leaf of Syzygium Cumini (L.) Skeels

2021 ◽  
Vol 12 (3) ◽  
pp. 684-688
Author(s):  
Shyam Baboo Prasad ◽  
Arun M Gurav ◽  
Goli P Prasad ◽  
Anupam K Mangal ◽  
Srikanth N
Keyword(s):  
Single Layer ◽  
Fluorescence Analysis ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Syzygium Cumini ◽  
Lower Epidermis ◽  
Microscopic Evaluation ◽  
Physicochemical Studies ◽  
Physicochemical Evaluation

Syzygium cumini (L.) Skeels (Family: Myricaceae) commonly known as Jamun is a widely used medicinal plant in Ayurveda. In spite of its numerous medicinal attributes, no published work is available on pharmacognostic characterization, physicochemical analysis of its leaves. The measures taken for pharmacognostic characterization were macroscopy, microscopy, powder microscopy, physicochemical evaluation, fluorescence analysis, and preliminary phytochemical screening. The microscopic evaluation showed useful characters for the recognition of leaf of Syzygium cumini (L.) Skeels . Transverse section, showed presence of single layer of wavy epidermal cells with striated cuticle single layer Palisade beneath upper epidermis in lamina region (contains compact elongated cells), Spheraphide in lamina region, collenchyma below the upper epidermis and above the lower epidermis in midrib region, Xylem and phloem in center, Sclerenchyma in between vascular bundle and collenchyma in midrib region. Wavy epidermal cell in the upper epidermis, anisocytic stomata in the lower epidermis, Bordered pitted reticulate vessels, Calcium oxalate crystal sheath in the lamina, fragment of fibers and Spiral vessels were observed in powder microscopy. Physicochemical studies showed total ash (3.1%), acid insoluble ash (0.7%), alcohol soluble extractive values (10.96 %), and water-soluble extractive values (12.32 %). Phytochemical analysis revealed the presence of sugar, lipid, Glycoside, saponins, phenols, flavonoids, tannins, tri-terpenoids and steroids. The study forms the first report on pharmacognostic characters and a physicochemical parameter which could be useful for identification and authentication of the plant.

scholarly journals Comparative analysis of the leaf anatomy of Echinacea purpurea and E. pallida

2018 ◽  
Vol 26 (2) ◽  
pp. 77-84
Author(s):  
T. N. Belaeva ◽  
A. N. Butenkova
Keyword(s):  
Comparative Analysis ◽  
Vascular Bundle ◽  
Echinacea Purpurea ◽  
Botanical Garden ◽  
Stomatal Index ◽  
Lower Epidermis ◽  
Southeastern Part ◽  
Stomatal Complex ◽  
Mean Values ◽  
The Mean

This article contains a comparative analysis of the anatomical structure of the rosette and stem leaves of Echinacea purpurea and E. pallida. The plants were grown on the experimental site of the Siberian Botanical Garden, located in the southeastern part of Tomsk (Western Siberia). Temporary preparations of the leaves were prepared by cutting them on a freezing microtome MZ-2. Photographs of leaf microscopy and microscopic measurements were made on a light microscope. Anomocyte type of stomatal complex is characteristic for the studied species. Leaves of the studied species are amphistomatic, rosette leaves of E. purpurea are hypostomatic. The stomatal index of E. purpurea was 1.5 (stem leaves, upper epidermis) and 24.4–25.5 (lower epidermis). In the leaf blade of E. purpurea, the signs are revealed as mesomorphic (the presence of relatively large dorsoventral leaves, moderate development of integumentary, conducting and mechanical tissues) and a xeromorphic-heliomorphic structure. A complex of xeromorphic-heliophytic features of the E. pallida leaf has been identified, which increase its drought resistance; the mean values of the stomatal index of the upper and lower epidermis were 24.8–28.7. There are 10 characteristics that have significant differences in mean values when comparing leaves of E. purpurea and E. pallida: the size of the stomata of the lower epidermis, the thickness of the mesophyll of the leaf, its layers and the leaf laminae free of the vascular bundle, the area of the vascular bundle and their constituents.

scholarly journals Plants Used for Ethno-veterinary Practices by Tribes of Kambakkam of Eastern Ghats

