Antibacterial, Antifungal, and Cytotoxic Activity of Excoecaria agallocha Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

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Antiplasmodial and cytotoxic activity of isolated sesquiterpene lactones from the acetone leaf extract of Vernonia colorata

South African Journal of Botany ◽

10.1016/j.sajb.2008.10.001 ◽

2009 ◽

Vol 75 (1) ◽

pp. 176-179 ◽

Cited By ~ 21

Author(s):

J.C. Chukwujekwu ◽

C.A. Lategan ◽

P.J. Smith ◽

F.R. Van Heerden ◽

J. Van Staden

Keyword(s):

Cytotoxic Activity ◽

Leaf Extract ◽

Sesquiterpene Lactones

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In vitro Antioxidant Analysis and the DNA Damage Protective Activity of Leaf Extract of the Excoecaria agallocha Linn Mangrove Plant

Agricultural Chemistry ◽

10.5772/55416 ◽

2013 ◽

Cited By ~ 1

Author(s):

C. Asha ◽

M.S. Resmi ◽

E.V. Soniy

Keyword(s):

Dna Damage ◽

Leaf Extract ◽

Protective Activity ◽

Mangrove Plant ◽

Excoecaria Agallocha ◽

Dna Damage Protective

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Phytochemical screening and evaluation of cytotoxic activity of Calotropis gigantea leaf extract on MCF7, HeLa, and A549 cancer cell lines

Journal of Natural Science Biology and Medicine ◽

10.4103/jnsbm.jnsbm_215_18 ◽

2019 ◽

Vol 10 (2) ◽

pp. 131 ◽

Cited By ~ 1

Author(s):

Venkataraman Prabhu ◽

Bindu Damodaran ◽

Prashantha Nagaraja ◽

Vivek Jain ◽

MP Manuja Viraj Wimalasiri ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Leaf Extract ◽

Cancer Cell Lines ◽

Phytochemical Screening ◽

Calotropis Gigantea

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Alkaloid Cytotoxic Test on Ethanol Extract from Itchy Leaves (Laportea decumana (Roxb.) Wedd.)

10.46610/rapls.2021.v03i02.001 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Septiani Mangiwa ◽

Septiani Mangiwa

Keyword(s):

Cytotoxic Activity ◽

Total Alkaloid ◽

Leaf Extract ◽

Gravimetric Method ◽

Ethanol Extract ◽

Artemia Salina ◽

Test Results ◽

Pain Reliever ◽

Cytotoxic Test ◽

Extraction Stage

The Itchy leaf [Laportea decumana (Roxb.) Wedd] is a plant that is widely used as traditional medicine by Papuans as a pain reliever. The purpose of this study was to determine the total level of alkaloids in itchy leaf extract and to test the cytotoxic activity using the BSLT method. The research series began with the extraction stage with the maceration method using 96% ethanol, which tested the alkaloid level. The test was carried out using the gravimetric method and the cytotoxic test using the alkaloid level test results using the BSLT method on shrimp larvae (Artemia salina L). The results showed that itchy leaf extract contained a total alkaloid level of 6.46%. Cytotoxic testing using the BSLT method showed an LC50 of 54.33 ppm and was found to be toxic.

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Antimicrobial, Antioxidant and Cytotoxic Activity of Silver Nanoparticles Synthesized by Leaf Extract of Erythrina suberosa (Roxb.)

Frontiers in Molecular Biosciences ◽

10.3389/fmolb.2017.00014 ◽

2017 ◽

Vol 4 ◽

Cited By ~ 64

Author(s):

Yugal K. Mohanta ◽

Sujogya K. Panda ◽

Rasu Jayabalan ◽

Nanaocha Sharma ◽

Akshaya K. Bastia ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cytotoxic Activity ◽

Leaf Extract

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Cytotoxicity, analgesic and anthelmintic studies of the different plant parts of Saraca indica Linn. (Family: Caesalpiniaceae)

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20163967 ◽

2016 ◽

Vol 2 (11) ◽

pp. 278

Author(s):

Most. Nazma Parvin

Keyword(s):

Cytotoxic Activity ◽

Analgesic Activity ◽

Leaf Extract ◽

Methanol Extract ◽

Anthelmintic Activity ◽

Bark Extract ◽

Tail Flick ◽

Standard Drug ◽

Vincristine Sulphate ◽

Analgesic Activities

Background: Saraca indica or Saraca asoca belonging to the family Caesalpiniaceae. It is popularly known as Ashoka, is the most ancient tree of Bangladesh. This versatile plant has anti-cancer, anti-menorrhagic, anti-oxytocic, anti-microbial activity and has extended uses in ayurveda, unani and homeopathy. The present study attempts to investigate the cytotoxic and analgesic activities of the methanol extract of bark and the anthelmintic activity of the leaf extract of Saraca indica.Methods: The cytotoxic activity of the methanol extract of the bark was performed by brine shrimp lethality bioassay and analgesic potential of the bark extract was evaluated using both acetic acid induced writhing test and tail-flick tests to understand the central analgesic activity using Swiss Albino mice as experimental animal. Anthelmintic activity of the leaf extract was performed by observing the time of paralysis and the time of death of earthworms, Pheretima posthuma. Results: The methanol extract of the bark showed moderate cytotoxic activity when compared with the standard drug vincristine sulphate. The results of analgesic activities suggested that the methanol extract of the bark possess significant analgesic activity in mice, which is comparable to the standard drug, diclofenac-Na. The fresh juice extract of the leaf of S. indica showed potent anthelmintic activity when compared with standard drug, albendazole.Conclusions: Our present study, demonstrates the cytotoxic, analgesic and anthelmintic, properties of Saraca indica, which validate its use in traditional medicine.

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GEL ANTI JERAWAT EKSTRAK DAUN BUTA-BUTA (Excoecaria agallocha L.) DAN PENGUJIAN ANTIBAKTERI Staphylococcus epidermidis

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2015.v1.i2.6215 ◽

2015 ◽

Vol 1 (2) ◽

pp. 65-72

Author(s):

Ika Olivia Borman ◽

Yusriadi Yusriadi ◽

Evi Sulastri

Keyword(s):

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Leaf Extract ◽

Inhibition Zone ◽

Positive Control ◽

Diffusion Method ◽

Activity Data ◽

Excoecaria Agallocha ◽

Significant Difference ◽

The Stability

Buta-Buta (Excoecaria agallocha L.) leaf is a type of mangrove plants that has antibacterial activity againtsbacteriacausing acne, Staphylococcus epidermidis,. This study aims to formulate buta-buta leaf extract with the concentration of 12.5 % into anti-acne gel using two gels, namely HPMC 8% (Formula 1) and Carbopol 940 2%(Formula 2). Antibacterial activity test was carried out by agar diffusion method using well. The stability and antibacterial activity data during 28 days were analyzed statistically using unpaired t-student with a degree of confidence of 95% (α = 0.05). The evaluation results of anti-acne gel form of buta-buta leaf extract in Formula 2 showedbetter characteristics than Formula 1, which include organoleptic examination, homogenity, pH and viscosity during 28 days. The antibacterial test showed the diameter of inhibition zone of Formula 2 is 11.03 mm ±0,01. The statistic analysis showed a significant difference between Formula 2 with a positive control (klindamisin gel).

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