CROP WATER STRESS INDEX FOR PREDICTING YIELD LOSS IN COMMON BEAN

The crop water stress index (CWSI), an index derived from canopy temperature, has been widely studied as a physiological indicator of plant water status to optimize irrigation in common beans. However, it is not clear how this index could contribute to yield prediction as a decision support tool in irrigation management. This paper aimed to use the CWSI for predicting yield loss in common bean (Phaseolus vulgaris L.) subjected to water stress under drip irrigation. A rain shelter experiment was conducted using a completely randomized design with five replications. The indeterminate growth cultivar TAA Dama was subjected to three irrigation treatments: 100% of the field capacity (FC), 75 and 50% FC from 20 days after sowing (DAS) until the end of the crop cycle. Grain yield was reduced by 42% under 50% FC treatment. Furthermore, stomatal conductance was reduced under this treatment, whereas the CWSI and canopy temperature increased as irrigation levels decreased. The relationship between grain yield and CWSI (R2=0.76, RSME=2.35g) suggests that canopy temperature data could be used to forecast grain yield losses. In conclusion, farmers can have a low-cost, effective technique for making water management decisions in common bean.

Kaolin Film Increases Gas Exchange Parameters of Coffee Seedlings During Transference From Nursery to Full Sunlight

Increases in water use efficiency (WUE) and the reduction of negative impacts of high temperatures associated with high solar radiation are being achieved with the application of fine particle film of calcined and purified kaolin (KF) on the leaves and fruits of various plant species. KF was applied on young Coffea arabica and Coffea canephora plants before their transition from nursery to full sunlight during autumn and summer. The effects of KF were evaluated through the responses of leaf temperature (Tleaf), net CO2 assimilation rate (A), stomatal conductance (gs), transpiration (E), WUE, crop water stress index (CWSI), index of relative stomatal conductance (Ig), initial fluorescence (F0), and photosynthetic index (PI) in the first 2–3 weeks after the plant transitions to the full sun. All measurements were performed at midday. In Coffea plants, KF decreased the Tleaf up to 6.7°C/5.6°C and reduced the CWSI. The plants that were not protected with KF showed lower A, gs, E, and Ig than those protected with KF. C. canephora plants protected with KF achieved higher WUE compared with those not protected by 11.23% in autumn and 95.58% in summer. In both Coffea sp., KF application reduced F0, indicating reduced physical dissociation of the PSII reaction centers from the light-harvesting system, which was supported with increased PI. The use of KF can be recommended as a management strategy in the transition of Coffea seedlings from the nursery shade to the full sunlight, to protect leaves against the excessive solar radiation and high temperatures, especially in C. canephora during the summer.

Determination of Irrigation Time Using Plant Water Stress Index Values of Second Crop Sunflower in Semi-Arid Climate Conditions

This study was carried out in 2020 to determine crop water stress index (CWSI) by using infrared thermometer (IRT) data calculated by leaf canopy temperature measurements of the second crop sunflower genotype in semi-arid climate conditions, and to determine the relationships between irrigation time, seed yield of sunflower plant and CWSI by using these index values. Irrigation program consisted of a full irrigation and 2 different levels of stress, which were 100% (I100), 70% (I70), 35% (I35) of water losses within the effective root depth of 90 cm every 7 days. A total of 644 mm of irrigation water was applied to I100 (control) irrigation. The water consumption for full irrigation was 721 mm and the yield was 3516.00 kg/ha. Lower limit (LL) value without water stress required to determine plant water stress index was Tc-Ta=-2.528×VPD +0.749 (R2=0.814) and upper limit (UL) value, where the plant is completely under water stress, was determined as +3.27℃. Crop water stress index value threshold at which sunflower seed yield started to decrease was calculated as 0.33 using the infrared thermometer measurements at the time of irrigation. In addition, a negative correlation was obtained between sunflower seed yield and CWSI values. The results revealed that the yield tends to decrease as the CWSI increases.

High-throughput phenotyping for drought tolerance in rice

Most lowland rice in West Africa depends mainly on rainfall for water supply. Drought is consequently one of the major constraints on rice production, drastically affecting both plant growth and development. The objective of this work was to study the impact of water deficit both on canopy temperature and on chlorophyll fluorescence level, used as indicators of transpiration and photosynthetic activity. Measurements using infrared thermography and fluorimetry were taken on both 17 lines resulting from the cross IR64 X B6144F-MR-6-0-0 and their two parents plus one tolerant (APO) controls. These 20 lines were phenotyped after applying a drought constraint in a controlled laboratory environment in Montpellier (France) in 2013 and - 2014 and in field in the lowlands of Banfora and Farako-ba (INERA Burkina Faso) in 2014. Results showed that the drought stress sustained by the plants increased canopy temperature in all lines, entailing differential disturbance of the photosynthetic process, markedly depressed in susceptible lines. A classification of the lines with respect to their sensitivity to stress could be established by using the Drought Factor Index (DFI), and Crop Water Stress Index (CWSI) as was established a correlation between the phenotyping methods by infrared thermography and fluorimetry. This article propose an efficient application of combined imaging as a rapid and accurate phenotyping tool for crop yield improvement, in particular by monitoring the efficiency of plant responses to the fluctuating of environmental conditions. This study proved the efficiency of the method combining IR thermographie and fluorimetry as a field phenotyping tools for drought resistance.