2021 ◽  
Vol 70 (1) ◽  
Author(s):  
Ettiappan Munuswamya ◽  
Karmegam Nandhagopala ◽  
S. Surabia ◽  
Sivagamasundari b ◽  
Manoranjithamc M. ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Andhra Pradesh ◽  
Standard Procedure ◽  
Plant Biotechnology ◽  
Plant Biology ◽  
Eastern Ghats ◽  
Future Perspective ◽  
Herbarium Specimens ◽  
Government College ◽  
Medicine Development

The study reports the surveyed of medicinal plants used by of village Tribe people of Kambakkam Forest (Easternghats), Andhra Pradesh in ethno-veterinary practices. During this study information about the ethno-veterinary plants were collected and preserved as herbarium specimens by follows the standard procedure. The plants were identified with help of some standard Floras. The specimens were deposited in the herbarium of Department of Plant Biology and Plant Biotechnology, Loganatha Narayanasamy Government College, Ponneri, Thiruvallur District. During the survey it was noted 23 plants were traditionally used by various animal diseases such as Inflammation, wound healing, indigestion, dysentery, fever, swelling, bone fracture, diarrhea, cold, cough, pneumonia, constipation, antimicrobial and milk yielding properties. The information provided in this study would bring new medicine development of ecofriedly, effective medicines to control human diseases in the future perspective. This study may be useful to protect and conserve the medicinal plants of Kambakkam Forest.

An x-ray microprobe based upon a close-coupled focal-spot / glass capillary arrangement

1992 ◽  
Vol 50 (2) ◽  
pp. 1758-1759
Author(s):  
D. A. Carpenter ◽  
M. A. Taylor
Keyword(s):  
Imaging Techniques ◽  
Fluorescence Analysis ◽  
High Sensitivity ◽  
Specimen Preparation ◽  
X Rays ◽  
Glass Capillary ◽  
Close Coupling ◽  
X Ray ◽  
Point To Point ◽  
Beam Damage

The development of intense sources of x rays has led to renewed interest in the use of microbeams of x rays in x-ray fluorescence analysis. Sparks pointed out that the use of x rays as a probe offered the advantages of high sensitivity, low detection limits, low beam damage, and large penetration depths with minimal specimen preparation or perturbation. In addition, the option of air operation provided special advantages for examination of hydrated systems or for nondestructive microanalysis of large specimens.The disadvantages of synchrotron sources prompted the development of laboratory-based instrumentation with various schemes to maximize the beam flux while maintaining small point-to-point resolution. Nichols and Ryon developed a microprobe using a rotating anode source and a modified microdiffractometer. Cross and Wherry showed that by close-coupling the x-ray source, specimen, and detector, good intensities could be obtained for beam sizes between 30 and 100μm. More importantly, both groups combined specimen scanning with modern imaging techniques for rapid element mapping.

XRMF applications of a monolithic, polycapillary, focusing x-ray optic

1996 ◽  
Vol 54 ◽  
pp. 240-241
Author(s):  
D. A. Carpenter ◽  
Ning Gao ◽  
G. J. Havrilla
Keyword(s):  
Trace Elements ◽  
Point Source ◽  
Focal Spot ◽  
Fluorescence Analysis ◽  
X Rays ◽  
Focal Distance ◽  
Spot Diameter ◽  
X Ray ◽  
Focal Spot Diameter

A monolithic, polycapillary, x-ray optic was adapted to a laboratory-based x-ray microprobe to evaluate the potential of the optic for x-ray micro fluorescence analysis. The polycapillary was capable of collecting x-rays over a 6 degree angle from a point source and focusing them to a spot approximately 40 µm diameter. The high intensities expected from this capillary should be useful for determining and mapping minor to trace elements in materials. Fig. 1 shows a sketch of the capillary with important dimensions.The microprobe had previously been used with straight and with tapered monocapillaries. Alignment of the monocapillaries with the focal spot was accomplished by electromagnetically scanning the focal spot over the beveled anode. With the polycapillary it was also necessary to manually adjust the distance between the focal spot and the polycapillary.The focal distance and focal spot diameter of the polycapillary were determined from a series of edge scans.

Sign in / Sign up

Export Citation Format

Share Document