scholarly journals Effect of Bacillus subtilis QM3 on β-amylase Isoenzyme in Early Germination of Wheat Seed

2020 ◽  
pp. 24-32
Author(s):  
Ya-Jing Li ◽  
Qing-Ping Hu
Keyword(s):  
Bacillus Subtilis ◽  
Seed Germination ◽  
Plant Growth ◽  
Polyacrylamide Gel Electrophoresis ◽  
Early Period ◽  
Stress Factors ◽  
Wheat Seed ◽  
Early Germination ◽  
Growth Promoting ◽  
Wheat Seeds

Seed germination is the basis and initial stage in the process of plant growth and development. Bacillus subtilis QM3 is a plant growth promoting rhizobacterium (PGPR) with function of growth promoting, promoting, prevention of pathogens and pests attack and can improve plant resistance to different stress factors. Through the measurement of the early germination rate of wheat (Triticum aestivam L.). seeds under B. subtilis QM3 treatment, the results showed that B. subtilis QM3 can significantly promote the germination of wheat seeds, which has reached a significant level at 6h after seeds sowing or at 6 h of treatment, during imbibition. The β-amylase isoenzyme in early period of wheat seed germination was measured by denaturing polyacrylamide gel electrophoresis. The results showed that the band width and brightness of β-amylase isoenzyme of wheat seeds treated with B. subtilis QM3 increased during three to 6 h of imbibition and especially the effect was significant at 6 h. In the early period, the band of α-amylase isoenzyme could not be detected. It is suggested that the increase of β-amylase isoenzyme early band may be one of the main reasons for B. subtilis QM3 to promote wheat seed germination. Through the combination of B. subtilis QM3 with free and binding states of β-amylase, it was found that the former can increase the activity of β-amylase by either increasing free β-amylase or releasing binding β-amylaseisoenzyme. β-amylaseisoenzyme inhibitors can significantly inhibit β-amylase activity, nevertheless α-amylase activators and inhibitors have no significant effect on β-amylaseisoenzyme, which further proved that the β-amylase exerted the effects in the early period of wheat seed more precisely during the imbibition of the seeds.

scholarly journals Studying of the promotion mechanism of Bacillus subtilis QM3 on wheat seed germination based on β-amylase

2020 ◽  
Vol 15 (1) ◽  
pp. 553-560
Author(s):  
Ya-Jing Li ◽  
Qing-Ping Hu
Keyword(s):  
Bacillus Subtilis ◽  
Seed Germination ◽  
Early Stage ◽  
Control Group ◽  
Wheat Seed ◽  
Early Germination ◽  
Amylase Isoenzymes ◽  
Promotion Mechanism ◽  
Wheat Seeds

AbstractThe response of β-amylase in early stage germination of wheat seeds to Bacillus subtilis QM3 was mainly focused on to elucidate the promotion mechanism of B. subtilis QM3. The results showed that the changes in apparent activity of amylase and endosperm liquefaction after the strain QM3 treatment were much more obvious than that of the control group; the activity of β-amylase treated with the different concentrations of the strain QM3 increased significantly (P < 0.05) by 4% (107 CFU/mL) and 18.5% (106 CFU/mL) at the germination 6 h. Moreover, after presoaking with α-cyclodextrin, the activity of β-amylase increased significantly (P < 0.05) by 18.5% (107 CFU/mL) and 23.4% (106 CFU/mL) at the same stage of germination; the electrophoretogram of β-amylase isoenzymes showed that there is a growing trend in brightness and width of the band during the early germination from 3 to 6 h of wheat seed treated by the strain QM3 (106 CFU/mL). The increase in activity and isoenzyme expression of β-amylase may be one of the important reasons to promote the germination of wheat seeds after being treated by B. subtilis QM3.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals The Effectiveness of Giving Plant PGPR Rhizosphere Bamboo on Cocoa Seeds Germination at The Nursery Level

2021 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Muhammad Yusril Hardiansyah ◽  
Yunus Musa ◽  
Abdul Mollah Jaya
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Block Design ◽  
Plant Growth Promoting Rhizobacteria ◽  
Special Treatment ◽  
Initial Growth ◽  
Seed Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

The low productivity of cocoa plantations in Indonesia is partly due to the low quality of seeds, which refers to the impeded growth of cultivated cocoa nurseries. Seed is the initial growth of plants so the importance of giving special treatment to seeds will refer to better seed growth. Provision of Plant Growth Promoting Rhizobacteria (PGPR) microbes can produce indoleacetic acid (IAA) in plants to improve the quality of plant growth. This study aims to determine the effectiveness of the provision of Plant Growth Promoting Rhizobacteria bamboo rhizosphere against cocoa seed germination. The study was carried out in the farmer group garden, Gantarangkeke District, Bantaeng. This study was arranged in the form of a two-factor factorial design (F2F) in a randomized block design (RBD). The use of cocoa seed type as the first factor consisted of GTB (Gantarangkeke Bantaeng) local cocoa seed and MCC 01 cocoa seed and seed immersion treatment at PGPR rhizosphere bamboo concentration as the second factor consisting of 0% (control) concentration, 5%, 10 % and 15%. The results obtained indicate that administration of seeds with bamboo rhizosphere PGPR affects the germination (100.00%), the speed of seed growth (7.14%/etmal), as well as on abnormal seeds (10.00%). So that the provision of bamboo rhizosphere PGPR on cocoa seeds has an effective influence on seed germination and cocoa seedling development.

scholarly journals Quorum quenching activity of the PGPR Bacillus subtilis UD1022 alters nodulation efficiency of Sinorhizobium meliloti on Medicago truncatula

2020 ◽  
Author(s):  
Amanda Rosier ◽  
Pascale B. Beauregard ◽  
Harsh P. Bais
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Quorum Quenching ◽  
Agricultural System ◽  
Synergistic Activity ◽  
Sustainable Solutions ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPlant growth promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a ‘consortia’ approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530 and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model ‘consortium’. These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.

Performance of plant growth-promoting bacterium of duckweed under different kinds of abiotic stress factors

2019 ◽  
Vol 19 ◽  
pp. 101146 ◽  
Author(s):  
Hidehiro Ishizawa ◽  
Minami Tada ◽  
Masashi Kuroda ◽  
Daisuke Inoue ◽  
Michihiko Ike
Keyword(s):  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Factors ◽  
Abiotic Stress Factors ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Evaluation of the plant-growth-promoting abilities of endophytic bacteria from the psammophyteAmmodendron bifolium

2018 ◽  
Vol 64 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Yanlei Zhu ◽  
Xiaoping She
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Growth Stages ◽  
Bacterial Isolates ◽  
Pellicle Formation ◽  
Radicle Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The objective of this study was to assess the plant-growth-promoting abilities of 45 endophytic bacterial isolates from Ammodendron bifolium through physiological characteristics detection and endophytic bacteria–plant interaction. Each of these isolates exhibited 1 or more plant-growth-promoting traits, but only 11 isolates belonging to the genera Bacillus, Staphylococcus, and Kocuria were capable of promoting seed germination and radicle growth. These results together with the results of the correlation analysis revealed that the completion of seed germination may not be due to IAA production, phosphate solubilization, pellicle formation, and ACC deaminase, protease and lipase production by endophytic bacteria, but may be closely related to amylase and cellulase production. Further, endophytic bacterial isolates with plant-growth-promoting traits may also provide beneficial effects to host plants at different growth stages. Thus, these results are of value for understanding the ecological roles of endophytic bacteria in host plant habitats and can serve as a foundation for further studies of their potential in plant regeneration.

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