The Application of a Self-Organizing Model for the Estimation of Crop Water Stress Index (CWSI) in Soybean with Different Watering Levels

A field experiment was conducted with soybean to observe evapotranspiration (ET) and crop water stress index (CWSI) with three watering levels at Keszthely, Hungary, during the growing seasons 2017–2020. The three different watering levels were rainfed, unlimited, and water stress in flowering. Traditional and converted evapotranspirometers documented water stress levels in two soybean varieties (Sinara, Sigalia), with differing water demands. ET totals with no significant differences between varieties varied from 291.9 to 694.9 mm in dry, and from 205.5 to 615.6 mm in wet seasons. Theoretical CWSI, CWSIt was computed using the method of Jackson. One of the seasons, the wet 2020 had to be excluded from the CWSIt analysis because of uncertain canopy temperature, Tc data. Seasonal mean CWSIt and Tc were inversely related to water use efficiency. An unsupervised Kohonen self-organizing map (K-SOM) was developed to predict the CWSI, CWSIp based on easily accessible meteorological variables and Tc. In the prediction, the CWSIp of three watering levels and two varieties covered a wide range of index values. The results suggest that CWSIp modelling with the minimum amount of input data provided opportunity for reliable CWSIp predictions in every water treatment (R2 = 0.935–0.953; RMSE = 0.033–0.068 mm, MAE = 0.026–0.158, NSE = 0.336–0.901, SI = 0.095–0.182) that could be useful in water stress management of soybean. However, highly variable weather conditions in the mild continental climate of Hungary might limit the potential of CWSI application. The results in the study suggest that a less than 450 mm seasonal precipitation caused yield reduction. Therefore, a 100–160 mm additional water use could be recommended during the dry growing seasons of the country. The 150 year-long local meteorological data indicated that 6 growing seasons out of 10 are short of precipitation in rainfed soybean.

Crop Water Stress Index as a Proxy of Phenotyping Maize Performance under Combined Water and Salt Stress

The crop water stress index (CWSI), based on canopy temperature (Tc), has been widely used in evaluating plant water status and planning irrigation scheduling, but whether CWSI can diagnose the stress status of crops and predict the physiological traits and growth under combined water and salt stress remains to be further studied. Here, a model of CWSI was established based on the continuous measurements of Tc for two maize genotypes (ZD958 and XY335) under two water and salt conditions, combined with growth stage-specific non-water-stressed baselines (NWSB). The relationships between physiology, growth, and yield of maize with CWSI were analyzed. There were significant differences in NWSB between the two maize genotypes at the same and different growth stages; thus, growth stage-specific NWSBs were used. The difference in NWSB was due to the difference and change in effective leaf width. CWSI was closely related to leaf water potential, stomatal conductance, and net photosynthetic rate under different water and salt stress, and also explained the variations in leaf area index, biomass, water use, and yield. Collectively, CWSI can be used as a proxy indicator of high-throughput phenotyping maize performance under combined water and salt stress, which will be valuable for predicting yield and improving water use efficiency.

Simulating Canopy Temperature Using a Random Forest Model to Calculate the Crop Water Stress Index of Chinese Brassica

The determination of crop water status has positive effects on the Chinese Brassica industry and irrigation decisions. Drought can decrease the production of Chinese Brassica, whereas over-irrigation can waste water. It is desirable to schedule irrigation when the crop suffers from water stress. In this study, a random forest model was developed using sample data derived from meteorological measurements including air temperature (Ta), relative humidity (RH), wind speed (WS), and photosynthetic active radiation (Par) to predict the lower baseline (Twet) and upper baseline (Tdry) canopy temperatures for Chinese Brassica from 27 November to 31 December 2020 (E1) and from 25 May to 20 June 2021 (E2). Crop water stress index (CWSI) values were determined based on the predicted canopy temperature and used to assess the crop water status. The study demonstrated the viability of using a random forest model to forecast Twet and Tdry. The coefficients of determination (R2) in E1 were 0.90 and 0.88 for development and 0.80 and 0.77 for validation, respectively. The R2 values in E2 were 0.91 and 0.89 for development and 0.83 and 0.80 for validation, respectively. Our results reveal that the measured and predicted CWSI values had similar R2 values related to stomatal conductance (~0.5 in E1, ~0.6 in E2), whereas the CWSI showed a poor correlation with transpiration rate (~0.25 in E1, ~0.2 in E2). Finally, the methodology used to calculate the daily CWSI for Chinese Brassica in this study showed that both Twet and Tdry, which require frequent measuring and design experiment due to the trial site and condition changes, have the potential to simulate environmental parameters and can therefore be applied to conveniently calculate the CWSI.

