scholarly journals Poultry Farms as a Potential Source of Environmental Pollution by Pharmaceuticals

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1031 ◽  
Author(s):  
Katarzyna Wychodnik ◽  
Grażyna Gałęzowska ◽  
Justyna Rogowska ◽  
Marta Potrykus ◽  
Alina Plenis ◽  
...  
Keyword(s):  
High Performance ◽  
Bacterial Resistance ◽  
Soil Samples ◽  
Reference Area ◽  
Bacterial Isolates ◽  
Mass Spectrometry Detection ◽  
Veterinary Pharmaceuticals ◽  
Potential Source ◽  
Poultry Breeding ◽  
Increase Productivity

Industrial poultry breeding is associated with the need to increase productivity while maintaining low meat prices. Little is known about its impact on the environment of soil pollution by pharmaceuticals. Breeders routinely use veterinary pharmaceuticals for therapeutic and preventive purposes. The aim of this work was to determine the influence of mass breeding of hens on the soil contamination with 26 pharmaceuticals and caffeine. During two seasons—winter and summer 2019—15 soil samples were collected. Liquid extraction was used to isolate analytes from samples. Extracts were analyzed using ultra-high performance liquid chromatography coupled with tandem mass spectrometry detection (UPLC-MS/MS). The results showed the seasonal changes in pharmaceutical presence in analyzed soil samples. Ten pharmaceuticals (metoclopramide, sulphanilamide, salicic acid, metoprolol, sulphamethazine, nimesulide, carbamazepine, trimethoprim, propranolol, and paracetamol) and caffeine were determined in soil samples collected in March, and five pharmaceuticals (metoclopramide, sulphanilamide, sulphamethazine, carbamazepine, sulfanilamid) in soil samples collected in July. The highest concentrations were observed for sulphanilamide, in a range from 746.57 ± 15.61 ng/g d.w to 3518.22 ± 146.05 ng/g d.w. The level of bacterial resistance to antibiotics did not differ between samples coming from intensive breeding farm surroundings and the reference area, based on antibiotic resistance of 85 random bacterial isolates.

scholarly journals Flocculating Properties of a Bioflocculant Purified from Bacillus Subtilis Isolated from the Stream Sediments of Onyearugbulem Market, Akure, Nigeria

2019 ◽  
pp. 1-16
Author(s):  
F. O. Ekundayo ◽  
F. B. Omiyale ◽  
E. R. Omomo
Keyword(s):  
Bacillus Subtilis ◽  
Metal Ions ◽  
Bacillus Anthracis ◽  
Streptomyces Griseus ◽  
Stream Sediments ◽  
Soil Samples ◽  
Bacterial Isolates ◽  
Potential Source ◽  
Flocculating Activity ◽  
Ondo State

Soil samples (sediments of stream, its bank and abattoir soil) were collected from Onyearugbulem market abattoir, Akure, Ondo State, Nigeria. Bacteria were isolated from the above soil samples by dilution and pour plate methods. Screening for best bioflocculating bacteria was also performed. Effects of metal ions (such as Mg2+, Ca2+ and Al3+), temperature and pH on flocculating activities of the bioflocculant were also determined. Six bacterial isolates producing flocculating substances were isolated and the isolate with the best flocculating property was selected. The identified bioflocculant producing bacteria are Bacillus anthracis, B. subtilis, B. thuringiensis, B. cereus, Streptomyces griseus and S. somaliensis. The best bioflocculant producing bacterium was Bacillus subtilis and the flocculating activity of its bioflocculant was stimulated in the presence of Mg2+, Ca2+and Al3+. This bioflocculant was thermostable and retained more than 80% of its flocculating activity after being heated at 100ºC for 25 minutes. It had the highest flocculating activity of 85% at pH 6 with optimum bioflocculant dosage of 0.8 mL. This study suggests soil samples from Onyearugbulem market abattoir as a potential source of bioflocculant-producing bacteria with good bioflocculating properties.

Impurities from polypropylene microcentrifuge tubes as a potential source of interference in simultaneous analysis of multiple lipid-soluble antioxidants by HPLC with electrochemical detection

2004 ◽  
Vol 42 (4) ◽  
Author(s):  
Hsiu-Chuan Yen ◽  
Yi-Tzu Hsu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Hplc Analysis ◽  
Coenzyme Q ◽  
Retinyl Palmitate ◽  
Plasma Samples ◽  
Potential Source ◽  
Effect Of Impurities ◽  
Β Carotene

AbstractHigh-performance liquid chromatography (HPLC) with electrochemical detection was used to analyze lipidsoluble antioxidants and micronutrients in plasma. Small amounts of plasma samples are often extracted in polypropylene (PP) microcentrifuge tubes before HPLC analysis due to its convenience. We therefore investigated the effect of impurities released from different PP tubes during extraction on the electrochemical detection of retinol, lutein, α-tocopherol, γ-tocopherol, retinyl palmitate, β-carotene and total coenzyme Q

scholarly journals INDENTIFIKASI HIGH PERFORMANCE WORK SYSTEM

2020 ◽  
Vol 1 (2) ◽  
pp. 108-118
Author(s):  
I Nengah Aristana ◽  
I Wayan Arta Artana
Keyword(s):  
High Performance ◽  
Work Systems ◽  
Business World ◽  
High Performance Work Systems ◽  
Task Delegation ◽  
Work System ◽  
Analysis Techniques ◽  
Increase Productivity ◽  
High Performance Work System ◽  
And Performance

The development of the business world now requires all forms of business including cooperatives to do various ways to increase productivity and performance. One of the efforts in increasing productivity and performance is by building a high-performance work system. The purpose of this research is to find out the high performance work system in cooperatives. The number of respondents was 132 respondents with factor analysis analysis techniques. From the results of the analysis conducted found three factors as determinants of high performance work systems, namely the method of task delegation, internal planning and motivation.

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

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