Long-Term Assessment of Reference Baselines for the Determination of the Crop Water Stress Index in Maize under Mediterranean Conditions

Canopy temperature has been proposed as a relevant variable for crop water stress monitoring. Since crop temperature is highly influenced by the prevailing climatic conditions, it is usually normalized with indices such as the crop water stress index (CWSI). The index requires the use of two baselines that relate canopy temperature under maximum stress and non-water stress conditions with vapor pressure deficit (VPD). These reference baselines are specific to each crop and climatic region. In maize, they have been extensively studied for certain climatic regions but very little is known on their suitability to be used under Mediterranean-type conditions nor their temporal stability, both diurnally and between seasons. Thus, the objective of this work was to determine the reference baselines for maize grown under Mediterranean conditions, as well as its diurnal and long-term stability. An experiment was conducted for 3 years in a maize breeding field, under well-watered and water-stressed irrigation treatments. The determined reference baselines for computing CWSI in maize have shown to be stable in the long term but markedly influenced by the meteorological variations between 10–17 h UTC (Coordinated Universal Time). These results indicate that several reference baselines should be used for CWSI computing throughout the abovementioned time interval. The CWSI values calculated for well-watered and water-stressed maize breeding plots using the reference baselines derived in this study were successfully correlated with other physiological indicators of plant water stress.

Detecting Plant Stress Using Thermal and Optical Imagery From an Unoccupied Aerial Vehicle

Soil and water salinization has global impact on the sustainability of agricultural production, affecting the health and condition of staple crops and reducing potential yields. Identifying or developing salt-tolerant varieties of commercial crops is a potential pathway to enhance food and water security and deliver on the global demand for an increase in food supplies. Our study focuses on a phenotyping experiment that was designed to establish the influence of salinity stress on a diversity panel of the wild tomato species, Solanum pimpinellifolium. Here, we explore how unoccupied aerial vehicles (UAVs) equipped with both an optical and thermal infrared camera can be used to map and monitor plant temperature (Tp) changes in response to applied salinity stress. An object-based image analysis approach was developed to delineate individual tomato plants, while a green–red vegetation index derived from calibrated red, green, and blue (RGB) optical data allowed the discrimination of vegetation from the soil background. Tp was retrieved simultaneously from the co-mounted thermal camera, with Tp deviation from the ambient temperature and its change across time used as a potential indication of stress. Results showed that Tp differences between salt-treated and control plants were detectable across the five separate UAV campaigns undertaken during the field experiment. Using a simple statistical approach, we show that crop water stress index values greater than 0.36 indicated conditions of plant stress. The optimum period to collect UAV-based Tp for identifying plant stress was found between fruit formation and ripening. Preliminary results also indicate that UAV-based Tp may be used to detect plant stress before it is visually apparent, although further research with more frequent image collections and field observations is required. Our findings provide a tool to accelerate field phenotyping to identify salt-resistant germplasm and may allow farmers to alleviate yield losses through early detection of plant stress via management interventions.

Identification of Infiltration Features and Hydraulic Properties of Soils Based on Crop Water Stress Derived from Remotely Sensed Data

Knowledge of the spatial variability of soil hydraulic properties is important for many reasons, e.g., for soil erosion protection, or the assessment of surface and subsurface runoff. Nowadays, precision agriculture is gaining importance for which knowledge of soil hydraulic properties is essential, especially when it comes to the optimization of nitrogen fertilization. The present work aimed to exploit the ability of vegetation cover to identify the spatial variability of soil hydraulic properties through the expression of water stress. The assessment of the spatial distribution of saturated soil hydraulic conductivity (Ks) and field water capacity (FWC) was based on a combination of ground-based measurements and thermal and hyperspectral airborne imaging data. The crop water stress index (CWSI) was used as an indicator of crop water stress to assess the hydraulic properties of the soil. Supplementary vegetation indices were used. The support vector regression (SVR) method was used to estimate soil hydraulic properties from aerial data. Data analysis showed that the approach estimated Ks with good results (R2 = 0.77) for stands with developed crop water stress. The regression coefficient values for estimation of FWC for topsoil (0–0.3 m) ranged from R2 = 0.38 to R2 = 0.99. The differences within the study sites of the FWC estimations were higher for the subsoil layer (0.3–0.6 m). R2 values ranged from 0.12 to 0.99. Several factors affect the quality of the soil hydraulic features estimation, such as crop water stress development, condition of the crops, period and time of imaging, etc. The above approach is useful for practical applications for its relative simplicity, especially in precision agriculture.